Article
Immunology
Jingfeng Tong, Lu Meng, Cheng Bei, Qingyun Liu, Min Wang, TingTing Yang, Howard E. Takiff, Shuye Zhang, Qian Gao, Chuan Wang, Bo Yan
Summary: The modern Beijing sublineage of Mycobacterium tuberculosis has increased virulence and faster in vitro growth, triggering a strong immune response and macrophage infiltration. Transcriptomic analysis revealed enrichment of infection, cholesterol homeostasis, and amino acid metabolic pathways in infected macrophages. The upregulation of proinflammatory/bactericidal cytokines is consistent with the reduced bacterial burden in macrophages infected with modern strains, suggesting a hyperinflammatory response and stronger virulence.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Immunology
Susana Lopez-Lopez, Maria Jose Romero de Avila, Natalia Carolina Hernandez de Leon, Francisco Ruiz-Marcos, Victoriano Baladron, Maria Luisa Nueda, Jorge Laborda, Jose Javier Garcia-Ramirez, Eva M. Monsalve, Maria Jose M. Diaz-Guerra
Summary: NOTCH4 acts as a negative regulator in macrophage activation by interfering with proinflammatory cytokine expression and signaling pathways such as IFN-gamma and STAT1. It also modulates the balance between STAT1 and STAT3 activation, impacting the transcriptional activity of macrophages. Moreover, NOTCH4 affects NF-kappa B and TLR signaling pathways, revealing new insights into the regulation of macrophage-specific effector functions.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Marietta M. Ravesloot-Chavez, Erik Van Dis, Sarah A. Stanley
Summary: Infection with Mycobacterium tuberculosis causes a significant number of deaths annually worldwide, where the role of innate immune cells and the inflammatory response in the infection process are crucial yet complex.Understanding the balance of cell-intrinsic control and inflammation regulation is essential for developing effective therapeutics and vaccines.
ANNUAL REVIEW OF IMMUNOLOGY, VOL 39
(2021)
Article
Cell Biology
Qinmei Ma, Jialin Yu, Li Liu, Xiaoyan Ma, Jiaxue Zhang, Jiamei Zhang, Xiaoping Wang, Guangcun Deng, Xiaoling Wu
Summary: This study reveals that TRAF6 interacts with lysosomal protein Rab7 and promotes the fusion of autophagosomes and lysosomes by ubiquitinating Rab7 and binding to STX17. Furthermore, TRAF6 enhances the initiation and formation of autophagosomes in Mycobacterium-induced autophagy, while TRAF6 deficiency exacerbates lung injury and promotes the survival of Mycobacterium.
CELL DEATH DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Junghwan Lee, Seong-Ahn Lee, Sang-Hun Son, Ji-Ae Choi, Tam Doan Nguyen, Jaewhan Kim, Doyi Son, Chang-Hwa Song
Summary: This study found that BNIP3 plays a critical role in regulating mitophagy during Mycobacterium tuberculosis (Mtb) infection. Inhibiting mitophagy suppresses Mtb growth in macrophages through increased mitochondrial reactive oxygen species (mROS) production. Therefore, BNIP3 might be a novel therapeutic target for tuberculosis treatment.
CELL AND BIOSCIENCE
(2023)
Article
Immunology
Weifeng Gao, Yurong Cai, Gang Zhang, Xiaoping Wang, Juan Wang, Yong Li, Yujiong Wang
Summary: This study used dual lipidomics to uncover the differences in lipid composition between Mycobacterium tuberculosis and Mycobacterium bovis, as well as the lipid metabolism responses in primary bovine alveolar macrophages infected by these bacteria. The results showed significant differences in various lipid categories between the two species and revealed distinct lipid metabolism responses in host macrophages. The findings suggest that the differential lipid compositions enable the successful colonization and development of the bacteria in macrophages through different mechanisms.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biology
Ekansh Mittal, Andrew T. Roth, Anushree Seth, Srikanth Singamaneni, Wandy Beatty, Jennifer A. Philips, Bavesh D. Kana
Summary: For decades, researchers have studied the interaction between Mycobacterium tuberculosis (Mtb) and macrophages, a major cellular environment for the bacterium. Different methods used to disaggregate Mtb have been found to impact the bacterial cell envelope integrity, macrophage inflammatory responses, and intracellular Mtb survival. Sonication and filtering, two commonly used preparation methods, were found to damage the mycobacterial cell envelope and affect the outcome of infections in mouse macrophages. These findings highlight the potential for experimental artifacts in Mtb-host interaction studies and the interpretation of bacterial mutants due to the widely used disaggregation methods.
