Journal
JOURNAL OF IMMUNOLOGY
Volume 198, Issue 11, Pages 4235-4243Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1602169
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Funding
- Netherlands Organisation for Health Research and Development TOP Grant [91211011]
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Dendritic cells (DCs) are specialized in Ag engulfment via a wide variety of uptake receptors on their cell surface. In the present study we investigated Ag uptake and presentation of in vivo-formed Ag-Ab complexes by i.v. injecting mice with Ag-specific Abs followed by the cognate Ag. We show by this natural Ab-mediated Ag targeting system that uptake by splenic APC subsets is severely hampered in mice lacking complement factor C1q (C1qa (-/-)). Moreover, no detectable Ag cross-presentation by CD8 alpha(+) DCs from C1qa (-/-) mice was found. On the contrary, Ag uptake was not hampered by APCs in FcgRI/II/III/IV-deficient (FcgR quadruple (-/-) ) mice, and the cross-presentation ability of CD8 alpha(+) DCs was not affected. In conclusion, we show that C1q rather than FcgRs controls the Ab-mediated Ag uptake and its presentation by spleen APC subsets to T cells.
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