Article
Multidisciplinary Sciences
John K. Barrows, Baicheng Lin, Colleen E. Quaas, George Fullbright, Elizabeth N. Wallace, David T. Long
Summary: BRD4 is a multifunctional regulator of chromatin binding that plays a direct role in DNA double-strand break repair, promoting DNA processing through interactions with the SWI/SNF chromatin remodeling complex and resection machinery.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Kevin Bloh, Natalia Rivera-Torres
Summary: The mechanism of ssODN-directed gene editing has been a topic of discussion within the field of CRISPR gene editing. The ExACT pathway, based on oligo-driven DNA repair, provides a comprehensive understanding of the different ways DNA repair can occur in the presence of a repair oligonucleotide after CRISPR cleavage, by comparing and challenging other similar DNA repair pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Biochemistry & Molecular Biology
Brett M. Sansbury, Eric B. Kmiec
Summary: The expectations for selectively modifying the human genome have never been higher in the last five years. The development of site-specific nucleases is centerstage in effective molecular therapies, with a focus on correcting genetic mutations that cause cancer and inherited diseases. Homology directed repair, a process for reversing mutations and restoring normal protein function, is a key technique that is being researched for human gene editing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Theodore Busby, Yuechuan Chen, Tanner C. Godfrey, Mohammad Rehan, Benjamin J. Wildman, Caris M. Smith, Quamarul Hassan
Summary: This article investigates tissue-specific regulation in mineralized tissues through chromatin remodeling, focusing on the expression of Baf45a and Baf45d. The study finds that Baf45a and Baf45d are preferentially expressed in osteoblasts and odontoblasts, and their expression is associated with the differentiation and maturation of these cells. The research also reveals the importance of the interaction between BAF45A, PBAF subunits, and the transcription factor RUNX2 in regulating gene expression in mineralized tissues.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Immunology
Shmuel Avlas, Hala Kassis, Michal Itan, Hadar Reichman, Avishay Dolitzky, Inbal Hazut, Sharon Grisaru-Tal, Yaara Gordon, Ilan Tsarfaty, Danielle Karo-Atar, Perri Rozenberg, Almog Bitton, Ariel Munitz
Summary: Chronic inflammation is a characteristic feature of various inflammatory diseases, including inflammatory bowel disease (IBD). CD300 receptor family members have been shown to play important roles in innate immunity, as well as in allergic and autoimmune diseases. CD300b has been found to have a central and distinct role in colonic inflammation and subsequent repair, and it may be a future therapeutic target in inflammatory gastrointestinal (GI) diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Courtney Chambers, Katerina Cermakova, Yuen San Chan, Kristen Kurtz, Katharina Wohlan, Andrew Henry Lewis, Christiana Wang, Anh Pham, Milan Dejmek, Michal Sala, Mario Loeza Cabrera, Rogelio Aguilar, Radim Nencka, H. Daniel Lacorazza, Rachel E. Rau, H. Courtney Hodges
Summary: In acute myeloid leukemia (AML), SWI/SNF chromatin remodeling complexes sustain leukemic identity by driving high levels of MYC. PU.1 binds to most of its targets in a SWI/SNF-independent manner and recruits SWI/SNF to promote accessibility for other AML core regulatory factors. SWI/SNF inhibition induces therapeutic response in AML but also impairs PU.1-dependent B-cell and monocyte populations, revealing the on-and off-tumor effects of SWI/SNF blockade.
Article
Biochemistry & Molecular Biology
Guoling Li, Xiaohui Yang, Xinxin Luo, Zhenfang Wu, Huaqiang Yang
Summary: The use of small molecules to synchronize the cell cycle in S and G2/M phases enhances the efficiency of CRISPR/Cas9-mediated homology-directed repair (HDR) in animal cells and embryos. The study reveals the common molecular mechanisms that connect cell cycle progression and HDR activity.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jirong Wang, Chengyun Xu, Jing Zhang, Yizhong Bao, Ying Tang, Xiaoling Lv, Bo Ma, Ximei Wu, Genxiang Mao
Summary: RhoA protein plays a crucial role in regulating the cytoskeletal architecture of osteoclasts and bone resorption activity. Deletion of RhoA leads to severe osteopetrosis phenotype and suppression of bone resorption, which is mediated by inhibiting Akt-mTOR-NFATc1 signaling during osteoclast differentiation. Activation of RhoA is associated with enhanced osteoclast activity and the development of osteoporotic bone phenotype. The absence of RhoA in osteoclast precursors in mice prevents OVX-induced bone loss.
