Article
Food Science & Technology
Ruiyang Ma, Na Miao, Qingyi Zhang, Shujuan Jiang, Xinling Li, Lei Chi, Yue Li, Guangqing Mu, Xuemei Zhu
Summary: The experiment used a top-down method to quickly and efficiently screen strains with low allergenic potential and prepared fermented milk. Through direct screening of traditional fermented dairy products from Xinjiang, the BZ-21 sample with the lowest antigenicity was selected and 13 strains were isolated from it. These strains were safe and the fermented milk prepared under optimized fermentation conditions showed reduced antigenicity and IgE-binding capacity. The top-down screening method used in this experiment was effective and these 13 strains will be potential probiotics in the development of low-allergenic dairy products.
Article
Food Science & Technology
Kungnang Bunsroem, Witoon Prinyawiwatkul, Siwatt Thaiudom
Summary: This study evaluated the susceptibility of whey proteins to enzyme hydrolysis and the antidiabetic properties of protein hydrolysates. The results showed that alpha-LA hydrolysate was more effective than beta-LG hydrolysate in inhibiting DPP-W activity and increasing GLP-1 levels. Heat treatment at 75 degrees C had the greatest impact on the antidiabetic properties of the protein hydrolysates.
Article
Biochemistry & Molecular Biology
Yang Sui, Rui Jiang, Manabu Niimi, Jingru Hong, Qiaojing Yan, Zhuheng Shi, Jian Yao
Summary: Thiol antioxidants, particularly those found in albumin, have a crucial role in protecting cells and the body from oxidative stress. Recent research has demonstrated that the reductive modification of thiol residues in albumin enhances its antioxidative activity. Based on the fact that whey protein contains albumin and other thiol-active proteins, it is possible to develop novel thiol antioxidants using whey protein.
Article
Chemistry, Organic
Greg A. Erickson, Mark A. Hatcher, Michel Journet, John A. Kowalski, Tom C. Lovelace, Christopher J. Pink, Shiping Xie
Summary: A novel oxidative activation method was developed to synthesize methyltriazolo [1,4] benzodiazepine in a single step. The method has mild reaction conditions and delivers good yields. It can also be applicable to the synthesis of alkyltriazoles from other thiolactams and acylhydrazines.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Food Science & Technology
Yuanyuan Zhang, Yingcong Lu, Yang Yang, Siyao Li, Ce Wang, Cuina Wang, Tiehua Zhang
Summary: In this study, non-covalent binding interactions between three whey proteins and chlorogenic acid were investigated using spectroscopic analysis and molecular docking. The results revealed the contribution of hydrophobic force, different binding affinities and energy transfer probabilities among the proteins. The modeling and docking results suggested the involvement of hydrophobic interaction and hydrogen bond in the formation of molecular nano complexes.
Article
Chemistry, Applied
Weikai Chen, Chen Chao, Jinglin Yu, Les Copeland, Shuo Wang, Shujun Wang
Summary: Increasing the concentration of beta-lactoglobulin (β-LG) from 25 to 200 mg was found to increase the amount of ternary starch-LA-β-LG complexes formed. However, there was little difference in the amounts of the ternary complexes formed when the amount of β-LG was between 25-150 mg. Prior interaction between β-LG and lauric acid (LA) did not significantly impact the formation of ternary starch-LA-β-LG complexes.
Article
Chemistry, Inorganic & Nuclear
Hiroaki Isago, Harumi Fujita, Suzuko Nakai, Tamotsu Sugimori
Summary: Water-soluble silver(II)-phthalocyanine complexes have been synthesized and can be effectively disaggregated into monomeric species with appropriate surfactants. The complexes can rapidly react with thiols and release the corresponding macrocyclic ligand.
INORGANIC CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Vladimir I. Muronetz, Maria V. Medvedeva, Irina A. Sevostyanova, Elena V. Schmalhausen
Summary: This review focuses on the consequences of GAPDH S-nitrosylation at the catalytic cysteine residue, and discusses the hypothesis of apoptosis induction. However, there are several conflicting and unproven elements in this hypothesis.
