Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 799, Issue -, Pages 111-121Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2017.05.048
Keywords
Electrochemical immunosensor; Electrode polarization; Biotin; Infant formulas; Serum; HPLC
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Funding
- University of Malaya Research Grant (UMRG) [RP012C-14SUS]
- Fundamental Research Grant Scheme (FRGS) from the Ministry of Higher Education of Malaysia (MOHE) [FP041-2016]
- Postgraduate Research Fund (PPP) from the University of Malaya [PG007-2015B]
- MOHE
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Development of a simple electrochemical immunosensor, for the direct detection of biotin, is important for monitoring biotin content. A displacement assay was used in biotin detection, in which surface-bound antibodies were dissociated from the surface of immunosensor in the presence of free biotin. Pre-treatment of samples is not required for biotin detection using this electrochemical immunosensor. Using this electrochemical immunosensor, the recoveries of biotin in the two infant formulas A and B were 93.9% and 91.3%, respectively, of their biotin concentrations as stated on their packaging. The direct detection of biotin, with this electrochemical immunosensor, in supplements X, Y, and Z, showed recoveries as high as 92.9%, 106.5%, and 100.0%, respectively. The accuracy of our electrochemical immunosensor was validated with high pressure liquid chromatography (HPLC). The surface of immunosensor had a strong anti-fouling property and high specificity for actual applications in complex matrices. Additionally, the developed immunosensor shows good stability, reproducibility, and intra- and inter-day precision. This electrochemical immunosensor can directly detect biotin in infant formulas, biotin-containing supplements, and serum.
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