Article
Biotechnology & Applied Microbiology
Isabelle Heker, Gebhard Haberhauer, Rainer U. U. Meckenstock
Summary: Pollution with polycyclic aromatic hydrocarbons is a significant threat to humans and animals with long-term effects. The mechanism of naphthalene carboxylase, a key enzyme in anaerobic naphthalene degradation, was investigated in this study. The proposed mechanism involves three steps: activation of naphthalene, release of a proton and rearomatization, and carboxylation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Environmental Sciences
Silviu-Laurentiu Badea, Violeta -Carolina Niculescu, Diana-Ionela Popescu (Stegarus), Elisabeta-Irina Geana, Corina-Teodora Ciucure, Oana-Romina Botoran, Roxana-Elena Ionete
Summary: Compound specific isotope analysis has been extensively used to study the environmental processes associated with the transformation of persistent halogenated organic pollutants. Recent advancements have expanded its application to larger molecules and multi-element analysis. However, the instrumental detection limit remains challenging, especially for δ13C analysis, and liquid chromatography-combustion isotope ratio mass spectrometry methods face difficulties in complex mixture analysis. Alternative approaches, such as enantioselective stable isotope analysis, have been explored for chiral contaminants, but more research is needed. In order to analyze new emerging halogenated organic contaminants, there is a need to develop new GC and LC methods for non-target screening using high resolution mass spectrometry prior to compound specific isotope analysis (CSIA) methods.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Alice Delacuvellerie, Samira Benali, Valentine Cyriaque, Sebastien Moins, Jean-Marie Raquez, Sylvie Gobert, Ruddy Wattiez
Summary: The study compared the biodegradation of compostable and non-compostable plastics in the natural marine environment, finding differences in bacterial degradation of different plastics and providing important insights for further scientific research.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Hao Li, Jun Yao, Ning Min, Geoffrey Sunahara, Jianli Liu, Miaomaio Li, Bang Liu, Wancheng Pang, Ying Cao, Ruofei Li, Robert Duran
Summary: Many mining and smelting activities have resulted in severe metal(loid) contamination in local soil environments. This study investigated the metabolic activity of soil microorganisms in four areas affected by different metallurgical activities. It was found that the activities of soil microorganisms were correlated with the metal(loid) properties. The study provides a theoretical basis for ecological prevention and control of metal-polluted areas.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Xiangkun Kong, Na Lv, Songmeng Liu, Hui Xu, Junwei Huang, Xiangting Xie, Qing Tao, Baozhan Wang, Rong Ji, Qun Zhang, Jiandong Jiang
Summary: In this study, the bacterial N-demethylase gene pdmAB from isoproturon (IPU) was successfully transferred and expressed in soybeans. The transgenic soybeans showed improved tolerance to IPU and efficiently converted it to a less toxic metabolite. They also demonstrated high removal efficiency of IPU from water and soil, while maintaining a better symbiotic nitrogen fixation capacity and stable rhizosphere bacterial community under IPU stress.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Environmental Sciences
T. S. Rejiniemon, R. Lekshmi, Hissah Abdulrahman Alodaini, Ashraf Atef Hatamleh, Rengasamy Sathya, Palaniselvam Kuppusamy, Munirah Abdullah Al-Dosary, M. Kalaiyarasi
Summary: This study screened three bacterial strains capable of degrading naphthalene (NAP) using an enrichment culture method. The identified strains were Bacillus cereus CK1, Pseudomonas aeruginosa KD4, and Enterobacter aerogenes SR6. The bacterial consortium demonstrated efficient NAP degradation under optimal conditions, highlighting its potential for removing polycyclic aromatic hydrocarbons (PAHs).
