Review
Pharmacology & Pharmacy
Kai-Di Ni, Jun-Yan Liu
Summary: CYP omega-hydroxylases are a subfamily of enzymes that mediate the metabolism of fatty acids and their metabolites. The metabolites have pleiotropic effects in inflammation and related diseases. Further studies are needed to understand the function and mechanisms of other CYP omega-hydroxylases-mediated eicosanoids in order to develop therapeutic strategies for various diseases.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jingyan Zhang, Ying Sun, Yeji Wang, Xin Chen, Lu Xue, Jingjing Zhang, Xiangcheng Zhu, Yanwen Duan, Xiaohui Yan
Summary: Rubiginones are part of the angucycline family and have been shown to enhance the cytotoxicity of vincristine against drug-resistant cancer cell lines. Through genome mining of Streptomyces sp. CB02414, new rubiginones were discovered and a biosynthetic pathway was proposed based on the production profiles of wild-type and mutant strains. This research paves the way for further studies on cytochrome P450-catalyzed oxidation of angucyclines and the development of novel compounds through combinatorial biosynthesis strategies.
MICROBIAL CELL FACTORIES
(2021)
Review
Agriculture, Dairy & Animal Science
M. J. Kuhn, V Mavangira, L. M. Sordillo
Summary: Dairy cattle are at a high risk of disease around calving due to compromised immunity and oxidative stress, influenced by the metabolism of fatty acids and fat-soluble vitamins. The importance of the cytochrome P450 (CYP450) enzyme family in cattle health is underappreciated, with potential interactions between fatty acids and fat-soluble vitamins needing further exploration. Understanding the role of CYP450 in immune function and oxidant status could help in reducing postpartum disease occurrence.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Physiology
Melina Lopez, Pedro F. Malacarne, Deepak P. Ramanujam, Timothy Warwick, Niklas Mueller, Jiong Hu, Matthias Dewenter, Andreas Weigert, Stefan Guenther, Ralf Gilsbach, Stefan Engelhardt, Ralf P. Brandes, Flavia Rezende
Summary: The cytochrome P450 reductase (POR) plays an important role in the activity of microsomal cytochrome P450 enzymes, especially in cardiac endothelial cells. Loss of endothelial POR leads to cardiac remodeling and accelerated reduction in cardiac output under pressure overload conditions.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ling Wang, Xiaowei Wu, Chenghua Gao, Lingrui Wei, Qian Li, Aitao Li
Summary: A new cytochrome P450 enzyme, CYP68J5_fg, was identified from Fusarium graminearum, showing steroidal 12 beta- and 15 alpha-hydroxylase activities. This is the first fungal P450 enzyme with 12 beta-hydroxylase activity discovered, offering opportunities for enhancing efficiency and selectivity in steroid drug synthesis.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Shuai Zhang, Song Gao, Yu Chen, Sha Xu, Shiqin Yu, Jingwen Zhou
Summary: The leaves of Ampelopsis grossedentata are rich in dihydromyricetin, which has many potential applications. In this study, hydroxylase genes involved in the biosynthesis of dihydromyricetin were identified and characterized. The hydroxylation process and carbon flux pathways of dihydromyricetin in Ampelopsis grossedentata were successfully identified.
Review
Biochemistry & Molecular Biology
Maria Norlin, Kjell Wikvall
Summary: Vitamin D signaling plays a crucial role in maintaining calcium balance for bone health and has various functions in different tissues. Disturbances in vitamin D signaling are associated with numerous diseases. This review focuses on the identification of bioactivating enzymes and their genes involved in the production of active vitamin D metabolites. It evaluates species- and tissue-specific expression, enzyme kinetics, substrate specificity, and the consequences of gene mutations. The roles of different vitamin D receptors and alternative bioactivation pathways are also discussed. Although significant progress has been made in understanding vitamin D bioactivation enzymes, further research is needed to fully comprehend the pleiotropic effects and the mechanisms of enzymatic activation in vitamin D-induced responses.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Ragini D. D. Singh, Avadhesh Avadhesh, Gaurav Sharma, Sagar Dholariya, Rima B. B. Shah, Bela Goyal, Subash Chandra Gupta
Summary: Targeted cancer therapy using CYP as drug target has shown promise, but there are still challenges including drug resistance and limited therapeutic index. Recent advances in molecular tools and structure-activity relationship understanding have helped in the development of CYP-based drugs with better efficacy. Future directions involve the development of more selective tools to understand molecular alterations and develop specific CYP inhibitors/inducers.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Kathleen Lukaszewicz, John R. R. Falck, Julian Lombard
Summary: In Dahl salt-sensitive rats, impaired vascular relaxation can be restored by restoring physiological levels of angiotensin II, inhibiting 20-HETE production, or introducing a normally functioning renin allele. However, chronically low angiotensin II levels in SS rats were found to upregulate cytochrome P450-4A and increase the production of the vasoconstrictor 20-HETE. The renin-angiotensin system and the CYP4A/20-HETE pathway both contribute to vascular dysfunction in the Dahl SS rat, but they are independent of each other.
