Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 105, Issue 8, Pages 2182-2190Publisher
WILEY
DOI: 10.1002/jbm.a.36075
Keywords
biodegradable polymers; carbon nanotubes; graphene nanoplatelets; poly(lactic) acid; anterior cruciate ligament
Funding
- Portuguese Foundation for Science and Technology (FCT) [PTDC/EME-PME/114808/2009, UID/BIM/04293/2013]
- NORTE2020 Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000012]
- FCT [SFRH/BD/78749/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/78749/2011, PTDC/EME-PME/114808/2009] Funding Source: FCT
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Anterior cruciate ligament (ACL) reconstructive surgeries are the most frequent orthopedic procedures in the knee. Currently, existing strategies fail in completely restoring tissue functionality and have a high failure rate associated, presenting a compelling argument towards the development of novel materials envisioning ACL reinforcement. Tendons and ligaments, in general, have a strong demand in terms of biomechanical features of developed constructs. We have previously developed polylactic acid (PLA)-based biodegradable films reinforced either with graphene nanoplatelets (PLA/GNP) or with carboxylfunctionalized carbon nanotubes (PLA/CNT-COOH). In the present study, we comparatively assessed the biological performance of PLA, PLA/GNP, and PLA/CNT-COOH by seeding human dermal fibroblasts (HFF-1) and studying cell viability and proliferation. In vivo tests were also performed by subcutaneous implantation in 6-week-old C57Bl/6 mice. Results showed that all formulations studied herein did not elicit cytotoxic responses in seeded HFF-1, supporting cell proliferation up to 3 days in culture. Moreover, animal studies indicated no physiological signs of severe inflammatory response after 1 and 2 weeks after implantation. Taken together, our results present a preliminary assessment on the compatibility of PLA reinforced with GNP and CNT-COOH nanofillers, highlighting the potential use of these carbon-based nanofillers for the fabrication of reinforced synthetic polymerbased structures for ACL reinforcement. (C) 2017 Wiley Periodicals, Inc.
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