Article
Green & Sustainable Science & Technology
Shanshan Xu, Hanxiang Li, Gonglei Wang, Zhiping Liu, Man Long, Jinsong Guo, Peng Yan, Youpeng Chen, Fang Fang
Summary: This study investigated the effects of long-term exposure to low-concentration polystyrene nanoplastics on anammox granular sludge. The results showed that such exposure led to a decrease in nitrogen removal performance and granule stability. Additionally, the oxidative stress level of cells increased, leading to sludge cracking and reduced strength, as well as inhibiting the growth of certain bacterial strains. This research is crucial for assessing the potential risk of long-term PS-NP exposure and maintaining high nitrogen removal performance and stability.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Engineering, Environmental
Yun Li, Nan Cui, Keng Xuan, Dan Xu, Dongliang Wang, Chaoming Li, Zebing Li, Yufan Wang
Summary: This study evaluated the start-up performance and process kinetics of a UASB-Anammox reactor with nitritation sludge at low substrate concentration. It was found that the reactor could be successfully started by inoculating with a mixture of nitritation sludge and anaerobic ammonium oxidation granular sludge, achieving good nitrogen removal. The analysis using microbial growth kinetics models and process kinetics provided useful parameters for predicting nitrogen removal performance, operation management, and control of similar reactors.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Environmental
Zijian Chen, Fangang Meng, Chuyuan Zhou, Xiaowei Wu, Chao Jin
Summary: Adaptation to substrate fluctuations is a life reality for microbes in global municipal wastewater treatment plants. However, there is still a lack of definite information on how influent changes with different alteration frequencies shape the stability of anammox consortia and their metabolic regulations.
Article
Agricultural Engineering
Dong Li, Yiwen Dong, Shuai Li, Pengfei Jiang, Jie Zhang
Summary: This article explores the use of biochar and increased HRT to enhance the recovery of stored anaerobic ammonia oxidation granular sludge. The results show that biochar accelerates the recovery process, shortening the time needed for nitrogen removal and re-granulation. It also promotes the growth of Anammox bacteria and improves the stability of the bioreactor. The optimized community structure of biochar makes the system more resistant to risks.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Nian-Si Fan, Jin-Jin Fu, Dong-Qi Huang, Yuan-Long Ma, Zheng-Yang Lu, Ren-Cun Jin, Ping Zheng
Summary: High concentrations of antibiotics ETC and SMZ had significant inhibitory effects on the nitrogen removal performance of the anammox process, with the combined inhibitory effect being more significant. The anammox process was able to quickly recover from deterioration, possibly attributed to the increase in ARGs and secretion of EPS.
Article
Agricultural Engineering
Victory Fiifi Dsane, Haejun Jeon, Yuri Choi, Sohee Jeong, Younggyun Choi
Summary: Magnetite is an iron-rich carrier that can enhance the activity and aggregation of anammox bacteria. In this study, different-sized samples from a granulation reactor were used, and it was found that larger granules had higher iron content and improved microbial cell density. The specific anammox activity increased with granule size, and the heme c content was also higher in larger granules. The extracellular polymeric substances (EPS) analysis showed differences in secretion intensity and protein structures among the different sizes. The relative abundance of Brocadia Sinica also increased with granule size. This study highlights the importance of in-depth EPS analysis beyond traditional yield and major content analysis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Wenqiang Wang, Dong Li, Shuai Li, Huiping Zeng, Jie Zhang
Summary: The characteristics and mechanism of hollow anammox granular sludge with different settling property were investigated. The analysis revealed that the deterioration of settling performance was associated with an increase in extracellular polymeric substances (EPS) content. The microstructure showed significant changes in the number of pores for different types of granules. EPS was found to be a key driving force in the floating and proliferation of hollow anammox granules, while EPS-hydrolyzing bacteria weakened the structural strength of the granules.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Environmental Sciences
Jin-Jin Fu, Dong-Qi Huang, Yu-Hui Bai, Yang-Yang Shen, Xia-Zhen Lin, Yong Huang, Yi-Rong Ling, Nian-Si Fan, Ren-Cun Jin