Article
Biochemistry & Molecular Biology
Taisei Yamamoto, Yoshie Hasegawa, Hiroaki Iwaki
Summary: Cytochrome P450 monooxygenases are important in metabolism. In this study, a novel self-sufficient cytochrome P450, P450(CHC), was identified and characterized in Paraburkholderia terrae KU-64, which degrades cyclohexanecarboxylate.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Zihan Zhang, Qing-Yang Wu, Yue Ge, Zheng-Yu Huang, Ran Hong, Aitao Li, Jian-He Xu, Hui-Lei Yu
Summary: This review article highlights the research progress in the identification, molecular modification, and functional expression of hydroxylases in the past decade, which are beneficial for the discovery, engineering, and application of more hydroxylases involved in plant secondary metabolism.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Cell Biology
Wenxing Cui, Xun Wu, Yingwu Shi, Wei Guo, Jianing Luo, Haixiao Liu, Longlong Zheng, Yong Du, Ping Wang, Qiang Wang, Dayun Feng, Shunnan Ge, Yan Qu
Summary: The study found that the inhibition of 20-HETE synthesis reduced neuronal apoptosis, reactive oxygen species production, and damage to mitochondrial structures after traumatic brain injury (TBI). Mechanistically, the inhibitor also regulated mitochondrial dynamics and promoted the restoration of SIRT1 and PGC-1 alpha, which are crucial in cell function and survival. Moreover, plasma 20-HETE levels were correlated with the neurological outcomes in TBI patients.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Laurentijn Tilleman, Kaat Rubben, Wim Van Criekinge, Dieter Deforce, Filip Van Nieuwerburgh
Summary: This study tested the effectiveness of Targeted Locus Amplification (TLA) combined with Illumina or Nanopore sequencing in accurate genotyping and phasing of pharmacogenes. The results showed limited nucleotide mapping on target and incomplete coverage of genes with both sequencing methods. However, the optimized primer pair for the CYP2D6 gene in the Nanopore dataset resulted in correct haplotypes, indicating the potential reliability of this method with patient-specific primer design and optimization.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Liyuan Hou, Erica L. -W. Majumder
Summary: This study identified microbial species capable of degrading PS and predicted target PS-degrading enzymes. Enzymes such as cytochrome P4500s, alkane hydroxylases, and monooxygenases are considered top potential candidates for degrading PS, with the potential to break C-C bonds and oxidize aromatic compounds generated from PS decomposition. These enzymes are distributed in various bacterial phyla, suggesting broad potential for PS biodegradation in different environments.
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Tiecheng Zhong, Zonggui Wang, Sayeman Islam Niloy, Yue Shen, Stephen T. O'Rourke, Chengwen Sun
Summary: Cardiac hypertrophy is initially an adaptive response to cardiac overload, but can progress to decompensation, leading to heart failure and sudden cardiac death. This study found that both PI3KC1 and PI3KC3 are involved in Ang II-induced cardiac hypertrophy, with PI3KC1 inhibiting autophagy activity leading to ROS accumulation and hypertrophy, while PI3KC3 activation improving autophagy activity, reducing ROS production, and providing a protective effect against Ang II-induced cardiac hypertrophy.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Amreen Mughal, Chengwen Sun, Stephen T. O'Rourke
Summary: The study indicates that apelin does not inhibit BKCa channel function in coronary arteries, and therefore does not interfere with NO-dependent relaxation in coronary arteries.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Engineering, Aerospace
Hongbo Guo, Xiongbin Jia, Ningbo Zhao, Shuying Li, Hongtao Zheng, Chengwen Sun, Xiang Chen
Summary: This study investigates the formation and development characteristics of oblique detonation waves in stoichiometric hydrogen-oxygen mixtures diluted with argon using different chemical kinetic processes. The results show significant differences in the flow field structure of oblique detonation waves under 19-step and 34-step mechanisms, with H2O2 playing a key role in triggering and accelerating the exothermic process. The properties of H, O, and H2O2 radicals vary in different chemical reaction models.
