Article
Biotechnology & Applied Microbiology
Shiho Yoshida, Alexander M. Kreger, George K. Gittes
Summary: We propose that intra-amniotic sildenafil administration is an effective prenatal therapy for CDH-induced pulmonary hypertension. Intra-amniotic sildenafil treatment attenuated peripheral vascular muscularization, enhanced pulmonary blood flow, and improved lung architecture in CDH fetuses. Early intervention with intra-amniotic sildenafil treatment may be preferable to accelerate lung development and improve prognosis in CDH cases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Ricards Kaulins, Laura Ramona Rozite, Mara Pilmane, Aigars Petersons
Summary: Congenital diaphragm hernia (CDH) is a congenital disease that occurs during prenatal development with significant morbidity and mortality rates. This study evaluated the expression of various growth factors and markers in CDH pathological tissues, revealing multiple morphopathogenetic pathways at play in CDH pathogenesis.
Article
Engineering, Biomedical
Yan Xie, Linawati Sutrisno, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: Interconnected scaffolds promote the chondrogenic differentiation of stem cells. In this study, collagen scaffolds with interconnected pore structures were fabricated using PLGA sponge templates and were used to culture human bone marrow-derived mesenchymal stem cells (hMSCs). The results showed that the interconnected scaffolds can enhance the chondrogenic differentiation of stem cells and improve the production of cartilaginous matrices and the mechanical properties of the implants after subcutaneous implantation.
BIOMEDICAL MATERIALS
(2022)
Article
Polymer Science
Pablo Sanchez-Cid, Mercedes Jimenez-Rosado, Victor Perez-Puyana, Antonio Guerrero, Alberto Romero
Summary: Tissue engineering research focuses on designing scaffolds with better properties to achieve improved and faster tissue regeneration, thereby enhancing people's quality of life. Collagen, the most abundant protein in animals, is widely used in tissue engineering due to its structure, biocompatibility, ease of modification, and processability, making it an ideal scaffold material.
Article
Biochemistry & Molecular Biology
Gerard Boix-Lemonche, Richard M. Nagymihaly, Essi M. Niemi, Natasha Josifovska, Stian Johansen, Morten C. Moe, Hanne Scholz, Goran Petrovski
Summary: Injury of the cornea is a complex biological process that can be facilitated by mesenchymal stromal cells (MSCs) and tissue equivalents. This study presents a new tissue-engineering strategy for corneal stroma regeneration using cellularized 3D bioprinted hydrogel constructs implanted into cultured porcine corneas. The experiment successfully combines laboratory-based methods with patient-care practice to produce a cell-laden tissue equivalent for corneal implantation.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Polymer Science
Gustavo A. Rico-Llanos, Sara Borrego-Gonzalez, Miguelangel Moncayo-Donoso, Jose Becerra, Rick Visser
Summary: Collagen type I is widely used in bone tissue engineering due to its excellent biocompatibility and osteoconductivity, but it also has drawbacks such as high biodegradability and low mechanical strength. Recent efforts have been focused on designing and producing collagen implants to enhance bone regeneration.
Article
Multidisciplinary Sciences
Kantirat Yaja, Sirinda Aungsuchawan, Suteera Narakornsak, Peraphan Pothacharoen, Rungusa Pantan, Waleephan Tancharoen
Summary: This study compared the effectiveness and safety of different culture supplements (FBS or hPL) on the osteoblastic differentiation potential of hAF-MSCs under a three-dimensional gelatin scaffold. The results showed that hAF-MSCs have the potential to be used in clinical applications and hPL as a supplement significantly stimulated osteoblast differentiation and had better gene expression and cell functionality compared to FBS.
