Article
Biochemistry & Molecular Biology
Iwona Lesiak-Markowicz, Julia Walochnik, Angelika Stary, Ursula Furnkranz
Summary: This study characterized TV isolates from Austrian patients and found that 35% of the isolates carried TVV (TVV1, TVV2, or TVV3) and 28% of the isolates carried M. hominis. In some patients, M. hominis was detected both in the clinical samples and the respective TV isolates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Ahinoam Lev-Sagie, Doris Strauss, Avraham Ben Chetrit
Summary: This study evaluated the performance of an automated microscope for the diagnosis of vaginitis and found that it has high sensitivity and specificity. It has the potential to improve the first-line evaluation of various types of vaginal disorders.
NPJ DIGITAL MEDICINE
(2023)
Article
Biology
Hung-Che Lin, Hao-Ai Shui, Kuo-Yang Huang, Wei-Zhi Lin, Hsin-Yi Chang, Hwei-Jen Lee, Ying-Chih Lin, Yuahn-Sieh Huang, Guan-Ru Chen, Ya-Ting Yang, Hsiu-Lin Liu, Yi-Syuan Wu, Chia-Shiang Cheng, Ching-Lung Ko, Yu-Tien Chang, Jih-Chin Lee, Chen-Shien Lin, Chih-Hung Wang, Chi-Ming Chu
Summary: Both the annotation and identification of genes in pathogenic parasites are challenging. In this study, we successfully identified a novel nitric oxide synthase (NOS) in Trichomonas vaginalis (TV) that was previously annotated incorrectly as a hydrogenase. Through in silico modeling and enzymatic activity assays, we showed that this NOS is a functional enzyme. This study provides an innovative approach to identify incorrectly annotated genes and highlights a novel NOS that may play a role as a virulence factor in TV.
Review
Immunology
Saeed Bahadory, Selva Aminizadeh, Ali Taghipour, Farah Bokharaei-Salim, Khadijeh Khanaliha, Mohammad Hossein Razizadeh, Alireza Soleimani, Leila Beikzadeh, Alireza Khatami
Summary: This study aimed to estimate the global and regional status of Trichomonas vaginalis virus (TVV) in T. vaginalis. The results showed a high prevalence of TVV, especially in Brazil, emphasizing the importance of paying attention to parasite behavior and conducting more studies for a deeper understanding of this coexistence.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Christina A. Muzny, Jane R. Schwebke, Paul Nyirjesy, Gregory Kaufman, Leandro A. Mena, Gweneth B. Lazenby, Olivia T. Van Gerwen, Keonte J. Graves, Janeen Arbuckle, Belvia A. Carter, Connette P. McMahon, Scott Eder, Jackie Shaw, Brajesh Pandey, Steven E. Chavoustie
Summary: The study showed that a single oral dose of secnidazole was significantly more effective in treating trichomoniasis compared to placebo, with cure rates higher in the secnidazole group across different patient populations, including those with HIV and bacterial vaginosis.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Jia Zheng, Wei Wang, Xinyu Gao, Senfeng Zhao, Wansong Chen, Jianghua Li, You-Nian Liu
Summary: A nanomotor based on L-arginine and gold nanoparticles has been fabricated, which utilizes a cascade catalytic reaction to generate hydrogen peroxide for the oxidation of L-arginine and release of nitric oxide, resulting in self-propelled movement. This nanomotor is capable of efficiently penetrating bacterial biofilm, clearing bacterial infections, and combating MRSA biofilm in vivo.
