Article
Microbiology
Olga Martzoukou, Panayiotis D. Glekas, Margaritis Avgeris, Diomi Mamma, Andreas Scorilas, Dimitris Kekos, Sotiris Amillis, Dimitris G. Hatzinikolaou
Summary: In this study, researchers performed precise genome editing on the model biocatalyst Rhodococcus qingshengii IGTS8 for the first time, shedding light on the regulation of dsz gene expression and biocatalyst activity dependent on the presence of two reverse transsulfuration enzymes, C beta S and MetB. Additionally, they observed an enhancement of biodesulfurization capability in the presence of otherwise repressive sulfur sources like sulfate and L-methionine. The interconnection of cellular sulfur assimilation strategies was revealed and validated.
Article
Multidisciplinary Sciences
Jeewan Pandey, Dalton Thompson, Madhumita Joshi, Douglas C. Scheuring, Jeffrey W. Koym, Vijay Joshi, M. Isabel Vales
Summary: This study assessed the variation in free amino acid content in tetraploid potato clones and discovered genetic traits and genes associated with free amino acids. Genome-wide selection and genomic-estimated breeding values can be used to select parents and clones with desired free amino acid content in potato breeding applications.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
C. P. Sangali, E. Gasparino, A. P. Del Vesco, L. D. Castilha, L. M. D. Huepa, A. C. Furlan, A. N. T. R. Monteiro, P. C. Pozza
Summary: This study evaluated the effects of different levels of dietary methionine and vitamin B6 supplementation on finishing pigs. The results showed that higher vitamin B6 supplementation improved the feed : growth ratio, but did not effectively lower homocysteine concentration.
ANIMAL PRODUCTION SCIENCE
(2021)
Article
Geriatrics & Gerontology
Thomas M. M. Jeitner, Juan A. A. Azcona, Gene P. P. Ables, Diana Cooke, Mark C. C. Horowitz, Pradeep Singh, James M. M. Kelly, Arthur J. L. Cooper
Summary: Dietary methionine restriction (MR) prolongs lifespan by improving health. MR decreases cystathionine beta-synthase activity, leading to decreased tissue cysteine levels, but increased H2S production through beta-elimination of cysteine. MR also increases cystathionine gamma-lyase production and activities, which catabolizes cystine to form cysteine persulfide and cysteine. Cysteine inhibits cystathionine gamma-lyase non-competitively by reacting with its cofactor, limiting its function as a substrate for beta-elimination.
Article
Biochemistry & Molecular Biology
Yuchen Zhang, Qingyu Wang, Yajing Liu, Shuo Dong, Yongqiang Zhang, Youcheng Zhu, Yu Tian, Jingwen Li, Zhuoyi Wang, Ying Wang, Fan Yan
Summary: Genetically modifying soybeans to increase the content of the sulfur-containing amino acid methionine can improve the nutritional quality of soybean protein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Multidisciplinary Sciences
Heng Zhang, Yaqian Huang, Selena Chen, Chaoshu Tang, Guang Wang, Junbao Du, Hongfang Jin
Summary: H2S plays a crucial role in glucose metabolism by affecting insulin secretion, apoptosis of islet β cells, insulin sensitivity, glucose uptake, adipogenesis, lipolysis, and modulation of circadian-clock genes. Further studies are needed to determine whether H2S could be a promising target for diabetes mellitus treatment.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Maria Romerowicz-Misielak, Katarzyna Koziol, Slawomir Nowak, Marek Koziorowski
Summary: Circadian clock genes play a crucial role in regulating rhythmic processes within cells and disruptions in their functioning are associated with various disorders. H2S has been identified as a key regulator of the circadian rhythm. Pharmacological inhibition of CBS and CSE affects the expression of the Per2 gene, with exposure to AOAA potentially influenced by factors other than CBS and CSE activity.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Nutrition & Dietetics
Jiaxiang Duan, Lunli Xiang, Zhen Yang, Li Chen, Jianteng Gu, Kaizhi Lu, Daqing Ma, Hailin Zhao, Bin Yi, Hongwen Zhao, Jiaolin Ning
Summary: This study found that methionine restriction (MR) can attenuate lipopolysaccharide (LPS)-induced lung injury by regulating cystathionine-gamma-lyase (CSE) expression and hydrogen sulfide (H2S). MR inhibits the activation of TLR4/NF-kappa B/NOD-like receptor protein 3 (NLRP3) pathway, reducing the release of inflammatory mediators and immune cell infiltration.
Article
Cell Biology
Giulia Pozzi, Giuliana Gobbi, Elena Masselli, Cecilia Carubbi, Valentina Presta, Luca Ambrosini, Marco Vitale, Prisco Mirandola
Summary: T cell-mediated adaptive immunity responds to non-self antigens and pathogens by activating and proliferating various T cell populations, with hydrogen sulfide playing a crucial regulatory role in this process.
