Nakazawaea todaengensis f.a., sp nov., a yeast isolated from a peat swamp forest in Thailand

Strain DMKU-PS11(1)(T) was isolated from peat in a swamp forest in Thailand. DNA sequence analysis showed that it belonged to a novel species that was most closely related to Nakazawaea laoshanensis. However, it differed from the type strain of N. laoshanensis (NRRL Y-63634(T)) by 2.3% nucleotide substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene, 1.0% nucleotide substitutions in the small subunit (SSU) rRNA gene and 8.0% nucleotide substitutions in the internal transcribed spacer (ITS) region. The phylogenetic analyses based on the combined sequences of the SSU and the D1/D2 region and that of the SSU sequences alone confirmed the placement of the novel species in the Nakazawaea clade and its close affinity with N. laoshanensis. Hence, the species Nakazawaea todaengensis f.a., sp. nov. is proposed. The type strain is DMKU-PS11(1)(T) (=CBS 14555(T)=TBRC 6559(T)). The MycoBank number for Nakazawaea todaengensis f.a., sp. nov. is MB 819513