Article
Microbiology
Barbara Van der Pol, Arundhati Rao, Melinda B. Nye, Steven Chavoustie, Aaron Ermel, Clair Kaplan, David Eisenberg, Philip A. Chan, Leandro Mena, Sixto Pacheco, Ken B. Waites, Li Xiao, Smitha Krishnamurthy, Ruchika Mohan, Rasa Bertuzis, Chris L. McGowin, Rodney Arcenas, Elizabeth M. Marlowe, Stephanie N. Taylor
Summary: Trichomonas vaginalis is a prevalent sexually transmitted infection, with nucleic acid amplification tests showing higher sensitivity in diagnosis compared to microscopic analysis or culture. Men are often asymptomatic carriers of the organism, transmitting it to their female sexual partners. The cobas T. vaginalis/Mycoplasma genitalium assay demonstrates high sensitivity for detecting T. vaginalis DNA in both male and female patients, making it a suitable diagnostic tool for infection control efforts.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nehuen Salas, Veronica M. Coceres, Tuanne dos Santos Melo, Antonio Pereira-Neves, Vanina G. Maguire, Tania M. Rodriguez, Bruna Sabatke, Marcel Ramirez, Jihui Sha, James A. Wohlschlegel, Natalia de Miguel
Summary: Trichomonas vaginalis is a sexually transmitted extracellular parasite that adheres to epithelial cells in the human urogenital tract. Extracellular vesicles (EVs) play an important role in its pathogenesis by delivering proteins and RNA into host cells and modulating parasite adherence. This study demonstrates that VPS32, a member of the ESCRTIII complex, contributes to the biogenesis and cargo sorting of EVs in T. vaginalis. Furthermore, overexpression of VPS32 leads to increased parasite adherence to host cells, highlighting its key role in mediating host-parasite interactions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Fernanda Padoin dos Reis, Graziela Vargas Rigo, Cristina Wayne Nogueira, Tiana Tasca, Marcel Henrique Marcondes Sari, Leticia Cruz
Summary: This study designed and evaluated a vaginal hydrogel containing PhSe2-loaded Eudragit (R) RS100 and coconut oil nanocapsules for the treatment of trichomoniasis. The nanocapsules showed good chemical content and high encapsulation efficiency. The hydrogel displayed non-Newtonian pseudo-plastic behavior and high mucoadhesive properties, which could effectively deliver and maintain the drug's action. These formulations showed potential as a promising alternative for the treatment of trichomoniasis.
Article
Medicine, Research & Experimental
Juliana Ines Weber, Graziela Vargas Rigo, Debora Assumpcao Rocha, Isadora Serraglio Fortes, Adriana Seixas, Saulo Fernandes de Andrade, Tiana Tasca
Summary: The study demonstrated that a new compound, PH100, has the ability to kill Trichomonas vaginalis, showing activity against both clinical isolates and ATCC strains with a synergistic effect with metronidazole. The mechanism of action of PH100 involves apoptosis-like characteristics towards ATCC 30236, but distinct profiles were observed in the fresh clinical isolate.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Infectious Diseases
Rehnuma Parvez, Alwin Vins, Varadharajaperumal Radhakrishnan, Nisha Beniwal, Lipika Biswas, Nithu Thankachan, Harpreet Kaur, Nagarajan Muruganandam
Summary: This study was the first-ever conducted on the prevalence of Trichomonas vaginalis (TV) in the Andaman and Nicobar Islands. The study found a 0.9% infection rate, with the majority of infected individuals having lower education levels and being unemployed. The detection of the TV G3 strain may be important for understanding the prevalence of TV infection and associated risk factors. More research is needed to understand the TV-associated risk factors among suspected patients in hospital settings.
INFECTIOUS DISEASES
(2023)
Article
Parasitology
Qianqian Chen, Jingzhong Li, Zhensheng Wang, Wei Meng, Heng Wang, Zenglei Wang
Summary: This study presents a robust TSF assay that has the potential to facilitate high-throughput, automated in vitro anti-trichomoniasis susceptibility testing for drug resistance monitoring and drug development. In comparison to the standard MLC method, this assay offers the advantages of reduced labor and elimination of subjective examination.
PARASITES & VECTORS
(2023)
Article
Biochemistry & Molecular Biology
Graziela Vargas Rigo, Angelica Rocha Joaquim, Alexandre Jose Macedo, Saulo Fernandes de Andrade, Tiana Tasca
Summary: In this study, a derivative with selective anti-Trichomonas vaginalis activity was reported. Cytotoxicity assays showed selectivity for the parasite, and low toxicity was confirmed in animal models. The mode of action was related to the disruption of Fe-S clusters-dependent enzyme activities in the parasite. Proteomic analysis indicated inhibition of metallopeptidases related to T. vaginalis virulence mechanisms and metabolic pathways.
