Article
Biochemistry & Molecular Biology
Wenfeng Deng, Xiaodi Wang, Lina Chen, Bolun Wen, Yunshan Chen, Kaiyuan Ji, Huishu Liu
Summary: Myometrial-derived exosomes contain specific proteins and miRNAs that may play a role in intercellular communication and myometrial function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Victor Lozano, Carla Martin, Noelia Blanco, Ignacio Alcalde, Luis Fernandez-Vega Cueto, Jesus Merayo-Lloves, Luis M. Quiros
Summary: This article examines the abnormalities of exosomes in keratoconus and suggests their influence on the development of the pathology. It also highlights the therapeutic potential of using exosomes from healthy individuals.
Article
Biotechnology & Applied Microbiology
Christoph Keysberg, Oliver Hertel, Louise Schelletter, Tobias Busche, Chiara Sochart, Jorn Kalinowski, Raimund Hoffrogge, Kerstin Otte, Thomas Noll
Summary: CHO cells derived from Chinese hamster ovaries are widely used in biopharmaceutical production, but research on exosomes in these cells is scarce. Exosomes are considered a universal mediator of intercellular communication and a proposed new drug delivery format in biopharmaceuticals. This study analyzed the proteome and RNA of exosomes from CHO-K1 cells, providing reference data for future research.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Immunology
Lifei Liang, Xiaoqing Xu, Jiawei Li, Cheng Yang
Summary: This review summarizes and discusses the bidirectional regulation between miRNAs and MDSCs in the tumor microenvironment, revealing the impact of miRNAs and MDSCs on tumor immune escape and metastasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Yeojin Hong, Anh Duc Truong, Jiae Lee, Thi Hao Vu, Sooyeon Lee, Ki-Duk Song, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study identified significantly differentially expressed miRNAs in resistant chickens compared to susceptible chickens after H5N1 infection, with a focus on regulation of immune-related genes, particularly the MAPK pathway molecules. The results showed higher miRNA expression levels in resistant chickens than susceptible chickens, indicating a potential for using exosomal miRNA expression as biomarkers for disease resistance against HPAIV H5N1.
VETERINARY RESEARCH
(2021)
Article
Endocrinology & Metabolism
Tian Fang, Gong Xue, Wu Jianjun, Long Wei, Zhang Xiaomeng, Yang Fan
Summary: This study integrated transcriptome data and lncRNA regulatory association data of human pancreatic islet-derived exosome to construct a ceRNA network. It identified potential markers and mechanisms of diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Microbiology
Zhicheng Ji, Li Ma
Summary: In analyzing metatranscriptomics data, it is important to control for both DNA and taxa abundances to eliminate their strong correlation with RNA abundance.
Article
Chemistry, Multidisciplinary
Gijung Kwak, Jing Cheng, Hyosuk Kim, Sukyung Song, Su Jin Lee, Yoosoo Yang, Ji Hoon Jeong, Ji Eun Lee, Phillip B. Messersmith, Sun Hwa Kim
Summary: In this study, a hydrolytically degradable PEG hydrogel-based exosome delivery system was designed to induce macrophages to transition from proinflammatory to anti-inflammatory state, promoting cutaneous wound healing.
Article
Clinical Neurology
Juhong Zhang, Haizhu Ma, Guijun Yang, Jing Ke, Wenfang Sun, Li Yang, Shaojing Kuang, Hai Li, Wei Yuan
Summary: This study compared the miRNA profiles of serum-derived exosomes in patients with SSNHL and normal hearing controls to find miRNAs associated with SSNHL or biomarkers for SSNHL. Exosomes were isolated from blood samples and identified using various techniques. 18 differentially expressed miRNAs were found in SSNHL cases, and functional analysis revealed their association with important signaling pathways. PC-5p-38556_39, PC-5p-29163_54, and miR-93-3p were identified as potential biomarkers for SSNHL. The study concludes that miRNAs in exosomes may play a role in the pathogenesis of SSNHL.
FRONTIERS IN NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Jiang Guo, Xingang Zhou, Long Cheng, Xuesong Gao
Summary: This study aimed to construct a miRNA-mRNA network to elucidate the molecular mechanism of exosome function in metastatic HCC. Through analyzing RNA samples from 50 cases, 149 DEMs and 60 DEGs involved in the progression of metastatic HCC were identified and a miRNA-mRNA network related to exosomes was constructed. The low expression of NUCKS1 in HCC was validated, and HCC patients with low expression of NUCKS1 had shorter overall survival. The findings provide new insights into the underlying molecular mechanisms of exosomes in metastatic HCC and suggest NUCKS1 as a potential therapeutic target.
