Article
Oncology
Diyi Zhou, Lijun Chen, Xin Mou
Summary: Acarbose can promote the proliferation of islet beta cells and inhibit PDX-1 methylation in diabetic mice, providing a new strategy for treating T2DM.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Jiarong Li, Hua Zhang, Hui Ouyang, Weixin Xu, Yong Sun, Youbao Zhong, Lifang Wang, Jiaxing Huang, Junchang Chen, Mingyao Li, Weifeng Zhu, Yuhui Liu, Ronghua Liu
Summary: This study evaluated the effects of Pueraria Thomsonii Radix water extract (PTR) on type 2 diabetes mellitus (T2DM) and found that PTR could improve T2DM symptoms, metabolic disorders, and gut microbiota imbalance in db/db mice. The study also suggested that gamma-aminobutyric acid (GABA) may be involved in the regulation of the microbiota-gut-brain axis (MGB) and affect the metabolic disorders associated with T2DM. These findings provide a scientific basis for the development of functional food with PTR.
Article
Physiology
Swayam Prakash Srivastava, Julie E. Goodwin
Summary: This study shows that the absence of endothelial glucocorticoid receptor (GR) leads to accelerated organ fibrosis in a mouse model of diabetes, through upregulation of Wnt signaling. Additionally, the study demonstrates that the fibrosis can be improved with a Wnt inhibitor or metformin. The findings highlight the importance of the synergistic effects of Wnt signaling and inflammation in the pathogenesis of organ fibrosis.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Nutrition & Dietetics
Shujuan Zheng, Yanan Wang, Jingjing Fang, Ruixuan Geng, Mengjie Li, Yuhan Zhao, Seong-Gook Kang, Kunlun Huang, Tao Tong
Summary: The study found that oleuropein may ameliorate the advanced stage of type 2 diabetes through modulating the composition and function of gut microbiota, providing a promising therapeutic approach for the treatment of advanced stage type 2 diabetes.
Article
Biochemistry & Molecular Biology
Oelisoa M. Andriankaja, Reuben Adatorwovor, Alpdogan Kantarci, Hatice Hasturk, Luciana Shaddox, Michael A. Levine
Summary: This study assessed the association between serum or gingival crevicular fluid endothelial and inflammatory mediators and chronic periodontal disease among Hispanic adults with type 2 diabetes. The findings showed that oral and systemic endothelial and inflammatory mediators are related to periodontal clinical parameters.
Article
Biochemistry & Molecular Biology
Xiaoxuan Song, Zeyu Zhu, Xiaohang Qian, Xiaoli Liu, Shengdi Chen, Huidong Tang
Summary: This study investigated the potential mechanisms and interventions underlying T2DM-induced cognitive deficits by analyzing the brain transcriptome, plasma metabolome, and gut microbiota in db/db mice. The results suggest that disturbances in mitochondrial respiratory, glycolytic, and inflammatory pathways, as well as altered glucose, lipid, bile acid, and steroid metabolism, may contribute to cognitive impairment in these mice. The study also provides novel insights into the functional interactions among the brain, circulating metabolites, and gut microbiota.
Article
Multidisciplinary Sciences
Kanae Mukai, Shin-ichi Horike, Makiko Meguro-Horike, Yukari Nakajima, Arya Iswara, Toshio Nakatani
Summary: Topical estrogen application can enhance cutaneous wound healing in female mice with type 2 diabetes by reducing the wound area, promoting re-epithelialization and angiogenesis, and increasing the number of M2 macrophages.
Article
Multidisciplinary Sciences
Arya Iswara, Kenta Tanaka, Tatsuo Ishijima, Yukari Nakajima, Kanae Mukai, Yasunori Tanaka, Yusuke Nakano, Junko Sugama, Makoto Oe, Mayumi Okuwa, Toshio Nakatani
Summary: This study investigated the effects of non-thermal plasma treatment under non-contact conditions on the healing of full-thickness wounds in diabetic mice. The results showed that non-thermal plasma treatment reduced exudate production, promoted wound healing, and increased the formation of new blood vessels.
