Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 50, Issue 2, Pages 139-145Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2015.02.005
Keywords
Tribolium castaneum; Bacillus thuringiensis; Defensin; Apolipophorin III; Innate immunity; Cry toxin receptors
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Funding
- Ministerio de Ciencia e Innovacion
- Fondo Europeo de Desarrollo Regional (FEDER) [BIO 2007-67860, AGL2010-22300-C03-03]
- Conselleria Valenciana d'Educacio i Ciencia [BFPI/2008/145]
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In Tribolium castaneum larvae we have demonstrated by RNA interference knockdown that the Bacillus thuringiensis Cry3Ba toxin receptors Cadherin-like and Sodium solute symporter proteins are also functional receptors of the less active Cry3Aa toxin. Differences in susceptibility to B. thuringiensis infection might not only rely on toxin-receptor interaction but also on host defense mechanisms. We compared the expression of the immune related genes encoding Apolipophorin-Ill and two antimicrobial peptides, Defensin3 and Defensin2 after B. thuringiensis challenge. All three genes were up-regulated following Cry3Ba spore-crystal intoxication whereas only Defensins gene expression was induced upon Cry3Aa spore-crystal treatment, evidencing a possible association between host immune response and larval susceptibility to B. thuringiensis. We assessed the antimicrobial activity spectra of T. castaneum defensins peptide fragments and found that a peptide fragment of Defensin3 was effective against the human microbial pathogens, Escherichia coil, Staphylococcus aureus and Candida albicans, being S. aureus the most susceptible one. (C) 2015 Elsevier Ltd. All rights reserved.
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