Review
Nutrition & Dietetics
Moganatharsa Ganeshalingam, Samantha Enstad, Sarbattama Sen, Sukhinder Cheema, Flavia Esposito, Raymond Thomas
Summary: Breast milk is the best source of nutrition for infants, with functional lipids playing a crucial role. Lipidomics, an emerging field, helps to determine the physiological mechanisms of lipids and discover biomarkers for early disease diagnosis.
FRONTIERS IN NUTRITION
(2022)
Article
Agriculture, Multidisciplinary
Lili Zhao, Jinxuan Zhang, Wupeng Ge, Jun Wang
Summary: The lipidomes of human milk and ruminant milk were compared in this study. The results showed that human milk was closer to camel milk in terms of lipid composition, with similar digestion and bioactivity properties. Camel milk could be considered as a new resource for lipid base in infant formula.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jayashree Selvalatchmanan, A. V. Rukmini, Shanshan Ji, Alexander Triebl, Liang Gao, Anne K. Bendt, Markus R. Wenk, Joshua J. Gooley, Federico Torta
Summary: The study validated a method for extracting lipids from human breastmilk and found that 85% of lipid species showed stable inter-individual differences across time points. Some lipids exhibited higher concentrations in the evening compared with the morning, with diacylglycerols and triacylglycerols showing greater variability in the evening samples.
Article
Food Science & Technology
Shuangshuang Wang, Yuxuan Song, Rui He, Guanli Du, Lei Zhang, Bangjun Zhang, Jiaying Zhang, Lili Zhao, Jing Zhang, Wupeng Ge
Summary: In this study, a comprehensive lipidomic analysis was conducted to characterize polar lipids in mature breast milk and ewe milk. A total of 362 polar lipid species were identified, and 139 significantly differentially expressed lipids were screened between the two milk types. PE (16:1_18:0) was found to be significantly higher in ewe milk compared to breast milk. Sphingolipid metabolism and glycerophospholipid metabolism were determined as vital metabolic pathways.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Danjie Wu, Le Zhang, Yan Zhang, Jiachen Shi, Chin Ping Tan, Zhaojun Zheng, Yuanfa Liu
Summary: Using lipidomics, the researchers analyzed and compared the lipid patterns of human milk (HM) and infant formulas (IFs) and found significant differences in the lipid compositions. HM was rich in unsaturated and long-chain fatty acids, while IFs mainly consisted of saturated and medium-chain fatty acids. The glycerophospholipid metabolism pathway was found to be the most significant. These findings provide valuable insights for the development of Chinese infant formulas.
Article
Chemistry, Applied
Yixuan Tan, Jiafei Liu, Yang Yang, Xufeng Wang, Xiaomeng Sun, Jianjun Cheng
Summary: This study analyzed the lipid composition of raw goat milk and homogenized goat milk using LC-ESI-MS/MS and identified 56 different lipids. Phosphatidylcholines (PCs) decreased after homogenization, while lysophosphatidylcholines (LPCs) increased. These findings provide a detailed understanding of the impact of homogenization on goat milk lipids.
Article
Chemistry, Analytical
Leicheng Zhang, Tengfei Xu, Jingtao Zhang, Stephen Choong Chee Wong, Mark Ritchie, Han Wei Hou, Yulan Wang
Summary: The study proposes a novel strategy integrating spiral inertial microfluidics and ion mobility mass spectrometry for single-cell metabolite detection and identification. The technology successfully screened the metabolic profiles of different types of cancer cells, improving confidence in metabolite identification. This approach offers a simple and efficient method for single-cell lipid profiling, with additional ion mobility separation significantly enhancing the confidence toward identification of metabolites.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Yixuan Tan, Junli Hao, Yuhang Jiang, Xiaomeng Sun, Jianjun Cheng
Summary: The effects of pasteurization and spray drying on goat milk lipids were evaluated using LC-ESI-MS/MS and multiple variable statistics. A total of 1061 lipids assigned to 29 subclasses were identified, and 185 different lipids were selected as markers for different treatments. Glycerophospholipids were the most affected subclasses, especially by spray drying. Five potential lipid markers were used to distinguish different treated goat milk, and glycerophospholipid metabolism was the primary pathway of these markers. These results provide more details of lipid profiles in thermally treated goat milk.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Marcela Konjevod, Jorge Saiz, Matea Nikolac Perkovic, Gordana Nedic Erjavec, Lucija Tudor, Suzana Uzun, Oliver Kozumplik, Coral Barbas, Neven Zarkovic, Nela Pivac, Dubravka Svob Strac
Summary: Posttraumatic stress disorder (PTSD) is a complex neuropsychiatric disorder triggered by a traumatic event. This study used lipidomic analysis to identify altered lipid compounds in PTSD patients. The identified lipids are associated with biological processes such as membrane integrity and function, mitochondrial dysfunction, inflammation, and oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Chemistry, Applied
Qian Liu, Junying Zhao, Yan Liu, Weicang Qiao, Tiemin Jiang, Yan Liu, Xiaowen Yu, Lijun Chen
Summary: The differences in composition and distribution of fatty acids make human milk lipids distinct from those of other mammals. Understanding the concentrations, compositions, and distribution characteristics of milk lipids requires analysis and detection technology. The characteristics and mimicking of human milk lipids have been extensively studied to reduce the gap between human milk and infant formulae.
