Article
Engineering, Biomedical
Nastaran Shojarazavi, Shohreh Mashayekhan, Hossein Pazooki, Sadaf Mohsenifard, Hossein Baniasadi
Summary: In this study, an injectable hydrogel incorporating alginate, cartilage extracellular matrix, and silk fibroin nanofibers was developed for cartilage tissue engineering. The mechanical properties of the hydrogel were optimized by varying alginate and SFN concentrations. The optimized hydrogel showed promising characteristics for potential use as an injectable scaffold for articular cartilage defects, mimicking the natural cartilage environment.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2021)
Article
Polymer Science
Ssu-Meng Haung, Yu-Ting Lin, Shih-Ming Liu, Jian-Chih Chen, Wen-Cheng Chen
Summary: In this study, porous scaffolds made of gelatin, hyaluronic acid, alginate, and sucrose in different proportions were used for chondrocyte regeneration. The results showed that the scaffold with the highest porosity and strength did not perform the best, while another scaffold provided a more suitable environment for chondrocyte growth. Developing hydrogels with customized pore properties for defective cartilage is expected to meet the requirements of ultimate clinical applications.
Article
Polymer Science
Leila Miri, Shiva Irani, Mohamad Pezeshki-Modaress, Hamed Daemi, Seyed Mohammad Atyabi
Summary: This study focuses on the preparation of surface-modified electrospun polycaprolactone nanofibrous scaffolds with sulfated alginate to enhance the chondrogenic differentiation of mesenchymal stem cells. The results demonstrate that the sulfated alginate-modified nanofibrous scaffold can promote the differentiation of mesenchymal stem cells into chondrocytes.
Article
Biochemistry & Molecular Biology
Resmi Rajalekshmi, Anusree Kaladevi Shaji, Roy Joseph, Anugya Bhatt
Summary: The development of a tissue-engineered construct for hepatic regeneration is challenging due to the lack of an optimum environment for hepatocyte growth. Injectable alginate dialdehyde-gelatin hydrogel incorporating fibrin was explored as a matrix for liver tissue engineering, showing promising support for the growth and function of HepG2 cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Chemistry, Applied
Sumit Murab, Aastha Gupta, Malgorzata Katarzyna Wlodarczyk-Biegun, Anuj Kumar, Patrick van Rijn, Patrick Whitlock, Sung Soo Han, Garima Agrawal
Summary: This review highlights the importance of alginate-based hydrogel inks for fabricating 3D printed scaffolds for bone and cartilage regeneration. The fundamentals of direct extrusion 3D bioprinting method are discussed, and a comprehensive overview of various alginate-based hydrogel ink formulations that have been used so far is provided. The requirements of hydrogel inks and 3D printed scaffolds to achieve similarity to the native tissue environment are summarized.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Young Jun Kim, Junhong Min
Summary: By hybridizing alginate and PVA to form a semi-interpenetrating polymer network (semi-IPN), the properties of the hydrogel were successfully modulated, retaining the advantages of alginate-based hydrogels while covering their disadvantages. The hydrogel exhibited good performance in stimuli-responsive behavior, degradability, mechanical stability, and physicochemical stability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Yun Chu, Lei Huang, Wangping Hao, Tongtong Zhao, Haitao Zhao, Wen Yang, Xin Xie, Lin Qian, Yanyan Chen, Jianwu Dai
Summary: 3D bioprinting of double crosslinked alginate hydrogel promotes MSC differentiation into cartilage and demonstrates good mechanical properties, stability, and printing accuracy.
BIOMEDICAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Xavier Barcelo, Kian F. Eichholz, Orquidea Garcia, Daniel J. Kelly
Summary: The study investigated the effect of alginate oxidation on the degradation rate of bioinks, resulting in a range of degradable bioinks with the degradation rate highly dependent on the content of partially oxidized alginate. Despite high mass loss, all bioink blends except the 0:100 group maintained the initially printed geometry throughout a 4 week in vitro culture period and supported robust chondrogenic differentiation of mesenchymal stem/stromal cells.
Article
Polymer Science
Dongze Li, Yuqing Liao, Xiuqiong Chen, Hongcai Wang, Yanshi Wen, Kaiyue Cheng, Weiwei Chen, Huiqiong Yan, Qiang Lin
Summary: In this study, OSA/SiO2/PAM-GT composite hydrogels were fabricated using the interpenetrating polymer network technology and incorporating SiO2 nanoparticles. The effect of SiO2 nanoparticles on the properties of the composite hydrogels was investigated. The results showed that the addition of SiO2 nanoparticles could effectively regulate the characteristics of the composite hydrogels and provide good support for cells.