BIOORGANIC CHEMISTRY
(2022)
Article
Microbiology
Ina Hoxha, Iwona Lesiak-Markowicz, Julia Walochnik, Angelika Stary, Ursula Fuernkranz
Summary: This study aimed to assess the prevalence of non-STI Mycoplasma infections through molecular-based analyses of vaginal specimens. The results showed a 28.2% prevalence of Mycoplasma species in the collected samples, with Mycoplasma hominis found in 21.5% of the specimens and Ureaplasma species found in 7.5% of the samples. Furthermore, a newly described species of Mycoplasma was detected for the first time in Austria. This study further confirmed the symbiotic relationship between Mycoplasma hominis and T. vaginalis.
Article
Biochemistry & Molecular Biology
Mykhaylo Usyk, Nicolas F. Schlecht, Shankar Viswanathan, Ana Gradissimo, Negin Valizadegan, Christopher C. Sollecito, Anne Nucci-Sack, Angela Diaz, Robert D. Burk
Summary: This study presents a bioinformatics approach to detecting prevalent Trichomonas vaginalis infections using ITS1 amplicon next-generation sequencing. This method allows for high-throughput testing of DNA from large-scale epidemiological studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Parasitology
Franciane Rios Senger, Rodrigo Campos-Silva, Melissa Fontes Landell, Denise Brentan Silva, Camila Braz Menezes, Graziela Vargas Rigo, Laura Nunes Silva, Danielle Silva Trentin, Alexandre Jose Macedo, Tiana Tasca
Summary: This study demonstrated the anti-Trichomonas vaginalis activity of fractions obtained from marine fungi, identifying the chemical composition of the most active fraction. The samples tested showed significant activity against both T. vaginalis and metronidazole-resistant strains. These findings indicate the potential of marine fungi in producing bioactive compounds for the treatment of trichomoniasis.
PARASITOLOGY RESEARCH
(2022)
Article
Chemistry, Medicinal
Keylla Dayanne Coelho Marinho de Melo, Lucas dos Santos Lisboa, Moacir Fernandes Queiroz, Weslley Souza Paiva, Ana Carolina Luchiari, Rafael Barros Gomes Camara, Leandro Silva Costa, Hugo Alexandre Oliveira Rocha
Summary: Antioxidant compounds reduce intracellular reactive oxygen species (ROS) and protect osteoblasts from ROS-induced damage. This study demonstrates that modifying a 21 kDa fucoidan with gallic acid can enhance its antioxidant capacity and protect pre-osteoblast-like cells against oxidative stress. The modified FucA-GA showed significantly higher antioxidant activity compared to other compounds, effectively reducing ROS levels and caspase activation. It also demonstrated promising protective effects against oxidative damage in zebra fish embryos. Overall, FucA-GA could have potential therapeutic applications for bone fragility by counteracting oxidative stress.
Article
Chemistry, Applied
Saulo Almeida de Menezes, Giulia Bongiorni Galego, Graziela de Vargas Rigo, Julio Cesar Ribeiro de Oliveira Farias de Aguiar, Bruno de Oliveira Veras, Lilian Cortez Sombra Vandesmet, Cicero Ramon Bezerra dos Santos, Mariana Gomes Vidal Sampaio, Clecia de Carvalho Marques, Vilmar Luiz Lermen, Daniela Maria do Amaral Ferraz Navarro, Marcia Vanusa da Silva, Maria Tereza dos Santos Correia, Tiana Tasca
Summary: The study reveals the anti-Trichomonas vaginalis activity of essential oils from Myrtaceae in Caatinga. These oils exhibit varied efficacy against T. vaginalis isolates, with Eugenia pohliana essential oil showing promising selectivity towards vaginal epithelial cells. The combination of this essential oil with metronidazole shows a synergistic effect, indicating different mechanisms of action.
