Article
Cell Biology
Sandeep Gupta, Riki Kawaguchi, Eric Heinrichs, Salena Gallardo, Stephanie Castellanos, Igor Mandric, Bennett G. Novitch, Samantha J. Butler
Summary: This study successfully generated a large quantity of bona fide somatosensory interneurons using mouse embryonic stem cells. The findings suggest that retinoic acid and bone morphogenetic protein 4 can induce the differentiation of cells into different types of spinal interneurons, and activating Wnt signaling can enhance neural progenitor proliferation and yield.
Article
Biochemistry & Molecular Biology
Fuqiang Cui, Xiaoxiao Li, Wenwu Wu, Wenbo Luo, Ying Wu, Mikael Brosche, Kirk Overmyer
Summary: MYB108/BOS1 enhances cell death in the gain-of-function mutant bos1-1 after fungal infection and mechanical wounding. Programmed cell death (PCD) is integral to plant life and required for stress responses, immunity, and development. Our understanding of the regulation of PCD is incomplete, especially concerning regulators involved in multiple divergent processes. The botrytis-susceptible (bos1) mutant of Arabidopsis is highly susceptible to fungal infection by Botrytis cinerea (Botrytis). BOS1 (also known as MYB108) regulates cell death propagation during plant responses to wounding. The bos1-1 allele contains a T-DNA insertion in the 5 '-untranslated region upstream of the start codon. This insertion results in elevated expression of BOS1/MYB108. We used clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated nuclease 9 (Cas9) system (CRISPR/Cas9) to create new bos1 alleles with disrupted exons, and found that these lines lacked the typical bos1-1 wounding and Botrytis phenotypes. They did exhibit reduced fertility, as was previously observed in other bos1 alleles. Resequencing of the bos1-1 genome confirmed the presence of a mannopine synthase (MAS) promoter at the T-DNA left border. Expression of the BOS1 gene under control of the MAS promoter in wild-type plants conferred the characteristic phenotypes of bos1-1: Botrytis sensitivity and response to wounding. Multiple overexpression lines demonstrated that BOS1 was involved in regulation of cell death propagation in a dosage-dependent manner. Our data indicate that bos1-1 is a gain-of-function mutant and that BOS1 function in regulation of fertility and Botrytis response can both be understood as misregulated cell death.
Article
Multidisciplinary Sciences
Juan Pablo Lopez, Elena Brivio, Alice Santambrogio, Carlo De Donno, Aron Kos, Miriam Peters, Nicolas Rost, Darina Czamara, Tanja M. Brueckl, Simone Roeh, Max L. Poehlmann, Clara Engelhardt, Andrea Ressle, Rainer Stoffel, Alina Tontsch, Javier M. Villamizar, Martin Reincke, Anna Riester, Silviu Sbiera, Martin Fassnacht, Helen S. Mayberg, W. Edward Craighead, Boadie W. Dunlop, Charles B. Nemeroff, Mathias Schmidt, Elisabeth B. Binder, Fabian J. Theis, Felix Beuschlein, Cynthia L. Andoniadou, Alon Chen
Summary: Chronic activation and dysregulation of the neuroendocrine stress response can have severe physiological and psychological consequences, with the molecular characterization of the hypothalamic-pituitary-adrenal axis revealing a previously unreported subpopulation of cells involved in stress adaptation. This research suggests that modulating ABCB1 function may be important in developing treatment strategies for patients with metabolic and stress-related psychiatric disorders.