AEROSPACE SCIENCE AND TECHNOLOGY
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Sayeman Islam Niloy, Yagna Jarajapu, Stephen O'Rourke, Chengwen Sun
Meeting Abstract
Biochemistry & Molecular Biology
Santo K. Anto, Chengwen Sun, Stephen T. O'Rourke
Article
Cardiac & Cardiovascular Systems
Santo Anto, Venkatachalem Sathish, Chengwen Sun, Stephen T. O'Rourke
Summary: This study found that exposure to cigarette smoke extract (CSE) impairs the vasorelaxant effect of apelin in coronary arteries and reduces its ability to inhibit endothelium-dependent relaxation to acetylcholine (ACh).
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Cheng-Wen Sun, Bojan Obrenovic, Hai-Ting Li
Summary: The study found that virtual CSR co-creation can promote customers' willingness to purchase green products. Virtual CSR co-creation and self-construal have an interactive effect on green product purchase intention, and environmental involvement plays a moderating role in this interaction. Virtual community identity partially mediates this interactive effect.
Review
Biochemistry & Molecular Biology
Richard Nii Lante Lamptey, Chengwen Sun, Buddhadev Layek, Jagdish Singh
Summary: Hypertension is a global health concern, and neurogenic hypertension (NH) accounts for almost half of all hypertension cases. The increased sympathetic nervous system activity mediated by angiotensin II in the brain is known to be the main cause of NH. However, traditional antihypertensive drugs lack the ability to effectively penetrate the blood-brain barrier, making it challenging to manage NH. Therefore, developing strategies for targeted delivery of antihypertensives to the brain is crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Urology & Nephrology
Chengwen Sun, Dongrong Yang, Jin Zhu, Yibin Zhou, Congming Xiang, Sheng Wu
Summary: This study suggests that the modified staging system may be more effective in predicting prognosis for patients with advanced prostate cancer compared to the AJCC 8th staging system, indicating a need for further optimization.
Article
Pharmacology & Pharmacy
Richard Nii Lante Lamptey, Chengwen Sun, Jagdish Singh
Summary: Functionalized liposomes can safely deliver losartan potassium to the brain, blocking angiotensin receptor type 1 activity and preventing the occurrence of hypertension in spontaneously hypertensive rats.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Materials Science, Multidisciplinary
Weicheng Kong, Detao Liu, Chengwen Sun, Dejun Kong
Summary: A CuCMnNiTi coating was prepared on Ti6Al4V alloy using laser cladding technique. The coating's corrosion resistance was improved by tempering at 450 and 500℃.
MATERIALS CHARACTERIZATION
(2023)
Article
Chemistry, Medicinal
Qi Zhang, Yue Shen, Sayeman Islam Niloy, Stephen T. O'Rourke, Chengwen Sun
Summary: This study found that chronic infusion of apelin-13 does not change blood pressure or heart rate, and does not alter Ang II-induced hypertension and cardiac hypertrophy. These findings suggest that an APJ receptor biased agonist could be a better therapeutic alternative for treatment of hypertension.
Article
Biochemistry & Molecular Biology
Fanrong Yao, Sayeman Islam Niloy, Yue Shen, Qi Zhang, Stephen T. O'Rourke, Chengwen Sun
Summary: Intravenous administration of apelin-13 causes a significant decrease in blood pressure, while not affecting heart rate and contractility. Furthermore, the plasma from apelin-treated rats induces relaxation of blood vessels through a NO-independent mechanism involving an unidentified substance.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Sayeman Islam Niloy, Yue Shen, Lirong Guo, Stephen T. O'Rourke, Chengwen Sun
Summary: This study reveals that dysfunction of BKCa channels in vascular smooth muscle cells (VSMCs) is related to abnormal proliferation and morphological changes in hypertension, possibly due to decoupling of IP3R-BKCa. Genetic disruption of IP3R-BKCa coupling increases VSMC proliferation, while infusion of the BKCa opener NS1619 attenuates vascular hypertrophy in hypertensive rats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cheng Ying, Sun Cheng-Wen, Qin Yan, Shi Shuai, Li Yue-Yang, Fan Qi-Gao, Yang Gang-Long, Gao Xiao-Dong
Summary: This study developed an experimental procedure for in situ extraction of N-linked glycans based on FFPE tissue sections, and found abnormal N-linked glycans modification in bladder cancer tissues. Some N-linked glycans showed significantly increased relative intensity in bladder cancer tissues, while others showed significantly decreased relative intensity. These different N-linked glycans may serve as potential biomarkers for bladder cancer.
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
(2022)