Journal
DALTON TRANSACTIONS
Volume 44, Issue 11, Pages 4918-4924Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4dt03115f
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- Lyon Science Transfer Agency
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During the course of our research studies aiming to combine the unique photophysical properties of lanthanide complexes and the intrinsic advantage of a nonlinear two-photon excitation, we described the proof-of-concept of a biphotonic multiplexing bio-imaging experiment. To that end, a new Tb-luminescent bioprobe [TbL2] was designed using the TACN-trispicolinate platform. The biphotonic imaging of T24 fixed cells stained either with [TbL2] or with the already described [EuL1] complexes is reported. Finally the biphotonic multiplexing experiment was performed using a combination of both probes using the spectral mode of the two-photon microscope.
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