Spectroscopic investigation on interaction of biogenic, Croton bonplandianum leaves extract mediated potential bactericidal silver nanoparticles with human hemoglobin and human serum albumin

The green synthesis of nanoparticles has received increasing attention due to the growing demand to produce safe, cost-effective, and eco-friendly technology for nanomaterials synthesis. We report on the use of aqueous Croton bonplandianum (Family: Euphorbiaceae, Genus: Croton) leaves extract for the preparation of silver nanoparticles (AgNPs) without using any external reducing and stabilizing agent. Ultraviolet-visible spectroscopy showed maximum absorbance at 446nm due to surface plasmon resonance of AgNPs. Energy dispersive X-ray spectra also supported the existence of AgNPs. An average diameter (d=17.4nm) of the spherical AgNPs was determined from the transmission electron microscopic images. Hydrodynamic size (d=21.1nm) was determined by dynamic light scattering. Fourier transform infrared analysis designed that the functional groups like O-H, N-H, CONH2, and COOH participated in the AgNPs formation. The negative zeta potential value (-19.3mV) of the AgNPs indicated its dispersion and stability. The AgNPs exhibited strong antibacterial activity against Escherichia coli ATCC 25922 and 1.5nM proved to be minimum inhibitory concentration for it. Hemolysis assay demonstrated the blood compatibility of the AgNPs toward human RBCs. The binding affinity of the AgNPs toward human hemoglobin and human serum albumin (HSA) was also determined by means of fluorescence spectroscopy. The circular dichroism spectroscopy revealed that the native structures of human hemoglobin and HSA remain unchanged, but its secondary structures were slightly changed upon interaction with AgNPs. Overall, it can be concluded that the AgNPs may be applied in the area of nanomedicines.