Article
Endocrinology & Metabolism
Hongfang Zeng, Xiaoli Li, Duanfang Zhou, Ning Wang, Xiaoping Yu, Liangyuan Long, Hao Cheng, Shuyu Zhou, Zhengze Shen, Weiying Zhou
Summary: Qihu has shown anti-diabetic effects in db/db mice by lowering blood glucose levels, reducing bodyweight gain, and downregulating inflammatory factors through the activation of the AMPK/Txnip signaling pathway.
DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Ruoyun Wu, Lina Zhou, Yan Chen, Xiaoqin Ding, Yan Liu, Bei Tong, Han Lv, Xiuhua Meng, Jing Li, Tunyu Jian, Jian Chen
Summary: Loquat leaf contains sesquiterpene glycosides that can prevent type 2 diabetes by relieving insulin resistance and inflammation, as well as reshaping the gut microbiota.
Article
Cell Biology
Cristian De Gregorio, Fernando Ezquer
Summary: Diabetic neuropathy (DN) is a common complication of diabetes mellitus (DM) leading to chronic pain, sensory loss, and muscle atrophy. Neurons derived from diabetic mice exhibit regeneration defects and reduced responses to neurotrophic factors compared to non-diabetic mice. Treatment with mesenchymal stem cell secretome improves neuritic regeneration in diabetic neurons but remains reduced compared to non-diabetic neurons.
DISEASE MODELS & MECHANISMS
(2021)
Article
Cardiac & Cardiovascular Systems
Loryn J. Bohne, Hailey J. Jansen, Irene Daniel, Tristan W. Dorey, Motahareh Moghtadaei, Darrell D. Belke, Martin Ezeani, Robert A. Rose
Summary: The study revealed that AF susceptibility in db/db mice is linked to impaired electrical conduction, along with electrical and structural remodeling of the atria.
Article
Biochemistry & Molecular Biology
Gustav Dallner, Magnus Bentinger, Shafaat Hussain, Indranil Sinha, Jiangning Yang, Cheng Schwank-Xu, Xiaowei Zheng, Ewa Swiezewska, Kerstin Brismar, Ismael Valladolid-Acebes, Michael Tekle
Summary: Deh-T3 beta has multifaceted capacities in improving diabetes complications, including enhancing wound healing, improving kidney and liver functions, reducing liver steatosis, and enhancing heart recovery after ischemia. The compound exerts these positive effects in several organs of diabetic mice without decreasing non-fasting blood glucose levels, suggesting its antioxidant properties and improvement in mitochondrial function play a central role in reducing diabetes complications.
Article
Endocrinology & Metabolism
Nathaniel L. Baker, Samar M. Hammad, Kelly J. Hunt, Andrea Semler, Richard L. Klein, Maria F. Lopes-Virella
Summary: This study found that plasma apoM levels are associated with the development of diabetic kidney disease. Higher apoM levels are associated with increased risk of progression to macroalbuminuria and chronic kidney disease in patients with type 1 diabetes, suggesting that alterations in apoM may play a role in the development of nephropathy.
Article
Immunology
Wenqi Fan, Haipeng Pang, Xiajie Shi, Jiaqi Li, Yimeng Wang, Shuoming Luo, Jian Lin, Haibo Yu, Yang Xiao, Xia Li, Gan Huang, Zhiguo Xie, Zhiguang Zhou
Summary: This study identified the plasma-derived exosomal mRNA expression profiles of T1DM for the first time, suggesting that the identified DEMs may play a role in the pathogenesis of T1DM and have the potential to serve as biomarkers and therapeutic targets for T1DM.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Instruments & Instrumentation
Karolina Augustyniak, Karolina Chrabaszcz, Marta Smeda, Marta Stojak, Katarzyna M. Marzec, Kamilla Malek
Summary: Label-free molecular imaging, particularly with higher magnification in FT-IR microscopy, can provide detailed insights into lung structures and histopathological features, allowing for better evaluation of chemical composition in disease models.