Article
Biochemistry & Molecular Biology
Shuwen Jiang, Per Hagglund, Luke Carroll, Lars M. Rasmussen, Michael J. Davies
Summary: Different nature and extent of oxidant exposure can lead to varying levels of protein-protein crosslinks, which can be reversed by dithiothreitol and tris(2-carboxyethyl) phosphine hydrochloride. These data suggest that oxidation of disulfide bonds in proteins may result in novel inter-protein crosslinks, potentially explaining the accumulation of crosslinked proteins in aged and diseased tissues.
Article
Chemistry, Multidisciplinary
Rosalinda Mazzei, Anna Maria Szymczak, Enrico Drioli, Mohamed Al-Fageeh, Mohammed A. Aljohi, Lidietta Giorno
Summary: This study systematically investigated the separation of alpha-lactalbumin and beta-lactoglobulin using charged ultrafiltration membranes, achieving high purity (95%) and recovery factor (80%) in a single step. By operating far from the proteins' isoelectric point, protein aggregation and membrane fouling were minimized.
APPLIED SCIENCES-BASEL
(2021)
Article
Food Science & Technology
Yuan Cheng, Yuan Chi, Xuhao Geng, Yujie Chi
Summary: This study investigated the impact of protein oxidation on the physicochemical properties and digestibility of egg white protein (EWP). Results showed that increasing concentration of AAPH led to decreased amino acid content, protein solubility, and free sulfhydryl content in EWP, while carbonyl content doubled. The study also found that essential amino acids were particularly sensitive to oxidation, with a decrease of more than 10% after EWP oxidation by 5.0 mmol/L AAPH. Additionally, the formation of larger soluble aggregates by AAPH increased, and the digestibility of EWP decreased with higher oxidation degree, indicating reduced nutritional and biological activity of EWP.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Yu-Zhe Gao, Hong-Hua Xu, Chen Guan, Rui-Chi Guo, Xiao-Tong Yang, Cai-Hong Ma, Mei-Li Shao
Summary: This study found that monomers, homogeneous nuclei, and protofibrils have different abilities to resist disturbance by other protein agents during beta-lactoglobulin fibril formation. Coassembly of beta-lg monomers with α-lactalbumin/κ-casein affected fibril formation differently compared to coassembly with homogeneous nuclei and protofibrils. α-lactalbumin increased fibril diameter, while κ-casein clearly inhibited fibril formation.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Article
Agriculture, Dairy & Animal Science
Kamila Puppel, Patrycja Staniszewska, Marcin Golebiewski, Jan Slosarz, Grzegorz Grodkowski, Pawel Solarczyk, Malgorzata Kunowska-Slosarz, Piotr Kostusiak, Beata Kuczynska, Tomasz Przysucha
Summary: This study demonstrates a relationship between whey protein concentrations and blood BHBA levels, serving as markers for diagnosing metabolic diseases. Differences in alpha-lactoalbumin and beta-lactoglobulin concentrations in milk were observed based on varying BHBA levels in cows.
Article
Chemistry, Applied
Tai Zhang, Yisuo Liu, Pengjie Wang, Yixuan Li, Fazheng Ren, Huaxi Yi
Summary: The interaction between alpha-La and beta-Lg promotes the formation of aggregates in dUHT milk during heat treatment and storage, which is important for optimizing the process and storage of dUHT milk.
FOOD HYDROCOLLOIDS
(2023)
Article
Food Science & Technology
Maria Romo, Massimo Castellari, Dinar Fartdinov, Xavier Felipe
Summary: In this study, high-pressure processing was used to remove beta-Lactoglobulin from bovine native whey concentrate and generate an enriched alpha-Lactalbumin fraction. The optimal conditions for alpha-Lactalbumin yield and purification degree were determined. The results showed that high-pressure processing could improve the precipitation of beta-Lactoglobulin and the purification of alpha-Lactalbumin. Further characterization and application of membrane concentration and high-pressure processing are recommended for the valorization of cheese whey.
