Article
Immunology
Gabriele Sass, Julianne J. Marsh, Pallabi Shrestha, Raquel Sabino, David A. Stevens
Summary: Cystic fibrosis patients frequently suffer from co-infections of Pseudomonas aeruginosa and Aspergillus fumigatus, which can cause airway inflammation and worsen lung function. Recent studies have shown that P. aeruginosa interacts synergistically with the antifungal drug voriconazole, inhibiting A. fumigatus biofilm metabolism. This interaction is mediated by several molecules, including pyoverdine. Clinical isolates of P. aeruginosa from CF patients show varying degrees of interaction with voriconazole, suggesting that lower concentrations of the drug might be sufficient to control fungal growth during co-infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Dirk Westhoelter, Jonas Raspe, Hendrik Uebner, Johannes Pipping, Mona Schmitz, Svenja Strassburg, Sivagurunathan Sutharsan, Matthias Welsner, Christian Taube, Sebastian Reuter
Summary: The study found that treatment with elexacaftor/tezacaftor/ivacaftor can increase the percentage of Tregs, especially in CF patients clearing Pseudomonas aeruginosa infection. This suggests that targeting Treg homeostasis is a therapeutic option for CF patients with persistent Treg impairment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Rachith Kalgudi, Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz
Summary: The study revealed that the combined use of farnesol and tyrosol had a better effect on Pseudomonas aeruginosa biofilm growth, especially against the RBHi strain, while the addition of quorum quenchers (QQs) reduced IL-8 secretion by A549 cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Microbiology
Christina S. Thornton, Michael G. Surette
Summary: Cystic fibrosis is the most common and lethal genetic disease among the Caucasian population, leading to chronic airway inflammation and declining pulmonary function. Studies have shown a diverse community of anaerobic bacteria in CF patients' airways, potentially impacting disease progression through synergistic interaction with principal pathogens. Despite the understanding of this complex bacterial milieu, the specific roles of anaerobes in disease progression remain unclear.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Xuerui Bao, Mona Bove, Tom Coenye
Summary: The high tolerance of Pseudomonas aeruginosa biofilms in respiratory tract infections in cystic fibrosis contributes to the failure of antibiotic therapy. This study identified carbon sources that could enhance the inhibiting and/or eradicating activity of tobramycin, ciprofloxacin, and ceftazidime against P. aeruginosa PAO1 biofilms. The mechanisms underlying the enhanced biofilm eradicating activity were strain-dependent.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Immunology
Veronica Roxana Flores-Vega, Silvia Yalid Vargas-Roldan, Jose Luis Lezana-Fernandez, Ricardo Lascurain, Jose Ignacio Santos-Preciado, Roberto Rosales-Reyes
Summary: Cystic fibrosis is a genetic disease caused by a mutation in the cftr gene, affecting chloride ion and water transport and leading to bacterial infections in the lungs. Autophagy plays a crucial role in pathogen clearance, but P. aeruginosa and B. cenocepacia have strategies to evade this pathway, resulting in chronic inflammatory immune responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Tao Li, Yingjie Song, Liming Luo, Ninglin Zhao, Lihui He, Mei Kang, Changcheng Li, Yibo Zhu, Yalin Shen, Chang Zhao, Jing Yang, Qin Huang, Xingyu Mou, Zhiyong Zong, Jinliang Yang, Hong Tang, Yongxing He, Rui Bao
Summary: AlgW, a membrane-bound periplasmic serine protease belonging to the HtrA protein family, can be biochemically activated by both peptide and lipid signals. It exhibits distinct substrate specificity and regulation mechanism compared to its homologue DegS in Escherichia coli. AlgW can adopt a more relaxed conformation and obtain higher activity by specifically binding to the C-terminal decapeptide of MucE. The unique structural features of AlgW, including the regulatory mechanism of the LA loop, contribute to its distinct enzymatic property.
