Article
Chemistry, Medicinal
Chen Chen, Geting Ye, Jing Tang, Jialin Li, Wenbin Liu, Li Wu, Yuhua Long
Summary: Four new polyketide compounds and two new citreoviridin derivatives were isolated from the mangrove endophytic fungus Penicillium sp. BJR-P2. Compound 2 exhibited stronger anti-inflammatory activity by inhibiting NO production induced by LPS.
Article
Chemistry, Medicinal
Xuan Lu, Jun-kai Qi, Xiao-yuan Tang, Xu-de Wang, Chong-tao Ye, Jing Bai, Chuan Tang, Yu-fei Xi, Bin Wu, Xue Wan, Bao-min Feng
Summary: Seven undescribed polyketide compounds (1-4, 9-11) and six known polyketide compounds (5-8,12, 13) were isolated from Rhodiola tibetica endophytic Penicillium sp. HJT-A-10. The structures of the seven undescribed polyketide metabolites were determined using spectroscopic methods. The results of the anti-inflammatory activity assay demonstrated that compounds 1-8, 10-13 exhibited significant inhibitory effects on LPS-induced NO production in RAW 264.7 cells.
Article
Biochemistry & Molecular Biology
Jeferson Rodrigo Souza Pina, Joao Victor Silva-Silva, Josiwander Miranda Carvalho, Heriberto Rodrigues Bitencourt, Luciano Almeida Watanabe, Juan Matheus Pereira Fernandes, Guilherme Eduardo de Souza, Anna Caroline Campos Aguiar, Rafael Victorio Carvalho Guido, Fernando Almeida-Souza, Katia da Silva Calabrese, Patricia Santana Barbosa Marinho, Andrey Moacir do Rosario Marinho
Summary: The natural compound ravenelin showed antibacterial and antiparasitic activities, with significant effects on Gram-positive bacteria and Plasmodium falciparum, while exhibiting low cytotoxicity on HepG2 and macrophage cells. Ravenelin demonstrated attractive selectivity for parasites, making it a promising candidate for further development.
Article
Chemistry, Medicinal
Wencong Yang, Qi Tan, Yihao Yin, Yan Chen, Yi Zhang, Jianying Wu, Leyao Gao, Bo Wang, Zhigang She
Summary: Eight new compounds were isolated from the endophytic fungus Talaromyces sp. CY-3, including sambutoxin derivatives, highly oxygenated cyclopentenones, and highly oxygenated cyclohexenones. Some of these compounds exhibited better alpha-glucosidase inhibitory activity compared to the positive control.
Article
Pharmacology & Pharmacy
Qianqian Xu, Yuben Qiao, Zijun Zhang, Yanfang Deng, Tianqi Chen, Li Tao, Qiaoxin Xu, Junjun Liu, Weiguang Sun, Ying Ye, Yuanyuan Lu, Changxing Qi, Yonghui Zhang
Summary: Two new polyketide compounds, asperulosins A and B, and one new prenylated small molecule, asperulosin C, along with nine known compounds were isolated from a fungus Aspergillus rugulosa. The new compounds showed potential as anti-inflammatory agents, inhibiting NO production and modulating cytokine secretion in a manner comparable to the positive control, dexamethasone.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jun Tang, Xueshuang Huang, Ming-Hang Cao, Zhiyan Wang, Zhiyin Yu, Yijun Yan, Jian-Ping Huang, Li Wang, Sheng-Xiong Huang
Summary: New mono-/bis-alkenoic acid derivatives were isolated from an endophytic fungi, and their chemical structures were determined. One of the derivatives exhibited activity against Candida albicans and Exserohilum turcicum.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Applied
Hyeri Ku, Ji-Yun Baek, Ki Sung Kang, Sang Hee Shim
Summary: In this study, a new oxazole-type compound, macrooxazole E, along with three known macrooxazoles A-C were isolated from the ethyl acetate extracts of the endophytic fungus Phoma sp. JS0228. The structures of these compounds were determined using spectroscopic methods. One of the compounds, macrooxazole C, exhibited moderate anti-proliferative activities against human breast cancer and prostate cancer cell lines, indicating the potential of the endophytic fungus for producing bioactive natural compounds.
