Article
Plant Sciences
Alba Alvarez-Morezuelas, Leire Barandalla, Enrique Ritter, Jose Ignacio Ruiz de Galarreta
Summary: In this study, 144 tetraploid potato genotypes were evaluated to investigate the variation of physiological and agronomic traits under control and water stress conditions. A total of 18 SNP markers significantly associated with these traits were identified, providing insights into the mechanisms of potato tolerance to water stress and potential markers for marker-assisted selection programs.
Article
Plant Sciences
A. Alvarez-Morezuelas, L. Barandalla, E. Ritter, M. Lacuesta, J. I. Ruiz de Galarreta
Summary: Due to climate change, water availability has become a major limiting factor in potato production. This study analyzed the differential response of physiological and yield components in six potato varieties under water deficit conditions and found that Monalisa showed the best response in tuber production under stress conditions. Indirect selection based on parameters associated with water stress can be a useful tool in potato breeding programs for the identification of more tolerant varieties.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Satish Kumar Sanwal, Parveen Kumar, Hari Kesh, Vijai Kishor Gupta, Arvind Kumar, Ashwani Kumar, Babu Lal Meena, Giuseppe Colla, Mariateresa Cardarelli, Pradeep Kumar
Summary: Salinity stress significantly reduces plant height, stem numbers, and tuber yield in potato varieties. Salt-tolerant genotypes exhibit better performance under salt stress due to a strong antioxidant defense system and osmotic adjustment, suggesting their potential for breeding new high-yielding, salt-tolerant lines. Specific physiological and biochemical traits were identified as predominant for tuber yield under salt stress, providing insights for further genetic and molecular studies on salt tolerance in potato.
Article
Agronomy
Ali Asad Bahar, Hafiz Nazar Faried, Kashif Razzaq, Sami Ullah, Gulzar Akhtar, Muhammad Amin, Mohsin Bashir, Nadeem Ahmed, Fahad Masoud Wattoo, Sunny Ahmar, Talha Javed, Manzer H. Siddiqui, Ferdinando Branca, Eldessoky S. Dessoky
Summary: Potassium fertilizer has a significant positive impact on potato crop growth and productivity, especially under partial root irrigation conditions. The study suggests that applying potassium at a rate of 100 kg per hectare is an effective approach to inducing drought tolerance in potato crops.
Review
Plant Sciences
Milan Kumar Lal, Rahul Kumar Tiwari, Awadhesh Kumar, Abhijit Dey, Ravinder Kumar, Dharmendra Kumar, Arvind Jaiswal, Sushil Sudhakar Changan, Pinky Raigond, Som Dutt, Satish Kumar Luthra, Sayanti Mandal, Madan Pal Singh, Vijay Paul, Brajesh Singh
Summary: In the face of climate change-induced heat and drought stress, the physiological, biochemical, and molecular responses of potatoes have undergone changes that ultimately affect tuber production and yield. Therefore, it is necessary to comprehensively screen germplasm and apply appropriate methods and strategies to cultivate potato cultivars that can thrive in drought-affected and heat-prone areas.
Article
Biochemistry & Molecular Biology
Jiangwei Yang, Ning Zhang, Jiangping Bai, Xiaoqin Duan, Luhe Zhang, Shengyan Liu, Xun Tang, Xin Jin, Shigui Li, Huaijun Si
Summary: This study demonstrates that stu-miR827 is involved in regulating the drought tolerance of potato by controlling the stomatal density. Suppression of stu-miR827 leads to higher stomatal density and weaker drought resistance in transgenic potato lines. StWRKY48 is identified as the target gene of stu-miR827 and its expression is induced by drought stress. The results suggest that suppression of stu-miR827 may negatively regulate the drought adaptation of potato by increasing stomatal density.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alba Alvarez-Morezuelas, Leire Barandalla, Enrique Ritter, Jose Ignacio Ruiz de Galarreta
Summary: In this study, the gene expression differences between control and water-stressed potato plants were analyzed using RNAseq. The variety Agria showed a better stress response compared to Zorba, with more differentially expressed genes (DEGs) detected. The most highly expressed gene under drought conditions was Abscisic acid and environmental stress-inducible protein TAS14-like. Several metabolic pathways and cellular components were found to be affected by water stress, providing valuable information for breeding more tolerant potato varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hongju Jian, Haonan Sun, Rongrong Liu, Wenzhe Zhang, Lina Shang, Jichun Wang, Vadim Khassanov, Dianqiu Lyu
Summary: This study used NGS and SMRT sequencing technology to study the transcription profiles in potato in response to drought stress. A total of 12,798 DEGs were identified at six time points, and GO and KEGG analysis revealed their involvement in response to drought stress through various pathways.