Review
Immunology
Haotian Bo, Ulrich Aymard Ekomi Moure, Yuanmiao Yang, Jun Pan, Li Li, Miao Wang, Xiaoxue Ke, Hongjuan Cui
Summary: Mycobacterium tuberculosis (Mtb), the causative agent of TB, interacts with macrophages in the lungs, subverting the host immune system and causing infection. This review examines Mtb effectors that interact with macrophages and how macrophages control and eliminate Mtb, as well as how Mtb manipulates macrophage defense mechanisms for its own survival. Understanding these mechanisms is crucial for TB prevention, diagnosis, and treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
William R. Schwan
Summary: This study demonstrates that the SK-03-92 lead compound can kill Mycobacterium tuberculosis bacteria within mammalian macrophages.
Article
Multidisciplinary Sciences
Celine Souilhol, Blanca Tardajos Ayllon, Xiuying Li, Mannekomba R. Diagbouga, Ziqi Zhou, Lindsay Canham, Hannah Roddie, Daniela Pirri, Emily Chambers, Mark J. Dunning, Mark Ariaans, Jin Li, Yun Fang, Helle F. Jorgensen, Michael Simons, Rob Krams, Johannes Waltenberger, Maria Fragiadaki, Victoria Ridger, Sarah De Val, Sheila E. Francis, Timothy J. A. Chico, Jovana Serbanovic-Canic, Paul C. Evans
Summary: This study demonstrates the role of the JAG1-NOTCH4 signaling pathway in sensing disturbed blood flow and promoting atherosclerosis susceptibility by regulating endothelial cell heterogeneity.
Article
Microbiology
Graciela Teran, Hanxiong Li, Sergiu-Bogdan Catrina, Ruining Liu, Susanna Brighenti, Xiaowei Zheng, Jakob Grunler, Susanne Nylen, Berit Carow, Martin E. Rottenberg
Summary: People with diabetes who are also infected with M. tuberculosis are at a higher risk of developing tuberculosis. The mechanisms behind this increased risk are not well understood. This study shows that the transcription factor HIF-1 plays a crucial role in the outcome of M. tuberculosis infection in macrophages. High glucose and reactive carbonyl compounds inhibit HIF-1 responses, leading to impaired control of intracellular M. tuberculosis. Treatment with a HIF-1 stabilizer restores mycobacterial control in the presence of glucose or carbonyl stress. The findings suggest that targeting HIF-1 may reduce the risk of developing tuberculosis in people with diabetes.
Article
Immunology
Jun Fang, Chunsheng Dong, Sidong Xiong
Summary: This study demonstrates that Rv0790c, a protein encoded by Mycobacterium tuberculosis, promotes bacterial survival by facilitating cellular autophagy at its early stage. This finding provides insights into the mechanisms through which Mycobacterium tuberculosis evades host immune responses and offers potential targets for tuberculosis treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Beibei Fu, Xiaoyuan Lin, Shun Tan, Rui Zhang, Weiwei Xue, Haiwei Zhang, Shanfu Zhang, Qingting Zhao, Yu Wang, Kelly Feldman, Lei Shi, Shaolin Zhang, Weiqi Nian, Krishna Chaitanya Pavani, Zhifeng Li, Xingsheng Wang, Haibo Wu
Summary: The research reveals that the miR-342-3p/SOCS6 axis plays a crucial role in regulating anti-Mtb immunity and the balance between cell apoptosis and necrosis during Mtb infection, providing new insights for developing host-directed therapies targeting these pathways.
Article
Medicine, Research & Experimental
Yueying Li, Zhan Zhang, Ke Xu, Shuang Du, Xiaosong Gu, Rangjuan Cao, Shusen Cui
Summary: Minocycline shows promising potential in preventing or alleviating peripheral nerve adhesion through anti-inflammatory, anti-fibrosis, and myelination protective effects. The study reveals that minocycline promotes regulatory macrophage polarization while inhibiting pro-inflammatory macrophage polarization, suggesting its effectiveness in treating adhesion-related disorders. Mechanism studies also indicate that minocycline regulates the TAK1 pathway to reduce inflammatory factor expression and enhance anti-inflammatory cytokine expression.
