Article
Fisheries
Xiaoyan Li, Rzgar Jaafar, Yang He, Boqian Wu, Per Kania, Kurt Buchmann
Summary: The study investigated the potential of Pseudomonas H6 lipopeptide as a surfactant in preventing parasitic infection in rainbow trout fry caused by Ichthyophthirius multifiliis. It was found that the surfactant could prevent infection and inflammation caused by the parasite exposure in a certain concentration.
Article
Fisheries
Chiara Pasqualetti, Jacob Gunther Schmidt, Alessandra Cafiso, Leandro Gammuto, Olivia Lanzoni, Dagoberto Sepulveda, Amedeo Manfrin, Lisandro Benedetti Cecchi, Niels Jorgen Olesen, Chiara Bazzocchi, Giulio Petroni
Summary: Red mark syndrome (RMS) is a disease affecting farmed rainbow trout at temperatures below 16 degrees C, leading to downgrading of the product and economic losses. The causative agent, likely a bacterium called RMS-Midichloria like organism (RMS-MLO), is still unclear. Research suggests that the protozoan, Ichthyophthirius multifiliis, may play a role in transmitting RMS-MLO bacteria.
Article
Marine & Freshwater Biology
Weiguang Kong, Guangyi Ding, Gaofeng Cheng, Peng Yang, Zhen Xu
Summary: This study reveals the previously unknown immune defense role of the fish eye mucosa by developing an infection model of rainbow trout OM. It is found that parasite infection can up-regulate immune-related genes and cause pathological changes in ocular structures.
MARINE LIFE SCIENCE & TECHNOLOGY
(2023)
Article
Fisheries
Moonika Haahr Marana, Azmi Al-Jubury, Heidi Mathiessen, Kurt Buchmann
Summary: The lipopeptide SPH6 derived from Pseudomonas H6 has surfactant properties and exhibits lethal effects on the external stages of the problematic gill parasitic ciliate Ichthyopthirius multifiliis. The mode of action involves surfactant attachment and penetration of the parasite plasma membrane, leading to detachment of cilia.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Azmi Al-Jubury, Fei Yin, Taghrid Abusharkh, Mehedi Hasan Fuad, Per Walter Kania, Kurt Buchmann
Summary: This study found that brass and its pure constituents (copper and zinc) have in vivo and in vitro antiparasitic effects on freshwater parasites. The results showed that brass and pure copper had a 100% mortality rate on parasites within 15 minutes, while zinc had a 100% mortality rate within 30 minutes. The study also found that direct contact with the metal sheets increased the parasiticidal effect. However, further research is needed to investigate the environmental and ecological effects of metal sheet use.
Article
Fisheries
Zhao Zhang, Haibo Cui, Zhiqiang Zhang, Shenye Qu, Gaoxue Wang, Fei Ling
Summary: The study investigated the pharmacokinetics of magnolol in goldfish following different administration routes and found that oral administration of magnolol effectively controlled Ichthyophthirius multifiliis infection. The results indicated that magnolol could be a potential treatment for fish infections caused by I. multifiliis.
Article
Fisheries
Heidi Mathiessen, Rzgar Mohammad Jaafar, Azmi Al-Jubury, Louise von Gersdorff Jorgensen, Per Walter Kania, Kurt Buchmann
Summary: The study demonstrates that feed additives including garlic, oregano, thyme, coriander, and astaxanthin can inhibit the early development of the skin parasitic ciliate Ichthyophthirius multifiliis in Rainbow Trout, both directly and indirectly by enhancing the host immune response. Garlic showed the highest parasiticidal effect in vitro, while oregano and thyme also induced significant regulation of immune genes in trout leukocytes.
NORTH AMERICAN JOURNAL OF AQUACULTURE
(2021)
Article
Microbiology
Ze-Yi Cao, Bing-Wen Xi, Qing-Jie Zhou, Kai Chen, Jun Xie
Summary: White spot disease, caused by the parasitic ciliate Ichthyophthirius multifiliis, is a significant threat to the freshwater fish farming industry worldwide. The presence of zooplankton, specifically cyclopoid copepods, in the culture water body can significantly reduce the infection of I. multifiliis in goldfish. This study suggests that cyclopoid copepods have the potential to be used as biological control agents against white spot disease in aquaculture.
