Article
Food Science & Technology
Shuaishuai Chen, Weiyu Guo, Huan Liu, Jiang Zheng, Dingyan Lu, Jia Sun, Chun Li, Chunhua Liu, Yonglin Wang, Yong Huang, Wen Liu, Yongjun Li, Ting Liu
Summary: Psoralen and isopsoralen are major components responsible for Psoraleae Fructus-induced hepatotoxicity. This study reveals that their cytotoxic effects are mediated by metabolic activation through cytochrome P450 (CYP) enzymes. Specific CYP enzymes, such as CYP2C9, 2C19, 2D6, and 2E1, play a crucial role in the metabolic activation of psoralen and isopsoralen. The activation of these compounds by CYP enzymes regulates core targets and signaling pathways, resulting in cytotoxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Plant Sciences
Cai Zhang, Jin-Quan Zhao, Jia-Xing Sun, Hui-Jun Li
Summary: The study revealed that the hepatotoxicity induced by psoralen and isopsoralen is associated with the expression and activity of CYP1A2, possibly through the mechanism of AhR-mediated transcriptional induction of CYP1A2. These findings are important to alert about the potential risks of combined usage of psoralen or isopsoralen with AhR ligands or CYP1A2 substrates in clinical practice.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Chemistry, Medicinal
Cai Zhang, Song Fan, Jin-Quan Zhao, Yan Jiang, Jia-Xing Sun, Hui-Jun Li
Summary: The purpose of this study was to investigate the mechanism underlying psoralen/isopsoralen-induced hepatotoxicity. Through transcriptomic and metabolomic analyses, xenobiotic metabolism via cytochromes P450 was identified as a potential pathway. Further validation identified CYP1A2 as the key metabolic enzyme. Induction of CYP1A2 aggravated hepatotoxicity, while inhibition alleviated the toxic effects. Glutathione adducts with reactive intermediates of psoralen/isopsoralen were detected, suggesting that CYP1A2-mediated metabolic activation is responsible for the observed hepatotoxicity.
PHYTOTHERAPY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Cindy B. McReynolds, Jun Yang, Alonso Guedes, Christophe Morisseau, Roberto Garcia, Heather Knych, Caitlin Tearney, Briana Hamamoto, Sung Hee Hwang, Karen Wagner, Bruce D. Hammock
Summary: This study examined the pharmacokinetics of a novel soluble epoxide hydrolase inhibitor, EC1728, in different animal species for the treatment of pain. Results showed varying drug exposures across species, emphasizing the importance of investigating therapeutic exposures in target species to ensure efficacy and avoid toxicity.
Article
Plant Sciences
Wei-jie Men, Zhao-jun Meng, Qin Wang, Meng-ying Chen, Yu-xia Zhai, Hong Shi, An-hong Wang, Kun Zhou
Summary: This study found that low-dose isopsoralen caused hepatotoxicity after short-term exposure by inhibiting the expression of transporters and amino acid binding enzymes, as well as disrupting bile acid spectrum.
PHARMACEUTICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nhien Nguyen, Christophe Morisseau, Dongyang Li, Jun Yang, Eileen Lam, D. Blake Woodside, Bruce D. Hammock, Pei-an Betty Shih
Summary: This study investigated the expression and activity of soluble epoxide hydrolase (sEH) in healthy women and women with anorexia nervosa. The results showed a correlation between sEH and body mass index, as well as a complex relationship with age and anxiety.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
William P. Watson, Tony Munter, Bernard T. Golding
Summary: Vitamin B12 acts as a partner for enzymatic reactions in humans and its deficiency may increase the risk of neurological illnesses, heart disease and cancer. This study investigated the effect of vitamin B12 on the genotoxicity caused by styrene oxide, a metabolite of styrene. The results suggest that vitamin B12 can protect DNA from genotoxic effects, potentially reducing the risk of carcinogenesis.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Ophthalmology
Meghashri Saravanan, Rong Xu, Olivia Roby, Yekai Wang, Siyan Zhu, Amy Lu, Jianhai Du
Summary: This study investigates the influence of sex on metabolism in the retina, RPE, lens, and brain under fed and fasted conditions. Sex differences in metabolites were observed in different tissues, with the lens and retina showing the most tissue-specific changes. The female RPE and brain were more sensitive to fasting, with decreased metabolites in amino acid metabolism, tricarboxylic acid cycles, and glycolysis. The plasma had the fewest sex-different metabolites, with limited overlap with tissue changes.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Zhen Ye, Kumar Ganesan, Mingquan Wu, Yu Hu, Yingqi She, Qianqian Tian, Qiaobo Ye, Jianping Chen
Summary: The comorbidity of breast cancer and depression is still not well understood, but epoxide metabolism is believed to play a regulatory role and contribute to the construction of the breast cancer microenvironment.
Article
Chemistry, Medicinal
Hao Li, Changyuan Wang, Yue Jin, Yuanqing Cai, Jialin Yao, Qiang Meng, Jingjing Wu, Huihan Wang, Huijun Sun, Mozhen Liu
Summary: Isopsoralen, derived from Psoralea corylifolia, has been found to have anti-postmenopausal osteoporosis effects. It targets multiple proteins and exhibits antioxidative and anti-apoptotic effects.
PHYTOTHERAPY RESEARCH
(2023)
Article
Chemistry, Organic
Jun-ichi Mihara, Kenzo Fujimoto
Summary: K-MEP is a novel photocrosslinker that can be induced to crosslink using visible light and selectively targets thymine bases in a complementary nucleic acid strand. By introducing a methyl group, K-MEP is synthesized more efficiently than the previously reported X-PC, resulting in a higher yield.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
James Raeside, Heather L. Christie
Summary: The examination of conjugates in media from incubations of estrogens in stallion reproductive tract tissues has led to the discovery of a new unidentified metabolite of estrone. This finding suggests a different hydrolysis for desulfation and reveals an additional oxygen atom in these metabolites. These findings may have special interest as possible alternatives to cancer-associated catechol estrogens.
JOURNAL OF ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tae Won Lee, Eunjin Bae, Jin Hyun Kim, Myeong Hee Jung, Dong Jun Park
Summary: This study aimed to evaluate the effects of psoralen (PS), a major active component extracted from Psoralea corylifolia L. seed, on the development and progression of renal fibrosis induced by unilateral ureteral obstruction (UUO). The results showed that PS treatment reduced tubular injury, interstitial fibrosis, and the expression of related molecules. Therefore, PS may be a potential therapeutic agent in the management of chronic kidney disease (CKD).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Mechanics
Binbin Pei, Bofeng Bai
Summary: The mixing of miscible fluids with different densities in turbulent mixing layers is important in chemical engineering. A direct numerical simulation is conducted to investigate the effect of density ratio R between two fluids on the turbulent mixing of species. The results show that the turbulence decays more rapidly in the high-density stream due to the decrease in shear rate and the increase in stratified instability. The study also reveals the dynamical connections between stably stratified mixing layers and wall-bounded turbulent flows.
Review
Biochemistry & Molecular Biology
Baljit Kaur, Palwinder Singh
Summary: This study compiles information about the application of the epoxide functional group in medicinal compounds and biochemical probes, and explores the related advantages and challenges. It also analyzes the strategies that have been adopted for the successful development of epoxide-based compounds within drug discovery programs.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)