Engineering and antibacterial properties of low-density polyethylene films with incorporated epigallocatechin gallate

The antibacterial activity of low-density polyethylene/adhesive resin (10%)/epigallocatechin gallate (0.03, 0.5, 5, and 10%) extrusion cast films were evaluated against Staphylococcus aureus (gram positive) and Pseudomonassp. (gram negative) via direct contact and in solid and liquid culture media. The epigallocatechin gallate concentration in the active films was established per the invitro antibacterial analysis of pure epigallocatechin gallate against S. aureus and Pseudomonassp. The epigallocatechin gallate migration profile and concentration required to inhibit bacterial growth in broth were determined. In addition, the effects of epigallocatechin gallate and adhesive resin on the mechanical, color, and thermal film properties were investigated. The results indicate that pure epigallocatechin gallate inhibited the growth of both bacteria. However, only the films with 10wt% epigallocatechin gallate (with and without adhesive resin) induced morphological changes and inhibited the growth of S. aureus (p<0.05). In addition, the films with 10wt% epigallocatechin gallate (with adhesive resin) induced morphological changes in Pseudomonassp. (p<0.05). The adhesive resin increased the epigallocatechin gallate release rate in the migration profile (p<0.05). The epigallocatechin gallate and adhesive resin modified the film properties (p<0.05). Fourier transform infrared analysis indicated hydrogen bonds between the adhesive resin and epigallocatechin gallate. This study demonstrated that epigallocatechin gallate is a potential antibacterial agent and that adhesive resin provides advantages to active films.