Article
Genetics & Heredity
Indira Bag, Yang Chen, Karole D'Orazio, Prisma Lopez, Sabine Wenzel, Yuichiro Takagi, Elissa P. Lei
Summary: Chromatin insulators are DNA-protein complexes that establish independent transcriptional domains in the genome. This study reveals a novel RNA-binding protein, Isha, that interacts with the su(Hw) mRNA and associates with the gypsy chromatin insulator complex. Isha interacts with RNA Polymerase II and is involved in the RNA-dependent chromatin association at the 5' end of genes. Depletion of Isha disrupts the enhancer-blocking and barrier activities of the gypsy insulator, as well as the nuclear localization of insulator bodies.
G3-GENES GENOMES GENETICS
(2022)
Article
Cell Biology
Kara Stark, Olivia Crowe, Lindsay Lewellyn
Summary: Intercellular bridges are crucial for fertility in many organisms, with the fruit fly egg serving as a model system for studying these structures. The study demonstrates a novel role for the Dock protein in regulating the size and stability of intercellular bridges in the germline, providing insights into the mechanisms underlying these processes.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Nadezhda E. Vorobyeva, Maksim Erokhin, Darya Chetverina, Alexey N. Krasnov, Marina Yu Mazina
Summary: Su(Hw) is an insulator protein involved in regulating chromatin architecture and gene repression in Drosophila. It plays a crucial role in the developmental amplification of 66D and 7F Drosophila amplicons by establishing a chromatin structure that is permissive to pre-RC recruitment.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Marta Marzullo, Giulia Romano, Claudia Pellacani, Federico Riccardi, Laura Ciapponi, Fabian Feiguin
Summary: Aging modifies the physiological balance of the organism, increasing susceptibility to neurodegenerative diseases. This is related to epigenetic chromatin remodeling events that affect the expression levels of disease-causing genes and neuronal survival.
CELL DEATH DISCOVERY
(2023)
Article
Genetics & Heredity
J. Keller McKowen, Satya V. S. P. Avva, Mukesh Maharjan, Fabiana M. Duarte, Jacob M. Tome, Julius Judd, Jamie L. Wood, Sunday Negedu, Yunkai Dong, John T. Lis, Craig M. Hart
Summary: The study reveals that BEAF physically interacts with the Pbro subunit of PBAP, which plays a subtle role in gene activation and chromatin structure. The interaction between BEAF and Pbro is more important in regulated genes, while other redundant factors may mask their roles in BEAF-associated promoters.
G3-GENES GENOMES GENETICS
(2022)
Review
Neurosciences
Thomas C. Dockendorff, Mariano Labrador
MOLECULAR NEUROBIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Todd Schoborg, Mariano Labrador
CELLULAR AND MOLECULAR LIFE SCIENCES
(2014)
Article
Biochemistry & Molecular Biology
Maria Piedad Plata, Hyuck Joon Kang, Shaofei Zhang, Srilalitha Kuruganti, Shih-Jui Hsu, Mariano Labrador
Article
Biochemistry & Molecular Biology
Heather A. Wallace, Maria P. Plata, Hyuck-Joon Kang, Misty Ross, Mariano Labrador
Article
Genetics & Heredity
Mariano Labrador, Ky Sha, Alice Li, Victor G. Corces
Article
Cell Biology
Todd Schoborg, Ryan Rickels, Josh Barrios, Mariano Labrador
JOURNAL OF CELL BIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Todd A. Schoborg, Mariano Labrador
JOURNAL OF MOLECULAR EVOLUTION
(2010)
Article
Multidisciplinary Sciences
Todd Schoborg, Srilalitha Kuruganti, Ryan Rickels, Mariano Labrador
Article
Genetics & Heredity
Emily C. Stow, James R. Simmons, Ran An, Todd A. Schoborg, Nastasya M. Davenport, Mariano Labrador
Summary: Insulators play important roles in genome structure and function in eukaryotes. The different roles of insulator protein partners in the Drosophila genome and how they confer functional specificity remain poorly understood.
Article
Biology
Bright Amankwaa, Todd Schoborg, Mariano Labrador
Summary: Mounting evidence suggests that liquid-liquid phase separation (LLPS) plays a crucial role in the formation and dissolution of membraneless intracellular organelles. Insulator proteins, including the cohesin subunit Rad21, are involved in the assembly of insulator bodies, which exhibit characteristics of LLPS.
LIFE SCIENCE ALLIANCE
(2022)
Article
Genetics & Heredity
James R. Simmons, Ran An, Bright Amankwaa, Shannon Zayac, Justin Kemp, Mariano Labrador
Summary: Insulator proteins and phosphorylated histone variant H2Av are associated with each other and play a role in gene transcription regulation. The study found that in Drosophila, insulator components colocalize with the phosphorylated H2Av throughout the genome and are enriched at TAD boundaries, suggesting their important role in boundary formation.
Article
Biochemistry & Molecular Biology
Shih-Jui Hsu, Emily C. Stow, James R. Simmons, Heather A. Wallace, Andrea Mancheno Lopez, Shannon Stroud, Mariano Labrador
Article
Biochemistry & Molecular Biology
M Labrador, VG Corces
Article
Cell Biology
M Labrador, VG Corces
GENES & DEVELOPMENT
(2003)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)