Article
Agronomy
Julia Hoelle, Folkard Asch, Awais Khan, Merideth Bonierbale
Summary: This study explores the suitability of combining remote sensing data with stress severity index (SSI) as a screening tool for selecting drought-tolerant potato genotypes. The results show that Delta T, PRI, and NDVI can serve as good indicators of genotype performance under drought conditions.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2023)
Article
Agronomy
Katharina Wellpott, Jannis Straube, Traud Winkelmann, Christin Buendig
Summary: Drought stress is a significant problem for potato production, especially with climate change and temperature peaks along with drought periods. This study aimed to analyze eight genes and determine their suitability for detecting early drought stress in potato. Six potato genotypes were examined for plant growth and physiological parameters after seven and 14 days of stress. Changes in gene expression were observed after seven days of stress for three genes: INH1, POD, and SBT1.7. The INH1 gene showed consistent upregulation in all genotypes and may be a suitable candidate for detecting drought stress in potato.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2023)
Article
Plant Sciences
Dominika Boguszewska-Mankowska, Krystyna Zarzynska, Beata Wasilewska-Nascimento
Summary: This experiment evaluated the effects of soil drought and high temperature stress on potato plants, and found that simultaneous application of both stresses had the greatest impact on plant morphology and yield.
Article
Agronomy
M. Kaium Chowdhury, M. A. Hasan, M. M. Bahadur, Md Rafiqul Islam, Md Abdul Hakim, Muhammad Aamir Iqbal, Talha Javed, Ali Raza, Rubab Shabbir, Sobhy Sorour, Norhan E. M. Elsanafawy, Sultana Anwar, Saud Alamri, Ayman El Sabagh, Mohammad Sohidul Islam
Summary: The increasing human population and changing climate have led to frequent drought spells, posing a serious threat to global food security. Identifying high yielding drought tolerant genotypes is an effective approach to combat these challenges. Through a field experiment, it was found that drought stress reduced the growth of wheat genotypes, with BAW 1169 showing the best adaptation and potential for future breeding programs.
Article
Plant Sciences
Liqin Liang, Liuyan Guo, Yifan Zhai, Zhiling Hou, Wenjing Wu, Xinyue Zhang, Yue Wu, Xiaona Liu, Shan Guo, Gang Gao, Weizhong Liu
Summary: In this study, 37 StSOS1 genes were identified and found to be unevenly distributed across 10 chromosomes in potato, with most of them located on the plasma membrane. These genes contain abundant cis-elements involved in various abiotic stress responses. Tissue specific expression showed that 21 of the 37 StSOS1 genes were widely expressed in various tissues or organs of the potato. Molecular interaction network analysis suggests that these genes may interact with other proteins involved in potassium ion transmembrane transport, response to salt stress, and cellular processes. Collinearity analysis showed orthologous StSOS1 genes in tomato, pepper, tobacco, and Arabidopsis. Moreover, the expression of StSOS1 genes were significantly modulated by various abiotic stresses, especially salt and abscisic acid stress. Subcellular localization in Nicotiana benthamiana suggested that StSOS1-13 was located on the plasma membrane. These results provide a comprehensive overview of the StSOS1 gene family and pave the way for further analysis of their function in SOS and hormone signaling pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Bas van den Herik, Sara Bergonzi, Christian W. B. Bachem, Kirsten ten Tusscher
Summary: The interaction between StSP6A and StSWEET11 in sucrose transport plays a crucial role in potato tuber yield, with a non-linear effect demonstrated.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Ghina Hajjar, Stephane Quellec, Sylvain Challois, Lydia Bousset-Vaslin, Gisele Joly, Christophe Langrume, Carole Deleu, Laurent Leport, Maja Musse
Summary: This study investigated the variations in biochemical traits of different tissues during potato tuber growth. MRI relaxometry was used to examine tissue-dependent metabolic patterns and differences between tissues. The T2 values did not significantly change during tuber development but were sensitive to water stress.
