Article
Multidisciplinary Sciences
Shaogeng Tang, Yonggang Luc, Will M. Skinnere, Mrinmoy Sanyala, Polina V. Lishko, Masahito Ikawa, Peter S. Kim
Summary: A study finds that knockout of Tmem95 gene causes male sterility in mice. Using a model experiment, it is discovered that human TMEM95 can bind to hamster egg membranes, and a potential receptor-binding surface region is revealed. Further experiments show that the specific, receptor-mediated interaction between sperm TMEM95 and eggs plays a crucial role in membrane fusion during fertilization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Dairy & Animal Science
Mei Matsuzaki, Noritaka Hirohashi, Masaoki Tsudzuki, Mohammad Ibrahim Haqani, Teruo Maeda, Shusei Mizushima, Tomohiro Sasanami
Summary: Research has shown that in birds, sperm from the dominant black (DB) strain has a better ability to enter the sperm storage tubules (SST), resulting in higher fertilization success. This may be due to the longer flagellum and midpiece of DB sperm, allowing them to swim faster in a high viscosity medium.
Article
Agriculture, Dairy & Animal Science
Umar Farooq, Irek A. Malecki, Graeme B. Martin, Misbah Mahmood
Summary: The study developed a method to measure sperm mobility (SM) and investigated its relationship with egg fertility. The results showed that adjustments in Accudenz concentration and incubation temperature had an impact on SM and sperm motility, but there was a weak correlation between SM and egg fertility.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Cell Biology
Mirjam I. Binner, Anna Kogan, Karin Panser, Alexander Schleiffer, Victoria E. Deneke, Andrea Pauli
Summary: Fertilization is a crucial process in sexual reproduction, but the molecular mechanisms behind it are still unclear. A study on zebrafish reveals that Spaca6, a conserved protein, is essential for sperm-egg binding and fusion. The research also uncovers a previously unknown dependence of Dcst2 expression on Spaca6 in sperm.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Soledad N. Gonzalez, Valeria Sulzyk, Mariana Weigel Munoz, Patricia S. Cuasnicu
Summary: Studies on the CRISP protein family in mammalian fertilization have provided insights into their essential roles in gamete interaction and male fertility. Knockout experiments with different CRISP genes revealed their involvement in both gamete interaction and subsequent processes such as sperm transport and early embryo development within the female reproductive tract.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ludmila Curci, Guillermo Carvajal, Valeria Sulzyk, Soledad Natalia Gonzalez, Patricia S. Cuasnicu
Summary: Inactivation of the sperm CatSper channel affects sperm fertilizing ability and function, but does not impact in vitro fertilization. Exposure to HC prior to fertilization significantly reduces in vivo fertilization, indicating the potential for developing a non-hormonal contraceptive by blocking CatSper activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yoshitaka Fujihara, Sarah Herberg, Andreas Blaha, Karin Panser, Kiyonori Kobayashi, Tamara Larasati, Maria Novatchkova, Hans-Christian Theussl, Olga Olszanska, Masahito Ikawa, Andrea Pauli
Summary: Fertilization is a crucial process initiating new individual development in sexually reproducing species. SPACA4, a sperm protein, is essential for efficient fertilization in mammals like mice, particularly for interactions with the zona pellucida. Its absence leads to subfertility in male knockout mice, with fertilization rescue observed when the zona pellucida is removed during in vitro experiments.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Agriculture, Dairy & Animal Science
Michele Bernardino de Lima, Manoela Garcia Borgi Lino de Sousa, Anna Raisa Teixeira Minussi, Lizia Cordeiro de Carvalho, Aline Guedes Veras, Euclides Braga Malheiros, Edney Pereira da Silva
Summary: The objective of this study was to determine the ideal arginine intake for egg production in Japanese quail. The study found that the arginine levels in the diet significantly affected the quail's responses, with egg production increasing from 10% to 97% when the arginine level increased from 0.361% to 1.311%. The models estimated the ideal arginine intake for egg output to be 290 mg/quail per day or 1,411%.