Summary: The study demonstrates the resistance of the anammox process to multiple antibiotics by producing antibiotic resistance genes and secreting extracellular polymeric substances. Molecular docking simulations reveal potential binding sites between ammonium transporter and different antibiotics, supporting the stable operation of the anammox system under antibiotic stress.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Dongmin Yang, Cancan Jiang, Shengjun Xu, Likun Gu, Danhua Wang, Jialiang Zuo, Huacai Wang, Shujun Zhang, Dongsheng Wang, Huanzhen Zhang, Xuliang Zhuang
Summary: This study investigates the nitrogen removal performance and the effects of extracellular polymeric substances (EPS) on anammox activity in two different reactors. It is found that anaerobic ammonium oxidation (Anammox) technology shows promising results in treating low C/N ratio wastewater. EPS has a significant impact on the activity of anammox bacteria, with the addition of Anammox-EPS contributing to better nitrogen removal capability. Different feeding strategies influence the granulation style and further affect the activity and stability of the system.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Environmental
Zhiju Liu, Yujie Qin, Xiangyin Liu, Lan Yang, Siyuan Gong, Shaohong Zheng, Xinyue Lin, Junfeng Yang, Tiansheng Lu, Jiannv Chen
Summary: The effects of substance concentration and glycine betaine on nitrogen removal and microbial communities in the sulfide autotrophic denitrification and Anammox coupling process were investigated. It was found that increasing substance concentration decreased the efficiency of ammonia and total nitrogen removal, but adding glycine betaine improved the removal efficiency. High-throughput sequencing results showed that increasing substrate concentration led to a decrease in Anammox bacteria abundance and an increase in Thiobacillus abundance.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Yeonju Kim, Jaecheul Yu, Soyeon Jeong, Jeongmi Kim, Seongjae Park, Hyokwan Bae, Sung-Keun Rhee, Tatsuya Unno, Shou-Qing Ni, Taeho Lee
Summary: This study evaluated the effect of Ca2+ and Mg2+ augmentation on the recovery of freshwater anammox granules affected by salinity stress. The results showed that adding Ca2+ and Mg2+ at optimal concentrations increased the granules' activity and EPS production. Salinity stress did not significantly affect the microbial community in the granules. The alleviation effect of divalent cations on salinity stress might be associated with increased EPS production rather than changes to the anammox bacteria.
APPLIED SCIENCES-BASEL
(2022)
Article
Engineering, Environmental
Roent Dune A. Cayetano, Gi-Beom Kim, Jong-Hun Park, Min-Jae Lee, Sang-Hyoun Kim
Summary: The study demonstrated the benefits of biomass retention through dynamic membrane (DM) for stable performance of AnDMBR treating waste activated sludge, with an observed shift in archaeal population and functional gene expression at higher substrate concentrations. The presence of glycosyltransferase encoding genes in the biofilm formation process and the increase in average particle size distribution suggests the potential for high-rate treatment of waste activated sludge using an AnDMBR system.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Engineering, Environmental
Hong Wang, Hong Chen, Shiyu Liu, Lei Li, Donghai Yang, Xiaohu Dai
Summary: This study investigates the characteristics and differences in performance inhibition and recovery of the anammox process under substrate inhibition and dissolved oxygen (DO) exposure. The results show that DO exposure has a higher effect on anammox activity compared to substrate inhibition. Additionally, the dominant anammox genus changes after DO exposure. Increasing the nitrogen loading rate by using a low-concentration influent and short HRT can effectively avoid substrate inhibition and enhance nitrogen removal performance.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Engineering, Environmental
Yi Xue, Haiyuan Ma, Zhe Kong, Yu-You Li
Summary: The study investigated the role of EPS and HAP core in Ca and P removal in anammox-HAP granules, showing that the addition of granule crystal seeds can accelerate the anammox process and enhance overall treatment efficiency.
Article
Engineering, Environmental
Weigang Wang, Yayi Wang
Summary: This study investigates the segregation mechanism of granules and flocs in anammox granular systems by comparing the chemical composition, physical properties, spectral characteristics, and adherence properties of extracellular polymeric substances (EPS) extracted from anammox granules (Granule-EPS) and flocs (Floc-EPS). The results show that Granule-EPS has a more complex texture, larger biomolecule size, and higher molecular weight compared to Floc-EPS. Floc-EPS also exhibits weaker co-aggregation ability and looser adherence layer structure than Granule-EPS, which contributes to the lower sludge intensity of anammox flocs compared to granules.
CHEMICAL ENGINEERING JOURNAL
(2023)
