Article
Food Science & Technology
Zhipeng Zheng, Li Wu, Wei Deng, Kexin Yi, Yibin Li
Summary: The experiment showed that plums at the ripe stage have the highest levels of polyphenols, flavonoids, and anthocyanins, as well as the strongest antioxidant capacity. The major phenolic compounds in plum polyphenols include flavan-3-ols, flavonols, and phenolic acids. Plum polyphenols also exhibit a strong inhibitory effect on xanthine oxidase (XOD).
Article
Chemistry, Physical
M. S. Raghu, K. Yogesh Kumar, M. K. Prashanth, V. S. Anusuya Devi, Fahd Alharethy, Byong-Hun Jeon
Summary: We developed a series of pyrazolo[1,5-a][1,3,5]triazine derivatives (5a-l) through condensation, intramolecular ring annulation, and acylation processes. These compounds exhibited moderate to substantial antioxidant activity and were effective in inhibiting LOX and XO enzyme activity. Particularly, compound 5f showed excellent antioxidant and enzyme inhibition abilities. Molecular docking studies further confirmed the correlation between docking scores and experimental antioxidant and enzyme inhibition activity.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Chemistry, Applied
Zhengzheng Zou, Claire Bouchereau-De Pury, Amitha K. Hewavitharana, Saad S. Al-Shehri, John A. Duley, David M. Cowley, Pieter Koorts, Paul N. Shaw, Nidhi Bansal
Summary: A high-throughput fluorescence assay method was developed for determination of oxidase activities in milk samples, showing high sensitivity, good repeatability and reproducibility. The method was successfully applied to survey different animal milk samples and can be adapted for measurements of other oxidase activities.
Review
Biochemistry & Molecular Biology
Mai Sekine, Ken Okamoto, Kimiyoshi Ichida
Summary: Xanthine oxidoreductase (XOR) is a key enzyme in purine metabolism, with complex structure and function well studied. XOR inhibitors are used to treat hyperuricemia and gout, with potential effects beyond uric acid reduction. Xanthinuria serves as a good model for studying XOR mutations and activity, shedding light on the biological role and reaction mechanism of XOR.
Article
Food Science & Technology
Yuchen Tian, Lianzhu Lin, Mouming Zhao
Summary: This study prepared lotus leaf extracts by multiple methods, identified alkaloids and flavonoids as contributors to their activities, established quality control standards for dual-function lotus leaf extracts, and determined the optimal approach for preparing food-grade lotus leaf extracts. Network pharmacology analysis revealed the potential of these extracts in improving hyperuricemia through various actions.
Article
Plant Sciences
Yuchen Tian, Lianzhu Lin, Mouming Zhao, An Peng, Kun Zhao
Summary: Moringa oleifera Lam. leaf (MOL) is rich in protein and phenolics, which have been traditionally used to treat various diseases. Recent studies have shown that MOL possesses antioxidant, anti-dyslipidemia, and hepato-renal protective activities, making it a potential agent to improve metabolic disorders associated with hyperuricemia. The antihyperuricemic effect of MOL hydrolysate (MOLH) with high contents of phenolics and peptides was explored in this study, and the results indicated that MOLH could effectively reduce serum uric acid levels and improve metabolic disorders.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Chemistry, Physical
Songul Eglemce-Bakir, Musa Sahin, Eda Erdemir, Mustafa Ozyurek, Bahri Ulkuseven
Summary: In this study, a series of long alkyl chain-substituted dioxomolybdenum(VI) complexes were successfully synthesized and characterized. Complex VII exhibited significant XO inhibitory activity and has the potential to be used in the treatment of XO-based disorders.
JOURNAL OF MOLECULAR STRUCTURE
(2024)
Article
Chemistry, Inorganic & Nuclear
Songul Eglence-Bakir, Musa Sahin, Mustafa Ozyurek, Bahri Ulkuseven
Summary: Eight dioxomolybdenum(VI) complexes of 4-benzyloxysalicylidene N- or S-alkyl substituted thiosemicarbazones were synthesized and characterized, with one crystal structure being determined. The total antioxidant capacities were measured using the CUPRAC method, showing higher TEAC values for ONN series compared to ONS series. Scavenging effects for hydroxyl radical and xanthine oxidase inhibition activities were also investigated.