Article
Fisheries
Gao-Feng Cheng, Wei-Guang Kong, Xue Zhai, Qing-Jiang Mu, Zhao-Ran Dong, Meng-Ting Zhan, Zhen Xu
Summary: CD79a and CD79b are important components in rainbow trout, showing high expression in response to bacterial, parasitic, and viral infections in skin, gills, and head kidney. Their vital roles in both systemic and mucosal immune responses during infections contribute to the exploration of CD79 subunits in B cell signaling in ontogeny and disease.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Banikalyan Swain, Cole T. Powell, Roy Curtiss
Summary: In this study, we have successfully developed a RAEV vector system capable of synthesizing protective antigen IAG52B of Ichthyophthirius multifiliis (Ich) for the vaccination of susceptible fish against edwardsiellosis and white spot disease. This vaccine delivery system features a bacteria strain with genomic deletions of asdA, which is essential for the synthesis of a component of Gram-negative bacteria cell wall. The system also exhibits regulated-delayed attenuation in vivo, allowing for controlled synthesis of recombinant antigens and prevention of disease symptoms. Our findings demonstrate the potential of this vaccine-vector system in providing protection against various bacterial, viral, and parasitic infectious diseases in teleost fish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
HyeongJin Roh, Nameun Kim, Yoonhang Lee, Jiyeon Park, Bo Seong Kim, Mu Kun Lee, Chan-Il Park, Do-Hyung Kim
Summary: During infection, a total of 6,097 and 2,931 differentially expressed genes (DEGs) were identified in the head kidney and liver, respectively. Markers of protein processing, oxidative phosphorylation, and the proteasome were highly expressed in the liver. Interestingly, immune-relevant pathways were highly activated in the liver, but down-regulated in the head kidney, suggesting the importance of interpreting co-expression among organs for profiling systemic responses during infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Ke Huang, Guangran Hu, Runqiu Wang, Qingwen Zeng, Wenxiang Li, Hong Zou, Shangong Wu, Guitang Wang, Ming Li
Summary: Berberine has strong toxic effects on the theronts of I. multifiliis, but has no effect on the protomonts. It can reduce the release of theronts and change the shape of protomonts. Berberine could be an effective and safe potential parasiticide for killing I. multifiliis.
Article
Veterinary Sciences
K. Puk, L. Guz
Summary: Ichthyophthiriasis, caused by Ichthyophthirius multifiliis infections, has a severe impact on freshwater aquaculture productivity. Malachite green, previously used to treat these infections, is now banned due to carcinogenicity. Tanacetum vulgare extract shows promise as a new and efficacious drug to control Ich.
POLISH JOURNAL OF VETERINARY SCIENCES
(2021)
Article
Fisheries
Koumei Shiota, Masaki Sukeda, Harsha Prakash, Masakazu Kondo, Teruyuki Nakanishi, Takahiro Nagasawa, Miki Nakao, Tomonori Somamoto
Summary: This study investigates local immune responses to Ichthyophthirius multifiliis using intra-fin administration in freshwater teleosts. The results suggest that the parasite antigens inhibit macrophages and neutrophils migration, while T-cells are the primary responders. Further research should focus on exploring inhibitory factors of the parasite or examining innate functions of teleost T-cells.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
M. Heidarieh, M. Akbari, H. Unger
Summary: The study compared gene expression levels of MHC-Pi, IL-8, and IgM in rainbow trout vaccinated with gamma-irradiated trophonts against Ichthyophthirius multifiliis. Results showed up-regulation of these genes in vaccinated fish with the potential to enhance mucosal and systemic immune responses.
INTERNATIONAL JOURNAL OF RADIATION RESEARCH
(2021)
Review
Parasitology
J. T. Timi, K. Buchmann
Summary: Fish parasitological research associated with fisheries and aquaculture has significantly expanded over the past century. The application of parasites as biological tags has provided new insights into fish migration and stock assessments globally. Recent findings include the extension of parasite tags to hosts other than bony fishes, improvements in parasite tag selection, recognition of host traits affecting their use, and the need for integrative multidisciplinary studies combining parasites with classical and modern techniques.
JOURNAL OF HELMINTHOLOGY
(2023)
Article
Immunology
Louise Goksoyr, Magdalena Skrzypczak, Maureen Sampson, Morten A. Nielsen, Ali Salanti, Thor G. Theander, Alan T. Remaley, Willem A. De Jongh, Adam F. Sander
Summary: Administration of PCSK9-specific monoclonal antibodies and peptide-based PCSK9 vaccines can reduce plasma LDL cholesterol by blocking PCSK9. However, these treatments also increase plasma PCSK9 levels due to the formation of immune complexes. A study using a cVLP-based vaccine platform shows that the FL PCSK9 cVLP vaccine has an enhanced ability to opsonize and clear plasma PCSK9. These findings have implications for the design of vaccines that can effectively mediate opsonization and immune clearance of target antigens.