Article
Immunology
Tuhina Gupta, Demba Sarr, Kayla Fantone, Nuha Milad Ashtiwi, Kaori Sakamoto, Frederick D. Quinn, Balazs Rada
Summary: Mtb is the primary cause of human tuberculosis, but the role of Duox1 in bacterial infections remains largely unknown. This study showed that in a mouse model, Duox1 is dispensable for the overall clinical course of Mtb lung infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Neurosciences
Qingxiu Yao, Hui Wang, Hengchao Chen, Zhuangzhuang Li, Yumeng Jiang, Zhipeng Li, Jiping Wang, Yazhi Xing, Feng Liu, Dongzhen Yu, Shankai Yin
Summary: The study identified SPTAN1 as a critical molecule for HC stereocilia morphology and auditory function through regulation of focal adhesion signaling. Sptan1 deficiency in mice led to deafness, abnormal formation of stereocilia and cuticular plates.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Endocrinology & Metabolism
Fan Wang, Juanjuan Zou, Huajun Xu, Weijun Huang, Xiaoman Zhang, Zhicheng Wei, Xinyi Li, Yupu Liu, Jianyin Zou, Feng Liu, Huaming Zhu, Hongliang Yi, Jian Guan, Shankai Yin
Summary: Chronic intermittent hypoxia and chronic sleep fragmentation are two main pathological features of obstructive sleep apnea. This study found correlations between intestinal microbiota, metabolites, and lipid metabolism under conditions of CIH or CSF, which affected adipose tissue and hepatic lipid metabolism.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Niannian Li, Zhenfei Gao, Jinhong Shen, Yuenan Liu, Kejia Wu, Jundong Yang, Shengming Wang, Xiaoman Zhang, Yaxin Zhu, Jingyu Zhu, Jian Guan, Feng Liu, Shankai Yin
Summary: The study investigates the impact of m6A modification in severe OSA, identifying dysregulated m6A regulators in severe OSA patients and proposing tailored treatment strategies based on different subclusters. Functional analysis and assessment of immune response activity provide critical insights for future research and treatment options.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Multidisciplinary
Zhenfei Gao, Anzhao Wang, Yongxu Zhao, Xiaoxu Zhang, Xiangshan Yuan, Niannian Li, Chong Xu, Shenming Wang, Yaxin Zhu, Jingyu Zhu, Jian Guan, Feng Liu, Shankai Yin
Summary: This study identified the substrates of BTBD9-mediated ubiquitination through proteomic analysis and ubiquitinome analysis. Among the potential substrates, IMPDH2 was identified as a novel target and may be associated with sleep dysregulation.
Article
Pathology
Maoxiang Xu, Shengming Wang, Yumeng Jiang, Jingjing Wang, Yuanping Xiong, Wenqi Dong, Qingxiu Yao, Yazhi Xing, Feng Liu, Zhengnong Chen, Dongzhen Yu
Summary: Single-cell RNA sequencing was used to analyze the cellular composition of vestibular schwannomas (VSs) and investigate the intercellular communication between Schwann cells (SCs) and tumor-associated fibroblasts. The study identified heterogeneous SC subtypes and revealed an important role of integrin and growth factor signaling in the communication between SCs and fibroblasts. These findings provide insights into the development of VSs.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Yiming Cai, Qixin Zhu, Jianhong Shi, Cheng Li, Dapeng Yan, Jinyan Li, Yingbin Xing
Summary: We present the results of high-power laser output obtained using a 300-mu m-core-diameter Yb-doped fiber. With the use of an all-fiber construction and pulse control system, this laser system achieves high performance in terms of average power and peak power, setting a new record.
IEEE PHOTONICS JOURNAL
(2022)
Article
Multidisciplinary Sciences
Xiaokai Zhang, Tingrong Xiong, Lin Gao, Yu Wang, Luxuan Liu, Tian Tian, Yun Shi, Jinyong Zhang, Zhuo Zhao, Dongshui Lu, Ping Luo, Weijun Zhang, Ping Cheng, Haiming Jing, Qiang Gou, Hao Zeng, Dapeng Yan, Quanming Zou
Summary: This study reveals a previously unrecognized mechanism by which Staphylococcus aureus suppresses host defense, involving the secretion of extracellular fibrinogen-binding protein (Efb) into macrophages. Efb disrupts the formation of the TRAF3/TRAF2/cIAP1 complex, leading to the suppression of the inflammatory signaling cascade and decreased pathogenicity.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Yao Wang, Ran Cao, Chen Wang, Xiyu Song, Ruina Wang, Jiacun Liu, Mengmeng Zhang, Junyi Huang, Tingting You, Yihua Zhang, Dapeng Yan, Wendong Han, Lan Yan, Jisheng Xiao, Peng Li
Summary: A method for preparing highly effective and sustainable antimicrobial nanofibers is reported, which embeds photoactive hydrogen-bonded organic framework (HOF) of rod-like nanocrystals. The resulting nanofibers exhibit excellent tensile and breathability characteristics while protecting HOF nanocrystals against corrosion. By optimizing the amount and type of embedded nanocrystals, the efficiency of singlet oxygen (O-1(2)) generation is improved. The HOF@PVDF-HFP nanofibers demonstrate highly effective killing of pathogens, including viruses, bacteria, and fungi, within 30 minutes under ambient light conditions.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell Biology
Gui Qian, Yihua Zhang, Yinan Liu, Manman Li, Bowen Xin, Wenyi Jiang, Wendong Han, Yu Wang, Xian Tang, Liuyan Li, Lingyan Zhu, Tao Sun, Bo Yan, Yongtang Zheng, Jianqing Xu, Baoxue Ge, Zheng Zhang, Dapeng Yan
Summary: Cyclic GMP-AMP synthase (cGAS) recognizes Y-form cDNA of HIV-1 and initiates antiviral immune response, but the p6 protein of HIV-1 suppresses IFN-I expression and promotes immune evasion. Glutamylated p6 inhibits STING interaction with TRIM32 or AMFR, suppressing STING activation by inhibiting K27 and K63 polyubiquitination at K337. CoCl2, a CCP agonist, counteracts p6 glutamylation and inhibits HIV-1 immune evasion.
