Review
Nutrition & Dietetics
Magdalena Kulus, Maurycy Jankowski, Wieslawa Kranc, Afsaneh Golkar Narenji, Maryam Farzaneh, Piotr Dziegiel, Maciej Zabel, Pawel Antosik, Dorota Bukowska, Paul Mozdziak, Bartosz Kempisty
Summary: In vitro meat production is a potential alternative to traditional meat consumption, offering a product that is nearly identical to conventional meat in terms of nutrition and taste. However, current alternative products often lack the same nutritional value and culinary attributes as animal-derived meats, posing challenges for global acceptance. This review examines the history of cultured meat, current cell sources and production methods, focusing on the role of bioreactors, scaffolds, and microcarriers in overcoming these obstacles. The authors discuss the potential impact of microcarrier and scaffold-based solutions, as well as the practical and societal barriers to widespread implementation of this technology as a viable food source.
FRONTIERS IN NUTRITION
(2023)
Article
Polymer Science
Lixia Huang, Yifei Jiang, Xinying Chen, Wenqi Zhang, Qiuchen Luo, Siyan Chen, Shuhan Wang, Fangqing Weng, Lin Xiao
Summary: Supramolecular responsive microcarriers based on chitosan microspheres were developed for nonenzymatic cell detachment. These microcarriers showed excellent biocompatibility and gelatin niches, allowing for effective cell attachment and expansion. Cells could be detached from the microcarriers through competitive binding.
Article
Engineering, Environmental
Xiaolei Chen, Dong Zhang, Xinqing Wang, Zhenxi Liu, Huili Kang, Changsheng Liu, Fangping Chen
Summary: In this study, a low-cost, easy-assembly, and scalable method for preparing GelMA PMS for bone marrow mesenchymal stem cells (BMSCs) expansion is proposed. The pore size of GelMA PMS can be controlled by adjusting the freezing temperature. GelMA PMS exhibited higher cell attachment ratio, cell spreading area, and differentiation potential compared to commercial microcarriers.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Rodrigo G. Rosado-Castillo, Carlos G. Aguilar-Madera, Julio C. Sacramento Rivero, Sergio A. Baz-Rodriguez
Summary: The upscaling of gas-liquid mass transfer and bioreaction in aerated cultures was studied using the volume averaging theory. Effective transport equations were derived for the main substrates and biomass, as well as the gaseous substrate. Two approaches were used for the transferable component: local mass equilibrium and the two-fluid model.
CHEMICAL ENGINEERING SCIENCE
(2023)
Review
Engineering, Biomedical
Changmin Shao, Junjie Chi, Luoran Shang, Qihui Fan, Fangfu Ye
Summary: Droplet microfluidic technology is a new platform for controlling the generation of microdroplets and droplet-derived materials. It has unique advantages in the preparation of functional microcarriers and is widely used in the areas of cell culture, tissue engineering, and drug delivery.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Physical
Lingjiao Zou, Weiqing Zhou, Xiangming Na, Naoki Asakawa, Hua Yue, Guanghui Ma
Summary: 3D cell carriers with porous structures have the potential to investigate drug treatments for tumors. The fabrication of gigantic highly-porous microcarriers allows for high-efficiency 3D cell culture and the construction of artificial tumor tissue models.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Alvin W. Nienow, Halina Murasiewicz, Andrzej W. Pacek, Christopher J. Hewitt, Karen Coopman
Summary: The study demonstrated the use of perfluorocarbon liquid microcarriers for expanding and recovering human mesenchymal stem/stromal cells, showing that cells can successfully attach, proliferate, and maintain quality on these liquid carriers.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Erin L. Roberts, Sarah I. M. Lepage, Thomas G. Koch, Michael S. Kallos
Summary: This study investigated the development of an expansion process using Vertical-Wheel bioreactors for the cultivation of equine cord blood-derived MSCs. The results showed that VW bioreactors with appropriate microcarriers could produce large numbers of cells with reproducibility and homogeneity, making them suitable for cell therapy.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Materials Science, Biomaterials
Raja Vadivelu, Navid Kashaninejad, Mohammad Reza Nikmaneshi, Rubina Rahaman Khadim, Seyedeh Sarah Salehi, Naveen Chintala Ramulu, Yasuyuki Sakai, Masaki Nishikawa, Bahar Firoozabadi, Nam-Trung Nguyen
Summary: A functional sessile LM system for long-term 3D cell culture is developed by embedding LM with agarose gel to reduce evaporation. Comprehensive mathematical modeling, numerical simulations, and experimental investigations show that the platform acts as a moisture absorption system to control evaporation, extending the life span of LMs. The presence of agarose significantly contributes to minimizing evaporation and improving the viability of the harvested multicellular spheroids.
Review
Engineering, Chemical
Brigitte Altmann, Christoph Gruen, Cordula Nies, Eric Gottwald
Summary: This article provides a detailed description of the research status of 3D cell culture in micro-bioreactors, highlighting different types of micro-bioreactors and their applications. It is noteworthy that micro-bioreactors have been utilized in SARS-CoV research prior to the SARS-CoV2 pandemic, and the complexity levels in the systems were defined based on the corresponding 3D cell culture techniques applied.