Article
Infectious Diseases
Merel J. Smit, Adam F. Sander, Maud B. P. A. Ariaans, Cyrielle Fougeroux, Constanze Heinzel, Rolf Fendel, Meral Esen, Peter G. Kremsner, Rob ter Heine, Heiman F. Wertheim, Manja Idorn, Alexander P. Underwood, Alekxander Binderup, Santseharay Ramirez, Jens Bukh, Max Soegaard, Sayit M. Erdogan, Tobias Gustavsson, Stine Clemmensen, Thor G. Theander, Lancet Microbe, Ali Salanti, Mette Hamborg, Willem A. de Jongh, Matthew B. B. McCall, Morten A. Nielsen, Benjamin G. Mordmuller
Summary: A clinical trial was conducted in the Netherlands to test a cVLP-based COVID-19 vaccine. The results showed that the vaccine exhibited high immunogenicity at antigen levels of 25-70 mu g and had high virus neutralization activity against major SARS-CoV-2 variants.
Review
Parasitology
Kurt Buchmann
Summary: The Baltic Sea, a young and ecologically unstable marine ecosystem, exhibits the influence of evolutionary and ecological processes on host-parasite interactions. The increased population of Baltic grey seals has led to higher infection rates of Baltic cod with anisakid nematode larvae. These parasites can be transmitted to humans and pose a risk to human health.
FOLIA PARASITOLOGICA
(2023)
Article
Immunology
Jeong In Yang, Dagoberto Sepulveda, Irina Vardia, Jakob Skov, Louise Goksoyr, Adam F. F. Sander, Niels Lorenzen
Summary: This study aimed to develop and test a recombinant alternative vaccine based on virus-like particles (VLPs) decorated with VapA protein as the key structural surface protein for combating A. salmonicida bacterial disease. The results showed that rainbow trout vaccinated with VapA-VLP vaccines exhibited comparable protection to the bacterin-based vaccine and produced a strong VapA-specific antibody response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Heidi Mathiessen, Yajiao Duan, Moonika H. Marana, Shaozhi Zuo, Asma M. Karami, Rzgar Jafaar, Louise von Gersdorff Jorgensen, Per W. Kania, Inger Dalsgaard, Lone Madsen, Torben Nielsen, Fabian Grammes, Jorgen odegard, Valeria Macchia, Kurt Buchmann
Summary: Flavobacterium psychrophilum is the causative agent of Bacterial Cold Water Disease (BCWD)/ Rainbow Trout Fry Syndrome (RTFS) in rainbow trout. The disease causes significant mortality in trout farms and leads to economic losses in the aquaculture industry. The immature development of adaptive immunity in young life cycle stages hinders successful vaccination, resulting in the widespread use of antibiotics. Selective breeding and identification of markers associated with disease resistance may improve breeding efforts for more resilient strains.
AQUACULTURE REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Jessica Pihl, Thomas M. M. Clausen, Jiarong Zhou, Nishta Krishnan, Maj S. S. Orum-Madsen, Tobias Gustavsson, Robert Dagil, Mads Daugaard, Swati Choudhary, Camilla Foged, Jeffrey D. D. Esko, Liangfang Zhang, Ronnie H. H. Fang, Ali Salanti
Summary: Malaria-infected erythrocytes and oncofetal CS have distinct tropism and can potentially be used for cancer targeting. Researchers have developed a drug delivery platform using erythrocyte membrane-coated nanoparticles that mimic malaria-infected erythrocytes and specifically target and kill melanoma cells. This biomimetic approach shows potential as a broadly targeted cancer therapy.