MOLECULAR MEDICINE
(2023)
Article
Cell Biology
Li Yang, Xin Wang, Xin-Xin Yu, Lu Yang, Bi-Chen Zhou, Jing Yang, Cheng-Ran Xu
Summary: The study reveals that promoter-associated histone modifications and chromatin accessibility dynamics effectively distinguish the default and directed processes of hepatoblast differentiation. The presence of H3K27me3 on bivalent promoters is associated with asymmetric differentiation strategy. The research also highlights the role of enhancer-associated modifications in the progressive development of hepatobiliary lineages.
DEVELOPMENTAL CELL
(2023)
Review
Pharmacology & Pharmacy
Adrian B. C. Lee, Meng-How Tan, Christina L. L. Chai
Summary: Using small molecules to increase the efficiency of HDR and decrease NHEJ to improve the efficiency of precise knock-in genome editing.
DRUG DISCOVERY TODAY
(2022)
Article
Cardiac & Cardiovascular Systems
Raja Chakraborty, Allison C. Ostriker, Yi Xie, Jui M. Dave, Ana Gamez-Mendez, Payel Chatterjee, Yaw Abu, Jake Valentine, Kimberly Lezon-Geyda, Daniel M. Greif, Vincent P. Schulz, Patrick G. Gallagher, William C. Sessa, John Hwa, Kathleen A. Martin
Summary: This study reveals that p300 and CBP play distinct and opposing roles in VSMC phenotypic switching and co-regulate chromatin modifications through different functional interactions with TET2 or HDACs. Targeting specific histone acetyl-transferases may hold therapeutic promise for cardiovascular diseases.
Article
Multidisciplinary Sciences
Chao Yang, Zhenzhen Ma, Keshan Wang, Xingxiao Dong, Meiyu Huang, Yaqiu Li, Xiagu Zhu, Ju Li, Zhihui Cheng, Changhao Bi, Xueli Zhang
Summary: Limited research has been conducted on concurrent C-to-G and A-to-G base editing. The authors show that HMGN1 enhances the efficiency of base editing and develop a CRISPR-based dual-function A-to-G and C-to-G base editor (GGBE) by fusing HMGN1 to GBE and ABE.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Guoling Li, Haoqiang Wang, Xianwei Zhang, Zhenfang Wu, Huaqiang Yang
Summary: This study presents a strategy to enhance HDR efficiency in animal cells by spatial and temporal colocalization of the donor and Cas9 through coupling the CRISPR system with the transcription factor THAP11. By appending THAP11-specific DNA binding motifs to the double-stranded donor DNA, the HDR efficiency can be significantly improved. The THAP11-fused CRISPR system achieved a more than twofold increase in HDR-mediated knock-in efficiency, and up to 6-fold increase can be achieved through combinational use with the small-molecule HDR enhancer valnemulin.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Genetics & Heredity
Judit Jimenez-Sainz, Adam Krysztofiak, Jennifer Garbarino, Faye Rogers, Ryan B. Jensen
Summary: This study reveals that the BRCA2 R3052W variant impairs the DNA damage response function of BRCA2 and leads to genome instability and defective DNA repair.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Pu Xu, Bin Deng, Bingyu Zhang, Qing Luo, Guanbin Song
Summary: The study implicates the mechanosensitive gene Tnmd in the regulation of tenocyte migration, with its expression being regulated by mechanical loading and actin stress fiber formation as well as chromatin decondensation. This novel role of Tnmd in tenocyte migration sheds light on the molecular mechanisms involved in Tnmd-mediated tendon repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Mauricio R. Galiano, Victor E. Goitea, Marta E. Hallak
JOURNAL OF NEUROCHEMISTRY
(2016)
Article
Immunology
Aleksandra Urban, Anna Borowska, Anna Felberg, Lambertus van den Heuvel, Grzegorz Stasilojc, Elena Volokhina, Marcin Okroj