APPLIED SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Marek Grosicki, Jakub Dybas, Ewelina Matuszyk, Marko Rodewald, Tobias Meyer-Zedler, Michael Schmitt, Juergen Popp, Kamilla Malek, Malgorzata Baranska
Summary: The study compared the response to inflammation between isolated human eosinophils and EoL-1 cells in terms of lipid bodies (LBs) using fluorescence, Raman, and coherent anti-Stokes Raman scattering (CARS) microscopy. Differences were observed in the number and chemical composition of LBs, indicating varying manifestations of inflammatory processes depending on the cell type and stimulation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Ewelina Wiercigroch, Pawel Swit, Agnieszka Brzozka, Lukasz Pieta, Kamilla Malek
Summary: In this study, a novel SERS immunoassay is demonstrated, operating in a dual surface enhancement and dual-tag paradigm, which improves the detection sensitivity and reliability.
Article
Spectroscopy
Prasad L. Polavarapu, Jordan L. Johnson, Vijay Raghavan, Grzegorz Zajac, Malgorzata Baranska
Summary: Experimental vibrational Raman and Raman optical activity (ROA) spectra were measured for diacetyl L-tartaric acid (DAT), its esters T1OH and T12OH, and corresponding sodium salts DATNa, T1ONa, and T12ONa. Quantum chemical (QC) predictions using the B3LYP functional and 6-311++G(2d,2p) basis set were used to interpret the ROA spectra. Implicit solvation model (PCM) was inadequate for predicting the ROA spectra of DAT and T1OH in aqueous solutions. However, it satisfactorily predicted the ROA spectra for DATNa and T1ONa. The overwhelming number of conformations for T12OH and T12ONa hindered QC predictions, but predictions made for T1OH and T1ONa can be applied to explain the experimental ROA spectra of T12OH and T12ONa. The study highlights the importance of converting carboxylic acids to sodium salts and QC predictions for shorter alkyl chain analogs in interpreting the ROA spectra of chiral surfactants.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Monika Soltys-Mroz, Karolina Syrek, Lukasz Pieta, Kamilla Malek, Grzegorz D. Sulka
Summary: In this study, Fe2O3-TiO2 materials were synthesized by cathodic electrochemical deposition, and the effect of hematite content on the photoelectrochemical activity of the materials was investigated. The results showed that the amount of deposited iron and hematite content increased with deposition time, directly impacting the semiconducting properties and enhancing the photoelectrochemical water oxidation and visible light response of the materials.
Article
Biophysics
Basseem Radwan, Stefano Rocchetti, Ewelina Matuszyk, Magdalena Sternak, Maciej Stodulski, Robert Pawlowski, Jacek Mlynarski, Krzysztof Brzozowski, Stefan Chlopicki, Malgorzata Baranska
Summary: This study introduces a Raman-based imaging method using EdU as a probe to track endothelial cell proliferation, providing an alternative to fluorescence-based assays for assessing endothelial proliferation and regeneration.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Monika Kujdowicz, David Perez-Guaita, Piotr Chlosta, Krzysztof Okon, Kamilla Malek
Summary: A novel approach combining infrared microscopy and machine learning was proposed to automatically predict the presence of cancer, invasiveness, and grade in urothelial bladder carcinoma (BC). The study found that the infrared imaging showed high sensitivity in identifying cancer, invasiveness, and grade, providing a potential ancillary platform for screening bladder biopsies.