Article
Toxicology
Jimmy Alarcan, Heike Sprenger, Julia Waizenegger, Dajana Lichtenstein, Claudia Luckert, Philip Marx-Stoelting, Alfonso Lampen, Albert Braeuning
Summary: The study revealed differential regulation of genes in rat livers by different pesticides, with some genes related to xenobiotic metabolism and nuclear receptor activation affected. Mixtures of the pesticides showed similar effects on gene expression as individual compounds.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lisa Goedtke, Heike Sprenger, Ute Hofmann, Felix F. Schmidt, Helen S. Hammer, Ulrich M. Zanger, Oliver Poetz, Albrecht Seidel, Albert Braeuning, Stefanie Hessel-Pras
Summary: PAHs are environmental pollutants generated by incomplete combustion of organic matter, which can induce their own metabolism by activating AHR and CAR. Most PAHs activate AHR, while non-carcinogenic PAHs tend to activate CAR, leading to the expression of respective target genes. Additionally, an increased PAH ring number is associated with AHR activation and DNA double-strand breaks, while smaller PAHs activate CAR but do not cause DNA damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Stien Mertens, Lennart Verbraeken, Heike Sprenger, Kirin Demuynck, Katrien Maleux, Bernard Cannoot, Jolien De Block, Steven Maere, Hilde Nelissen, Gustavo Bonaventure, Steven J. Crafts-Brandner, Jonathan T. Vogel, Wesley Bruce, Dirk Inze, Nathalie Wuyts
Summary: Hyperspectral imaging is a useful tool for non-destructive phenotyping of plant physiological traits, especially for studying responses to drought, development, and day-night cycles. With better understanding of spectral measurements, it has been demonstrated to accurately predict physiological changes in plants under different conditions using advanced processing techniques such as partial least squares regression.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Genetics & Heredity
Christina Schumacher, Christoph Tim Krannich, Lisa Maletzki, Karin Koehl, Joachim Kopka, Heike Sprenger, Dirk Karl Hincha, Sylvia Seddig, Rolf Peters, Sadia Hamera, Ellen Zuther, Manuela Haas, Renate Horn
Summary: The study used a candidate gene approach to develop SSR markers for potato drought tolerance breeding. Validation showed that 49% of the SSR primer combinations resulted in polymorphic banding patterns with significant PIC values. Five SSR markers identified allelic differences associated with drought sensitivity in potato cultivars.
Article
Toxicology
Julia Buchmueller, Heike Sprenger, Johanna Ebmeyer, Josef Daniel Rasinger, Otto Creutzenberg, Dirk Schaudien, Jan G. Hengstler, Georgia Guenther, Albert Braeuning, Stefanie Hessel-Pras
Summary: This study investigated the gene expression changes in rat lungs and kidneys after exposure to six different PAs, highlighting inflammatory responses in lungs and cell-cycle regulation and DNA damage response in the liver. The results demonstrate tissue-specific molecular effects of PAs and provide valuable information on the early molecular effects in lung tissue after subacute exposure to PAs.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Christina Schumacher, Susanne Thumecke, Florian Schilling, Karin Kohl, Joachim Kopka, Heike Sprenger, Dirk Karl Hincha, Dirk Walther, Sylvia Seddig, Rolf Peters, Ellen Zuther, Manuela Haas, Renate Horn
Summary: The study identified genomic regions and candidate genes of major interest for drought tolerance in cultivated potatoes, as well as QTL and genes related to starch synthesis and metabolism. Overlaps of DRYM QTL with metabolite QTLs suggest possible trade-offs between starch and compatible solute biosynthesis. Mutations in genes under drought tolerance QTL on linkage group 3 were identified using BSAseq technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Dajana Lichtenstein, Almut Mentz, Heike Sprenger, Felix F. Schmidt, Stefan P. Albaum, Joern Kalinowski, Hannes Planatscher, Thomas O. Joos, Oliver Poetz, Albert Braeuning
Summary: The study focused on analyzing the mixture effects of different pesticides in exposure-relevant binary mixtures using targeted transcriptomics in human HepaRG hepatocarcinoma cells. Most data points supported the assumption of dose addition, with synergistic effects observed at high concentrations of certain combinations of the test compounds.
