Article
Plant Sciences
Dong Liang, Zhongqiang Qi, Yan Du, Junjie Yu, Mina Yu, Rongsheng Zhang, Huijuan Cao, Xiayan Pan, Junqing Qiao, Tianqiao Song, Youzhou Liu, Zhiyi Chen, Yongfeng Liu
Summary: This study investigated the conserved molecular mechanisms of the interaction between O. sativa and M. oryzae using transcriptome analysis. The researchers identified several genes that were differentially expressed during the interaction and found that some of these genes are involved in rice resistance and pathogenicity of M. oryzae. These findings contribute to our understanding of the molecular basis of rice-M. oryzae interaction.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Junning Ma, Jean-Benoit Morel, Michael Riemann, Peter Nick
Summary: This study dissects the JA-dependent defense signaling in rice for both compatible and incompatible interactions by using JA-deficient mutants cpm2 and hebiba. The results demonstrate the pivotal role of JA in the basal immunity of rice in its resistance to the Rice Blast Fungus in both types of interactions.
Article
Genetics & Heredity
Bai Li, Zhongkai Chen, Huizhen Chen, Chunlei Wang, Liyan Song, Yue Sun, Yicong Cai, Dahu Zhou, Linjuan Ouyang, Changlan Zhu, Haohua He, Xiaosong Peng
Summary: Introduction of different pest and disease resistance genes is crucial for successful cultivation of transgenic rice strains with broad-spectrum resistance. In this study, resistance rice lines with stacked resistance genes were produced and evaluated for their resistance to multiple pathogens. The findings suggest that stacking of rice resistance genes through molecular marker-assisted backcross breeding can confer broad spectrum and multiple resistance in differently genetic backgrounds.
Article
Plant Sciences
Mandeep Kumari, Ritu Kapoor, B. N. Devanna, Swati Varshney, Richa Kamboj, Amit Kumar Rai, T. R. Sharma
Summary: Deployment of single or multiple blast resistance genes in rice plant is an effective method to enhance resistance against blast disease. This study investigates the proteomics of rice plants with R genes Pi54, Pi54rh, and stacked Pi54 + Pi54rh in response to rice blast infection. The analysis reveals differentially expressed proteins related to defense response against Magnaporthe oryzae, which can be used as targets in future rice resistance breeding.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2023)
Review
Microbiology
Jinyi Tan, Haikun Zhao, Josh Li, Yihan Gong, Xin Li
Summary: This article summarizes the key molecular findings on fungal development and pathogenicity mechanisms of Magnaporthe oryzae, focusing on fully characterized genes based on mutant analysis. These genes are involved in various biological processes of this pathogen, such as vegetative growth, conidia development, appressoria formation and penetration, and pathogenicity. In addition, the article also highlights gaps in our current understanding of M. oryzae development and virulence. The hope is that this review will contribute to a comprehensive understanding of M. oryzae and assist in future disease control strategy designs.
Article
Biotechnology & Applied Microbiology
Yanping Tan, Xiaolin Yang, Minghao Pei, Xin Xu, Chuntai Wang, Xinqiong Liu
Summary: The study aimed to investigate the regulation of gene expression in the interaction between rice and Magnaporthe oryzae using gene chip technology. Changes in gene expression at different time points post-inoculation were analyzed, revealing significant differentially expressed genes and identifying new genes related to rice blast. This research may contribute to the discovery of novel resistance genes and improve the understanding of rice blast development mechanisms.
Article
Plant Sciences
Guihua Duan, Chunqin Li, Yanfang Liu, Xiaoqing Ma, Qiong Luo, Jing Yang
Summary: This study utilized UHPLC/Q-TOF-MS to identify altered metabolites in transgenic rice infected with M. oryzae, revealing organic acids, organic oxygen compounds, lipids, and lipid-like molecules. The research showed that carbohydrate and amino acid metabolic pathways were involved in plant defense responses and resistance strengthening. The study also found that the identified metabolites could be utilized as an optimal marker for M.oryzae defense in rice.
Article
Plant Sciences
Huajun Zhu, Hu Zhou, Zuohua Ren, Erming Liu
Summary: Bacillus subtilis JN005 shows growth promotion and biocontrol efficacy against rice blast, improving germination and growth of rice seeds, enhancing defense enzyme activities in rice leaves, and reducing disease incidence by 79% and 76% in curative and preventive treatments, respectively. Additionally, field experiments demonstrate that spraying with JN005 suspension at 1x10(7) cfu/mL can effectively control rice blast and increase rice production yield.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Biochemistry & Molecular Biology
Shang Gao, Yan Hou, Qiwei Huang, Pengzhi Wu, Zhikai Han, Danhong Wei, Huabin Xie, Fengwei Gu, Chun Chen, Jiafeng Wang
Summary: In this study, the important role of Osa-miR11117 in regulating rice defense mechanisms was investigated. It was found that Osa-miR11117 targets OsPAO4 to regulate blast resistance by adjusting polyamine metabolism and ethylene signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jessie Fernandez, Victor Lopez, Lisa Kinch, Mariel A. Pfeifer, Hillery Gray, Nalleli Garcia, Nick Grishin, Chang-Hyun Khang, Kim Orth
Summary: The study identified two metalloproteinase enzymes, MoMca1 and MoMca2, in M. oryzae, which play significant roles in fungal growth and development, potentially regulating stress responses. The absence of these enzymes resulted in delays in fungal morphogenesis and insights into the mechanisms of M. oryzae pathogenicity.
