Correction
Biochemical Research Methods
Marco Ruiz-Campos, Libia Sanz, Fabian Bonilla, Mahmood Sasa, Bruno Lomonte, Fausto Zaruma-Torres, Maria Teran, Julian Fernandez, Juan J. Calvete, Cleopatra A. S. Caldeira, Saulo L. Da Silva
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Barbara S. Soares, Surza Lucia G. Rocha, Viviane A. Bastos, Diogo B. Lima, Paulo C. Carvalho, Fabio C. Gozzo, Borries Demeler, Tayler L. Williams, Janelle Arnold, Amy Henrickson, Thomas J. D. Jorgensen, Tatiana A. C. B. Souza, Jonas Perales, Richard H. Valente, Bruno Lomonte, Francisco Gomes-Neto, Ana Gisele C. Neves-Ferreira
Summary: It has been discovered that DM64, an antitoxin from Didelphis aurita, can neutralize snake venom toxicity by interacting with myotoxic phospholipases A(2). This study investigated the noncovalent complex formed between DM64 and myotoxin II, and revealed the interaction mechanism using various techniques such as molecular modeling and molecular dynamics simulations.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Toxicology
Rosimeire Silva-Carvalho, Matheus Z. Gaspar, Luiz H. B. Quadros, Luis G. G. Lobo, Leticia M. Rogerio, Najla T. S. Santos, Maria C. Zerbinatti, Cecilia L. Santarem, Elisangela O. Silva, Juliana R. Gerez, Nelson J. Silva Jr, Bruno Lomonte, Edward G. Rowan, Rafael S. Floriano
Summary: In this study, the researchers investigated the effects of varespladib alone or in combination with a coral snake antivenom on the local and systemic effects caused by Micrurus corallinus venom in rats. They found that varespladib, when used in combination with antivenom, effectively prevented neurotoxic, nephrotoxic, and inflammatory activities induced by the venom, showing a synergistic effect.
TOXICOLOGY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Josselin A. Hernandez-Altamirano, David Salazar-Valenzuela, Evencio J. Medina-Villamizar, Diego R. Quirola, Ketan Patel, Sakthivel Vaiyapuri, Bruno Lomonte, Jose R. Almeida
Summary: This study investigated the composition patterns of two coral snake venoms from Ecuador and found interspecific variability. The results provide a comprehensive view of the composition of these venoms and expand the knowledge of Micrurus venom phenotypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biodiversity Conservation
Leonardo De Oliveira, Felipe Gobbi Grazziotin, Paola Maria Sanchez-Martinez, Mahmood Sasa, Oscar Flores-Villela, Ana Lucia Da Costa Prudente, Hussam Zaher
Summary: A new study reveals that goo-eating snakes have a unique venom delivery system that relies on their lower jaw instead of upper lip and maxillary glands. This change likely occurred in the ancestor of goo-eating snakes, possibly due to the loss of the embryonic posterior maxillary lamina.
SYSTEMATICS AND BIODIVERSITY
(2023)
Article
Pharmacology & Pharmacy
Diana Mora-Obando, Bruno Lomonte, Davinia Pla, Jimmy Alexander Guerrero-Vargas, Santiago Ayerbe-Gonzalez, Jose Maria Gutierrez, Mahmood Sasa, Juan J. Calvete
Summary: Snake venoms are complex biological mixtures that contain proteins, peptides, and nucleotides. They are produced in specialized glands and have evolved over millions of years to serve defense, immobilization, and digestion purposes. The study of the variability in venom composition has become an important area of research in toxinology.
Article
Food Science & Technology
Carlos Correa-Netto, Marcelo A. Strauch, Marcos Monteiro-Machado, Ricardo Teixeira-Araujo, Juliana Guzzo Fonseca, Moema Leitao-Araujo, Maria Lucia Machado-Alves, Libia Sanz, Juan J. Calvete, Paulo A. Melo, Russolina Benedeta Zingali
Summary: Polyclonal antibodies have been the only scientifically validated treatment for snakebites for over a century, but there have been moderate innovations in this immunotherapy. New strategies and approaches are being explored to develop next-generation antivenoms.