Article
Neurosciences
Kourosh Hooshmand, Jin Xu, Anja Hviid Simonsen, Asger Wretlind, Andressa de Zawadzki, Karolina Sulek, Steen Gregers Hasselbalch, Cristina Legido-Quigley
Summary: This study developed a robust lipidomics and metabolomics method based on commonly used two-phase extraction systems for exploring biochemical changes in neurodegenerative diseases. The aB&D method was found to be the most suitable for lipid extraction from human CSF samples. In combination with multiple chromatographical separation approaches, a comprehensive metabolite signature of human CSF samples was achieved.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Xiuxiu Zhang, Lu Liu, Lina Wang, Yue Pan, Xinyue Hao, Gengxu Zhang, Xiaodong Li, Muhammad Hussain
Summary: The study analyzed and compared the lipidome and fatty acid composition of human milk and infant formulas with different fat sources, finding common lipid species but significant variations in fatty acid composition, especially depending on the fat source used in the formulas. Overall, the results provide valuable insights for designing new milk formulas that better replicate human milk.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Environmental Sciences
Santosh Lamichhane, Heli Siljander, Daniel Duberg, Jarno Honkanen, Suvi M. Virtanen, Matej Oresic, Mikael Knip, Tuulia Hyotylainen
Summary: This study found that maternal exposure to PFAS affects the lipid composition of breast milk, which in turn has detrimental impacts on infant growth and intestinal health.
ENVIRONMENT INTERNATIONAL
(2021)
Article
Biochemical Research Methods
Dorota Garwolinska, Michal Mlynarczyk, Agata Kot-Wasik, Weronika Hewelt-Belka
Summary: The study found that storage of human milk samples at 4 degrees Celsius for 4 days and subjecting them to three freeze-thaw cycles can lead to changes in lipid content. Differences in levels of certain lipid species between samples stored at -20 degrees Celsius and -80 degrees Celsius were not statistically significant, and individual variance remained consistent regardless of sample storage conditions. Storing HM samples at -20 degrees Celsius for up to 3 weeks and at -80 degrees Celsius for up to 12 weeks ensures sample lipidome stability.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Analytical
Tian Xia, Ming Yuan, Yongwei Xu, Feng Zhou, Kate Yu, Yu Xia
Summary: Glycerolipids are crucial for cellular lipid homeostasis, with dysregulation in their metabolism linked to metabolic diseases. A new workflow utilizing charge-tagging PB derivatization, SFC, and MS/MS has been developed to accurately analyze GL structures and composition, potentially enabling lipid marker discovery for disease monitoring or diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Monika Wieczerzak, Jacek Namiesnik, Blazej Kudlak
Summary: This study focused on the genotoxicity of pharmaceuticals from different therapeutic groups, both in mixtures and individual solutions, under different environmental conditions. The results showed that the addition of inorganic ions mainly reduced genotoxicity, while changes in the pH of pharmaceutical solutions significantly affected genotoxicity. Synergistic interactions were more commonly observed among mixtures.
DRUG AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Emilia Waraksa, Katarzyna Owczarek, Pawel Kubica, Ewa Klodzinska, Mariusz Ozimek, Robert Wrzesien, Barbara Bobrowska-Korczak, Jacek Namiesnik
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2020)
Article
Nutrition & Dietetics
Dorota Garwolinska, Weronika Hewelt-Belka, Agata Kot-Wasik, Ulrik Kraemer Sundekilde
Article
Nutrition & Dietetics
Weronika Hewelt-Belka, Dorota Garwolinska, Michal Mlynarczyk, Agata Kot-Wasik
Article
Biochemical Research Methods
Mateusz Kacper Wozniak, Laura Banaszkiewicz, Justyna Aszyk, Marek Wiergowski, Iwona Janczewska, Jolanta Wierzba, Agata Kot-Wasik, Marek Biziuk
Summary: This study developed a method for the simultaneous extraction of FAEEs, EtG, and EtS from one meconium aliquot, demonstrating sensitivity, accuracy, efficiency, and the potential for reduced organic solvent usage compared to existing methods.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Barbara Kusznierewicz, Marika Mroz, Izabela Koss-Mikolajczyk, Jacek Namiesnik
Summary: This study presents methods for assessing the health quality of products containing betalains, comparing data on phytochemical composition of beetroot cultivars using various techniques. Identified that betalain contributes about 50% to the antioxidant activity in red beetroot. Photometric measurements are recommended for simple analysis of betacyanin and betaxanthin content, while liquid chromatography techniques provide more precise information. The combination of liquid chromatography with high-resolution mass spectrometry is recommended for more detailed metabolomics studies, identifying sixty-four phytochemicals in beetroot samples.