Article
Biochemistry & Molecular Biology
Yingying Wang, Xiang Ni, Rahuljeet Chadha, Caitlin McCartney, Yannie Lam, Brock Brummett, Geat Ramush, Ming Xian
Summary: Hydrogen sulfide (H2S) plays critical roles in redox biology, and its imbalance can lead to pathological processes. Recent advances in inhibitors and scavengers for H2S have been made, but challenges remain, including potency and specificity of inhibitors, as well as biologically active by-products produced during scavenging. Further development is needed for potent and specific inhibitors, as well as scavengers that generate biocompatible by-products for potential therapeutic applications.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Endocrinology & Metabolism
Kristen Skvorak, Vesna Mitchell, Leann Teadt, Kierra A. Franklin, Hyung-Ok Lee, Nikki Kruse, Callie Huitt-Roehl, Julie Hang, Faye Du, Stephanie Galanie, Steven Guan, Hera Aijaz, Nianliu Zhang, Gabriel Rajkovic, Warren D. Kruger, Moulay Hicham Alaoui Ismaili, Gjalt Huisman, Kerryn McCluskie, Adam P. Silverman
Summary: Classical homocystinuria (HCU), a rare inborn error of amino acid metabolism, is characterized by accumulation of homocysteine, leading to systemic toxicities. CDX-6512, an oral enzyme therapy, shows potential in degrading methionine and maintaining healthy homocysteine levels in preclinical studies using animal models of HCU.
MOLECULAR GENETICS AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Qamarul Hafiz Zainol Abidin, Tomoaki Ida, Masanobu Morita, Tetsuro Matsunaga, Akira Nishimura, Minkyung Jung, Naim Hassan, Tsuyoshi Takata, Isao Ishii, Warren Kruger, Rui Wang, Hozumi Motohashi, Masato Tsutsui, Takaaki Akaike
Summary: Reactive sulfur species, such as cysteine hydropersulfide and glutathione persulfide, are produced abundantly in both prokaryotes and eukaryotes, including mammals. Cysteinyl-tRNA synthetase (CARS) has been identified as a new cysteine persulfide synthase (CPERS) responsible for the production of most reactive persulfides. However, the contribution of other enzymes, such as 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine beta-synthase (CBS), and cystathionine gamma-lyase (CSE), to reactive persulfide production is still debated. In this study, the authors used sulfur metabolome analysis to demonstrate that 3-MST, CBS, and CSE are not major sources of reactive persulfides in mammals, and CARS/CPERS is the principal enzyme involved.
Article
Biochemistry & Molecular Biology
Tetsuya Miyamoto, Yasuaki Saitoh, Masumi Katane, Masae Sekine, Kumiko Sakai-Kato, Hiroshi Homma
Summary: In this study, we evaluated the enzymatic activities of human cystathionine gamma-lyase and found that it showed weak racemase activity towards various amino acids but strong lyase and dehydratase activities towards L-cystathionine and L-homoserine, respectively. The pH and temperature profiles of lyase activity were distinct from those of dehydratase activity, and lyase activity exhibited higher catalytic efficiency.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Rong-Hsuan Wang, Yu-Hsin Chu, Kai-Ti Lin
Summary: Hydrogen sulfide, while important for physiological functions, is also associated with aberrant expression in cancer development; understanding the roles of tumor-derived H2S in cancer provides opportunities for targeted therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Rodney E. Shackelford, Yan Li, Ghali E. Ghali, Christopher G. Kevil
Summary: Hydrogen sulfide (H2S) plays a significant role in DNA repair by modulating cell cycle checkpoints, DNA damage response, and genomic stability. DNA repair proteins are influenced by cellular H2S concentrations and sulfur metabolism.
Article
Multidisciplinary Sciences
Spoorthi Poreddy, Sirsha Mitra, Matthias Schoettner, Jima Chandran, Bernd Schneider, Ian T. Baldwin, Pavan Kumar, Sagar S. Pandit
NATURE COMMUNICATIONS
(2015)
Article
Multidisciplinary Sciences
Yana Aizenberg-Gershtein, Ido Izhaki, Rakesh Santhanam, Pavan Kumar, Ian T. Baldwin, Malka Halpern
SCIENTIFIC REPORTS
(2015)
Article
Ecology
Pavan Kumar, Erandi Vargas Ortiz, Etzel Garrido, Katja Poveda, Georg Jander
Article
Genetics & Heredity
Vaijayanti A. Tamhane, Ashok P. Giri, Pavan Kumar, Vidya S. Gupta
Article
Multidisciplinary Sciences
Pavan Kumar, Sagar Subhash Pandit, Ian T. Baldwin
Article
Multidisciplinary Sciences
Pavan Kumar, Preeti Rathi, Matthias Schoettner, Ian T. Baldwin, Sagar Pandit
Article
Multidisciplinary Sciences
Pavan Kumar, Sagar S. Pandit, Anke Steppuhn, Ian T. Baldwin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Food Science & Technology
Maria Teresa Fernandez-Luna, Pavan Kumar, David G. Hall, Ashaki D. Mitchell, Michael B. Blackburn, Bryony C. Bonning
Article
Biology
Tobias Zuest, Susan R. Strickler, Adrian F. Powell, Makenzie E. Mabry, Hong An, Mahdieh Mirzaei, Thomas York, Cynthia K. Holland, Pavan Kumar, Matthias Erb, Georg Petschenka, Jose-Maria Gomez, Francisco Perfectti, Caroline Mueller, J. Chris Pires, Lukas A. Mueller, Georg Jander
Article
Biochemistry & Molecular Biology
Martin L. Alani, Gordon C. Younkin, Mahdieh Mirzaei, Pavan Kumar, Georg Jander
Summary: The leaves of E. cheiranthoides are the site of cardenolide biosynthesis, with the seed and root cardenolide profiles determined by the maternal genotype, and the root cardenolide profile determined by the aboveground plant genotype.