Review
Spectroscopy
Juan J. Calvete, Bruno Lomonte, Anthony J. Saviola, Francisco Calderon Celis, Jorge Ruiz Encinar
Summary: The advent of soft ionization mass spectrometry-based proteomics in the 1990s allowed for integral analysis of whole proteomes. However, the analytical technique itself is inherently nonquantitative, leading to the development of strategies for proteomic quantification. The combination of elemental and molecular mass spectrometry systems holds potential for absolute quantification in various areas of proteomics.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Juan J. Calvete, Bruno Lomonte, Carolina Lorente, Davinia Pla, Michael Zollweg, Dietrich Mebs
Summary: Based on its relationship to other reptiles, the Chinese crocodile lizard has been classified as venomous. However, no data about its oral secretion have been reported. This study compared the mandibular gland of the Chinese crocodile lizard with a herbivorous lizard and found no evidence of venom-derived peptides or proteins, supporting the non-venomous nature of these lizards.
Review
Pharmacology & Pharmacy
Bruno Lomonte
Summary: Muscle necrosis is a potential complication of snakebite envenomings, caused by myotoxins in snake venom that directly damage skeletal muscle fibers. Among these myotoxins, Lys49 sPLA2-like proteins are able to induce muscle necrosis by a mechanism independent of phospholipid hydrolysis. This review aims to comprehensively summarize the historical developments and current knowledge regarding the mechanism of action of Lys49 sPLA2-like myotoxins, identifying gaps for future research to improve snakebite treatments.
Article
Infectious Diseases
Milena Mechkarska, Taylor S. Cunning, Megan G. Taggart, Nigel G. Ternan, Jerome Leprince, Laurent Coquet, Thierry Jouenne, Jordi Tena-Garces, Juan J. Calvete, J. Michael Conlon
Summary: The venom of the Trinidad thick-tailed scorpion contains peptides with antimicrobial activity and potassium channel and sodium channel toxins. Among the peptides, TtAP-1 exhibits strong bactericidal activity against various bacteria and can rapidly kill clinically relevant bacteria. However, its high hemolytic activity limits its potential as an anti-infective agent.
Review
Cell Biology
Alfredo Jesus Lopez-Davila, Bruno Lomonte, Jose Maria Gutierrez
Summary: Snakebite envenoming can lead to skeletal muscle necrosis, and this review focuses on the mechanisms behind it. The toxins in snake venom disrupt the sarcolemma, causing an influx of calcium ions and subsequent degeneration of the muscle fibers. However, experiments suggest that the hypercontraction of muscle fibers is not a direct effect of the toxins, but rather a result of increased cytosolic calcium due to sarcolemmal damage, which may contribute to muscle damage by generating mechanical stress and further sarcolemmal damage.
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
(2023)
Article
Parasitology
Cecilia Diaz, Marcela Serna-Gonzalez, Arturo Chang-Castillo, Bruno Lomonte, Fabian Bonilla, Adriana Alfaro-Chinchilla, Felipe Triana, Mahmood Sasa
Summary: The objective of this study was to examine the differences in venom peptide sequences of Tityus species in Costa Rican rainforests compared to T. cf. asthenes from Colombia. The results showed that the venom peptides of dark-colored Tityus in Costa Rica are similar to those found in other T. (Atreus) species from the region. This suggests that the level of toxicity caused by Tityus in Costa Rica should be similar to that caused by members of the same subgenus in other geographical locations. The lack of epidemiological evidence of medical emergencies caused by envenoming by this scorpion group in Costa Rica may be more related to ecological and demographic factors.
Article
Biology
Fabian Bonilla, Juan S. Monros, Mahmood Sasa
Summary: Environmental compensation is a legal/technical tool that can reduce biodiversity loss and improve environmental services quality caused by infrastructure projects or development activities. It employs various approaches such as economic, ecological, political, and cultural to establish compensatory actions and plays a crucial role in environmental management. However, clear procedures and close vigilance are necessary for its practical application.
REVISTA DE BIOLOGIA TROPICAL
(2022)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)