Article
Microbiology
Yi Wang, Mingxi Hua, Jingqiao Wang, Wen Xing, Jiatian Chen, Jingyuan Liu, Pengcheng Du
Summary: The emergence of high antimicrobial-resistant and hypervirulent Klebsiella pneumoniae (hvKp) clones in clinics, including non-CC258 subgroups, has become a concern. A retrospective study in a Chinese hospital revealed the presence of CC15 hvKp isolates, which were carbapenem-resistant and carried virulence plasmids. The isolates belonged to four lineages, and the spread of virulence plasmids contributed to the emergence of putative hvKp sublineages. These findings underscore the importance of surveillance in hospitals to control the transmission of non-CC258 subgroups.
Article
Microbiology
William C. Shropshire, An Q. Dinh, Michelle Earley, Lauren Komarow, Diana Panesso, Kirsten Rydell, Sara Gomez-Villegas, Hongyu Miao, Carol Hill, Liang Chen, Robin Patel, Bettina C. Fries, Lilian Abbo, Eric Cober, Sara Revolinski, Courtney L. Luterbach, Henry Chambers, Vance G. Fowler, Robert A. Bonomo, Samuel A. Shelburne, Barry N. Kreiswirth, David van Duin, Blake M. Hanson, Cesar A. Arias
Summary: Carbapenem-resistant Klebsiella pneumoniae (CRKp) infections in nosocomial settings pose a public health challenge, especially high-risk clones like clonal group 258 (CG258) associated with bla(KPC) carriage. The emergence of clonal group 307 (CG307) worldwide, while coexisting with CG258, is a recent concern. This study provides evidence of potential divergent evolution between CG258 and CG307, with limited sharing of adaptive genes. The unique setting of Houston, with co-circulation of both CRKp clones, allows for understanding of how differences in accessory genome content drive stable endemic populations of CRKp.
Article
Microbiology
Lucia Henrici De Angelis, Noemi Poerio, Vincenzo Di Pilato, Federica De Santis, Alberto Antonelli, Maria Cristina Thaller, Maurizio Fraziano, Gian Maria Rossolini, Marco Maria D'Andrea
Summary: Phage therapy for bacterial infections is being reconsidered, with a focus on understanding the molecular targets of phages. In a study on resistance to the lytic phage phi BO1E in Klebsiella pneumoniae, a mutant with a single mutation in the CPS biosynthesis gene was found to be less virulent, indicating a potential benefit of phage resistance for the host in the context of phage-bacteria-host interactions.
Article
Infectious Diseases
Yanyan Zhang, Xuemei Yang, Congcong Liu, Ling Huang, Lingbin Shu, Qiaoling Sun, Hongwei Zhou, Yonglu Huang, Chang Cai, Xiaoyan Wu, Sheng Chen, Rong Zhang
Summary: This study investigated the prevalence and characteristics of OXA-232 type carbapenemase in Zhejiang Province, China from 2018 to 2021. The results showed that OXA-232 is the most prevalent OXA-48-like derivative, and ST15 K. pneumoniae isolates are the major carriers. The transmission of ColKP3-type plasmid to E. coli highlighted the importance of understanding the transmission mechanism to prevent the spread of OXA-232 to other species.
INFECTIOUS DISEASES OF POVERTY
(2023)
Article
Microbiology
Yingying Hao, Xuguang Zhao, Cui Zhang, Yuanyuan Bai, Zhen Song, Xinglun Lu, Ran Chen, Yaoyao Zhu, Yueling Wang
Summary: Treatment options for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are limited, leading to renewed interest in combination therapy involving fosfomycin. However, reports of fosfomycin-resistant K. pneumoniae are on the rise. Monitoring of strains such as ST11-KL64 and ST11-KL47, which show higher resistance and virulence, is crucial to prevent future dissemination of resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Courtney L. Luterbach, Liang Chen, Lauren Komarow, Belinda Ostrowsky, Keith S. Kaye, Blake Hanson, Cesar A. Arias, Samit Desai, Jason C. Gallagher, Elizabeth Novick, Stephen Pagkalinawan, Ebbing Lautenbach, Glenn Wortmann, Robert C. Kalayjian, Brandon Eilertson, John J. Farrell, Todd McCarty, Carol Hill, Vance G. Fowler, Barry N. Kreiswirth, Robert A. Bonomo, David van Duin
Summary: This study evaluated the clustering of carbapenem-resistant Klebsiella pneumoniae (CRKp) among patients in US hospitals and identified widespread intrasystem and intersystem transmission. Different methods for assessing genetic similarity resulted in only minor differences in interpretation.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Einas A. A. Osman, Maho Yokoyama, Hisham N. N. Altayb, Daire Cantillon, Julia Wille, Harald Seifert, Paul G. Higgins, Leena Al-Hassan
Summary: This study sequenced the whole genomes of 86 Klebsiella pneumoniae isolates from hospitals in Khartoum, Sudan, and revealed their molecular epidemiology, virulence, and resistance profiles. The findings showed the presence of multiple resistance genes, including genes coding for ESBLs, carbapenemases, and aminoglycoside-modifying enzymes.