Article
Veterinary Sciences
Justin Doritchamou, Morten A. Nielsen, Arnaud Chene, Nicola K. Viebig, Lynn E. Lambert, Adam F. Sander, Jean-Philippe Semblat, Sophia Hundt, Sachy Orr-Gonzalez, Christoph Mikkel Janitzek, Alicia J. Spiegel, Stine B. Clemmensen, Marvin L. Thomas, Martha C. Nason, Maryonne Snow-Smith, Emma K. Barnafo, Joseph Shiloach, Beth B. Chen, Steven Nadakal, Kendrick Highsmith, Tarik Ouahes, Solomon Conteh, Ankur Sharma, Holly Torano, Brandi Butler, Karine Reiter, Kelly M. Rausch, Puthupparampil V. Scaria, Charles Anderson, David L. Narum, Ali Salanti, Michal Fried, Thor G. Theander, Benoit Gamain, Patrick E. Duffy
Summary: This study investigated the functional antibody response and antibody boosting during PM episodes in Aotus monkeys immunized with placental malaria vaccine (PMV) candidates. The results showed that PMV candidates induced functional antibodies with limited heterologous activity in the monkeys, similar to human responses. The Aotus model appears to be suitable for preclinical downselection of PMV candidates and assessment of antibody boosting.
Article
Immunology
Heidi Mathiessen, Sebastian Kjeldgaard-Nintemann, Carlota Marola Fernandez Gonzalez, Cyril Henard, Julie Algreen Reimer, Sara Vebaek Gelskov, Moonika Haahr Marana, Louise von Gersdorff Jorgensen
Summary: This study investigated the early immune responses in zebrafish larvae infected with the parasite Ichthyophthirius multifiliis. The results showed that the larvae exhibited mild inflammation upon infection, and the 12-day-old larvae were less susceptible compared to the 5-day-old larvae. Neutrophils and macrophages were attracted to the parasites and exhibited neutrophil extracellular traps (NETs) during the fight against the parasite. The parasite employed rotation as an immune evasive strategy, but occasionally, neutrophils and macrophages were able to kill the parasite. In conclusion, the zebrafish larvae responded with localized high activity from neutrophils and macrophages, but only displayed mild inflammation systemically.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Julie Van Coillie, Tamas Pongracz, Tonci Sustic, Wenjun Wang, Jan Nouta, Mathieu Le Gars, Sofie Keijzer, Federica Linty, Olvi Cristianawati, Jim B. D. Keijser, Remco Visser, Lonneke A. van Vught, Marleen A. Slim, Niels van Mourik, Merel J. Smit, Adam Sander, David E. Schmidt, Maurice Steenhuis, Theo Rispens, Morten A. Nielsen, Benjamin G. Mordmueller, Alexander P. J. Vlaar, C. Ellen van der Schoot, Ramon Roozendaal, Manfred Wuhrer, Gestur Vidarsson
Summary: In this study, the glycosylation profiles of anti-S IgG1 Fc in response to mRNA, adenoviral, and protein-based COVID-19 vaccines were compared using mass spectrometry (MS). The results showed variations in the glycosylation patterns between different vaccine platforms and individuals, but overall, vaccine-induced anti-S IgG glycosylation was dynamic with marked overlaps.
Article
Fisheries
Azmi Al-Jubury, Fei Yin, Taghrid Abusharkh, Mehedi Hasan Fuad, Per Walter Kania, Kurt Buchmann
Summary: This study found that brass and its pure constituents (copper and zinc) have in vivo and in vitro antiparasitic effects on freshwater parasites. The results showed that brass and pure copper had a 100% mortality rate on parasites within 15 minutes, while zinc had a 100% mortality rate within 30 minutes. The study also found that direct contact with the metal sheets increased the parasiticidal effect. However, further research is needed to investigate the environmental and ecological effects of metal sheet use.
Article
Veterinary Sciences
Asma Mohammad Karami, Moonika Haahr Marana, Heidi Mathiessen, Inger Dalsgaard, Torben Fejer Nielsen, Per Walter Kania, Kurt Buchmann
Summary: This study demonstrates the feasibility of marker assisted selective breeding of fish resistant to vibriosis, a bacterial disease in rainbow trout farming. A specific single nucleotide polymorphism marker (SNP AX-89945921) associated with resistance was validated. By selecting male fish carrying this marker and breeding them with outbred female trout, a population with higher resistance to the disease was obtained. The QTL fish showed slower and lower morbidity compared to the non-QTL fish when exposed to the bacterium.
ACTA VETERINARIA SCANDINAVICA
(2023)
Article
Ecology
Wan Muhammad Hazim Wan Sajiri, Csaba Szekely, Kalman Molnar, Kurt Buchmann, Boglarka Sellyei
Summary: The lifecycle of Thaparocleidus vistulensis, a parasite of European catfish, was studied through observation of infection dynamics, egg development, hatching rates, and survival rates at different life stages. The data obtained allows for predictions of parasite-host dynamics and may contribute to the control of gill-disease in cultured European catfish stocks.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE
(2023)