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Anna Pieczara, Krzysztof Czamara, Marta Stojak, Ewelina Matuszyk, Katarzyna Majzner, Krzysztof Brzozowski, Arianna Bresci, Dario Polli, Malgorzata Baranska
Summary: In this study, the functionality of inflamed cells was verified by analyzing their ability to uptake and accumulate exogenous saturated fatty acids. The results showed that the uptake of fatty acids in inflamed cells is dependent on their concentration and type, providing a better understanding of endothelial cell inflammation and its impact on their fat buffering capacity. The microscopic methods used in the analysis showed complementarity in studying fatty acid uptake by endothelial cells from different perspectives.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biophysics
Anna Pieczara, Aleksandra Borek-Dorosz, Szymon Buda, William Tipping, Duncan Graham, Robert Pawlowski, Jacek Mlynarski, Malgorzata Baranska
Summary: This study uses a Raman probe, 3-OPG, to track metabolic changes in endothelial cells. The results show that 3-OPG is a sensitive sensor for studying glucose metabolism and its changes in cellular inflammation. This research is important for understanding the role of endothelial cell metabolism in disease development.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Karolina Augustyniak, Aleksandra Pragnaca, Monika Lesniak, Marta Halasa, Agata Borkowska, Ewa Pieta, Wojciech M. Kwiatek, Claudine Kieda, Robert Zdanowski, Kamilla Malek
Summary: Circulating endothelial cell progenitors from the bone marrow have potential in repairing endothelium damage. This study used label-free Raman and FTIR imaging to distinguish between brain endothelium and endothelial progenitor cells in mice. The results showed that lipid droplets play a key role in differentiating these two cell types.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Spectroscopy
Vikas Kumar, Till Reichenauer, Luca Supovec, Dennis Jansen, Nikolai Brodt, Grzegorz Zajac, Agnieszka Domagala, Malgorzata Baranska, Jochen Niemeyer, Sebastian Schluecker
Summary: CARS-ROA is a coherent technique for chirally sensitive vibrational spectroscopy, which has the advantage of significantly reducing acquisition times compared to traditional ROA. In this study, the first demonstration of heterodyne-detected CARS-ROA of chiral molecules in an achiral solvent was achieved using a conventional broadband CARS spectrometer. This work is a crucial step towards kinetic reaction monitoring in organocatalysis with short acquisition times.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Basseem Radwan, Amrutha Prabhakaran, Stefano Rocchetti, Ewelina Matuszyk, Tia E. Keyes, Malgorzata Baranska
Summary: The cellular uptake and bioavailability of anti-inflammatory antioxidant astaxanthin (AXT) can be improved via liposomal encapsulation. Endothelial cells (EC), which line blood vessels, play important roles in maintaining cardiovascular homeostasis. Dysfunction of EC is associated with various diseases and is closely related to oxidative stress and inflammation. Liposomal encapsulation of AXT showed enhanced uptake and anti-inflammatory effects, especially in reducing lipid unsaturation, lipid droplets, and intercellular adhesion molecule 1 (ICAM-1) overexpression in EC.
Review
Chemistry, Analytical
Adriana Adamczyk, Sylwia Orzechowska, Anna Maria Nowakowska, Krzysztof Brzozowski, Katarzyna Majzner, Malgorzata Baranska
Summary: This article presents the recent advances in using stimulated Raman scattering (SRS) microscopy for spectroscopic analysis of cancer cells to identify pathological changes and understand the mechanism of metastasis. SRS microscopy, with its stronger signals and ability to time-resolve vibrational motions, offers rapid analysis of single cells. The article showcases the development of modern Raman imaging technology based on nonlinear optics and its application in analyzing various tumor cells, particularly leukemia.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ewa Machalska, Grzegorz Zajac, Malgorzata Baranska, Petr Bour, Dorota Kaczorek, Robert Kawecki, Joanna E. Rode, Krzysztof Lyczko, Jan Cz Dobrowolski
Summary: In this study, we discovered that the recently discovered ECD-Raman effect is dominant over natural Raman optical activity in atropisomeric naphthalenediimides. We investigated the type of molecular structure information that can be obtained from the spectra. We found that the spectra depended significantly on solute substitution and/or solvent change. Furthermore, the spectra could be accurately predicted by theory, providing an interesting tool for monitoring the chirality of binaphthyl compounds.
CHEMICAL COMMUNICATIONS
(2022)