Article
Infectious Diseases
Xing Wang, Jie Qin, Guoxiu Xiang, Chen Wang, Qichen Wang, Juanxiu Qin, Haiying Wang, Zhen Shen
Summary: In this study, we collected IMP-4-producing Klebsiella pneumoniae isolates from paediatric patients at Shanghai Children's Medical Center. Whole-genome sequencing revealed a diverse population structure among IMP-4-producing isolates, with all blaIMP-4 genes located on plasmids. We observed clonal transmission of ST101 strains carrying IncHI5 blaIMP-4-harbouring plasmids and found that IncN plasmids were conjugative in transferring blaIMP-4. Our findings provide insights into the transmission dynamics and molecular features of IMP-4 in K. pneumoniae.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Rafael Nakamura-Silva, Mariana Oliveira-Silva, Joao Pedro Rueda Furlan, Eliana Guedes Stehling, Carlos Eduardo Saraiva Miranda, Andre Pitondo-Silva
Summary: This study characterized antimicrobial resistance, virulence factors, and clonal relationships among 13 K. pneumoniae strains belonging to CG258 in a tertiary hospital in northeastern Brazil. Most strains were MDR and XDR, with various resistance and virulence genes detected, as well as two hypermucoviscous strains and one capsular K-type 2. Sequence typing revealed ten different STs belonging to CG258, with two new STs described for the first time in this study.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Immunology
Qingqing Du, Fen Pan, Chun Wang, Fangyuan Yu, Yingying Shi, Wenxin Liu, Zhi Li, Ping He, Dingding Han, Hong Zhang
Summary: By conducting clinical, molecular, and phenotypic surveillance of hvKp strains in a pediatric population, it was found that K1-ST23 and K2-ST25 are high-risk clones of hvKp, and the genetic convergence of virulence and carbapenem-resistance is increasing among children, indicating the need for control measures to prevent dissemination in clinical settings.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Bo Fu, Dandan Yin, Chengtao Sun, Yingbo Shen, Dejun Liu, Rina Bai, Rong Zhang, Jianzhong Shen, Fupin Hu, Yang Wang
Summary: Increasing infections caused by bla(NDM)-carrying Klebsiella pneumoniae (NDMKP) pose an urgent threat to children with weakened immunity and limited antibiotic use. The isolates exhibit high resistance to carbapenems but are fully sensitive to other antibiotics. Molecular analysis reveals both clonal and horizontal transmission of bla(NDM) among NDM-KP strains in children's hospitals, with key plasmids and sequence type diversity contributing to their spread. This study provides important insights for pediatric clinicians in treating infections caused by NDM-KP.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Einas A. Osman, Nagwa E. El-Amin, Leena L. Al-Hassan, Maowia Mukhtar
Summary: The study investigated the molecular epidemiology of Klebsiella pneumoniae in major regional hospitals in Khartoum, Sudan, revealing 52 different sequence types and emphasizing the urgent need for systematic epidemiological studies on drug-resistant infections.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Xiong Liu, Kaiying Wang, Jiali Chen, Jingwen Lyu, Jinhui Li, Qichao Chen, Yanfeng Lin, Benshun Tian, Hongbin Song, Peng Li, Bing Gu
Summary: We report a nosocomial outbreak among pediatric patients caused by clonal dissemination of KPC-2-producing ST11 Klebsiella pneumoniae. Our findings highlight the value of whole-genome sequencing during outbreak investigations and illustrate that transmission chains can be identified during hospital stays.
MICROBIOLOGY SPECTRUM
(2022)
Review
Microbiology
Laura Joseph, Thomas Merciecca, Christiane Forestier, Damien Balestrino, Sylvie Miquel
Summary: Klebsiella pneumoniae is a Gram-negative pathogen responsible for community-acquired and nosocomial infections, with strains belonging to either multidrug-resistant or hypervirulent groups. This survey aims to provide an overview of murine models mimicking K. pneumoniae infections and bacterial functions, crucial for developing new therapeutics to limit these infections through modulation of immune responses and microbiota.
Article
Infectious Diseases
Jesus Machuca, Lorena Lopez-Cerero, Manuel Rodriguez-Maresca, Felipe Fernandez-Cuenca, Inmaculada Lopez-Hernandez, Mercedes Delgado-Valverde, Waldo Sanchez-Yebra, Alvaro Pascual
Summary: The aim of this study was to characterize a hospital outbreak of NDM-7-producing Klebsiella pneumoniae associated with the successful multidrug-resistant (MDR) high-risk clone ST11 between 2017 and 2019 in southern Spain. The outbreak involved a total of 46 NDM-7-producing isolates, all of which were MDR and carbapenem-resistant. Molecular typing revealed three different clones, and further analysis identified IncX3 plasmids carrying the NDM-7 carbapenemase.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)