NATURAL PRODUCT RESEARCH
(2022)
Article
Plant Sciences
Hai-Yan Cao, Cheng Yi, Sen-Feng Sun, Yong Li, Yun-Bao Liu
Summary: Twelve new dimeric tetrahydroxanthones, named muyocoxanthones A-L (1-12), were isolated from the endophytic fungus Muyocopron laterale. The structures and inhibitory activity of these compounds were determined. Compounds 2, 6, and 11 showed inhibitory activity against LPS-induced nitric oxide production.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Hui-Ting Zeng, Yi-Hu Yu, Xi Zeng, Miao-Miao Li, Xia Li, Shan-Shan Xu, Zong-cai Tu, Tao Yuan
Summary: Eight new polyketides, including three benzophenone dimers, were isolated from the fungus Pleosporales sp. YY-4. These dimeric benzophenones showed inhibitory effects on NO production in RAW-264.7 cells.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Bruno Perlatti, Nan Lan, Meichun Xiang, Cody E. Earp, Joseph E. Spraker, Colin J. B. Harvey, Connie B. Nichols, J. Andrew Alspaugh, James B. Gloer, Gerald F. Bills
Summary: Research identified new metabolites from fungal fermentations for inhibiting Cryptococcus neoformans, including a novel 24-carbon macrolide metabolite and a cytotoxin. These compounds showed moderate to potent antifungal activity against the pathogenic yeast C. neoformans.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Yuanyuan Lu, Qianqian Xu, Liangfang Pang, Yarui Liu, Liangxia Li, Jiayan Yan, Yonghui Zhang, Ziming Huang
Summary: In this study, four compounds with antibacterial and lipid-lowering activities were isolated from the fresh Siegesbeckia pubescens Makino. Among them, two new fungal polyketides with unique structures and absolute configurations were discovered.
NATURAL PRODUCT RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
Xiao-Wei Guo, Zhen-Qing Yu, Junmin Xi, Hao Ren, Xin-Yu Xiang, Jia Wu, Jianguo Fang, Quan-Xiang Wu
Summary: Sixteen new polyketides, including four novel carbon skeletons, were isolated from an endophytic fungus. The structures of these polyketides were determined using X-ray diffraction analysis and electric circular dichroism spectra comparison. The polyketides showed low toxicity and exhibited excellent antioxidant activity, with one compound showing greater efficacy than resveratrol in scavenging free radicals. These findings suggest that the novel polyketides could be potential antioxidant agents for neuroprotection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Ran Zhang, Yujia Wang, Guowei Cai, Juxian Wang, Jianyuan Zhao, Jinglin Bai, Tao Zhang, Shan Cen, Wenni He, Liyan Yu
Summary: Six new phthalide derivatives, namely Verbalide A similar to F (1-6), and one known derivative (7), were isolated from the endophytic fungus Preussia sp. CPCC 400972. The structures of these compounds were determined through comprehensive spectroscopic analyses, including NMR and HRESIMS. Furthermore, compounds 1-7 showed excellent inhibitory effect against influenza A virus.
JOURNAL OF ANTIBIOTICS
(2023)
Article
Chemistry, Medicinal
Yan Chen, Ge Zou, Wencong Yang, Yingying Zhao, Qi Tan, Lin Chen, Jinmei Wang, Changyang Ma, Wenyi Kang, Zhigang She
Summary: New compounds were isolated from the endophytic fungus, with two showing potent anti-inflammatory activities. The structures were determined through extensive analysis, and the absolute configurations were established by ECD calculations and specific rotation comparison.
Article
Microbiology
Wenya Weng, Shicui Jiang, Chuchu Sun, Xiaofu Pan, Li Xian, Xuemian Lu, Chi Zhang
Summary: In this study, eight structurally diverse secondary metabolites were isolated and identified from the endophytic fungus Penicillium sp. YT2019-3321 derived from traditional Chinese medicine Lonicera japonica. Among them, a new compound exhibited dose-dependent cytotoxicity against human pancreatic tumor cells and induced apoptosis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Linda Jansen-Olliges, Shambhabi Chatterjee, Lili Jia, Frank Stahl, Christian Baer, Marc Stadler, Frank Surup, Carsten Zeilinger
Summary: Protein microarray screenings have identified fungal natural products from the azaphilone family as powerful inhibitors of SARS-CoV-2 spike protein binding to ACE2 receptors. Among them, Cohaerin F exhibited the highest potency in reducing ACE2 and SARS-CoV-2 spike protein binding by more than 50%. Further analysis led to the discovery of six new multiformins and the revision of the stereochemistry of multiformins. Cell-based infection assays revealed that Cohaerin C and Cohaerin F significantly reduced SARS-CoV-2 pseudotyped lentivirus particles infection, while Multiformin C and G almost completely abolished the infection. These findings highlight the preventive potential of multiformin-type azaphilones against SARS-CoV-2 binding to ACE2 receptors.
