Algoriphagus aestuariicola sp nov., isolated from estuary sediment

A Gram-staining-negative and strictly aerobic bacterium, designated strain S2-A1(T), was isolated from estuary sediment in South Korea. Cells of strain S2-A1(T) were oxidase-and catalase-positive rods without a gliding motility. Growth was observed at 15-40 degrees C (optimum, 37 degrees C), at pH 6.0-10.0 (optimum, pH 7.0-7.5) and in the presence of 0-4.0% (w/v) NaCl (optimum, 0.5-1.0 %). The sole respiratory quinone was MK-7. iso-C-15:(0), summed feature 3 (comprising C-16:1 omega 7c/C-16:1 omega 6c)and summedfeature 9 (comprising iso-C-17:(1 omega)9c/C-16:(0) 10-methyl) were found as the major fatty acids (>5% of the total fatty acids). The polar lipids of strain S2-A1(T) consisted of phosphatidylethanolamine, an unidentified aminophospholipid, three unidentified aminolipids and five unidentified lipids. The G+C content of the genomic DNA was 45.6 mol%. Strain S2-A1(T) was most closely related to Algoriphagus taeanensis HMC4223(T) with a 97.7% 16S rRNA gene sequence similarity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S2-A1(T) formed a tight phyletic lineage with members of the genus Algoriphagus. On the basis of phenotypic, chemotaxonomic and molecular features, strain S2-A1(T) clearly represents a novel species of the genus Algoriphagus, for which the name Algoriphagus aestuariicola sp. nov. is proposed. The type strain is S2A1(T) (= KACC 18987(T) = JCM 31546(T)).