Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 65, Issue 13, Pages 2751-2759Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b00377
Keywords
Camellia sinensis; galactinol synthase; biofunctional investigation; gene regulation; abiotic stress; biotic stress
Funding
- National Natural Science Foundation of China [31171608, 31671949]
- Special Innovative Province Construction in Anhui Province [15czs08032, 16czs08004]
- Central Guiding the Science and Technology Development of the Local [2016080503B024]
- Anhui Natural Science Foundation [1608085J08, 1608085QC57]
- Project of Universities Leading Talent Team of Nutrition and Safety of Agricultural Products in Anhui Province
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Galactinol synthase (GoIS) is a key biocatalyst for the synthesis of raffinose family oligosaccharides (RFOs). RFOs, accumulation plays a critical role in abiotic stress adaptation, but the relationship between expression of GoIS genes and biotic stress adaptation remains unclear. In this study, two CsGolS genes were found to be highly up-regulated in a transcriptome library of Ectropic oblique-attacked,Camellia sinensis. Three complete CsGolS genes were then cloned and characterized. Gene transcriptional analyses undet biotic and, abiotic stress conditions indicated that the CsGolS1 gene was sensitive to water deficit, low temperature,. and abscisic acid, while CsGolS2 and: CsGolS3 genes were sensitive to pest attack and phytohorrriones. The gene regulation and RFOs determination results indicated that CsGolS1 was primarily related to abiotic stress and CsGolS2 and CsGolS3 were related to biotic stress. GolS-mediated biotic stress adaptations have not been studied in depth, so further analysis of this new biological function is required.
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