Article
Biochemistry & Molecular Biology
Sonal Mahajan, Sonali Sunsunwal, Vikas Gautam, Meenu Singh, T. N. C. Ramya
Summary: The study assessed the efficacy of a panel of alginate lyases in combating mucoid Pseudomonas aeruginosa biofilms in cystic fibrosis patients, revealing that some alginate lyases can inhibit biofilm formation by mucoid P. aeruginosa, showing therapeutic potential and warranting further exploration in future studies.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Immunology
Jonathan D. Cogen, Anna Faino, Frankline Onchiri, Lucas R. Hoffman, Matthew P. Kronman, David P. Nichols, Margaret Rosenfeld, Ronald L. Gibson
Summary: A retrospective cohort study found that there were no significant differences in respiratory and clinical outcomes between young patients with cystic fibrosis treated with one versus two intravenous antipseudomonal antibiotics for pulmonary exacerbations.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Giovanni Di Bonaventura, Veronica Lupetti, Simone De Fabritiis, Alessandra Piccirilli, Annamaria Porreca, Marta Di Nicola, Arianna Pompilio
Summary: This study evaluated the antibacterial and antivirulence activities of eight FDA-approved non-antibiotic drugs and their effects on biofilm formation. Ciclopirox and actinomycin D showed the best antibacterial activity and were most effective against preformed biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Medicine, Research & Experimental
Roberto Rosales-Reyes, Veronica Roxana Flores-Vega, Jose Luis Lezana-Fernandez, Jose Ignacio Santos-Preciado
Summary: Pseudomonas aeruginosa is a significant pathogen causing lung infections in patients with cystic fibrosis. Its increasing antibiotic resistance makes treatment challenging. Microbiology and whole genome sequencing are important tools for determining antimicrobial susceptibility and resistance as well as analyzing genomic variations. Molecular epidemiologic surveillance aids in developing better treatment strategies and controlling the spread of Pseudomonas aeruginosa variants.
ARCHIVES OF MEDICAL RESEARCH
(2022)
Article
Immunology
Jonathan D. Cogen, Frankline M. Onchiri, Nicole Mayer Hamblett, Ronald L. Gibson, Wayne J. Morgan, Margaret Rosenfeld
Summary: The study showed that prolonged use of oral antibiotics in children with cystic fibrosis increased the risk of acquiring Achromobacter xylosoxidans, while treatment with intravenous antibiotics was associated with an increased risk of acquiring multidrug-resistant Pseudomonas aeruginosa and MRSA.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Giovanni Di Bonaventura, Veronica Lupetti, Fabio Verginelli, Sara Giancristofaro, Rosemary Barbieri, Giovanni Gherardi, Arianna Pompilio
Summary: This study evaluated the antibacterial, antibiofilm, and antivirulence activities of apramycin compared to tobramycin against P. aeruginosa in cystic fibrosis patients. Apramycin showed better efficacy than tobramycin, without cytotoxicity to bronchial epithelial CF cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Aleksandra John, Joanna Gozdzik-Spychalska, Magdalena Durda-Masny, Wojciech Czainski, Natalia Pawlowska, Jolanta Wlizlo, Halina Batura-Gabryel, Anita Szwed
Summary: The study described the relationship between the prevalence of Pseudomonas aeruginosa (PA) and lung function, nutritional status, and gene mutation type in adult patients with cystic fibrosis (CF). The presence of PA was associated with lower FEV1% and BMI values. Severe gene mutation, undernutrition, and deterioration of lung function were linked to a higher probability of PA acquisition and resistance to antibiotic treatment.
Review
Medicine, Research & Experimental
Roberto Rosales-Reyes, Silvia Yalid Vargas-Roldan, Jose Luis Lezana-Fernandez, Jose Ignacio Santos-Preciado
Summary: Cystic fibrosis is a genetic disease that affects the respiratory and digestive systems, causing chronic lung infections and breathing difficulties. Mutations in the CFTR gene lead to imbalanced intracellular water content, resulting in sticky mucus and bacterial infections that ultimately affect lung function.
ARCHIVES OF MEDICAL RESEARCH
(2021)