Article
Biochemistry & Molecular Biology
Hyo-Jin Park, Seul-Gi Yang, Deog-Bon Koo
Summary: The study examined the role of SESN2/NRF2 signaling in regulating oxidative stress and ER stress during porcine oocyte maturation. Activation of p-NRF2(Ser40) in the nucleus of porcine oocytes was accompanied by downregulation of PERK signaling. Knockdown of SESN2 gene expression disrupted NRF2 signaling activation and affected spindle assembly in porcine oocytes. This suggests that the PERK/SESN2/NRF2 signaling pathway may play a crucial role in protecting against ER stress during meiotic maturation and oocyte development.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Cell Biology
Sandhini Saha, Rohit Verma, Chandan Kumar, Bhoj Kumar, Amit Kumar Dey, Milan Surjit, Sivaram V. S. Mylavarapu, Tushar Kanti Maiti
Summary: This study used a proteomics approach to investigate the mechanism of lipotoxicity in hepatocellular carcinoma cells, revealing that ectopic lipid accumulation severely affects the ubiquitin-proteasomal system and that palmitic acid downregulates USP7, leading to cell mitosis. The research also found altered expression of cell cycle checkpoint proteins, disrupting early G2/M checkpoint recovery and inducing mitotic catastrophe resulting in hepatocyte death. Additionally, palmitic acid preferentially induced cell death through AIF-mediated apoptosis by depolarizing mitochondria and translocating AIF to the nucleus.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Kai Song, Chao Liu, Jiashuai Zhang, Yang Yao, Huiting Xiao, Rongqiang Yuan, Keru Li, Jia Yang, Wenyuan Zhao, Yanqiao Zhang
Summary: This study identified 12 functional miRNAs associated with metabolic colorectal cancer (CRC) and revealed the important role of miR-20a in promoting the progression of metabolic CRC by regulating fatty acid metabolism. The study suggests that miR-20a could be a potential therapeutic target for preventing tumor metastasis in CRC.
Article
Biochemistry & Molecular Biology
Vidya Mangala Prasad, Daniel P. Leaman, Klaus N. Lovendahl, Jacob T. Croft, Mark A. Benhaim, Edgar A. Hodge, Michael B. Zwick, Kelly K. Lee
Summary: This study used cryo-electron tomography to analyze Env in HIV-1 particles and revealed distinct positioning of Env in immature particles relative to the underlying Gag lattice. Additionally, unexpected structural features of virion-bound Env, such as a variable central core and heterogeneous glycosylation, were discovered. These findings provide a comprehensive understanding of HIV assembly and structural variation in Env antigen presentation.
Article
Chemistry, Physical
Yixuan Han, Connie B. Roth
Summary: The study utilized a linear gradient model to fit ellipsometric data for thin polymer films, revealing more physically realistic refractive index values compared to the commonly used homogeneous Cauchy layer model. The direction of refractive index gradient indicated a higher density near the free surface, contrary to common expectations, leading to insights on faster free surface dynamics needed for optimized denser molecular packings in stable glasses. Additionally, the magnitude of refractive index gradient varied across different polymer films, with P2VP films showing a more muted response possibly due to decoupling in free surface and substrate dynamics.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Daniel Uysal, Karl-Friedrich Kowalewski, Maximilian Christian Kriegmair, Ralph Wirtz, Zoran Popovic, Philipp Erben
Summary: Technological advances in molecular profiling have enabled the identification of 8q22.2 as a potentially prognostic gene region in MIBC, with OSR2 identified as a potential biomarker for survival prognosis. Multiple genetic levels, including DNA copy number alterations and mRNA gene expression, are important in comprehensive molecular characterization of cancer genomic regions.
Article
Parasitology
Canglin Zhang, Chunhai Luo, Rui Yang, Yaming Yang, Xiaofang Guo, Yan Deng, Hongning Zhou, Yilong Zhang
Summary: This study identified multiple species of Anopheles mosquitoes in the Cambodia-Laos border region, with morphological identification showing higher accuracy for dominant species and molecular identification showing lower accuracy for other species. The combination of molecular methods and morphological analysis can provide a better understanding of malaria transmission factors and intervention effects.
PARASITES & VECTORS
(2022)
Article
Veterinary Sciences
Ali Mazloum, Antoinette van Schalkwyk, Andrey Shotin, Nikolay Zinyakov, Alexey Igolkin, Roman Chernishev, Zoran Debeljak, Fedor Korennoy, Alexander V. Sprygin
Summary: Since the first report of African swine fever (ASF) outbreaks in Georgia in 2007, the disease has spread rapidly across Europe, Russia, and Asia. This study examines ASFV isolates from the Russian region of Kaliningrad and finds that they are closely related to isolates from Poland and Germany. A unique mutation at a specific locus was identified, providing an additional marker for distinguishing these isolates.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Carleen Mae Sabusap, Disha Joshi, Luba Simhaev, Kathryn E. Oliver, Hanoch Senderowitz, Marcel van Willigen, Ineke Braakman, Andras Rab, Eric J. Sorscher, Jeong S. Hong
Summary: Patients with cystic fibrosis carrying the P67L variant in CFTR often exhibit typical CF symptoms and show robust response to CFTR correctors. Biochemical measurements and molecular dynamics analysis suggest that the P67L mutation shares key pathogenic features with F508del and may impair the integrity of CFTR structure, providing a potential target for pharmacological repair.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yuta Yamamoto, Shogo Nakano, Fumio Seki, Yasuteru Shigeta, Sohei Ito, Hiroaki Tokiwa, Makoto Takeda
Summary: The infection of hosts by morbilliviruses relies on the interaction between viral hemagglutinin and SLAM receptors. While the importance of the n-terminal region of human SLAM as a measles virus receptor has been demonstrated, its role in infection by other morbilliviruses and host range determination is still unclear due to the region's high flexibility. By using computational chemistry methods, the study showed that a specific residue in the N-terminal region of SLAM from Macaca is essential for the stable interaction with CDV-H, providing insights into CDV infection in Macaca. This approach could aid in determining molecular interactions involving flexible protein regions that are challenging to study using traditional methods.