Article
Pharmacology & Pharmacy
Katharina Rox, Tim Becker, Andrea Schiefer, Miriam Grosse, Alexandra Ehrens, Rolf Jansen, Tilman Aden, Stefan Kehraus, Gabriele M. Koenig, Anna K. Krome, Marc P. Huebner, Karl G. Wagner, Marc Stadler, Kenneth Pfarr, Achim Hoerauf
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a high priority pathogen organism, causing significant global mortality. The natural product CorA, currently in preclinical development, demonstrates effectiveness against MRSA in vitro. This study evaluates the pharmacokinetics and pharmacodynamics of CorA, suggesting its potential for MRSA and MSSA-related infections.
Article
Microbiology
Sarunyou Wongkanoun, Boonchuai Chainuwong, Noppol Kobmoo, Sittiruk Roytrakul, Sayanh Somrithipol, Jennifer Luangsa-ard, Esteban Charria-Giron, Prasert Srikitikulchai, Marc Stadler
Summary: In this study, hypoxylaceous specimens collected from several sites in Thailand over the past two decades were examined to determine their affinity to the genus Pyrenopolyporus. The researchers used a combination of morphological, metabolomic, and molecular techniques to describe and illustrate five novel species and a new record for the country. Proteomic profiles of the fungi were also provided for the first time, using matrix associated laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF/MS).
Article
Chemistry, Organic
Bel Youssouf G. Mountessou, Elodie Gisele M. Anoumedem, Blondelle M. Kemkuignou, Yasmina Marin-Felix, Frank Surup, Marc Stadler, Simeon F. Kouam
Summary: A new species of Diaporthe cameroonensis was isolated from the fresh root of Trema guineensis (Ulmaceae). The strain was fermented and extracted, resulting in the isolation of a hemiketal polyketide and an acetylated alternariol, as well as fifteen known secondary metabolites.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Mycology
Suchada Mongkolsamrit, Birthe Sandargo, Sherif Saeed Ebada, Wasana Noisripoom, Somruetai Jaiyen, Janet Jennifer Luangsa-ard, Marc Stadler
Summary: Fungal specimens parasitic on spider egg sacs were identified based on molecular phylogenetic analyses and morphological data, and a novel monotypic genus named Bhushaniella rubra was described. The fungus produces superficial perithecia and its anamorph has verticillate phialides with a slightly curved neck. The evaluation of its secondary metabolites revealed the presence of penicolinate type alkaloids, with no significant antibiotic activity or cytotoxic effects.
MYCOLOGICAL PROGRESS
(2023)
Review
Biochemistry & Molecular Biology
Christopher Lambert, Katharina Schmidt, Marius Karger, Marc Stadler, Theresia E. B. Stradal, Klemens Rottner
Summary: The eukaryotic actin cytoskeleton is a dynamic network composed of monomeric and filamentous forms of actin, as well as actin-binding proteins (ABPs). Small molecular inhibitors have been used to study the importance of actin filaments for cellular processes, by interfering with the interactions and turnover of the actin cytoskeleton. This review aims to summarize the available information on the structural features of cytochalasans and their effects on cell morphology and actin cytoskeleton organization.
Article
Chemistry, Multidisciplinary
Kunthida Phutthacharoen, Syeda J. Khalid, Hedda Schrey, Kevin D. Hyde, Marc Stadler, Sherif S. Ebada
Summary: In this study, two polyketide metabolites were identified from the mycelial extract of a saprotrophic fungus growing on common reed grass. One of the compounds exhibited potent cytotoxicity against tested cell lines, while the other showed moderate inhibitory effects on the formation of S. aureus biofilms.
Article
Microbiology
Blondelle Matio Kemkuignou, Christopher Lambert, Marc Stadler, Simeon Kouam Fogue, Yasmina Marin-Felix
Summary: Four unprecedented polyketides were isolated from the newly described endophytic fungus Diaporthe africana. The fungus was taxonomically identified using a polyphasic approach. The structures of the compounds were determined by spectral analysis, and their antimicrobial and cytotoxic properties were evaluated.
