Article
Immunology
Chunyu Liu, Chuanfei Yu, Yalan Yang, Yongfei Cui, Feng Zhang, Lan Wang, Junzhi Wang
Summary: The study developed and validated a reporter gene assay to determine the bioactivity of anti-CTLA-4 mAbs, addressing the challenge faced by quality control labs. The assay demonstrated excellent specificity, accuracy, precision, linearity, and cell passage stability, making it suitable for characterization, batch release control, stability study, and biosimilar assessment of anti-CTLA-4 mAbs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Xiao Guo, Chuanfei Yu, Lan Wang, Feng Zhang, Kaiqin Wang, Jing Huang, Junzhi Wang
Summary: A reporter gene assay (RGA) was developed in this study for bioactivity determination of CGRP antibodies, demonstrating good specificity, accuracy, and precision in accordance with ICH-Q2 guidelines. This method can be utilized for quality control and clinical application of therapeutic antibodies targeting CGRP.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Meng Li, Lan Wang, Chuanfei Yu, Junzhi Wang
Summary: The study established a reporter gene assay system based on the OX40-NF kappa B pathway to determine the bioactivity of anti-OX40 monoclonal antibodies. By crosslinking the Fc of the antibodies with OX40, the NF kappa B pathway was activated to evaluate the bioactivity of the antibodies.
Article
Chemistry, Analytical
Xiaojuan Yu, Chuanfei Yu, Kaiqin Wang, Chunyu Liu, Lan Wang, Junzhi Wang
Summary: Type 2 inflammatory cytokines play a significant role in the pathogenesis of asthma, and anti-IL-4R monoclonal antibody has been approved for therapeutic treatment. Researchers developed a bioactivity determination method based on RGA, which demonstrated excellent performance characteristics for anti-IL-4R mAbs through careful optimization of parameters.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Immunology
Chunyu Liu, Chuanfei Yu, Yalan Yang, Jing Huang, Xiaojuan Yu, Maoqin Duan, Lang Wang, Junzhi Wang
Summary: In this study, an engineered monoclonal Jurkat/NFAT/CD32a-Fc epsilon RI gamma effector cell line was developed to measure the bioactivity of antibody-dependent cellular phagocytosis (ADCP). The bioassay was optimized using a Design of Experiment (DoE) strategy and fully validated according to ICH-Q2(R1) to demonstrate good performance characteristics. The established bioassay can be applied to evaluate the ADCP bioactivity for anti-CD20 mAbs and potentially for mAbs targeting other antigens.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Maryam Nakhjavani, Sarah Shigdar
Summary: Triple-negative breast cancer (TNBC) has the worst prognosis among breast cancer subtypes and lacks targeted therapy. Immunotherapy targeting PD-1/PD-L1 has shown promise. Aptamers, with their better tissue penetration and lower production cost compared to mAbs, present a potential pathway for PD-1/PD-L1 modulation in the future.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Changsheng Huang, Shengxiang Ren, Yaqi Chen, Anyi Liu, Qi Wu, Tao Jiang, Panjing Lv, Da Song, Fuqing Hu, Jingqing Lan, Li Sun, Xue Zheng, Xuelai Luo, Qian Chu, Keyi Jia, Yan Li, Jun Wang, Caicun Zou, Junbo Hu, Guihua Wang
Summary: This research discovered that PD-L1 K162 was methylated by SETD7 and demethylated by LSD2. Additionally, PD-L1 K162 methylation controlled the PD1/PD-L1 interaction and significantly enhanced the suppression of T cell activity in controlling cancer immune surveillance. The study demonstrated that PD-L1 hypermethylation was the key mechanism for anti-PD-L1 therapy resistance, identified PD-L1 K162 methylation as a negative predictive marker for anti-PD-1 treatment in patients with non-small cell lung cancer, and showed that the PD-L1 K162 methylation:PD-L1 ratio was a more accurate biomarker for predicting anti-PD-(L)1 therapy sensitivity.