Article
Engineering, Biomedical
Stefania Scialla, Fabiana Gullotta, Daniela Izzo, Barbara Palazzo, Francesca Scalera, Ivan Martin, Alessandro Sannino, Francesca Gervaso
Summary: Articular cartilage degeneration is a challenging issue with limited repair capabilities. Tissue engineering strategies using appropriate scaffolds for chondrogenic differentiation of stem cells can be a promising solution. This study investigated the influence of two genipin crosslinking routes on collagen-based scaffolds and found that bulk crosslinking with genipin resulted in a more uniform and homogeneous deposition of chondral-like matrix.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Materials Science, Multidisciplinary
Chunyang Ma, Hetong Wang, Yongjie Chi, Yanling Wang, Le Jiang, Nan Xu, Qiong Wu, Qingling Feng, Xiaodan Sun
Summary: Collagen fibers in the body have a complex arrangement, which plays an important role in cell adhesion, migration, proliferation, and secretion. Oriented collagen fiber scaffolds have shown exciting results in tissue repair. Bone is crucial for bearing loads in the human body, and the arrangement of collagen fibers in bone significantly affects its mechanical properties and physiological functions. Oriented collagen fiber scaffolds can promote new bone growth and induce cells to secrete oriented extracellular matrix. However, the complex arrangement of collagen fibers in bone poses challenges for bone tissue repair and bionics.
APPLIED MATERIALS TODAY
(2021)
Review
Medicine, General & Internal
Han Luo, Zhen Wang, Fang Qi, Dali Wang
Summary: Complete wound regeneration is crucial for maintaining skin structure and physiological functions, and amniotic fluid stem cells (AFSCs) have the potential to play a significant role in wound generation and treatment. AFSCs are easily accessible and have advantages such as anti-inflammatory and immunomodulatory properties, which can promote full wound regeneration.
CHINESE MEDICAL JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Charles M. Bowen, Frederick S. Ditmars, Ashim Gupta, Jo-Anna Reems, William Samuel Fagg
Summary: Amniotic fluid, specifically its cell-free form, has regenerative properties and can stimulate immunomodulatory and pro-growth characteristics in target cells or tissues. It has been shown to effectively treat cutaneous wounds and orthopedic degenerative conditions.
Review
Biochemistry & Molecular Biology
Flaminia Pugnaloni, Irma Capolupo, Neil Patel, Paola Giliberti, Andrea Dotta, Pietro Bagolan, Florian Kipfmueller
Summary: This review highlights the importance of miRNAs in CDH-related PH and summarizes the findings from animal and human CDH studies. The focus on epigenetic modulators of CDH-PH offers potential for developing innovative diagnostic tools and treatment approaches, and enhances researchers' understanding of CDH pathophysiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Tina Navaei, Peiman Brouki Milan, Ali Samadikuchaksaraei, Hamid Reza Davari, John G. Hardy, Masoud Mozafari
Summary: The porous PCL/gelatin scaffolds designed in this study showed potential for enhancing diaphragmatic reconstruction in both in vitro and in vivo environments, exhibiting superior cellular behavior and attachment. Compared to PCL scaffolds, the gelatin-containing scaffolds promoted better cell attachment and growth.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Engineering, Biomedical
Thomas L. Jenkins, Paula A. Sarmiento Huertas, Kentaro Umemori, Farshid Guilak, Dianne Little
Summary: This study aimed to evaluate crosslinking methods to enhance the retention of tendon-derived matrix (TDM) on electrospun poly(e-caprolactone) (PCL) scaffolds and stimulate tendon-like matrix synthesis. The results showed that crosslinking TDM to electrospun scaffolds improves tendon-like matrix synthesis, providing a viable strategy for improving the retention of TDM on electrospun PCL scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Pharmacology & Pharmacy
Alecsander F. Bressan, Victoria Oliveira Maia, Beatriz de Souza Rodrigues, Giuliana Bertozi, Sabrina S. Batah, Alexandre T. Fabro, Gloria Pelizzo, Ugo Maria Pierucci, Rita C. Tostes, Lourenco Sbragia, Fernando S. Carneiro
Summary: Congenital diaphragmatic hernia (CDH) can cause abnormalities in the pulmonary arteries of both the contralateral and ipsilateral sides of the diaphragm. Nitric oxide (NO) is commonly used to treat CDH but may not always be effective. This study investigated the different responses of the left and right pulmonary arteries to a NO donor in a rabbit model of left CDH. The results showed that newborns with CDH had increased vasorelaxant responses to the NO donor compared to the control group, possibly due to increased cGMP mobilization.
PHARMACOLOGICAL RESEARCH
(2023)