Article
Plant Sciences
Elgaly K. Salman, Elsayedalaa S. Badr, Kamal E. Ghoniem, Aziza A. Aboulila, Amero A. Emeran
Summary: The study demonstrated that silymarin enhances resistance against rice blast disease in rice, particularly at a concentration of 500 mg/L. Gene expression analysis indicated that silymarin may activate systemic acquired resistance by reinforcing basal plant defense responses.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Zhirong Peng, Ling Li, Shenghai Wu, Xiaolin Chen, Yinfeng Shi, Qiang He, Fu Shu, Wuhan Zhang, Pingyong Sun, Huafeng Deng, Junjie Xing
Summary: This study collected 444 isolates of Magnaporthe oryzae from Hunan Province, China, and characterized the frequencies and genetic variations of AVR genes in the pathogen populations. Pi9 and Pikm showed high resistance to most isolates, while AVRPikm and AVRPi9 were widely distributed with low genetic variation in the pathogen population.
Article
Agronomy
Dagang Tian, Zaijie Chen, Yan Lin, Tingmin Liang, Ziqiang Chen, Xinrui Guo, Feng Wang, Zonghua Wang
Summary: This study investigates the diversity, composition, and assembly of microbial communities associated with the rhizosphere soils and endosphere of Piz-t-KJ201 and NPB-KJ201. The host genotype and inoculation pattern of M.oryzae played dominant roles in determining the microbial community assemblage. Metabolomics analysis revealed that some metabolites may influence changes in bacterial communities.
Article
Biochemistry & Molecular Biology
Xing Dai, Yankai Wang, Kaili Yu, Yonghui Zhao, Langyu Xiong, Ruozhong Wang, Shengben Li
Summary: The overexpression of OsNPR1 enhances rice resistance to Xanthomonas oryzae pv. oryzae (X-oo) by bidirectionally regulating gene expression in rice and X-oo. In X-oo-infected OsNPR1-OE plants, rice genes involved in cell wall biosynthesis, SA signaling pathways, and PR genes are upregulated, while X-oo genes involved in energy metabolism, oxidative phosphorylation, biosynthesis, and transportation are repressed. The downregulation of virulence genes, including components of type III and other secretion systems, is observed in X-oo due to OsNPR1 overexpression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Na Cui, Xiaofeng Chen, Yan Shi, Meirong Chi, Jiantuan Hu, Kunlong Lai, Zonghua Wang, Haifeng Wang
Summary: DNA methylation plays a role in regulating the expression of coding and non-coding regions in plants. A study using whole-genome bisulfite sequencing revealed temporal DNA methylation changes in rice after infection with Magnaporthe oryzae, showing a slight increase in global DNA methylation levels compared to Arabidopsis. Over 38,000 differentially methylated regions (DMRs) were identified, with hypermethylated DMRs dominating over hypomethylated DMRs, most of which were located in transposable element regions. Transcription of NBS-LRR family genes was affected by changes in DNA methylation, indicating the essential role of DNA methylation in rice response to M. oryzae infection.
Article
Agronomy
Jae-Sung Lee, Dmytro Chebotarov, John Damien Platten, Kenneth McNally, Ajay Kohli
Correction
Agriculture, Multidisciplinary
Ely Oliveira-Garcia, Andreas von Tiedemann, Holger B. Deising
Summary: The first author's name was incorrectly published in the original article.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Agriculture, Multidisciplinary
Ely Oliceira-Garcia, Andreas von Tiedemann, Holger B. Deising
Summary: Efficient plant protection is essential for food security, requiring a coordinated approach that includes modern synthetic chemistries among other measures to protect plants from harmful organisms.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Plant Sciences
Kerstin Hoech, Birger Koopmann, Andreas von Tiedemann
Summary: This study investigated the role of lignin in plant defense against Sclerotinia stem rot in oilseed rape. It found that a moderately resistant cultivar showed quicker and more effective defense activation through upregulation of lignin synthesis genes compared to a susceptible cultivar. The results highlight the importance of cell wall modification in quantitative resistance to stem rot by generating distinct lignin types in response to the pathogen.
Article
Plant Sciences
Barbara Ludwig Navarro, Hendrik Hanekamp, Birger Koopmann, Andreas von Tiedemann
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Microbiology
Nazanin Zamani-Noor, Imke Krohne, Birger Koopmann
Summary: The resistance of oilseed rape (OSR) cultivars to clubroot is mainly dependent on a major resistance gene from the cv. Mendel. The greenhouse experiments showed significant differences in resistance parameters among different cultivars, with the threshold of inoculum density for disease occurrence strongly influenced by the virulence of the pathogen and susceptibility of the host plant.