Article
Environmental Sciences
Keng Po Lai, Nathan Yi Kan Tam, Yuelong Chen, Chi Tim Leung, Xiao Lin, Chau Fong Tsang, Yin Cheung Kwok, William Ka Fai Tse, Shuk Han Cheng, Ting Fung Chan, Richard Yuen Chong Kong
Summary: Recent studies have shown that hypoxia affects sex differentiation and reproductive function in fish, leading to a male-biased population. The molecular mechanisms through which hypoxia alters fish sex differentiation and sex ratios are still poorly understood. In this study, we conducted sequencing of small RNAs and transcripts in marine medaka embryos exposed to hypoxia, and identified dysregulated miRNAs and mRNAs. Our findings suggest that miRNA-mRNA interactions may play a role in regulating embryonic development and sex determination in response to hypoxic stress.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Fisheries
Tina Kollannoor Johny, Rinu Madhu Puthusseri, Sarita Ganapathy Bhat
Summary: The fish gut is a complex ecosystem influenced by various factors, and the gut microbiota plays important roles in the physiological functions of the host. With technological advancements, the application of culture-independent techniques in studying fish gut microbiota is promising for a better understanding.
AQUACULTURE RESEARCH
(2021)
Article
Oncology
Hongqiang Yao, Siriguleng Yu, Yuchen Luo, Ming Wang, Xiuying Wang, Siriguleng Xu, Yufei Chen, Zhifeng Xie
Summary: This study investigated the effects of Bactrian camel plasma-derived exosomes on hepatocellular carcinoma (HCC). The results showed that exosomes from normal Bactrian camels can inhibit the growth of HCC cells by regulating multiple signaling pathways.
FRONTIERS IN ONCOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Anh Duc Truong, Suyeon Kang, Hoang Vu Dang, Yeojin Hong, Thi Hao Vu, Jubi Heo, Nhu Thi Chu, Huyen Thi Nguyen, Ha Thi Thanh Tran, Yeong Ho Hong
Summary: In this study, 27 differentially expressed miRNAs were identified in the serum of African swine fever virus (ASFV)-infected pigs. These miRNAs were found to be associated with immune response, virus-host interactions, and several viral genes. This study provides important insights into the pathogenesis of ASFV and the host's immune response during an acute infection.
JOURNAL OF ANIMAL SCIENCE
(2023)
Review
Oncology
Benjamin Duran-Vinet, Karla Araya-Castro, Juan Calderon, Luis Vergara, Helga Weber, Javier Retamales, Paulina Araya-Castro, Pamela Leal-Rojas
Summary: Colorectal cancer is a prevalent cancer with high mortality rates, often diagnosed late with low survival rates. CRISPR/Cas-based molecular platforms show promise in detecting cancer-derived exosome micro-RNAs for early CRC diagnosis, presenting a cost-effective and efficient approach to improve patient outcomes.
