Journal
JOURNAL OF RADIATION RESEARCH
Volume 59, Issue 2, Pages 101-107Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jrr/rrx071
Keywords
boron neutron capture therapy; accelerator-based neutron source; lithium target; boric acid; in vitro efficacy evaluation
Funding
- Russian Science Foundation [14-32-00006]
- Budker Institute of Nuclear Physics SB RAS
- Institute of Molecular and Cell Biology SB RAS
- Novosibirsk State University
- Japanese Ministry of Education, Culture, Sports, Science and Technology [17K15797, 26293320H]
- Russian Science Foundation [17-32-00001] Funding Source: Russian Science Foundation
- Grants-in-Aid for Scientific Research [26293320, 17K15797] Funding Source: KAKEN
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In the current article, we provide in vitro efficacy evaluation of a unique accelerator-based neutron source, constructed at the Budker Institute of Nuclear Physics (Novosibirsk, Russian Federation), for boron neutron capture therapy (BNCT), which is particularly effective in the case of invasive cancers. U251MG, CHO-K1 and V79 cells were incubated and irradiated in various concentrations of boric acid with epithermal neutrons for 2-3 h in a plexiglass phantom, using 2.0 MeV proton energy and 1.5-3.0 mA proton current, resulting in a neutron fluence of 2.16 x 10(12) cm(-2). The survival curves of cells loaded with boron were normalized to those irradiated without boron (to exclude the influence of the fast neutron and gamma dose components) and fit to the linear-quadratic (LQ) model. Colony formation assays showed the following cell survival rates (means +/- SDs): CHO-K1: 0.348 +/- 0.069 (10 ppm), 0.058 +/- 0.017 (20 ppm), 0.018 +/- 0.005 (40 ppm); V79: 0.476 +/- 0.160 (10 ppm), 0.346 +/- 0.053 (20 ppm), 0.078 +/- 0.015 (40 ppm); and U251MG: 0.311 +/- 0.061 (10 ppm), 0.131 +/- 0.022 (20 ppm), 0.020 +/- 0.010 (40 ppm). The difference between treated cells and controls was significant in all cases (P < 0.01) and confirmed that the neutron source and irradiation regimen were sufficient for control over cell colony formation. We believe our study will serve as a model for ongoing in vitro experiments on neutron capture therapy to advance in this area for further development of accelerator-based BNCT into the clinical phase.
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