Article
Biochemistry & Molecular Biology
Simon Kling, Benedikt Lang, Helen S. Hammer, Wael Naboulsi, Heike Sprenger, Falko Frenzel, Oliver Poetz, Michael Schwarz, Albert Braeuning, Markus F. Templin
Summary: The study analyzed hepatic zonation in mouse liver using mass spectrometry and DigiWest technique, providing insights into the differences in protein expression between periportal and perivenous hepatocytes. New zonated proteins identified included kinases and phosphatases related to growth factor-dependent signaling, as well as a large panel of cytochrome P450 enzymes mainly localized in the perivenous region. The data revealed key regulators and pathways preferentially active in either periportal or perivenous hepatocytes, with beta-catenin signaling and nuclear xeno-sensing receptors being prominent perivenous regulators, and kinase- and G-protein-dependent signaling cascades predominantly active in periportal hepatocytes.
BIOLOGICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Anne-Margarethe Enge, Heike Sprenger, Albert Braeuning, Stefanie Hessel-Pras
Summary: This study investigated the mode of action of the hepatotoxic PAs component Senecionine (Sc) and found target genes and miRNAs associated with apoptosis and cellular death. The results showed a significant correlation between PA-induced miRNA-4434 induction and PAK1 downregulation. This reveals a new function of Sc-mediated toxicity in human liver cells, related to cell cycle arrest.
Article
Biochemistry & Molecular Biology
Katrin Kreuzer, Heike Sprenger, Albert Braeuning
Summary: Transcript signatures are a promising approach to identify and classify genotoxic and non-genotoxic compounds. This study analyzed transcriptomic responses in the human TK6 cell line and compared them with the HepaRG model, revealing considerable differences in the transcriptional responses between different cell models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Toxicology
Heike Sprenger, Josef Daniel Rasinger, Helen Hammer, Wael Naboulsi, Elke Zabinsky, Hannes Planatscher, Michael Schwarz, Oliver Poetz, Albert Braeuning
Summary: This study used a mouse model with humanized livers to investigate the effects of the model CAR activator phenobarbital (PB) on human and mouse hepatocytes. The proteomic analysis showed similar responses in nuclear receptor activation and xenobiotic metabolism between human and mouse hepatocytes. However, activation of the MYC proliferation regulator was only predicted in mouse hepatocytes, not in human hepatocytes. This study provides comprehensive proteomic evidence that PB induces adaptive metabolic responses but not hepatocellular proliferation in human hepatocytes in vivo.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Food Science & Technology
Heike Sprenger, Katrin Kreuzer, Jimmy Alarcan, Kristin Herrmann, Julia Buchmueller, Philip Marx-Stoelting, Albert Braeuning
Summary: Toxicological risk assessment is crucial for evaluating and authorizing chemical substances. This study investigated the genotoxic properties of three pesticidal active substances using a combination of transcriptomic data and in vitro tests with human cells. The results demonstrate the potential of transcriptomics and in vitro assays in identifying genotoxic compounds.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Biochemical Research Methods
Sam De Meyer, Daniel Felipe Cruz, Tom De Swaef, Peter Lootens, Jolien De Block, Kevin Bird, Heike Sprenger, Michael Van de Voorde, Stijn Hawinkel, Tom Van Hautegem, Dirk Inze, Hilde Nelissen, Isabel Roldan-Ruiz, Steven Maere
Summary: In this study, the authors developed a strategy for studying plant traits directly in the field, bypassing the translation step from lab experiments. They found that gene expression in autumn can predict yield traits in spring for rapeseed plants. The top predictor genes are involved in developmental processes that occur in autumn, indicating the influence of autumnal development on crop yield.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Vincent Blaschke, Alea Berten, Heike Sprenger, Jutta Zagon, Matthias Winkel
Summary: In this study, highly specific real-time PCR methods were developed for the comprehensive detection of all commercially relevant cephalopod species and the gastropod families Helicidae, Buccinidae, and Muricidae in food matrices. The specificity of the systems was demonstrated by cross-testing over 100 animal and plant species. The robustness and practical applicability of the protocol were confirmed through testing multiple parameters and cooking and autoclaving of samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Stien Mertens, Lennart Verbraeken, Heike Sprenger, Sam De Meyer, Kirin Demuynck, Bernard Cannoot, Julie Merchie, Jolien De Block, Jonathan T. Vogel, Wesley Bruce, Hilde Nelissen, Steven Maere, Dirk Inze, Nathalie Wuyts
Summary: This study evaluates the transferability of commonly used thermography analysis protocols from the field to greenhouse phenotyping platforms and finds that they can be used to monitor drought stress and develop transpiration rate prediction models in an indoor setup. The study also demonstrates that combining thermal infrared with hyperspectral imaging can further improve prediction accuracy.