Article
Environmental Sciences
Oumaima Boutoub, Soukaina El-Guendouz, Leticia M. Estevinho, Vanessa B. Paula, Smail Aazza, Lahsen El Ghadraoui, Brigida Rodrigues, Sara Raposo, Jorge Carlier, Maria C. Costa, Maria G. Miguel
Summary: Natural products, including Euphorbia resinifera and Euphorbia officinarum extracts and honeys, were found to have antioxidant properties and enzyme inhibitory activities. Higher metal content in E. officinarum honey may suggest environmental pollution. Extraction parameters influenced the phytochemical content and bioactivities of the extracts, with aqueous extracts generally showing higher in vitro activities compared to honey samples.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Samikshya Neupane, Simran Bajracharya, Sanju Thada, Anita Bakabal, Ram Bahadur Khadka, Hari Prasad Devkota, Jitendra Pandey
Summary: Choerospondias axillaris Roxb. is a deciduous tree native to Nepal and the Himalayan region. The study examined the phenol, flavonoid, and carbohydrate content, as well as the antioxidant and antibacterial properties of C. axillaris fruit extracts. The acetone extract showed the highest antioxidant activity and xanthine oxidase inhibition, as well as strong antibacterial efficacy against Staphylococcus aureus and Bacillus cereus. The findings suggest that C. axillaris extracts have potential applications in pharmaceutical and nutraceutical industries.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Analytical
Fuxin Liu, Tianyi Shang, Lingling Han, Nan He, Xiuhui Liu
Summary: Xanthine, a significant biomolecule, can be used as an indicator for specific pathological conditions. Researchers have developed a new sensing platform and used electrochemical method to study the kinetics of xanthine metabolic reaction for the first time. The results showed that the conversion of xanthine to uric acid followed Michaelis-Menten kinetics, and the inhibitory effect of febuxostat on xanthine oxidase activity was also studied.
Article
Biochemistry & Molecular Biology
Yoshiro Tanaka, Tomohisa Nagoshi, Akira Yoshii, Yuhei Oi, Hirotake Takahashi, Haruka Kimura, Keiichi Ito, Yusuke Kashiwagi, Toshikazu D. Tanaka, Michihiro Yoshimura
Summary: Accumulating evidence suggests that high serum uric acid is associated with left ventricular dysfunction, and xanthine oxidase activation plays a critical role in this process. In a study on doxorubicin-induced cardiotoxicity, it was found that cardiac tissue xanthine oxidase activation led to impaired left ventricular function, which was attenuated by xanthine oxidase inhibitors through reduction of oxidative stress and modulation of purine metabolism.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Medicine, Research & Experimental
Haiyang Yang, Xueyan Li, Gang Li, Huating Huang, Wenning Yang, Xiaoquan Jiang, Muli Sen, Jingjing Liu, Yang Liu, Yanli Pan, Guopeng Wang
Summary: This study emphasizes the importance of accurate quantitative determination of affinity and binding kinetics for tight binding inhibition. Recommendations on experimental design and mathematical calculation approaches are provided to evaluate affinity and BK parameters for inhibitors against xanthine oxidase. Significant differences in results and the influence of different data analytical methods on enzyme engagement in human are discussed.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Joao L. Serrano, Diana Lopes, Melani J. A. Reis, Renato E. F. Boto, Samuel Silvestre, Paulo Almeida
Summary: Sixteen bis-thiobarbiturates were synthesized with moderate to excellent reactional yields, showing good antioxidant performance and XO inhibitory activity. The most potent compound exhibited low cytotoxicity and about ten-fold better inhibition than the reference drug. Further analysis on the drug-likeness, pharmacokinetics, and toxicity of this promising barbiturate compound was also conducted.
Article
Biochemistry & Molecular Biology
Keum-Jin Yang, Won Jung Choi, Yoon-Kyung Chang, Cheol Whee Park, Suk Young Kim, Yu Ah Hong
Summary: Xanthine oxidase (XO) inhibition showed renoprotective effects in diabetic kidney disease (DKD) by inhibiting vascular endothelial growth factor (VEGF) and NADPH oxidase (NOX) and reducing oxidative stress. This study provides important insights into the therapeutic potential of XO inhibition in DKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Enkhtaivan Gansukh, Arti Nile, Doo Hwan Kim, Jae Wook Oh, Shivraj Hariram Nile
Summary: Quercetin and its derivatives were screened for their potential antiviral properties targeting the cap-binding site of the influenza viral RNA polymerase PB2 subunit. Among them, quercetin 3'-glucuronide (Q3G) showed the strongest binding affinity, while quercetin 3'-glucuronide (Q7G) exhibited the highest anti-influenza activity with low cytotoxic effects.