Article
Biochemistry & Molecular Biology
Priyanka Basak, Diane E. Cabelli, Peter T. Chivers, Erik R. Farquhar, Michael J. Maroney
Summary: This study reveals the involvement of L-histidine in the protease-mediated maturation of nickel-dependent superoxide dismutase (NiSOD), and demonstrates that L-histidine can serve as a metallochaperone to facilitate nickel incorporation.
Article
Hematology
Zu-Lin Chen, Pradeep Kumar Singh, Katharina Horn, Sidney Strickland, Erin H. Norris
Summary: Alzheimer's disease is a neurodegenerative disorder that leads to dementia. Inhibiting the activation of the plasma contact activation system may be beneficial for Alzheimer's patients, and a potential strategy has been proposed.
Article
Cell & Tissue Engineering
Melany Jackson, Antonella Fidanza, A. Helen Taylor, Stanislav Rybtsov, Richard Axton, Maria Kydonaki, Stephen Meek, Tom Burdon, Alexander Medvinsky, Lesley M. Forrester
Summary: The Apelin receptor plays a crucial role in the production of HSPCs from mesodermal cells and its signaling pathway is required for their generation. However, the activation of APLNR signaling impairs the maintenance of long-term reconstituting HSPCs and drives myeloid differentiation, suggesting a dual role in hematopoiesis.
Editorial Material
Dentistry, Oral Surgery & Medicine
David P. Rice
EUROPEAN JOURNAL OF ORTHODONTICS
(2022)
Book Review
Dentistry, Oral Surgery & Medicine
David P. Rice
EUROPEAN JOURNAL OF ORTHODONTICS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Sirpa Arte, Minna Poyhonen, Emmi Myllymaki, Elisa Ronkainen, David P. Rice, Pekka Nieminen
Summary: This study aimed to investigate the genetic basis and craniofacial and dental features of Finnish patients with Axenfeld-Rieger syndrome (ARS). Mutational analyses revealed frameshift mutations and whole gene deletions in these patients, and they displayed a class III skeletal relationship, retrognathic maxilla, anterior crossbite, and tooth agenesis, among other dental abnormalities.
ORTHODONTICS & CRANIOFACIAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Yuliya Linhares, Alexander Kaganski, Christian Agyare, Isil A. Kurnaz, Vidushi Neergheen, Bartlomiej Kolodziejczyk, Monika Kedra, Muhammad Wahajuddin, Lahcen El-Youssf, Thomas Edison dela Cruz, Yusuf Baran, Milica Pesic, Uttam Shrestha, Rigers Bakiu, Pierre-Marie Allard, Stanislav Rybtsov, Myrtani Pieri, Velia Siciliano, Yensi Flores Bueso
Summary: Biodiversity is crucial for the robustness, stability, and re-silience of ecosystems, which in turn provide essential services for human survival. However, human activity is causing a rapid decline in biodiversity, posing a threat to the planet's life-support systems. This article emphasizes the importance of biodiversity for human health, particularly in the field of biomedicine, and presents approaches to promote biodiversity conservation.
TRENDS IN MOLECULAR MEDICINE
(2023)
Article
Biology
Dzhina D. Karal-ogly, Alexander N. Shumeev, Viktoria V. Keburiya, Marina V. Mintel, Stanislav A. Rybtsov
Summary: Non-anthropoid primates cynomolgus monkeys (Macaca fascicularis), also known as crab-eating macaques, are used in biomedical and preclinical studies due to their similarities to humans in terms of diet, infectious diseases, and senile conditions. However, the age-related changes and sexual dimorphism of the immune system in these monkeys have not been extensively studied, despite their significant impact on disease progression and medication response.