Article
Biotechnology & Applied Microbiology
Kartik Dhar, Logeshwaran Panneerselvan, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Summary: Bioaugmentation effectively enhances microbial bioremediation of hazardous polycyclic aromatic hydrocarbons (PAHs) from contaminated environments. Screening for pyrene-degrading bacteria resulted in the discovery of a mixed enrichment culture that was more efficient in PAHs biodegradation than culturable pure strains. This culture was dominated by a previously uncultured member of the family Rhizobiaceae and utilized C1 and other methylotrophic substrates. It rapidly degraded PAHs in liquid medium and showed good efficiency in bioremediation of PAHs in bench-scale slurry systems.
Article
Environmental Sciences
Lucelia Cabral, Patricia Giovanella, Elisa Pais Pellizzer, Elias Hideo Teramoto, Chang Hung Kiang, Lara Duraes Sette
Summary: In recent decades, hydrocarbon concentrations in soil and water have increased due to accidents or operations involving crude oil and petroleum input into the environment. Bioremediation has emerged as an effective environmental recovery approach, utilizing hydrocarbon compounds as growth substrates for microorganisms, contributing to the stabilization of impacted areas.
Article
Multidisciplinary Sciences
Muhammad Yasir, Arooj K. Qureshi, Esam Azhar
Summary: This study analyzed sediment samples from the non-volcanic Tatta Pani hot springs in Azad Kashmir using both culture-dependent and culture-independent techniques. The results showed high microbial diversity in the enriched sediment samples but adverse changes in the composition of relatively dominant bacteria. Metagenomic analyses are recommended for further study of hot spring microbiota diversity and functionality.
Article
Chemistry, Organic
Mir Sajad Rabani, Richa Sharma, Rachna Singh, Mahendra K. Gupta
Summary: Bacterial isolates obtained from petroleum-contaminated sites in Gwalior, India showed tolerance and degradation potential towards naphthalene and heavy metals, indicating their potential in environmental remediation.
POLYCYCLIC AROMATIC COMPOUNDS
(2022)
Review
Environmental Sciences
Shuai Zhang, Yingyue Han, Jingyu Peng, Yunmin Chen, Liangtong Zhan, Jinlong Li
Summary: Soil contamination is a global hazard that poses a threat to human health as contaminants can migrate through different mediums. Human Health Risk Assessment (HHRA) is a widely used method for evaluating the health effects of contaminants. However, there is a lack of comprehensive information on the methods and models of HHRA. This paper aims to review the current approaches and models of HHRA, specifically focusing on contaminated sites.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Engineering, Environmental
Lin Jiang, Ruihuan Zhang, Lina Zhang, Rui Zheng, Maosheng Zhong
Summary: A new risk assessment strategy for mercury-contaminated sites was proposed using bioaccessible fractions and soil Hg vapor concentrations. Experimental results showed a significant decrease in Hg risk for oral ingestion and inhalation pathways when using this strategy.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Green & Sustainable Science & Technology
Yefeng Jiang, Zhou Shi, Fang Xia, Zhe Xu
Summary: This study proposes a novel framework that integrates social media data and field investigations to monitor contaminated sites. The results show that social media user tweets and contaminated site numbers exhibit similar trends, and social media and field investigations can complement each other to provide support for real-time monitoring on a global scale in the future.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Environmental Sciences
Matthew C. Harwell, Chloe Jackson, Michael Kravitz, Kira Lynch, Jewel Tomasula, Anne Neale, Michele Mahoney, Carlos Pachon, Karen Scheuermann, Gregory Grissom, Kristen Parry
Summary: This article reviews advancements in the application of ecosystem services in cleaning contaminated sites in the U.S. since 2009, presenting a four-step transferable framework to assist stakeholders in considering and implementing ES concepts and guidelines.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Engineering, Environmental
Siyi Lin, Xun Wen Chen, Zongwei Cai, Jianbo Shi, Jianjie Fu, Guibin Jiang, Ming Hung Wong