Article
Microbiology
Haoxuan Zeng, Marc Stadler, Wolf-Rainer Abraham, Mathias Muesken, Hedda Schrey
Summary: The fungal human pathogens, Candida auris and Candida albicans, have various virulence mechanisms, including the formation of biofilms and the development of (pseudo)hyphae to protect against harsh conditions and facilitate invasion of host tissues. Increasing rates of antifungal resistance in these pathogens pose a challenge for effective treatment. This study found that rubiginosin C, derived from the ascomycete Hypoxylon rubiginosum, effectively inhibited biofilm formation and hyphal development in both C. auris and C. albicans without harming host cells. Rubiginosin C is a promising candidate for therapeutic intervention in the future.
Article
Biotechnology & Applied Microbiology
Winnie Chemutai Sum, Sherif S. Ebada, Josphat Clement Matasyoh, Marc Stadler
Summary: This review summarizes recent reports on biologically active secondary metabolites from Basidiomycota in the past five years. These organisms are still largely unexplored, with a predominance of terpenoids and meroterpenoids, but also commonly encountered polyketides, alkaloids, and other nitrogen-containing metabolites. A significant number of novel compounds have been reported from African and Asian species, while there have been very few new records from American Basidiomycota and only a handful from European mushrooms. In total, over 350 new metabolites have been recorded.
CURRENT RESEARCH IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Nico Mitschke, Winnie Chemutai Sum, Khadija Hassan, Marco Kirchenwitz, Hedda Schrey, Luca Gerhards, Harald Kellner, Theresia E. B. Stradal, Josphat C. Matasyoh, Marc Stadler
Summary: Four new drimane sesquiterpenoids were discovered in the submerged cultures of the basidiomycete Dentipellis fragilis. The compounds were characterized using advanced spectroscopic techniques and their biological activities were evaluated. One compound showed weak antimicrobial activity, while two others exhibited neurotrophic effects. This study provides new insights into the bioactive compounds produced by this group of fungi.
Article
Chemistry, Multidisciplinary
Sarunpron Khruengsai, Patcharee Pripdeevech, Chutima Tanapichatsakul, Winnie Chemutai Sum, Mahmoud A. A. Ibrahim, Marc Stadler, Sherif S. S. Ebada
Summary: In this study, two new metabolites (1 and 2) and three known compounds were isolated from the submerged cultures of L. chiangraiensis. The chemical structures of the isolated compounds were determined using NMR spectroscopic analyses and HRESIMS, and the absolute configurations of the new compounds were established using TDDFT-ECD spectra. Compound 1 showed significant cytotoxic activities against various cell lines and moderate antibacterial effects.
Article
Chemistry, Multidisciplinary
Carsten Peukert, Anna C. Vetter, Hazel L. S. Fuchs, Kirsten Harmrolfs, Bianka Karge, Marc Stadler, Mark Bronstrup
Summary: The study aimed to enhance the potency of existing antibiotics against antibiotic-resistant Gram-negative bacteria. By utilizing the microbial iron transport machinery, covalent modifications were made to improve drug translocation across the cell membrane. Cleavable linkers were designed to release the antibiotic inside the bacteria and unperturbed target binding. The conjugates showed increased antibiotic activity against multidrug-resistant E. coli compared to free rifamycin, suggesting the potential of this approach to boost the effectiveness of existing antibiotics.
Article
Biochemistry & Molecular Biology
Ozge Demir, Haoxuan Zeng, Barbara Schulz, Hedda Schrey, Michael Steinert, Marc Stadler, Frank Surup
Summary: A fungal endophyte from the Canary Islands has been identified as an effective antagonist against the fungal phytopathogen that causes ash dieback disease. The endophyte produces various secondary metabolites, including a new compound called peziculastatin. These compounds show significant inhibitory or dispersive effects on fungal and bacterial biofilms.
Article
Chemistry, Multidisciplinary
Henrike Heinemann, Kevin Becker, Hedda Schrey, Haoxuan Zeng, Marc Stadler, Russell J. Cox
Summary: Submerged cultivation results in the formation of new sulfur-containing sporothriolide derivatives, which show attenuated antimicrobial activity due to the presence of 2-hydroxy-3-mercaptopropanoic acid. However, these derivatives still exhibit moderate effects on the biofilms of Candida albicans and Staphylococcus aureus at lower concentrations.