Article
Dentistry, Oral Surgery & Medicine
Myllymaki Emmi, Heikinheimo Kaisa, Suominen Auli, Evalahti Marjut, Michelotti Ambra, Svedstrom-Oristo Anna-Liisa, Rice P. David
Summary: This study aimed to determine the impact of malocclusion and orthodontic treatment on symptoms of temporomandibular joint disorder (TMD). The findings suggest that crossbite is associated with self-reported TMJ sounds, while orthodontic treatment does not affect the number of symptoms.
JOURNAL OF ORAL REHABILITATION
(2023)
Article
Cell Biology
Md. Rakibul Hasan, Anna Koskenranta, Kirsi Alakurtti, Maarit Takatalo, David P. P. Rice
Summary: RAB23 plays a crucial role in musculoskeletal development by regulating the formation of the patella bone and maintenance of tendon progenitors. Its loss results in abnormal formation of the patella and knee joint. RAB23 is required for the specification of Sox9 and scleraxis double-positive chondroprogenitors during patella formation and establishment of the patellofemoral joint. Furthermore, RAB23 is involved in TGF beta superfamily signaling, which is important for patella initiation and progenitor differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Takashi Ono, Valmy Pangrazio-Kulbersh, Letizia Perillo, Flavia Artese, Ewa Czochrowska, M. Ali Darendeliler, Steven Dugoni, William E. Fayad Bajaire, Eric Liou, Jae Hyun Park, David Rice, Abbas Zaher, Athanasios E. Athanasiou, Lee W. Graber, Nikhilesh R. Vaid
Summary: The World Federation of Orthodontists (WFO) has emphasized its support for recognized orthodontic specialty training programs worldwide and has recently revised and updated its educational guidelines to reflect the expanded scope and demands of orthodontic education and practice. The aim of these guidelines is to provide minimum program requirements to prepare orthodontic specialty residents to deliver the best level of treatment.
JOURNAL OF THE WORLD FEDERATION OF ORTHODONTISTS
(2023)
Editorial Material
Dentistry, Oral Surgery & Medicine
David P. Rice
EUROPEAN JOURNAL OF ORTHODONTICS
(2023)
Review
Biochemistry & Molecular Biology
Yuliya S. Martyshkina, Valeriy P. Tereshchenko, Daria A. Bogdanova, Stanislav A. Rybtsov
Summary: The accumulation of senescent adaptive immunity cells in the elderly is a growing concern due to the increased risk of global epidemics. Elderly individuals are more susceptible to infections and age-related diseases, with a higher risk of vaccination failure. The accumulation of senescent cells leads to increased inflammation, causing lymphocyte exhaustion and various diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Daniil Shevyrev, Valeriy Tereshchenko, Tatiana N. Berezina, Stanislav Rybtsov
Summary: Hematopoietic stem cells (HSCs) support hematopoiesis throughout life, but their regenerative potential decreases with age, affecting immune function. The aging of HSCs leads to decreased self-renewal ability, changes in differentiation potential, and a disturbed immune cell formation. This results in increased inflammation and higher risk of age-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Psychology, Clinical
Tatiana N. Berezina, Stanislav A. Rybtsov
Summary: Individual hobbies and interests, as well as the ways of spending leisure time, have an impact on the development of personal resources, which in turn influence health and wellbeing. Through the assessment of personal resources and their correlation with biological age, a trained neural network has been used to predict resources that slow down aging. While women share common resources such as nature, intellect, and achievements in reducing biological aging, no common resources have been found for men. However, individual selection of resources can still predict the ability to slow down aging.
EUROPEAN JOURNAL OF INVESTIGATION IN HEALTH PSYCHOLOGY AND EDUCATION
(2022)
Meeting Abstract
Hematology
Edie Crosse, Anahi Binagui-Casas, Tamir Chandra, Sabrina Gordon-Keylock, Alexander Medvinsky, Stanislav Rybtsov
EXPERIMENTAL HEMATOLOGY
(2022)
Article
Psychology, Clinical
Anna V. Koteneva, Tatiana N. Berezina, Stanislav A. Rybtsov
Summary: The challenges of modern civilization lead to premature aging among professionals, with religiosity and spirituality having an influence on psychobiological age. However, this area remains understudied in psychology.
EUROPEAN JOURNAL OF INVESTIGATION IN HEALTH PSYCHOLOGY AND EDUCATION
(2021)