Article
Biotechnology & Applied Microbiology
Jonathan Jones, Didier Kindembe, Harvey Branton, Najib Lawal, Eduardo Lopez Montero, John Mack, Shuo Shi, Ron Patton, Gary Montague
Summary: This paper describes the development of improved control strategies for the standard environmental conditions in a fed-batch bioreactor used for monoclonal antibody cell culture. The consequences of relying on fixed parameter PID based controllers are considered and poor performance is demonstrated as a consequence of non-linearity and loop interactions. The benefits from adopting a more sophisticated control strategy are considered.
FOOD AND BIOPRODUCTS PROCESSING
(2023)
Review
Biotechnology & Applied Microbiology
Chunyan Duan, Mingjia Yu, Changji Hu, Hongying Xia, Ranjith Kumar Kankala
Summary: Tissue engineering aims to restore tissue defects using 3D biomimetic pre-formed scaffolds. Micro-sized injectable modularized units have gained interest as ideal delivery vehicles for cells and growth factors due to their minimally-invasive nature and improved cell retention efficacy. This review discusses the progress of these carriers in tissue engineering, highlighting their design and ability to deliver cells and promote tissue growth.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Rosa M. Coco-Martin, Cristina Andres-Iglesias, Girish K. Srivastava, Javier Lagos-Rodriguez, Miguel Ruiz-Tevah, Mario R. Diaz-Cardenas, Ivan Fernandez-Bueno, Juan Garcia-Serna, Maria T. Garcia-Gutierrez, Alfredo Garcia-Layana, J. Carlos Pastor
Summary: The study reported cases of acute intraocular toxicity caused by MEROCTANE commercialized by a Turkish company, highlighting the cytotoxic effects and presence of highly toxic contaminants. While perfluorocarbon liquids are useful tools for intraocular surgery, measures must be implemented to ensure product safety based on both chemical and cytotoxicity analysis for every batch, and medical staff should report suspected cases to the respective National Agencies.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Janna N. Sloand, Tyler E. Culp, Nichole M. Wonderling, Enrique D. Gomez, Scott H. Medina
Summary: Nature has developed elegant strategies to organize inert building blocks into adaptive supramolecular structures, with interfacial self-assembly being one favored approach. By exploiting antithetical fluorous-water interfaces, non-canonical fluorinated amino acids can be assembled into crystalline mechanomorphogenic films, yielding self-healing materials that can change their macro-morphology in response to mechanical stimuli. These films exhibit responsive behavior to environmental factors and selective permeability, which has enabled the rapid extraction of perfluoroalkyl substances from contaminated water samples. Continued research in this area may lead to the design of adaptive molecular networks and living matter using interface-templated and fluorine-driven assembly phenomena.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Energy & Fuels
Yumeng Chen, Dian Zhang, Junguo Li, Changning Wu, Ke Liu
Summary: This study presents a case study on scavenging combustible matter in waste coal using a self-designed collector Ksw, consisting of kerosene, span, and water. The results showed that Ksw outperformed conventional collectors in terms of floatability and economic feasibility. The superior performance of Ksw was attributed not only to the oil droplet size and number but also to its specific composition features.
Review
Biotechnology & Applied Microbiology
Williams Olughu, Gurjot Deepika, Chris Hewitt, Chris Rielly
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Thomas R. J. Heathman, Alvin W. Nienow, Qasim A. Rafiq, Karen Coopman, Bo Kara, Christopher J. Hewitt
BIOCHEMICAL ENGINEERING JOURNAL
(2019)
Article
Engineering, Chemical
Halina Murasiewicz, Jesus Esteben
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2019)
Article
Biotechnology & Applied Microbiology
Elena Costariol, Marco Rotondi, Arman Amini, Christopher J. Hewitt, Alvin W. Nienow, Thomas R. J. Heathman, Martina Micheletti, Qasim A. Rafiq
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Review
Biotechnology & Applied Microbiology
P. Moutsatsou, J. Ochs, R. H. Schmitt, C. J. Hewitt, M. P. Hanga
BIOTECHNOLOGY LETTERS
(2019)
Article
Biotechnology & Applied Microbiology
Williams Olughu, Alvin Nienow, Chris Hewitt, Chris Rielly
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2020)
Article
Engineering, Multidisciplinary
Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzing
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Junaid Ali, Panagiota Moutsatsou, Fritz A. de la Raga, Christopher J. Hewitt, Alvin Nienow, Ivan Wall
BIOTECHNOLOGY AND BIOENGINEERING
(2020)
Article
Cell & Tissue Engineering
Soukaina Bahsoun, Karen Coopman, Elizabeth C. Akam
STEM CELL RESEARCH & THERAPY
(2020)
Article
Biotechnology & Applied Microbiology
Mariana Petronela Hanga, Fritz Anthony de la Raga, Panagiota Moutsatsou, Christopher J. Hewitt, Alvin W. Nienow, Ivan Wall
Summary: The study aimed to develop a scalable, microcarrier-based intensified bioprocess for expanding bovine adipose-derived stem cells, achieving a significant fold increase in cell expansion and maintaining cell quality pre- and post-expansion.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Mariana P. Hanga, Alvin W. Nienow, Halina Murasiewicz, Andrzej W. Pacek, Christopher J. Hewitt, Karen Coopman
Summary: The study demonstrated the use of perfluorocarbon liquid microcarriers for expanding and recovering human mesenchymal stem/stromal cells, showing that cells can successfully attach, proliferate, and maintain quality on these liquid carriers.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Halina Murasiewicz, Barbara Zakrzewska
CHEMICAL AND PROCESS ENGINEERING-INZYNIERIA CHEMICZNA I PROCESOWA
(2019)