Journal
JOURNAL OF FOOD BIOCHEMISTRY
Volume 41, Issue 5, Pages -Publisher
WILEY
DOI: 10.1111/jfbc.12395
Keywords
enrichment of polyunsaturated fatty acids; immobilization; lipase; N-succinyl-chitosan beads
Funding
- International cooperation project of Ministry of Science and Technology of China [2014DFA32880]
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Bovine pancreatic lipase was immobilized onto N-succinyl-chitosan beads by using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC)/N-hydroxysuccinimide (NHS) as the activating agent. The immobilized lipase was characterized by Fourier transform infrared spectra and scanning electron microscopy. The activity of the immobilized lipase obtained under the optimized immobilization conditions was found to reach 1,480 U/g, with enzyme activity recovery of 74.5%. Compared to the free lipase, thermal stability of the immobilized lipase was greatly improved. The reusability study indicated that the immobilized lipase retained 76.6% of its initial activity after being recycled five times. A preliminary study indicated that the prepared immobilized lipase can effectively enrich the polyunsaturated fatty acid in fish oil (increasing from 45.1 to 67.7%), of which the combined docosahexaenoic acid and eicosapentaenoic acid contents increased from 16.3 to 27.1%.
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