Review
Biotechnology & Applied Microbiology
Shivraj Hariram Nile, Guoyin Kai
Summary: The novel coronavirus SARS-CoV-2 causing COVID-19 has created significant health and economic burden globally, with no specific approved therapy or drug established for treatment. Clinical trials on natural plant-based medicines and Traditional Chinese medicine are being conducted to discover effective treatment and prevention strategies for COVID-19, showing promising results in using natural compounds like heparin and vitamin C, as well as Traditional Chinese medicine and herbal medicines.
INDIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Acoustics
Gopal Patel, Mahesh D. Patil, Sujit Tangadpalliwar, Shivraj Hariram Nile, Prabha Garg, Guoyin Kai, Uttam Chand Banerjee
Summary: This study focused on modeling and optimizing the production of mycophenolic acid (MPA) from Penicillium brevicompactum using ultrasonic stimulation. By optimizing the ultrasonic treatment conditions, the MPA production was significantly improved.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rohini Pungle, Shivraj Hariram Nile, Arun S. Kharat
Summary: Plant extracts were used to synthesize silver nanoparticles, which showed promising antimicrobial activity against multidrug-resistant Gram-negative clinical isolates.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Review
Food Science & Technology
Ramkumar Samynathan, Muthu Thiruvengadam, Shivraj Hariram Nile, Mohammad Ali Shariati, Maksim Rebezov, Raghvendra Kumar Mishra, Baskar Venkidasamy, Sureshkumar Periyasamy, Ill-Min Chung, Mirian Pateiro, Jose M. Lorenzo
Summary: Tea, made from the shoots of Camellia sinensis (L.) O. Kuntze, is the most commonly consumed nonalcoholic drink worldwide. The metabolites in tea have a wide range of therapeutic applications, particularly their antioxidant and disease-fighting properties in synergy with synthetic drugs. The bioactive flavonoids in tea also contribute to health benefits.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Agriculture, Multidisciplinary
Shivraj Hariram Nile, Baskar Venkidasamy, Ramkumar Samynathan, Arti Nile, Keding Shao, Tingting Chen, Meihong Sun, Muhammad Usman Khan, Nalok Dutta, Muthu Thiruvengadam, Mohammad Ali Shariati, Maksim Rebezov, Guoyin Kai
Summary: Soybean processing waste has the potential to be a sustainable source of phytochemicals and functional foods. Various analytical methods have been used to characterize phytochemicals, nutrients, and minerals from soybean processing waste. Utilization of this waste can increase production of bioactive compounds, nutritional supplements, and cosmetic ingredients, while also reducing environmental pollution through proper use.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Shivraj Hariram Nile, Yi Liang, Zengyuan Wang, Jiayi Zheng, Chengtao Sun, Arti Nile, Gopal Patel, Guoyin Kai
Summary: The ethanol extract of ashwagandha roots contains bioactive compounds that demonstrate antioxidant, anti-inflammatory, and cytotoxic effects on lung cancer cells. These findings suggest that ashwagandha could be a potential candidate for the treatment of inflammation, oxidative stress, and lung cancer.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Eun Young Ko, Shivraj Hariram Nile
Summary: Different cooking methods have an impact on the composition and biological properties of organosulfur compounds in onions. Stir-frying is an effective method to retain the highest amount of health-beneficial organosulfur compounds in onions.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Review
Biotechnology & Applied Microbiology
Shivraj Hariram Nile, Muthu Thiruvengadam, Yao Wang, Ramkumar Samynathan, Mohammad Ali Shariati, Maksim Rebezov, Arti Nile, Meihong Sun, Baskar Venkidasamy, Jianbo Xiao, Guoyin Kai
Summary: Nano-priming is an innovative seed priming technology that improves seed germination, growth, and yield by providing resistance to various stresses in plants. It induces multiple mechanisms such as breaking seed dormancy and promoting seed germination, leading to the production of primary and secondary metabolites in plants.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Cell Biology
Rohini Pungle, Shivraj Hariram Nile, Nilesh Makwana, Ragini Singh, Rana P. Singh, Arun S. Kharat
Summary: Green synthesis of silver nanoparticles using Tridax procumbens leaf extract showed antimicrobial and anticancer activities.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Editorial Material
Plant Sciences
Muthu Thiruvengadam, Shivraj Nile, Baskar Venkidasamy
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Pharmacology & Pharmacy
Yuqin Zhang, Liping Hua, Chunfeng Lin, Mingzhou Yuan, Wei Xu, Anand D. Raj, Baskar Venkidasamy, Carlos L. Cespedes-Acuna, Shivraj Hariram Nile, Guohong Yan, Haiyin Zheng
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Food Science & Technology
Asha S. Salunke, Shivraj Hariram Nile, Arun S. Kharat
Summary: This study identified potential fibrinolytic enzymes from six Bacillus spp. isolated from fruit and vegetable waste. The purified enzymes showed excellent fibrinolytic activity and could significantly degrade blood clot.