Article
Biochemistry & Molecular Biology
Laura Doblas, Per M. Hagglund, Eduardo Fuentes-Lemus, Michael J. Davies
Summary: This study investigates the modifications of proteins during milk processing and storage, with a focus on oxidation and crosslinking. The research reveals the formation and stability of crosslinks generated by the addition of Cys residues to quinones produced from the oxidation of Tyr residues. The findings provide insights into a novel mechanism of protein crosslink formation and information on the stability of these species, which could serve as markers for protein quality.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Campolo, Mauricio Mastrogiovanni, Michele Mariotti, Federico M. Issoglio, Dario Estrin, Per Hagglund, Tilman Grune, Michael J. Davies, Silvina Bartesaghi, Rafael Radi
Summary: Glutamine synthetase (GS) is an important enzyme that plays a crucial role in nitrogen metabolism. This study investigated the oxidative inactivation of human GS by peroxynitrite (ONOO-) in vitro. The results showed that ONOO- exposure led to a dose-dependent loss of GS activity and various oxidative modifications of the enzyme. Multiple tyrosine nitration sites and dityrosine cross-links were identified, but nitration of specific tyrosine residues alone was not responsible for enzyme inactivation. In addition, ONOO- induced GS aggregation and activity loss, with thiol oxidation being a key modification to elicit aggregation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Max B. Sauerland, Christina Helm, Lasse G. Lorentzen, Asmita Manandhar, Trond Ulven, Luke F. Gamon, Michael J. Davies
Summary: Alpha, beta-unsaturated carbonyls are commonly found in environmental toxins and therapeutic drugs. However, the specific targets and mechanisms of these compounds are poorly understood. Recently, a click-chemistry method has been developed to visualize and enrich adducted proteins for further characterization. LC-MS proteomic analysis has identified the cellular targets of DMFU and MMFU.
Article
Biochemistry & Molecular Biology
Yihe Wang, Astrid Hammer, Gerald Hoefler, Ernst Malle, Clare L. Hawkins, Christine Y. Chuang, Michael J. Davies
Summary: Atherosclerosis is a chronic inflammatory disease characterized by plaque formation in arterial walls. The study found that hypochlorous acid (HOCl) and chloramines can specifically modify aggrecan, a proteoglycan involved in atherogenesis. These modifications, different from those induced by ADAMTS1, can lead to irreversible protein cross-links. The findings also showed colocalization of aggrecan and HOCl-generated epitopes in advanced human atherosclerotic plaques.
Article
Biochemistry & Molecular Biology
Jianfei He, Christine Y. Chuang, Clare L. Hawkins, Michael J. Davies, Per Hagglund
Summary: The extracellular matrix (ECM) of tissues is formed by proteins, proteoglycans, and glycosaminoglycans, and it plays a crucial role in maintaining tissue integrity. Peroxynitrite, an oxidant produced during inflammation, can modify fibronectin and impair its function. This study investigated the effects of peroxynitrite on the structure of ECM and its interactions with cellular components. The results suggest that peroxynitrite can influence ECM structure and may have implications in pathological conditions such as atherosclerosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Qing Gao, Katarzyna Grzyb, Luke F. Gamon, Peter R. Ogilby, Tomasz Pedzinski, Michael J. Davies
Summary: Disulfide bonds are critical for protein structure and function, and their modification can lead to loss of structure and function. This study investigates the oxidation of disulfide bonds by singlet oxygen, showing selective oxidation and variation in reaction rates and product formation. These findings suggest that certain disulfides, including those in proteins, may undergo detrimental modifications that affect protein structure and function.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Juan Sebastian Reyes, Eduardo Fuentes-Lemus, Jefferson Romero, Felipe Arenas, Angelica Fierro, Michael J. Davies, Camilo Lopez-Alarcon