Editorial Material
Hematology
Grzegorz Stasilojc, Anna Felberg, Marcin Okroj
BRITISH JOURNAL OF HAEMATOLOGY
(2018)
Article
Neurosciences
Andrea Comba, Laura V. Bonnet, Victor E. Goitea, Marta E. Hallak, Mauricio R. Galiano
MOLECULAR NEUROBIOLOGY
(2019)
Article
Genetics & Heredity
Michel F. Guiraldelli, Anna Felberg, Luciana P. Almeida, Aniruddha Parikh, Rodrigo O. de Castro, Roberto J. Pezza
Article
Oncology
Anna Felberg, Aleksandra Urban, Anna Borowska, Grzegorz Stasilojc, Michal Taszner, Andrzej Hellmann, Anna Maria Blom, Marcin Okroj
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2019)
Article
Biochemical Research Methods
Grzegorz Stasilojc, Anna Felberg, Aleksandra Urban, Daria Kowalska, Shuo Ma, Anna M. Blom, Jeanette Lundin, Anders Osterborg, Marcin Okroj
JOURNAL OF IMMUNOLOGICAL METHODS
(2020)
Article
Allergy
Aleksandra Urban, Elena Volokhina, Anna Felberg, Grzegorz Stasilojc, Anna M. Blom, Ilse Jongerius, Lambertus van den Heuvel, Marcel Thiel, Stanislaw Oldziej, Emilia Arjona, Santiago Rodriguez de Cordoba, Marcin Okroj
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2020)
Article
Immunology
Anna Felberg, Michal Taszner, Aleksandra Urban, Alan Majeranowski, Kinga Jaskula, Aleksandra Jurkiewicz, Grzegorz Stasilojc, Anna M. Blom, Jan M. Zaucha, Marcin Okroj
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Klaire Yixin Fjaestad, Anne Mette Askehoj Romer, Victor Goitea, Astrid Zedlitz Johansen, Marie-Louise Thorseth, Marco Carretta, Lars Henning Engelholm, Lars Grontved, Niels Junker, Daniel Hargbol Madsen
Summary: The development of immune checkpoint inhibitors (ICI) has been an important breakthrough in cancer therapies, but only a limited number of patients benefit from them. Recently, the nonselective beta blocker propranolol has been shown to be effective in treating angiosarcoma and soft tissue sarcomas. It works by reducing tumor angiogenesis and promoting an anti-tumoral microenvironment with increased T cell infiltration. The combination of propranolol with immune checkpoint inhibitors can significantly enhance their efficacy.
Article
Cell Biology
Stine M. Praestholm, Catarina M. Correia, Victor E. Goitea, Majken S. Siersbaek, Mathilde Jorgensen, Jesper F. Havelund, Thomas A. Pedersen, Nils J. Faergeman, Lars Grontved
Summary: The transition from a fasted to a fed state leads to extensive transcriptional remodeling in hepatocytes, with a subset of hepatic feeding-induced genes requiring glucocorticoid receptor (GR) for full expression. This includes the important gene Gck, which is involved in hepatic glucose metabolism. GR cooperatively regulates hepatic Gck expression with insulin in a direct manner, affecting postprandial glucose uptake and storage in the liver.
Article
Genetics & Heredity
Rodrigo O. de Castro, Agustin Carbajal, Luciana Previato de Almeida, Victor Goitea, Courtney T. Griffin, Roberto J. Pezza
Summary: Testis development and germ cell production rely on spermatogonia stem cells. The Chd4 chromatin remodeling complex is important for gene expression and maintenance of spermatogonia stem cells during gamete development.
EPIGENETICS & CHROMATIN
(2022)
Article
Biochemistry & Molecular Biology
Anna Maria Drozd, Luca Mariani, Xiaogang Guo, Victor Goitea, Niels Alvaro Menezes, Elisabetta Ferretti
Summary: Progesterone plays a specific regulatory role in embryonic development by modulating extraembryonic and cardiac mesoderm differentiation. Its function in heart development emphasizes the need for close monitoring of progesterone administration during infertility treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)