Article
Immunology
Dallas A. Banks, Sarah E. Ahlbrand, V. Keith Hughitt, Swati Shah, Katrin D. Mayer-Barber, Stefanie N. Vogel, Najib M. El-Sayed, Volker Briken
JOURNAL OF IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Rebecca J. Edgar, Vincent P. van Hensbergen, Alessandro Ruda, Andrew G. Turner, Pan Deng, Yoann Le Breton, Najib M. El-Sayed, Ashton T. Belew, Kevin S. McIver, Alastair G. McEwan, Andrew J. Morris, Gerard Lambeau, Markj Walker, Jeffrey S. Rush, Konstantin Korotkov, Goran Widmalm, Nina M. van Sorge, Natalia Korotkova
NATURE CHEMICAL BIOLOGY
(2019)
Article
Biochemical Research Methods
Dapeng Chen, Lucia Geis-Asteggiante, Fabio P. Gomes, Suzanne Ostrand-Rosenberg, Catherine Fenselau
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Microbiology
Antonio Edson R. Oliveira, Milton C. A. Pereira, Ashton T. Belew, Ludmila R. P. Ferreira, Larissa M. N. Pereira, Eula G. A. Neves, Maria Carmo P. Nunes, Barbara A. Burleigh, Walderez O. Dutra, Najib M. El-Sayed, Ricardo T. Gazzinelli, Santuza M. R. Teixeira
Article
Infectious Diseases
Michael Smith, Swara H. Yadav, Olayemi Fagunloye, Nana Adjoa J. Pels, Daniel M. Horton, Nashwah M. Alsultan, Andrea Borns, Carolyn T. Cousin, Freddie H. Dixon, Victoria T. Mann, Clarence H. Lee, Paul T. Brindley, Najib H. El-Sayed, Joanna T. Bridger, Matty H. Knight
Summary: The goal to reduce schistosomiasis by controlling the snail population faces challenges due to the complex life cycle of the parasite and lack of vaccines and new drugs. The study highlights the importance of understanding the molecular basis of how schistosomes survive in snails to effectively break the parasite's life cycle.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Immunology
Maria Adelaida Gomez, Ashton Trey Belew, Adriana Navas, Mariana Rosales-Chilama, Julieth Murillo, Laura A. L. Dillon, Theresa A. Alexander, Alvaro Martinez-Valencia, Najib M. El-Sayed
Summary: This study investigates the early host determinants of healing and non-healing responses in human cutaneous leishmaniasis caused by Leishmania (Viannia) panamensis. Transcriptomic profiling of peripheral blood mononuclear cells from CL patients showed that the parasite modulates adaptive immune cell functions early on, impacting immune responses beyond innate and phagocytic functions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ruofan Wang, Ashton T. Belew, Vasudevan Achuthan, Najib El Sayed, Jeffrey J. DeStefano
Summary: Recent analyses have shown that certain subtypes of HIV-1 have higher fidelity in low Mg2+ conditions, while some RTs demonstrate higher fidelity at physiological Mg2+ levels. Sequencing methods have revealed the mutation profiles of HIV-1 RT under different Mg2+ concentrations.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Microbiology
Lindsey R. Burcham, Madeline S. Akbari, Norhan Alhajjar, Rebecca A. Keogh, Jana N. Radin, Thomas E. Kehl-Fie, Ashton T. Belew, Najib M. El-Sayed, Kevin S. McIver, Kelly S. Doran
Summary: Group B Streptococcus (GBS) is associated with severe infections in utero and in newborn populations, and our understanding of the factors required for GBS persistence in the female reproductive tract (FRT) remains limited. In this study, we identified several genes necessary for GBS survival and colonization in the FRT using a transposon mutant library. We found that mutations in the mtsA gene, which is involved in manganese transport, were significantly underrepresented in all in vivo samples collected. We also demonstrated that mtsA contributes to GBS survival during metal limitation and oxidative stress. This work reveals the importance of Mn2+ homeostasis in GBS survival in the FRT.
Article
Multidisciplinary Sciences
Solomon A. Mekonnen, Nour El Husseini, Asan Turdiev, Jared A. Carter, Ashton Trey Belew, Najib M. El-Sayed, Vincent T. Lee
Summary: In this study, a murine model of Pseudomonas aeruginosa CAUTI was used to show that asymptomatic bacteriuria and CAUTI are independent events that require different bacterial virulence factors. This finding has important implications for the treatment and intervention of device-associated infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Lindsey R. Burcham, Yoann Le Breton, Jana N. Radin, Brady L. Spencer, Liwen Deng, Aurelia Hiron, Monica R. Ransom, Jessica da C. Mendonca, Ashton T. Belew, Najib M. El-Sayed, Kevin S. McIver, Thomas E. Kehl-Fie, Kelly S. Doran
Article
Microbiology
Vincent T. Lee, Reza Ghodssi, Najib M. El-Sayed, Rena D. Malik, Olga G. Goloubeva, Tracy H. Hazen, David A. Rasko
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Hematology
Kajal Hamidzadeh, Ashton T. Belew, Najib M. El-Sayed, David M. Mosser
Article
Maqsud Hossain, Munirul Alam, Abdul Khaleque, Sohidul Islam, Abdus Sadique, Nayeim Khan, Zahra Halim, Mrinmoy Sarker, Najib M. El-Sayed, Anwar Huq, Gias Uddin Ahsan, Rita R. Colwell
GENOME ANNOUNCEMENTS
(2018)