Summary: The use of primitive techniques in recycling electronic waste has resulted in severe soil pollution. Remediation techniques such as spray tower, electronic precipitation, photocatalysis, and phytoremediation using suitable plants and microbes are recommended to decontaminate soil, sediment, and water contaminated by toxic metals and persistent organic pollutants. Proper vegetation can also extract, stabilize, and degrade pollutants, restoring soil functions and health.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Christopher Burot, Remi Amiraux, Patricia Bonin, Sophie Guasco, Marcel Babin, Fabien Joux, Dominique Marie, Laure Vilgrain, Hermann J. Heipieper, Jean-Francois Rontani
Summary: In this study, lipid composition and viability of bacteria in sea ice and suspended particulate matter (SPM) samples along a transect from sea ice to open water in Baffin Bay were monitored. The results revealed the presence of different phytoplankton and zooplankton species in samples collected at different locations, as well as high bacterial mortality and isomerization of bacterial MUFAs. Additionally, the study suggested the presence of EPS-rich particles as ideal vectors for bacterial horizontal distribution in the Arctic, containing a high proportion of dead and non-growing bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Biochemistry & Molecular Biology
Nora Lisa Bitzenhofer, Luzie Kruse, Stephan Thies, Benedikt Wynands, Thorsten Lechtenberg, Jakob Roenitz, Ekaterina Kozaeva, Nicolas Thilo Wirth, Christian Eberlein, Karl-Erich Jaeger, Pablo Ivan Nikel, Hermann J. Heipieper, Nick Wierckx, Anita Loeschcke
Summary: Species of the Pseudomonas clade possess multiple stress response mechanisms to cope with harmful chemicals, including built-in cell envelope stress response and effective export machineries for harmful compounds.
MICROBIAL CELL FACTORIES-BOOK
(2021)
Article
Multidisciplinary Sciences
Maria Jose Cardenas Espinosa, Tabea Schmidgall, Georg Wagner, Uwe Kappelmeyer, Stephan Schreiber, Hermann J. Heipieper, Christian Eberlein
Summary: Research on bacterial degradation of xenobiotic compounds has been ongoing for decades, with recent advancements in molecular applications such as NGS and qPCR highlighting the importance of high-quality RNA extraction from aromatic substrates. This study successfully established a method for total RNA extraction from Pseudomonas capeferrum TDA1, overcoming challenges posed by interference from polyphenolic compounds, and may serve as a guideline for improving RNA extraction from other bacterial species.
Article
Multidisciplinary Sciences
Oscar Puiggene, Maria Jose Cardenas Espinosa, Dietmar Schlosser, Stephan Thies, Nico Jehmlich, Uwe Kappelmeyer, Stephan Schreiber, Daniel Wibberg, Joern Kalinowski, Hauke Harms, Hermann J. Heipieper, Christian Eberlein
Summary: A strain of Pseudomonas capeferrum TDA1, isolated from a plastic dump site, has been found to possess the ability to degrade polyurethane (PU) compounds. The degradation pathway and mechanisms for PU degradation in this organism have been further explored.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Thibault Chautrand, Segolene Depayras, Djouhar Souak, Tatiana Kondakova, Magalie Barreau, Takfarinas Kentache, Julie Hardouin, Ali Tahrioui, Olivier Thoumire, Yoan Konto-Ghiorghi, Corinne Barbey, Guy Ladam, Sylvie Chevalier, Hermann J. Heipieper, Nicole Orange, Cecile Duclairoir-Poc
Summary: This study examines the physiological effects of NO2 on the cell envelope of the airborne bacterium Pseudomonas fluorescens MFAF76a and its potential adaptive responses. Exposure to NO2 leads to morphological alterations of the cell envelope and modifications in the synthesis pathways of multiple cell envelope components.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Sarah Riesbeck, Hannes Petruschke, Ulrike Rolle-Kampczyk, Christian Schori, Christian H. Ahrens, Christian Eberlein, Hermann J. Heipieper, Martin von Bergen, Nico Jehmlich
Summary: Bisphenols can disturb the gut microbiome and inhibit the growth of human commensal bacteria, leading to changes in physiological and functional state of cells. These findings help to better understand the impact of bisphenols on host health and disease development.