Article
Chemistry, Medicinal
Philippe Bertrand
Summary: Aptamers have emerged as potential alternatives to antibodies or small molecules for targeting immune check points such as PD-1 and PD-L1. They possess high affinity for a wide range of targets including small molecules, proteins, and cells. The SELEX method is used to identify and modify aptamers for specific purposes, and their applications extend to therapy and alternative assay technologies. Strategies for managing aptamer plasma stability can be employed. This perspective provides an overview of the development of aptamers targeting immune checkpoint modulators and other immuno-related targets.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Hikaru Nanamori, Yu Sawada
Summary: Malignant melanoma is a skin cancer with unfavorable clinical behavior, and immunotherapy is currently used for treatment. However, not all patients can benefit from this therapy. To overcome this limitation, clinicians have focused on epigenetic modification, which may have additional therapeutic efficacy by regulating gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Hui Tang, Ruixuan Geng, Xiuxiu Xu, Yingyi Wang, Jiaxin Zhou, Shulan Zhang, Lin Zhao, Mei Guan, Chunmei Bai
Summary: The study found that patients with preexisting ANA and anti-Ro52 antibodies did not have an increased risk of immune-related adverse events when receiving PD-1/PD-L1 inhibitors therapy, but patients positive for antithyroid antibodies should closely monitor immune-related thyroid dysfunction.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Pierre -Florent Petit, Raphaele Bombart, Pierre -Hubert Desimpel, Stefan Naulaerts, Laurie Thouvenel, Jean-Francois Collet, Benoit J. Eynde, Jingjing Zhu
Summary: The delivery of anti-PD-L1 nanobody through tumor-targeting T cells shows promise in overcoming the limitations of immunotherapy based on PD-L1-specific antibodies.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Mark Stroh, Michelle Green, Bjorn L. Millard, Joshua F. Apgar, John M. Burke, Will Garner, Hong Lu, Susan K. Lyman, Luc R. Desnoyers, Jennifer Richardson, Alison Hannah, W. Michael Kavanaugh
Summary: CX-072 is an anti-PD-L1 Probody therapeutic designed to be preferentially activated by proteases in the tumor microenvironment. Model-guided drug development showed that the in vivo activity of CX-072 is consistent with theoretical predictions.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Materials Science, Biomaterials
Tian Gao, Zheng Mao, Wenjing Li, Renjun Pei
Summary: Tumor immune evasion, mediated by the PD-L1/PD-1 axis, suppresses T cell function and enables cancer cells to escape immune surveillance. The aptamer PL1 specifically binds to PD-L1, inhibiting the PD-1/PD-L1 axis and restoring T cell proliferation and function. The similar efficacy and binding capacity of aptamer PL1 as an antibody suggest its potential as an alternative therapeutic agent for cancer immunotherapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Review
Pharmacology & Pharmacy
Vishakha Singh, Amit Khurana, Prince Allawadhi, Anil Kumar Banothu, Kala Kumar Bharani, Ralf Weiskirchen
Summary: The PD-1/PD-L1 axis plays a crucial role in inhibiting immune responses and promoting apoptosis, showing promise in the treatment of chronic liver diseases such as hepatitis and liver cancer. Ongoing research aims to explore the potential of PD-1/PD-L1 inhibitors in these conditions and address associated toxicities.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Rui-na Wang, Qian Yu, Xiao-bo Wang, Di Zhu, Guo-long Li, Zeng-xia Li, Wei Jiang, Wei Li, Yong-jun Dang
Summary: Checkpoint inhibitors, such as PD-1/PD-L1 antibody therapeutics, offer a promising treatment option for multiple cancers. However, the limitations of antibodies have led to the development of small-molecule inhibitors for the PD-1/PD-L1 signaling pathway. Through screening a library of 4169 compounds, including natural products and FDA approved drugs, we discovered that cisplatin and bis(benzonitrile) dichloroplatinum (II) could disrupt PD-1/PD-L1 interaction and inhibit tumor growth in mice with colorectal cancer xenografts.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)