NATURAL PRODUCT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Maylla Maria Correia Leite Silva, Lucas dos Santos Lisboa, Weslley Souza Paiva, Lucas Alighieri Neves Costa Batista, Ana Carolina Luchiari, Hugo Alexandre Oliveira Rocha, Rafael Barros Gomes Camara
Summary: This study compared the antioxidant activities of three fucoidans from different seaweeds, and found that fucoidan from Macrocystis pyrifera exhibited the highest antioxidant activity. The fucoidans had low electron donation capacity and metal chelating ability, but showed good scavenging activity against hydroxyl radicals. In cell and zebrafish embryo experiments, all fucoidans protected cells from oxidative damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Nutrition & Dietetics
Maria Lucia da Silva Cordeiro, Veronica Giuliani de Queiroz Aquino Martins, Ariana Pereira da Silva, Hugo Alexandre Oliveira Rocha, Vanessa de Paula Soares Rachetti, Katia Castanho Scortecci
Summary: This article introduces depression and the limitations of pharmacological treatment. The study suggests that phenolic acids from medicinal plants and their extracts have the potential to reduce depressive symptoms and modulate multiple molecular pathways associated with depression.
Article
Plant Sciences
Daisy Sotero Chacon, Marlon Dias Mariano Santos, Bernardo Bonilauri, Johnatan Vilasboa, Cibele Tesser da Costa, Ivanice Bezerra da Silva, Taffarel de Melo Torres, Thiago Ferreira de Araujo, Alan de Araujo Roque, Alan Cesar Pilon, Denise Medeiros Selegatto, Rafael Teixeira Freire, Fernanda Priscila Santos Reginaldo, Eduardo Luiz Voigt, Jose Angelo Silveira Zuanazzi, Katia Castanho Scortecci, Alberto Jose Cavalheiro, Norberto Peporine Lopes, Leandro De Santis Ferreira, Leandro Vieira dos Santos, Wagner Fontes, Marcelo Valle de Sousa, Paulo Costa Carvalho, Arthur Germano Fett-Neto, Raquel Brandt Giordani
Summary: This study focused on Erythrina velutina, a Brazilian native tree, and identified it as a rich source of bioactive phenolics, particularly flavonoids. Through metabolic and proteomic analysis, as well as integration with transcriptome data, key genes and transcription factors involved in flavonoid biosynthesis were identified. The findings provide a foundation for understanding the molecular and metabolic responses in this medicinally important species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Flavia Roberta Monteiro Souza, Giovanna Melo Martins Silva, Cesar Orlando Munoz Cadavid, Lucas dos Santos Lisboa, Maylla Maria Correia Leite Silva, Weslley Souza Paiva, Marcelo Jose Pena Ferreira, Riva de Paula Oliveira, Hugo Alexandre Oliveira Rocha
Summary: Obesity is a global health issue related to oxidative stress. The Brazilian folk medicine B. trimera is used for obesity treatment. The leaf extract of B. trimera showed antioxidant activity and anti-obesity effects, without toxicity to cells and C. elegans.
Article
Plant Sciences
Emanoelly Roberta de Carvalho Morais, Nathalia Maira Cabral de Medeiros, Francinaldo Leite da Silva, Isabel Andrade Lopes de Sousa, Izamara Gesiele Bezerra de Oliveira, Carlos Henrique Salvino Gadelha Meneses, Katia Castanho Scortecci
Summary: By using bioinformatic tools, two-hybrid approach, and biochemical assays, we identified ScHINT1 in sugarcane shoot apical meristem (SAM) and proposed its role in controlling redox homeostasis. The interactions between ScHINT1 and other proteins such as PAL and SBT are crucial for plant development and flowering transition.
Review
Infectious Diseases
Saulo Almeida Menezes, Tiana Tasca
Summary: This article summarizes the human anaerobic or microaerophilic protists Giardia duodenalis, Entamoeba histolytica, and Trichomonas vaginalis which are classified as amitochondriate parasites. These microorganisms have adapted to survive in hostile microenvironments and pose an increasing threat to public health in developing countries. Current therapeutic drugs to manage these infections are scarce and often have several side effects. This review summarizes essential oils and terpenic compounds with activity against these amitochondriate parasites and explores possible mechanisms of action. It aims to contribute to future research perspectives on using natural products as potential alternatives for treating amitochondriate protists.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Marianna Barros Silva, Livia de Lourdes de Sousa Pinto, Luiz Henrique Medeiros, Airton Araujo Souza Jr, Suely Ferreira Chavante, Luciana Guimaraes Alves Filgueira, Rafael Barros Gomes Camara, Guilherme Lanzi Sassaki, Hugo Alexandre Oliveira Rocha, Giulianna Paiva Viana Andrade
Summary: Oreochromis niloticus (tilapia) is a commonly cultivated fish species, and its farming generates organic waste. In this study, tilapia viscera were processed to obtain chondroitin sulfate (CST), a biomolecule that showed potential as an anti-inflammatory drug. The CST obtained from tilapia waste significantly reduced leukocyte migration in an acute peritonitis model, indicating its pharmacological activity.