Review
Engineering, Environmental
Anna Parus, Tomasz Ciesielski, Marta Wozniak-Karczewska, Mariusz Slachcinski, Mikolaj Owsianiak, Lukasz Lawniczak, Andreas P. Loibner, Hermann J. Heipieper, Lukasz Chrzanowski
Summary: This article explores key issues regarding the use of rhamnolipids in the treatment of heavy metal and petroleum hydrocarbon-contaminated soils, including concentration, phytotoxicity, biodegradation, and interaction with soil microorganisms.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Physical
Anna Parus, Oskar Zdebelak, Tomasz Ciesielski, Radoslaw Szumski, Marta Wozniak-Karczewska, Grzegorz Framski, Daniel Baranowski, Michal Niemczak, Joanna Zembrzuska, Tomas Cajthaml, Hermann J. Heipieper, Lukasz Chrzanowski
Summary: This study investigated the stability and adsorption characteristics of different structured ILs in the soil environment. The results indicate that the stability and adsorption behavior of ILs are influenced by the properties of the cations and anions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biotechnology & Applied Microbiology
Uwe Strotmann, Gerald Thouand, Udo Pagga, Stefan Gartiser, Hermann J. Heipieper
Summary: In the past decades, a complex standardized system has been developed by the industrial and scientific communities to evaluate the biodegradability of chemical substances. Although this system has been widely adopted and integrated into European legislation, there are still deficiencies in the different tests and their accuracy in reflecting the real environment. This review focuses on the technical advantages and weaknesses of current tests, the properties of microbial inocula, and the prediction models for biodegradation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Wiktoria Wilms, Marta Wozniak Karzewska, Michal Niemczak, Anna Parus, Robert Frankowski, Lukasz Wolko, Jakub Czarny, Agnieszka Piotrowska-Cyplik, Agnieszka Zgola-Grzeskowiak, Hermann J. Heipieper, Lukasz Chrzanowski
Summary: The commercial formulations of herbicides depend on surfactants to enhance their effectiveness. Herbicidal ionic liquids (ILs), which combine cationic surfactants with herbicidal anions, offer the advantage of reducing additives and achieving good herbicide performance at lower doses. Our study investigated the impact of synthetic and natural cations on the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D). The results demonstrated that even the introduction of naturally-derived cations led to an increase in the herbicide's half-lives, indicating the need for further research on environmentally friendly compounds and a reassessment of ionic liquids as mixtures of ions in the environment.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Microbiology
Maria Jose Cardenas Espinosa, Tabea Schmidgall, Jessica Pohl, Georg Wagner, Benedikt Wynands, Nick Wierckx, Hermann J. Heipieper, Christian Eberlein
Summary: This study assessed the tolerance of two Pseudomonas strains, P. taiwanensis VLB120 and P. capeferrum TDA1, to organic solvents. The results showed that these strains had higher tolerance compared to the well-studied strain P. putida DOT-T1E and exhibited stronger adaptability. Therefore, these strains have the potential to serve as platforms for industrial production of various chemicals.