Article
Biochemistry & Molecular Biology
Shivraj Hariram Nile, Dipalee Thombre, Amruta Shelar, Krithika Gosavi, Jaiprakash Sangshetti, Weiping Zhang, Elwira Sieniawska, Rajendra Patil, Guoyin Kai
Summary: In this study, biogenic selenium nanoparticles were prepared and functionalized with nystatin to inhibit the growth, morphogenesis, and biofilm of Candida albicans. The nanoparticles were spherical in shape with a size range of 220-240 nm. The functionalization of nystatin on the nanoparticles was confirmed, and the nanoparticles showed antifungal activity on C. albicans. The nanoparticles act through the RAS/cAMP/PKA signaling pathway, and they are not cytotoxic.
Review
Nanoscience & Nanotechnology
Amruta Shelar, Shivraj Hariram Nile, Ajay Vikram Singh, Dirk Rothenstein, Joachim Bill, Jianbo Xiao, Manohar Chaskar, Guoyin Kai, Rajendra Patil
Summary: Agro seeds are susceptible to environmental stress, harming seed vigor, crop growth, and productivity. Chemical seed treatments can enhance germination but they also have negative environmental impacts. Sustainable nano-based agrochemicals can improve seed viability and control release of active ingredients, but their application raises concerns about nanomaterial safety, exposure, and toxicity to the environment and human health.
NANO-MICRO LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Manigandan Nagarajan, Gobichettipalayam Balasubramaniam Maadurshni, Jeganathan Manivannan
Summary: This study found that exposure to BPA exacerbates hypertension-induced pathological changes, including renal fibrosis, oxidative stress, elevated angiotensin-converting enzyme activity, dysfunction of antioxidant and tricarboxylic acid cycle enzymes, tissue lipid abnormalities, and expression of inflammatory factors. In addition, high levels of BPA have dose-dependent effects on kidney cells, including oxidative stress, mitochondrial dysfunction, and lipid peroxidation. Therefore, a precise investigation of BPA exposure in hypertensive populations is highly recommended during risk assessment.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Haiping Bao, Qiang Zhang, Yu Li, Chen Nie
Summary: In this study, the role of circDLGAP4 in Parkinson's disease (PD) was investigated. The results showed that circDLGAP4 upregulated HMGA2 by recruiting EIF4A3, thus alleviating neuronal injury in PD.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Lina Yang, Tianwei He, Yanming Yu
Summary: This study found that uric acid induces kidney injury by inducing IL-17 expression, and treatment with anti-IL-17 antibody can alleviate uric acid-induced kidney injury.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ediandra T. Castro, Amalia G. Alves, Daniela de Bittencourt Maia, Larissa S. Magalhaes, Mariana P. Paim, Filipe Penteado, Caroline S. Gomes, Eder J. Lenardao, Cesar A. Bruning, Cristiani F. Bortolatto
Summary: This study investigated the antioxidant activity and toxicity of selenium-containing compounds in liver tissue. The results showed that these compounds have potential antioxidant effects, which can be attributed to their ability to scavenge free radicals and mimic enzyme activity. Additionally, one of the compounds exhibited low toxicity.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2024)
