Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1064, Issue -, Pages 56-61Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2017.09.001
Keywords
DPP-IV inhibitory peptides; Purification; Molecular docking; Antarctic krill; Diabetes mellitus
Funding
- Modern Agro-industry Technology Research System of China [CARS-47]
- Discipline Construction Project of Institutions of Higher Learning in Guangdong Province of China [2013CXZDA020]
- Guangdong Ocean University Strong School Innovation Fund Project [GDOU2014050203, GDOU2013050314]
- Industry-university-research Cooperation Project in Guangdong Province of China [20138090600155]
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Dipeptidyl peptidase IV (DPP-IV) played an important role in blood glucose regulation. Inhibition of DPP-IV may improve glycemic control in diabetics by preventing the rapid breakdown of incretin hormones and prolonging their physiological action. In this study, Antarctic krill (Euphausia superba) protein was hydrolyzed using animal proteolytic enzymes. The hydrolysate was purified sequentially by ultrafiltration, gel filtration chromatography and reversed phase high-performance liquid chromatography (RP-HPLC). DPP-IV inhibitory activity of the fractions achieved. from Antarctic krill protein was determined by DPP-IV screening reagent kit. Two purified peptides were identified by Xevo G2-XS QTof mass spectrometer (QTOF-MS). One peptide purified was Ala-Pro (AP) with IC50 values of 0.0530 mg/mL, the other Ile-Pro-Ala (IPA) with IC50 values of 0.0370 mg/mL. They both exhibited strong DPP-IV inhibitory activity. The molecular docking analysis revealed that DPP-IV inhibition by AP and IPA was mainly due to formation of a strong interaction surface force with the 91-96 and 101-105 amino acids of the DPP-IV. Our results suggested that the protein hydrolysate from Antarctic krill can be considered as a promising natural source of DPP-IV inhibitory peptides in the management of diabetes.
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