Article
Cell Biology
Haipeng Liu, Hang Su, Fei Wang, Yifang Dang, Yijiu Ren, Shenyi Yin, Huinan Lu, Hang Zhang, Jun Wu, Zhu Xu, Mengge Zheng, Jiani Gao, Yajuan Cao, Junfang Xu, Li Chen, Xiangyang Wu, Mingtong Ma, Long Xu, Fang Wang, Jianxia Chen, Chunxia Su, Chunyan Wu, Huikang Xie, Jijie Gu, Jianzhong Jeff Xi, Baoxue Ge, Yiyan Fei, Chang Chen
Summary: Enhancing chemosensitivity is a critical need in cancer therapy. Through a screening process, we have identified brivanib as a modulator of cGAS, which enhances the immune response in tumor cells treated with platinum-based chemotherapeutics. This finding suggests the potential of combining brivanib with platinum-based drugs for cancer treatment.
Article
Neurosciences
Anzhao Wang, Zhicheng Wei, Haolin Yuan, Yaxin Zhu, Yu Peng, Zhenfei Gao, Yuenan Liu, Jinhong Shen, Huajun Xu, Jian Guan, Shankai Yin, Feng Liu, Xinyi Li
Summary: This study investigates the association of single nucleotide polymorphisms (SNPs) in the FKBP5 gene with obstructive sleep apnea (OSA) and OSA-related traits. The results show that four SNPs in the FKBP5 gene are associated with sleep respiratory events in patients with moderate-to-severe OSA during REM sleep, as well as sleep architecture variables in men with moderate OSA. FKBP5 variants may be a potential predisposing factor for sleep disorders.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Physics, Multidisciplinary
Yiming Cai, Yifan Mai, Shen Xiang, Jianhong Shi, Qixin Zhu, Rong Li, Jinyan Li, Cheng Li, Dapeng Yan, Yingbin Xing
Summary: In this study, we successfully transmitted a 100 MW-peak-power ultrafast laser through a 5-m anti-resonant hollow-core fiber (AR-HCF) with a pumpable armored tube for air exhaust. The results highlight the significance of controlling air concentration in achieving high transmission efficiency and pulse quality.
FRONTIERS IN PHYSICS
(2023)
Article
Immunology
Jianxia Chen, Fen Tang, Haohao Li, Xiangyang Wu, Yong Yang, Zhonghua Liu, Xiaochen Huang, Jie Wang, Ruijuan Zheng, Lin Wang, Haipeng Liu, Junfang Xu, Peng Wang, Feng Liu
Summary: This study reveals a significant decrease in Aplp2 levels in TB patients, M.tb-infected macrophages, and mice. Knockdown or mutation of Aplp2 leads to reduced macrophage-mediated killing of M.tb, accompanied by decreased expression of iNOS and reduced cytokine production. Aplp2 mutant mice show increased susceptibility to mycobacterial infection.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Letter
Biochemistry & Molecular Biology
Tiansheng Zheng, Haipeng Liu, Yifan Hong, Yajuan Cao, Qing Xia, Chengge Qin, Ming Li, Russel J. J. Reiter, Yidong Bai, Lihong Fan
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biochemical Research Methods
Fei Wang, Ming Tong Ma, Junfang Xu, Haipeng Liu
Summary: The protocol describes a method to monitor PARP1 subcellular localization and details steps to identify phosphorylation sites on PARP1. This has implications for studying protein phosphorylation events in other cell types.
