Hybrid sol-gel inorganic/gelatin porous fibres via solution blow spinning

Hybrid sol-gel inorganic-organic fibres offer great potential in tissue engineering and regenerative medicine. A significant challenge is to process them using scalable technologies into useful scaffolds that provide control over fibre diameter, morphology, mechanical properties, ion release, degradation and cell response. In this work we develop formulations that are amenable to processing via solution blow spinning (SBS), a rapid technique using simple equipment to spray nano-/micro-fibres without any electric fields. The technique is extended to produce porous class I and II hybrid fibres using cryogenic SBS, with formulations developed based on tetraethyl orthosilicate/gelatin that are relatively facile to lyophilise. The formulations developed here take advantage of the reversible thermally activated conformation change of gelatin in aqueous solutions from random coil to triple helix to enable viscosity tuning and therefore fibre spinning. Gelatin is functionalised with (3-glycidyloxypropyl)trimethoxysilane to produce class II hybrids which exhibit controllable time- and temperature-dependent viscosity profiles which can be tuned for spinning into highly porous fibres.