Journal
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Volume 142, Issue 4, Pages 1229-+Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2017.10.023
Keywords
T(H)17 cells; dendritic cells; chronic inflammation; house dust extract; Toll-like receptor 4
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Funding
- National Institutes of Health, National Institute of Environmental Health Sciences [ZIA ES102025-09]
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Background: Mechanisms that elicit mucosal T(H)17 cell responses have been described, yet how these cells are sustained in chronically inflamed tissues remains unclear. Objective: We sought to understand whether maintenance of lung T(H)17 inflammation requires environmental agents in addition to antigen and to identify the lung antigen-presenting cell (APC) types that sustain the self-renewal of T(H)17 cells. Methods: Animals were exposed repeatedly to aspiration of ovalbumin alone or together with environmental adjuvants, including common house dust extract (HDE), to test their role in maintaining lung inflammation. Alternatively, antigen-specific effector/memory T(H)17 cells, generated in culture with CD4(+) T cells from Il17a fate-mapping mice, were adoptively transferred to assess their persistence in genetically modified animals lacking distinct lung APC subsets or cell-specific Toll-like receptor (TLR) 4 signaling. T(H)17 cells were also cocultured with lung APC subsets to determine which of these could revive their expansion and activation. Results: T(H)17 cells and the consequent neutrophilic inflammation were poorly sustained by inhaled antigen alone but were augmented by inhalation of antigen together with HDE. This was associated with weight loss and changes in lung physiology consistent with interstitial lung disease. The effect of HDE required TLR4 signaling predominantly in lung hematopoietic cells, including CD11c(+) cells. CD103(+) and CD11b(+) conventional dendritic cells interacted directly with T(H)17 cells in situ and revived the clonal expansion of T(H)17 cells both ex vivo and in vivo, whereas lung macrophages and B cells could not. Conclusion: T(H)17-dependent inflammation in the lungs can be sustained by persistent TLR4-mediated activation of lung conventional dendritic cells.
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