Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 21, Issue 11, Pages 2796-2808Publisher
WILEY
DOI: 10.1111/jcmm.13194
Keywords
inflammation; apoptosis; ROS; NF-kappa B
Categories
Funding
- National Natural Science Foundation of China [31472254, 31272631]
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Staphylococcus aureus (S. aureus) causes severe inflammation in various infectious diseases, leading to high mortality. The clinical application of antibiotics has gained a significant curative effect. However, it has led to the emergence of various resistant bacteria. Therefore, in this study, we investigated the protective effect of polydatin (PD), a traditional Chinese medicine extract, on S. aureus lipoteichoic acid (LTA)-induced injury in vitro and in vivo. First, a significant improvement in the pathological conditions of PD in vivo was observed, suggesting that PD had a certain protective effect on LTA-induced injury in a mouse model. To further explore the underlying mechanisms of this protective effect of PD, LTA-induced murine macrophages were used in this study. The results have shown that PD could reduce the NF-kappa B p65, and IB phosphorylation levels increased by LTA, resulting in a decrease in the transcription of pro-inflammatory factors, such as TNF-alpha, IL-1 beta and IL-6. However, LTA can not only activate NF-kappa B through the recognition of TLR2 but also increase the level of intracellular reactive oxygen species (ROS), thereby activating NF-kappa B signalling. We also detected high levels of ROS that activate caspases 9 and 3 to induce apoptosis. In addition, using a specific NF-kappa B inhibitor that could attenuate apoptosis, namely NF-kappa B p65, acted as a pro-apoptotic transcription factor in LTA-induced murine macrophages. However, PD could inhibit the generation of ROS and NF-kappa B p65 activation, suggesting that PD suppressed LTA-induced injury by attenuating ROS generation and TLR2-NF kappa B signalling.
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