Summary: 6-phosphogluconolactonase (6PGL) is an enzyme involved in the pentose phosphate pathway (PPP) and is susceptible to oxidative inactivation. This study investigates the oxidation of Escherichia coli 6PGL by peroxyl radicals and its effect on enzymatic activity and NADPH generation. The results show that while monomeric 6PGL is resistant to oxidative inactivation, protein aggregation and oxidation of specific residues occur, leading to diminished NADPH generation. These findings contribute to our understanding of the oxidative stability of 6PGL compared to other PPP enzymes.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Guilherme T. Voss, Michael J. Davies, Carl H. Schiesser, Renata L. de Oliveira, Andresa B. Nornberg, Angelita M. Barcellos, Cristiane Luchese, Ethel A. Wilhelm
Summary: This study investigated the use of 1,4-anhydro-4-seleno-D-talitol (SeTal) in gelatin and alginate (Gel-Alg) polymeric films to improve the treatment and attenuation of atopic dermatitis (AD)-like symptoms in mice. The films were able to retain and release SeTal in a controlled manner and effectively attenuated disease symptoms. Moreover, the loaded films showed superior efficiency compared to hydrocortisone (HC) cream, a traditional AD treatment. Overall, incorporating SeTal in biopolymeric films provides a promising alternative for the long-term treatment of AD-type skin diseases.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Physical
Angie C. Forero-Giron, Denis Fuentealba, Nory Marino-Ocampo, Soledad Gutierrez-Oliva, Barbara Herrera, Alejandro Toro-Labbe, Eduardo Fuentes-Lemus, Michael J. Davies, Margarita E. Aliaga, Camilo Lopez-Alarcon
Summary: The stable inclusion complex of AAPH with CB7 can generate high yields of peroxyl radicals, making it useful for studying these species in various fields such as material, environmental, and biomedical sciences.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biochemistry & Molecular Biology
Joao M. Anghinoni, Paloma T. Birmann, Marcia J. da Rocha, Caroline S. Gomes, Michael J. Davies, Cesar A. Bruening, Lucielli Savegnago, Eder J. Lenardao
Summary: In this review, the synthesis and bioassays of organic selenium compounds with antioxidant activity in the past five years are discussed, presenting the most active compounds in each series.
Article
Pharmacology & Pharmacy
Raffaella di Vito, Mattia Acito, Cristina Fatigoni, Carl H. Schiesser, Michael J. Davies, Francesca Mangiavacchi, Milena Villarini, Claudio Santi, Massimo Moretti
Summary: This study evaluated the cytotoxic and genotoxic potential of SeTal in liver cells through in vitro experiments. The results showed that SeTal had minimal impact on cell viability and did not cause clastogenic or aneugenic activity. These findings suggest that SeTal may be a potential active pharmaceutical ingredient.
Article
Biochemistry & Molecular Biology
Ramona Clemen, Eduardo Fuentes-Lemus, Sander Bekeschus, Michael J. Davies
Summary: Diabetes is a complex disease with increasing prevalence. Chronic inflammation, induced by oxidants, leads to protein modifications and functional changes. The effects of oxidants on amylin aggregation and function are still unclear.
Article
Biochemistry & Molecular Biology
Sara M. Jorgensen, Lasse G. Lorentzen, Astrid Hammer, Gerald Hoefler, Ernst Malle, Christine Y. Chuang, Michael J. Davies
Summary: Continued oxidant production during chronic inflammation leads to tissue damage, which is associated with atherosclerosis. Versican, an ECM proteoglycan, accumulates during atherogenesis and promotes inflammation. We hypothesized that versican is targeted by oxidants and undergoes modifications that aggravate plaque development.
Article
Biochemistry & Molecular Biology
Shuqi Xu, Christine Y. Chuang, Clare L. Hawkins, Per Hagglund, Michael J. Davies
Summary: Peroxynitrous acid/peroxynitrite (ONOOH/ONOO ¡) is a powerful oxidizing/nitrating system formed at sites of inflammation, which can modify biological targets, particularly proteins. This study found that multiple proteins from primary human coronary artery smooth muscle cells are nitrated, with various modifications occurring on cellular and extracellular matrix proteins. The nitration occurs both endogenously and exogenously, and it may have significant effects on cell and protein function, potentially contributing to the development and exacerbation of diseases such as atherosclerosis.
Article
Microbiology
Iuliana Popa, David Touboul, Tilde Andersson, Eduardo Fuentes-Lemus, Cyrille Santerre, Michael J. Davies, Rolf Lood
Summary: Cutibacterium acnes, an abundant bacteria on the skin, relies on the secretion of its antioxidant protein RoxP to maintain skin health. The oxidation status of proteins and the composition of lipids in C. acnes are influenced by environmental oxidative conditions and the presence of the bacterial antioxidant system.