Article
Chemistry, Analytical
Haijiao Gao, Guyue Cheng, Hui Wang, Ting Chen, Chao Xu, Houquan Lv, Heying Zhang, Ren Hou, Yulian Wang, Dapeng Peng, Zhonghui Yuan
Summary: A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for rapidly screening androgens in various animal tissues. The method showed good correlation with HPLC-MS/MS results, demonstrating its effectiveness and accuracy for detecting androgens in animal tissues.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Chemistry, Applied
Lulu Zhou, Guifen Chen, Min Chen, Xinying Lu, Yijie Xi, Yun Zhi
Summary: An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using a highly sensitive and specific monoclonal antibody against avilamycin was developed. The optimized ic-ELISA showed low detection limits and good correlation and reliability compared to HPLC.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Applied
Zile Wang, Pimiao Zheng, Jianyi Wang, Shuang He, Zhenhui Ren, Yanfang Zhang, Jincheng Xiong, Haiyang Jiang
Summary: An ic-ELISA method based on a broad-spectrum monoclonal antibody was developed for rapid screening of tropane alkaloids in pig urine, pork, and cereal flour samples. The method showed good sensitivity and reliability, with results correlating well with high performance liquid chromatography-tandem mass spectrometry.
Article
Food Science & Technology
Yanan Wang, Xiaofei Wang, Shuyun Wang, Hanna Fotina, Ziliang Wang
Summary: This study established a highly sensitive and specific ZEN monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the detection of ZEN residues in food and feed. The icELISA showed high sensitivity, specificity, rapidity, and reliability in detecting ZEN, making it a valuable tool for ZEN detection.
Article
Food Science & Technology
Jiao Li, Yuan Ding, He Chen, Wanlin Sun, Yue Huang, Fengquan Liu, Minghua Wang, Xiude Hua
Summary: Propiconazole, a commonly used fungicide in agricultural production, presents health risks. An ic-ELISA method using monoclonal antibodies was developed for accurate detection of propiconazole residues, showing high sensitivity and reliability. The results were consistent with gas chromatography-mass spectrometry analysis, providing an alternative tool for propiconazole determination.
Article
Chemistry, Analytical
Shuangmin Wu, Wei Shen, Yushuang Chen, Yongli Zhu, Ren Hou, Menghong Dai, Dapeng Peng
Summary: In this study, a specific anti-cephalexin monoclonal antibody was used to develop an ic-ELISA method for the determination of cephalexin in animal-derived foods. The established method showed high sensitivity and accuracy, and correlated well with HPLC-MS/MS analysis.
ANALYTICAL LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Yong Xie, Yarong Wang, Xueling Yan, Lu Gan, Tao Le
Summary: The developed ic-ELISA and FLISA are capable of effectively detecting AMOZ residues in animal tissues, showing good recovery rates and correlation coefficients in both spiked experiments and standard LC-MS/MS confirmation tests. These assays are reliable and cost-effective tools for high-throughput monitoring of AMOZ residues.
Article
Infectious Diseases
Sumed Yadoung, Ryoichi Ishimatsu, Zhen-Lin Xu, Korawan Sringarm, Supansa Pata, Marninphan Thongkham, Somporn Chantara, Mookda Pattarawarapan, Surat Hongsibsong
Summary: This study developed an IgY-based icELISA method for detecting antibiotic residues in food samples. The method is fast, easy, sensitive, and cost-effective, with great potential for application.
Article
Chemistry, Applied
Peng Dai, Yan Zhang, Yanping Hong, Jianhua Xiong, Huaying Du, Luying Duan, Dan Wang, Yubo Wang, Weijie Deng, Bruce D. Hammock, Wuying Yang
Summary: A sensitive and reliable icCLEIA method was developed to detect gentamicin residues in animal tissues. By preparing an anti-gentamicin monoclonal antibody and utilizing immunological analysis techniques, gentamicin can be detected rapidly and accurately.
Article
Chemistry, Medicinal
Akihiro Ochi, Shunsuke Fujii, Tomoe Ohta, Takuhiro Uto, Poomraphie Nuntawong, Hiroyuki Tanaka, Satoshi Morimoto, Seiichi Sakamoto
Summary: In this study, a new icELISA method based on a highly specific anti-SSb2 monoclonal antibody was developed for the detection of SSb2 in Kampo medicines. The assay showed high specificity, accuracy, and precision, and the results were strongly correlated with those obtained using liquid chromatograph-mass spectrometer. This suggests that the developed method can be used for the quality control and standardization of Kampo medicines containing Bupleuri radix.
JOURNAL OF NATURAL MEDICINES
(2023)
Article
Food Science & Technology
Xianshu Fu, Erjing Chen, Biao Ma, Ying Xu, Peiying Hao, Mingzhou Zhang, Zihong Ye, Xiaoping Yu, Chaofeng Li, Qingge Ji
Summary: Heavy metals in food packaging materials, especially cadmium, are a global environmental threat. This study proposed an ic-ELISA method based on a specific monoclonal antibody for rapid detection of Cd. The ic-ELISA showed high specificity and accuracy in diagnosing Cd in food packaging materials.
Article
Biochemistry & Molecular Biology
Yuan-yuan Yang, Yu Wang, Yi-feng Zhang, Feng Wang, Yi-fan Liang, Jin-yi Yang, Zhen-lin Xu, Yu-dong Shen, Hong Wang
Summary: This study successfully selected three specific nanobodies against 19-NT and developed an ic-ELISA method using Nb2F7 with low detection limit and excellent recovery rates. The technology showed high correlation with the UPLC-MS/MS method.
Article
Biochemical Research Methods
Poomraphie Nuntawong, Taiki Horikawa, Akihiro Ochi, Shinji Wada, Yumi Tsuneura, Hiroyuki Tanaka, Seiichi Sakamoto, Satoshi Morimoto
Summary: The study aimed to produce an anti-SM monoclonal antibody (MAb) and develop an indirect competitive enzyme-linked immunosorbent assay (icELISA) for standardisation and quality control of Swertia japonica. The developed icELISA was sensitive, with a quantitative range of 0.78 to 12.5 μg/mL, and showed good repeatability with coefficients of variation below 10%. The method was accurate, as confirmed by recoveries in a spike and recovery assay ranging from 91.84% to 115.50%, and the SM content measured using the icELISA was consistent with the results of a high-performance liquid chromatography-ultraviolet (HPLC-UV) assay.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)