Article
Multidisciplinary Sciences
Monika Baranowska, Zuzanna Koziara, Klaudia Suliborska, Wojciech Chrzanowski, Michael Wormstone, Jacek Namiesnik, Agnieszka Bartoszek
Summary: The study demonstrates the significant impact of interactions between common food flavonoids on their electrochemical properties and bioactivities. Mixtures of flavonoids show enhanced antioxidant properties compared to individual components, with a synergistic effect observed in both chemical and cellular tests. In cellular tests, the mixtures exhibit properties of a new substance rather than those of individual components, stimulating cell growth.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Dorota Garwolinska, Michal Mlynarczyk, Agata Kot-Wasik, Weronika Hewelt-Belka
Summary: The study found that storage of human milk samples at 4 degrees Celsius for 4 days and subjecting them to three freeze-thaw cycles can lead to changes in lipid content. Differences in levels of certain lipid species between samples stored at -20 degrees Celsius and -80 degrees Celsius were not statistically significant, and individual variance remained consistent regardless of sample storage conditions. Storing HM samples at -20 degrees Celsius for up to 3 weeks and at -80 degrees Celsius for up to 12 weeks ensures sample lipidome stability.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Microbiology
Gloria Griffante, Weronika Hewelt-Belka, Camilla Albano, Francesca Gugliesi, Selina Pasquero, Sergio Fernando Castillo Pacheco, Greta Bajetto, Paolo Ettore Porporato, Erica Mina, Marta Vallino, Christian Krapp, Martin Roelsgaard Jakobsen, John Purdy, Jens von Einem, Santo Landolfo, Valentina Dell'Oste, Matteo Biolatti
Summary: Human cytomegalovirus (HCMV) utilizes host cell resources for new virion assembly, including inducing cellular metabolism changes to favor virion production. However, the host defense factor IFI16 can counteract HCMV-induced metabolic reprogramming, limiting virion infectivity by downregulating glucose transporter 4 and reducing lipogenic enzyme transcription. This discovery unveils a potential target for novel antiviral therapies.
Article
Chemistry, Applied
Inal Bakhytkyzy, Weronika Hewelt-Belka, Agata Kot-Wasik
Summary: This study demonstrates a new method for comprehensive flaxseed lipidome profiling using LC-Q-TOF-MS and dispersive micro-solid-phase extraction. The method allows for simultaneous monitoring of both polar and non-polar lipids, overcoming the challenge of lipid species abundance.
Review
Biochemistry & Molecular Biology
Dorota Garwolinska, Agata Kot-Wasik, Weronika Hewelt-Belka
Summary: Metabolomics is widely used in various fields such as clinical diagnostics, disease investigation, therapy prediction, treatment efficiency monitoring, drug discovery, and sample composition analysis. The study design, including careful selection of conditions and consideration of pre-analytical variability, is crucial for robust and reliable results. This study provides key information on pre-analytical variables affecting metabolomics studies of biological fluids, serving as a practical review and identifying factors that introduce artificial data variation.
Article
Chemistry, Applied
Inal Bakhytkyzy, Weronika Hewelt-Belka, Agata Kot-Wasik
Summary: Growing public health awareness has led to increased demand for natural products with positive health benefits. This study conducted a comprehensive analysis and comparison of the lipid profiles of seven oilseeds using D-mu-SPE and LC-Q-TOF-MS. The results showed that flax, hemp, and chia seeds had similar lipid content, while black cumin, black and white sesame seeds had a similar lipid composition with more saturated fatty acid substituents. ALA and LA were present in all lipid classes.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Meeting Abstract
Dermatology
Adrian Kobiela, Weronika Hewelt-Belka, Joanna E. Frackowiak, Natalia Kordulewska, Lilit Hovhannisyan, Aleksandra Bogucka, Rachel Etherington, Artur Pirog, Irena Dapic, Susanne Gabrielsson, Sara Brown, Graham S. Ogg, Danuta Gutowska-Owsiak
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Weronika Hewelt-Belka, Michal Mlynarczyk, Dorota Garwolinska, Agata Kot-Wasik
Summary: This study used reversed-phase liquid chromatography coupled to mass spectrometry to evaluate the molecular distribution of GM3 in human milk samples. The results showed that the types and content of GM3 varied across different lactation stages. These findings contribute to our understanding of the dynamic composition of human milk.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