Article
Agricultural Engineering
Tobias Schwanemann, Esther A. Urban, Christian Eberlein, Jochem Gaetgens, Daniela Rago, Nicolas Krink, Pablo I. Nikel, Hermann J. Heipieper, Benedikt Wynands, Nick Wierckx
Summary: Efficient heterologous production of polyketides from (hydroxy)benzoates was achieved through whole-cell biocatalysis in combination with in situ product extraction using a biocompatible organic solvent. The choice of CoA ligase and polyketide synthase type III had a significant impact on the production. Solvent screening identified 2-undecanone as a suitable solvent with good phase separation. Two-phase cultivations with metabolically engineered strains enabled complete de novo synthesis of all (hydroxy)benzoate-derived polyketides.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nora Lisa Bitzenhofer, Carolin Hoefel, Stephan Thies, Andrea Jeanette Weiler, Christian Eberlein, Hermann J. J. Heipieper, Renu Batra-Safferling, Pia Sundermeyer, Thomas Heidler, Carsten Sachse, Tobias Busche, Jorn Kalinowski, Thomke Belthle, Thomas Drepper, Karl-Erich Jaeger, Anita Loeschcke
Summary: This study found that the formation of outer membrane vesicles (OMV) in Pseudomonas putida KT2440 is correlated with the recombinant production of the natural compound tripyrrole prodigiosin. By manipulating the expression of genes associated with OMV formation, the yields of various compounds such as prodigiosin, violacein, phenazine-1-carboxylic acid, and zeaxanthin were significantly increased.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Przemys law Bernat, Anna Jasinska, Katarzyna Niedzialkowska, Miros lawa Slaba, Sylwia Rozalska, Katarzyna Paraszkiewicz, Lidia Sas-Paszt, Hermann J. Heipieper
Summary: This study investigated the effects of the herbicide metolachlor and microplastics on the growth and adaptive responses of Trichoderma harzianum in soil. The findings showed that microplastics had a marginal inhibitory effect on the fungus, while metolachlor exhibited a dose-dependent inhibitory effect. However, the presence of microplastics did not influence the fungal degradation activity of the herbicide. Additionally, the activities of six extracellular enzymes in the soil were significantly enhanced in the presence of metolachlor.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Geosciences, Multidisciplinary
Philippe Massicotte, Rainer M. W. Amon, David Antoine, Philippe Archambault, Sergio Balzano, Simon Belanger, Ronald Benner, Dominique Boeuf, Annick Bricaud, Flavienne Bruyant, Gwenaelle Chaillou, Malik Chami, Bruno Charriere, Jing Chen, Herve Claustre, Pierre Coupel, Nicole Delsaut, David Doxaran, Jens Ehn, Cedric Fichot, Marie-Helene Forget, Pingqing Fu, Jonathan Gagnon, Nicole Garcia, Beat Gasser, Jean-Francois Ghiglione, Gaby Gorsky, Michel Gosselin, Priscillia Gourvil, Yves Gratton, Pascal Guillot, Hermann J. Heipieper, Serge Heussner, Stanford B. Hooker, Yannick Huot, Christian Jeanthon, Wade Jeffrey, Fabien Joux, Kimitaka Kawamura, Bruno Lansard, Edouard Leymarie, Heike Link, Connie Lovejoy, Claudie Marec, Dominique Marie, Johannie Martin, Jacobo Martin, Guillaume Masse, Atsushi Matsuoka, Vanessa McKague, Alexandre Mignot, William L. Miller, Juan-Carlos Miquel, Alfonso Mucci, Kaori Ono, Eva Ortega-Retuerta, Christos Panagiotopoulos, Tim Papakyriakou, Marc Picheral, Louis Prieur, Patrick Raimbault, Josephine Ras, Rick A. Reynolds, Andre Rochon, Jean-Francois Rontani, Catherine Schmechtig, Sabine Schmidt, Richard Sempere, Yuan Shen, Guisheng Song, Dariusz Stramski, Eri Tachibana, Alexandre Thirouard, Imma Tolosa, Jean-Eric Tremblay, Mickael Vaitilingom, Daniel Vaulot, Frederic Vaultier, John K. Volkman, Huixiang Xie, Guangming Zheng, Marcel Babin
Summary: The MALINA oceanographic campaign in 2009 aimed to investigate carbon stocks and fluxes in the Mackenzie River estuary and Beaufort Sea, collecting a range of physical, chemical, and biological variables across seven transects. Data standardization and compilation efforts have been made to facilitate further studies on the changing Arctic Ocean.
EARTH SYSTEM SCIENCE DATA
(2021)