Review
Cell Biology
Ingrid Fliniaux, Guillaume Marchand, Caroline Molinaro, Mathieu Decloquement, Alain Martoriati, Matthieu Marin, Jean-Francois Bodart, Anne Harduin-Lepers, Katia Cailliau
Summary: Sialic acids are a group of monosaccharides with unique physicochemical properties that modulate the biological functions of molecules they are associated with. They play a role in various stages of the reproductive process, particularly in the formation and binding of gametes. Sialoglycoproteins form a specific coat on the surface of gametes, and marine species oocytes have specific polysialylated chains. Spermatozoa undergo crucial changes in sialic acid in the female reproductive tract, facilitating migration, survival, and binding with the oocyte.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lizia Cordeiro de Carvalho, Manoela Garcia Borgi Lino de Sousa, Jaqueline Aparecida Pavanini, Tadia Emanuele Stivanin, Nelson Jose Peruzzi, Alan Rodrigo Panosso, Michele Bernardino de Lima, Edney Pereira da Silva
Summary: This study investigated the effects of lysine supply on egg output in Japanese quail breeders, and determined the ideal lysine intake amount. The results showed that lysine levels significantly affected bird responses, and a monomolecular function was used to interpret and calculate lysine intake.
Article
Multidisciplinary Sciences
Yonggang Lu, Kentaro Shimada, Shaogeng Tang, Jingjing Zhang, Yo Ogawa, Taichi Noda, Hiroki Shibuya, Masahito Ikawa
Summary: The knockout of 1700029I15Rik impairs the biosynthesis of acrosomal membrane proteins and leads to subfertility in male mice. 1700029I15Rik interacts with proteins involved in protein processing, glycosylation, and protein trafficking. Its absence destabilizes the non-catalytic subunits of the OST complex, resulting in decreased abundance of acrosomal membrane proteins and impaired sperm-egg fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Plant Sciences
Wei Deng, Yun Ling Xie, Hui Qiao Tian, Xue Yi Zhu
Summary: This article discusses the early changes that occur in the fused egg of higher plants before the fusion of the nuclei of male and female gametes, especially the unknown egg cell proteins responsible for sperm-egg recognition, attachment, and fusion.
CRITICAL REVIEWS IN PLANT SCIENCES
(2023)
Review
Cell Biology
Katherine A. Maniates, Andrew Singson
Summary: Complementary forward and reverse genetic approaches have led to a recent surge in the discovery of fertilization genes in various model systems. The number of genetically validated gamete surface molecules has more than doubled in the past few years, with all sperm fertilization genes encoding transmembrane or secreted molecules. However, the discovery of genes encoding oocyte molecules has lagged behind. This review discusses potential experimental biases and biological reasons that may have hindered the identification of egg fertilization genes, as well as current strategies to overcome these obstacles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Alberto M. Rivera, Willie J. Swanson
Summary: Gene duplication and repeat domain expansions play an important role in the evolution of fertilization proteins, with gene duplication often facilitating the cooption of molecules into fertilization function and repeat domain expansions providing opportunities for the evolution of novel fertilization proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Food Science & Technology
Karolina Wengerska, Anna Czech, Sebastian Knaga, Kamil Drabik, Tomasz Prochniak, Remigiusz Bagrowski, Angelika Gryta, Justyna Batkowska
Summary: The effect of fermented and non-fermented post-extraction rapeseed meal as a feed additive for Japanese quails was investigated. The addition of 10% fermented rapeseed meal had the most beneficial effect on egg quality traits. There were no significant differences found between birds fed with soybean meal and those fed with fermented and non-fermented rapeseed meal in most of the examined parameters.
Article
Agriculture, Dairy & Animal Science
Mei Matsuzaki, Noritaka Hirohashi, Masaoki Tsudzuki, Mohammad Ibrahim Haqani, Teruo Maeda, Shusei Mizushima, Tomohiro Sasanami
Summary: Research has shown that in birds, sperm from the dominant black (DB) strain has a better ability to enter the sperm storage tubules (SST), resulting in higher fertilization success. This may be due to the longer flagellum and midpiece of DB sperm, allowing them to swim faster in a high viscosity medium.
Article
Agriculture, Dairy & Animal Science
Mei Matsuzaki, Noritaka Hirohashi, Shusei Mizushima, Tomohiro Sasanami
Summary: Studies have shown that in birds, spermatozoa are stored in specialized structures called SSTs located in the UVJ of the oviduct, and certain oligosaccharides on the sperm surface can affect the passage of spermatozoa into SSTs.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Andrology
Hoang Xuan Khoi, Kenji Shimizu, Yoshitaka Yoneda, Itaru Minagawa, Yasuyuki Abe, Yasushi Kuwabara, Tomohiro Sasanami, Tetsuya Kohsaka
Summary: The study found that ROS levels in boar sperm increased gradually after 4-5 days of storage, correlating with decreased motility. Higher ROS levels were associated with higher SH levels and lower motility, particularly in September or early autumn.
