Article
Biochemical Research Methods
I. G. Bressan, S. F. Llesuy, C. Rodriguez, A. Ferloni, A. R. Dawidowski, S. B. Figar, M. Gimenez
Summary: In 2015, glyphosate was classified as probably carcinogenic to humans by the International Agency for Research on Cancer, leading to increased public concerns about its environmental and health risks. A simple LC-MS/MS method was developed for quantification of glyphosate in human urine and successfully applied to 52 urine samples from exposed subjects in northern Argentina.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Veterinary Sciences
Yong Wu, Xue-Ming Long, Gao-Feng Liu, Xia Bai, Zhi-Liang Sun, Zhao-Ying Liu
Summary: The study aimed to investigate tissue residue depletion in pigs fed G. elegans powder. Two quantitation methods, including a precise method and a semi-quantitation method, were developed and validated for the analysis of multiple components in pig tissues. The results showed that G. elegans alkaloids were widely distributed in pig tissues, and potential residue markers and target tissues were identified. The findings provide important scientific evidence for evaluating the safety of animal-derived food from G. elegans.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemical Research Methods
Tengfei Yuan
Summary: In this study, a sample-multiplexing LC-MS/MS method was developed and validated for the quantification of serum estrogens. The method demonstrated high accuracy and precision, and allowed simultaneous analysis of E1, E2, and E3.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Suji Kim, Min Seo Lee, Mingyu Kim, Beom Jun Ko, Hye Suk Lee, Sooyeun Lee
Summary: This study aimed to develop a sensitive and accurate method for the simultaneous determination of endogenous gamma-hydroxybutyric acid (GHB) and its metabolites in human urine. LC-MS/MS was used to quantify the concentrations, and chemical derivatization with benzoyl chloride was employed to improve the sensitivity for certain compounds. Additionally, the effects of handling and storage conditions of urine samples on metabolite production and degradation were observed.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jiangshuo Li, Jing Xu, Ruiping Zhang, Jiuming He, Manjiangcuo Wang, Guanggen Jiao, Zeper Abliz
Summary: In this study, a targeted strategy for screening fatty acids was developed using parallel labeling and high-resolution mass spectrometry analysis. The method was successfully applied to determine fatty acid levels in plasma samples from esophageal squamous cell carcinoma patients and healthy controls, revealing six altered fatty acids affected by tumor invasion. This method provides insights into the identification of unknown fatty acids and has potential applications in clinical diagnosis and the study of disease mechanisms.
Article
Veterinary Sciences
Nattha Jariyapamornkoon, Koranis Patthanachai, Nipattra Suanpairintr
Summary: This study aimed to determine the pharmacokinetic parameters of oral Fosfomycin tromethamine in canine plasma and urine using LC-MS/MS. The results indicated that oral Fosfomycin as the tromethamine salt was better absorbed into the blood circulation compared to disodium salt. The high urine Fosfomycin concentrations suggest that it could be a viable substitute for bacterial cystitis treatment in dogs with multidrug resistant infections.
VETERINARY SCIENCES
(2023)
Article
Chemistry, Analytical
Xiaona Li, Zhini Liu, Zhuo Li, Xin Xiong, Xianhua Zhang, Changqing Yang, Libo Zhao, Rongsheng Zhao
Summary: A novel LC-MS method was developed for simultaneous quantification of 16 purine metabolites in human plasma and urine. The method showed good linearity, low quantitation limits, and stable recovery and matrix effects. The method was successfully applied to compare the plasma and urine samples of patients without and with acute kidney injury (AKI), revealing potential upregulation of certain purine intermediates in AKI patients, indicating an association with inflammatory responses.
Article
Biochemical Research Methods
Honoka Kaneko, Hiroki Matsuoka, Takayuki Ishige, Hironori Kobayashi, Tatsuya Higashi
Summary: This study developed an improved derivatization method combined with LC/ESI-MS/MS for the sensitive and specific quantification of serum/plasma E-2. The new method reduced the reaction time and interference peaks compared to the previous method, making it a sensitive and specific alternative for clinical analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ziming Wang, Yu Wang, Tao Yu, Zhiwei Hu, Yang Wang
Summary: In this study, a method for quantifying lupeol in rat plasma was developed using liquid chromatography-electrospray ionization/tandem mass spectrometry. The method showed high accuracy and precision, as well as stability under experimental conditions. It was successfully applied to the pharmacokinetic study of lupeol in rat plasma after oral administration.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Chemistry, Applied
Ye Li, Jun Liang, Jia-Ning Gao, Yu Shen, Hai-Xue Kuang, Yong-Gang Xia
Summary: A new method combining liquid chromatography-tandem mass spectrometry (LC-MS/MS) and aldononitrile acetate derivatization (AND) has been developed for the comprehensive characterization of monosaccharide composition in plant derived polysaccharides. The optimized method can effectively separate 17 carbohydrates and is more powerful than traditional GLC-based methods for analyzing complex natural polysaccharides.
CARBOHYDRATE POLYMERS
(2021)
Review
Chemistry, Analytical
Lili Zhang, Na Li, Shengshuang Chen, Xiqing Bian, Mohamed A. Farag, Yahui Ge, Jianbo Xiao, Jian-Lin Wu
Summary: Carboxyl-containing compounds (CCCs) are crucial for the human body and their determination in food is challenging. Derivatization LC-MS technique plays a significant role in overcoming these challenges in food chemistry. This review comprehensively discusses CCCs in terms of structures, biological functions, and food sources, highlighting the importance of derivatization LC-MS as a powerful analytical tool.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Hsin-Yu Liao, Chin-Yi Wang, Ching-Hua Lee, Hsien-Li Kao, Wei-Kai Wu, Ching-Hua Kuo
Summary: A sensitive and effective LC-MS/MS method was developed to simultaneously quantify gut microbiota-related metabolites in human plasma. The study revealed a significant decrease in plasma butyrate levels in CVD patients, suggesting a potentially protective role in CVD.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Chemistry, Analytical
Apostolia Tsiasioti, Paraskevas D. Tzanavaras
Summary: Glutathione, as the most abundant antioxidant in living organisms, plays a crucial role in cells due to its free thiol group. Its determination using liquid chromatography has been widely studied and proves significant in understanding the development of human diseases.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Mengxue Li, Ping Li, Yuting Ji, Yihua Tian, Huiying Zeng, Xiaoyan Chen
Summary: A method for simultaneous determination of 10 monosaccharides in rat plasma using LC-MS/MS was developed and validated. The method employed PMP as a derivatization reagent, improving the chromatographic retention and ionization efficiency of monosaccharides. The method was linear and met the accuracy and precision criteria. It was used to study the effect of hepatic insufficiency on monosaccharide levels in rats.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Leandro Francisco Pippa, Maria Paula Marques, Anna Christina Tojal da Silva, Fernando Crivelenti Vilar, Tissiana Marques de Haes, Benedito Antonio Lopes da Fonseca, Roberto Martinez, Eduardo Barbosa Coelho, Lauro Wichert-Ana, Vera Lucia Lanchote
Summary: This study developed LC-MS/MS methods for quantifying AmB in different matrices in neurocryptococcosis patients receiving treatment, successfully validating their applicability. Pharmacokinetic analysis revealed changes in drug clearance, volume of distribution, and unbound concentration of AmB at the initiation and conclusion of treatment in the assessed patient.
FRONTIERS IN CHEMISTRY
(2021)