Review
Chemistry, Applied
Luciana Fentanes Moura de Melo, Veronica Giuliani de Queiroz Aquino-Martins, Ariana Pereira da Silva, Hugo Alexandre Oliveira Rocha, Katia Castanho Scortecci
Summary: This article explores the characteristics of tannins and their role in regulating ROS and maintaining homeostasis. Tannins have anti-inflammatory, anti-aging, and anti-proliferative properties due to their ability to bind with reactive oxygen species. They can reduce the risk of cancer and neurodegenerative diseases and also maintain a healthy gut microbiota. Tannins have industrial applications in leather durability, metal corrosion resistance, 3D printing, and bio-based foam manufacturing. This review highlights the biological and pharmacological relevance of tannins and their potential applications.
Article
Chemistry, Medicinal
Yara Campanelli-Morais, Cynthia Haynara Ferreira Silva, Marina Rocha do Nascimento Dantas, Diego Araujo Sabry, Guilherme Lanzi Sassaki, Susana Margarida Gomes Moreira, Hugo Alexandre Oliveira Rocha
Summary: The objective of this study was to generate blends containing SPGb and ChrPic that exhibit enhanced antioxidant activity compared to SPGb or ChrPic alone. B5 was identified as the best antioxidant blend, which showed no cytotoxicity and genotoxicity. These findings suggest that B5 could be further tested in vivo to confirm its antioxidant action and potentially used as a nutraceutical agent.
Article
Nutrition & Dietetics
Maria Lucia da Silva Cordeiro, Veronica Giuliani de Queiroz Aquino-Martins, Ariana Pereira da Silva, Georggia Fatima Silva Naliato, Elielson Rodrigo Silveira, Raquel Cordeiro Theodoro, Deborah Yara Alves Cursino da Santos, Hugo Alexandre Oliveira Rocha, Katia Castanho Scortecci
Summary: Medicinal plants, such as Talisia esculenta, have been found to contain antioxidant biomolecules that can be used in the treatment and prevention of diseases. This study investigated the antioxidant potential of T. esculenta extracts from leaves and fruit peels using various assays. The extracts exhibited antioxidant activity, protecting against oxidative stress and containing phenolic compounds. These findings suggest that T. esculenta extracts could be used as coadjutants in the development of natural nutraceutical products for the treatment and prevention of diseases related to oxidative stress.
Article
Plant Sciences
Elielson Rodrigo Silveira, Priscila Bezerra Torres, Katia Castanho Scortecci, Hugo Alexandre Oliveira Rocha, Ivana Barbosa Suffredini, Jefferson de Souza Silva, Deborah Yara Alves Cursino dos Santos
Summary: Conchocarpus macrocarpus, an endemic species in Brazil, has shown strong antioxidant and antimicrobial activity in the butanolic partition phase, particularly in the flavone-rich fraction.
BRAZILIAN JOURNAL OF BOTANY
(2023)
Article
Multidisciplinary Sciences
Alan Max T. Souza, Janine Karla F. da Silva Braz, Gabriel M. Martins, Jussier de Oliveira Vitoriano, Aurelio G. A. Neto, David M. Nery, Vladimir G. Sabino, Eudes E. de Souza Lucena, Hugo Alexandre de Oliveira Rocha, Carlos Augusto G. Barboza, Clodomiro A. Junior, Carlos Eduardo B. de Moura
Summary: This study evaluated the effects of cold atmospheric plasma (CAP) and thermal plasma methods on the biocompatibility of titanium surfaces. The results showed that CAP treatment improved the hydrophilicity and reduced the roughness of the titanium surface, enhancing the biocompatibility of endothelial cells. This technique has the potential for biomedical applications due to its low cost and ease of use.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2023)