Article
Biochemistry & Molecular Biology
Hedia Tnani, Dmytro Chebotarov, Ranjita Thapa, John Carlos I. Ignacio, Walter K. Israel, Fergie A. Quilloy, Shalabh Dixit, Endang M. Septiningsih, Tobias Kretzschmar
Summary: A novel approach combining haplotype genome-wide association study successfully narrowed down the AG2 gene region and identified 27 candidate genes potentially controlling AG2, including the Rc gene responsible for red pericarp development. Transcriptome analysis revealed differential regulation of sugar metabolism, cell wall modification, and chloroplast development to be implicated in AG tolerance mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Rabia Faridi, Birger Koopman, Antje Schierholt, Mohamed B. Ali, Stefanie Apel, Wolfgang Link
Summary: This study used genome-wide association study to identify markers associated with Ascochyta blight resistance in winter faba beans, finding one marker on chromosome III co-localizes with a known resistance QTL. These new markers have the potential to improve resistance breeding in winter faba beans and support broader inclusion of the crop in rotations.
Article
Microbiology
Lucia Ramos Romero, Dagmar Tacke, Birger Koopmann, Andreas von Tiedemann
Summary: The cultivation area of maize in Central Europe has increased in the last decade due to its high yield potential and diverse uses. However, the more intense cultivation has led to an increase in maize leaf diseases. A study conducted in 2012-2013 identified a novel Phoma-like species infecting maize leaves in Central Europe, indicating the need for further research on this disease.
Article
Plant Sciences
Jae-Sung Lee, Dmytro Chebotarov, Kenneth L. McNally, Valerien Pede, Tri Deri Setiyono, Rency Raquid, Woong-Jo Hyun, Ji-Ung Jeung, Ajay Kohli, Youngjun Mo
Summary: Pre-harvest sprouting (PHS) poses a serious threat to rice production, especially in regions cultivating japonica varieties like California, Japan, and Korea. Promising rice germplasm with strong resistance to PHS has been identified from a diverse rice panel at the International Rice Genebank. A genome-wide association analysis revealed two major loci on chromosomes 1 and 4 associated with PHS resistance in certain japonica accessions. Gene networks suggest a key resistance mechanism related to abscisic acid (ABA), gibberellic acid (GA), and auxin signaling pathways.
Article
Microbiology
Yao Wang, Birger Koopmann, Andreas von Tiedemann
Summary: This study evaluates several methods for assessing the viability and germination rate of Plasmodiophora brassicae resting spores and confirms the suitability of various microscopic and molecular genetic methods. These methods are crucial for studying factors regulating survival, dormancy, and germination of P. brassicae in the soil, and for developing novel control strategies.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Paul Vollrath, Harmeet S. Chawla, Dima Alnajar, Iulian Gabur, HueyTyng Lee, Sven Weber, Lennard Ehrig, Birger Koopmann, Rod J. Snowdon, Christian Obermeier
Summary: Blackleg is a major fungal disease in oilseed rape worldwide, with most commercial cultivars carrying R gene-mediated qualitative resistances that can be rapidly overcome by pathogen mutations. This study identified genomic regions contributing to quantitative resistance in oilseed rape, highlighting the potential for enhancing durability by targeting quantitative resistance in addition to qualitative resistance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Dima Alnajar, Andreas von Tiedemann, Birger Koopmann
Summary: This study reports the Avr profiles and mating type ratios of L. maculans populations in Northern and Central regions of Germany. Different races were identified, with some major resistance genes still providing relatively effective resistance, while others have become ineffective. Sexual reproduction and ascospores play a central role in the evolution of L. maculans. This study provides important data for the development of a strategic plan to preserve the efficacy of major resistance genes in Germany.
Article
Agronomy
Essia Maghrebi, Olfa Beldi, Tahani Ochi, Birger Koopmann, Hanene Chaabane, Bochra Amina Bahri
Summary: Blackleg has been detected in oilseed rape in Tunisia since 2017. Through morphological observations, pathogenicity tests, and sequencing, Leptosphaeria maculans and Leptosphaeria biglobosa were identified as the causes of blackleg. This study provides the first record of L. maculans and L. biglobosa in Tunisia's oilseed rape.
PHYTOPATHOLOGIA MEDITERRANEA
(2023)
Article
Plant Sciences
Rency Raquid, Ajay Kohli, Russell Reinke, Maribel Dionisio-Sese, Jieun Kwak, Dmytro Chebotarov, Youngjun Mo, Jae-Sung Lee
Summary: Seed longevity is crucial for maintaining seed viability and vigor under changing climates, with previous studies focusing on QTL for temperate japonica rice but not tropical japonica rice. Through QTL mapping and candidate gene identification, this study highlights potential mechanisms for enhancing seed longevity in tropical japonica rice varieties.
CURRENT PLANT BIOLOGY
(2021)