Review
Agriculture, Dairy & Animal Science
Mei Matsuzaki, Tomohiro Sasanami
Summary: This article summarizes the current understanding of sperm motility regulation in bird reproductive tracts, and describes the signal transduction mechanisms mainly derived from in vitro studies.
JOURNAL OF POULTRY SCIENCE
(2022)
Article
Reproductive Biology
Mst. Habiba Mostari, Md. Mostafizur Rahaman, Mst. Afroza Akhter, Md. Hasan Ali, Tomohiro Sasanami, Toshinobu Tokumoto
Summary: This study demonstrates the transgenerational adverse effects of bisphenol A (BPA) on zebrafish reproduction. Even low doses of BPA can cause negative effects on gonadal activity, fertility, survival rate, and malformation in the offspring. Furthermore, these adverse effects can be transmitted to subsequent generations.
REPRODUCTIVE TOXICOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Yoshinobu Ichikawa, Shusei Mizushima, Noritaka Hirohashi, Tomohiro Sasanami
Summary: In vitro fertilization is widely used in mammalian species, but the success of fertilization varies. This study focused on the difficulties of mimicking the natural fertilization process in vitro and observed the development of quail eggs after in vitro insemination. The researchers found that a higher number of sperm is required for the progression of the calcium signaling pathway, which is crucial for successful egg development.
JOURNAL OF POULTRY SCIENCE
(2023)
Article
Food Science & Technology
Ryo Ezaki, Tetsushi Sakuma, Daisuke Kodama, Ryou Sasahara, Taichi Shiraogawa, Kennosuke Ichikawa, Mei Matsuzaki, Akihiro Handa, Takashi Yamamoto, Hiroyuki Horiuchi
Summary: Recent genome editing technology has made it possible to generate eggs from chickens that do not contain the major egg allergen, ovomucoid (OVM). In this study, the safety of these eggs as food was evaluated and it was found that the eggs did not contain the mature OVM allergen and the gene editing vectors did not integrate into the chicken's genome. These results highlight the importance of safety evaluation and demonstrate that these OVM knockout eggs can help solve the allergy problem in food and vaccines.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Reproductive Biology
Yoshinobu Ichikawa, Mei Matsuzaki, Shusei Mizushima, Tomohiro Sasanami
Summary: During fertilization in avian species, sperm preferentially penetrate the perivitelline membrane covering the germinal disk region where the female nucleus is present. This study found that annexin A6, expressed on the surface of oocytes, may play a role in sperm-egg interaction in the germinal disk region of Japanese quail, ensuring sperm retention on the egg plasma membrane until fertilization takes place.
REPRODUCTION AND FERTILITY
(2022)
Article
Agriculture, Dairy & Animal Science
Norio Kansaku, Shin Wakui, Tomohiro Sasanami, Takeshi Ohkubo
Summary: The increase in PRL mRNA and PRL content in the anterior pituitary gland during late stage of chicken embryogenesis are closely correlated. However, the initiation of PRL mRNA expression and PRL release from the anterior pituitary gland are regulated by different factors.
JOURNAL OF POULTRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Shusei Mizushima, Tomohiro Sasanami, Tamao Ono, Norio Kansaku, Asato Kuroiwa
Summary: The activation of eggs in Japanese quail is driven by two types of calcium increases mediated by IP3Rs and RYR3. IP3R1 and IP3R3 terminate the initial Ca2+ wave, while RYR3 terminates the spiral-like Ca2+ oscillations.
JOURNAL OF POULTRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Ryo Ezaki, Kennosuke Ichikawa, Mei Matsuzaki, Hiroyuki Horiuchi
Summary: This study successfully integrated a fluorescent protein gene into the CVH locus of chicken PGCs using the CRIS-PITCh method. The knock-in PGCs expressed the fluorescent protein and the efficiency of engineering double knock-in cell lines was confirmed.
JOURNAL OF POULTRY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kennosuke Ichikawa, Yuzuha Motoe, Ryo Ezaki, Mei Matsuzaki, Hiroyuki Horiuchi
Summary: By inserting duck RIG-I with the T2A peptide sequence into chickens, a RIG-I-dependent immune response was established without overexpression of RIG-I and disruption of the host genes. This novel strategy demonstrates a positive feedback loop via RIG-I in chickens.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