Review
Biotechnology & Applied Microbiology
Xia Zhao, Daniel A. Hu, Di Wu, Fang He, Hao Wang, Linjuan Huang, Deyao Shi, Qing Liu, Na Ni, Mikhail Pakvasa, Yongtao Zhang, Kai Fu, Kevin H. Qin, Alexander J. Li, Ofir Hagag, Eric J. Wang, Maya Sabharwal, William Wagstaff, Russell R. Reid, Michael J. Lee, Jennifer Moriatis Wolf, Mostafa El Dafrawy, Kelly Hynes, Jason Strelzow, Sherwin H. Ho, Tong-Chuan He, Aravind Athiviraham
Summary: Cartilage, especially articular cartilage, is crucial for joint function and mobility, but has limited self-repair capacity. Cartilage tissue engineering (CTE) holds promise for repair, requiring effective chondrogenic factors, ample chondrogenic progenitors, and cell-friendly, biocompatible scaffold materials for success. Progress in these areas, along with advancements in 3D bio-printing, may improve clinical outcomes of cartilage repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Promita Bhattacharjee, Mark Ahearne
Summary: There is a need to develop tissue engineering methods to address the shortage of donor corneas for transplantation. A novel approach using free-radical polymerization to fabricate three-dimensional hydrogels for corneal stromal tissue regeneration was investigated. The hydrogels showed rapid gelation, good porosity, enhanced cellular adhesion and proliferation, and no cytotoxicity. This study provides an initial step towards developing clinically applicable ocular implants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Pariya Zare, Mohamad Pezeshki-Modaress, Seyed Mohammad Davachi, Pouria Zare, Fatemeh Yazdian, Sara Simorgh, Hadi Ghanbari, Hamid Rashedi, Zohreh Bagher
Summary: In this study, two different arrangements of laminated composite scaffolds were fabricated based on Alginate, showcasing improved mechanical features and sustained drug release. The optimized scaffold displayed advantages such as interconnected pores, controlled biodegradability, and no cytotoxic effect, making it a promising candidate for tissue regeneration.
CARBOHYDRATE POLYMERS
(2021)
Article
Polymer Science
Kanyakorn Riewruja, Alyssa M. M. Aguglia, Sophie Hines, Meagan J. J. Makarcyzk, Sittisak Honsawek, Hang Lin
Summary: This study successfully enhanced the mechanical strength of gelatin/hyaluronic acid (GH) scaffolds by infiltrating polyethylene glycol diacrylate (PEGDA) and photoinitiators. The PEG-GH hybrid scaffolds displayed good integration capacity and provide a new tool for the repair of chondral injury.
Article
Biochemistry & Molecular Biology
Hsia-Wei Liu, Wen-Ta Su, Ching-Yi Liu, Ching-Cheng Huang
Summary: A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. The results showed that the scaffold has great potential in promoting chondrocyte proliferation, maintaining the structural stability of cartilage, and promoting cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Zhiyao Liao, Liwei Fu, Pinxue Li, Jiang Wu, Xun Yuan, Chao Ning, Zhengang Ding, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: A bioactive multifunctional scaffold was developed using Mg2+ and aptamer Apt19S to promote articular cartilage regeneration and early inflammatory regulation, offering a promising strategy for AC defect treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Letter
Surgery
Deepti Singh, Daniel Thomas
AMERICAN JOURNAL OF SURGERY
(2019)
Letter
Surgery
Daniel Thomas, Deepti Singh
AMERICAN JOURNAL OF SURGERY
(2019)
Article
Biochemistry & Molecular Biology
Deepti Singh, Pierre C. Dromel, Michael Young
MOLECULAR BIOLOGY REPORTS
(2020)
Article
Ophthalmology
Xiaoyu Chen, Deepti Singh, Ron A. Adelman, Lawrence J. Rizzolo
CURRENT EYE RESEARCH
(2020)
Letter
Surgery
Daniel J. Thomas, Deepti Singh
INTERNATIONAL JOURNAL OF SURGERY
(2020)
Article
Materials Science, Multidisciplinary
Pierre Colombe Dromel, Deepti Singh, Alfredo Alexander-Katz, Motoichi Kurisawa, Myron Spector, Michael Young
APPLIED MATERIALS TODAY
(2020)
Article
Cell & Tissue Engineering
Pierre C. Dromel, Deepti Singh, Ty Christoff-Tempesta, Tanisha Martheswaran, Alfredo Alexander-Katz, Myron Spector, Michael Young
Summary: This study compared the diffusion and release of hEGF in different injectable hydrogels and found that the normalized diffusion and release of hEGF increases with free water content but decreases with hydrogel stiffness. The experimental data was compared with theoretical diffusion models, with the hydrodynamic model providing the most suitable explanation for the measured solute diffusion coefficient.
TISSUE ENGINEERING PART A
(2021)
Letter
Surgery
Daniel J. Thomas, Deepti Singh
INTERNATIONAL JOURNAL OF SURGERY
(2021)
Article
Cell & Tissue Engineering
Pierre C. Dromel, Deepti Singh, Eliot Andres, Molly Likes, Motoichi Kurisawa, Alfredo Alexander-Katz, Myron Spector, Michael Young
Summary: The biomaterial-based cell replacement approach using gelatin-hyaluronic acid IPN shows promising potential in enhancing intravitreal retinal cell therapy. It improves cell viability, promotes regeneration, and reduces immune cell response, making it a viable treatment option worth further exploration and application in the future.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell Biology
Murilo Batista Abud, Petr Baranov, Sara Patel, Caroline A. Hicks, David Leonardo Cruvinel Isaac, Ricardo Noguera Louzada, Pierre Dromel, Deepti Singh, John Sinden, Marcos P. Avila, Michael Young
Summary: Despite efforts and advances, age-related macular degeneration remains a common cause of vision loss. This study compares the phenotypic profile of pig and human retinal progenitor cells (RPCs) and investigates the survival and integration of green fluorescent protein-pig RPCs after transplantation into pig eyes. The study establishes the phenotypic equivalency between porcine and human RPCs and suggests an initial dose for future human clinical trials. The findings indicate the potential of using pig models for cellular treatments in vision restoration.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Letter
Surgery
Daniel J. Thomas, Deepti Singh
INTERNATIONAL JOURNAL OF SURGERY
(2022)
Article
Polymer Science
Pierre C. Dromel, Deepti Singh, Alfredo Alexander-Katz, Motoichi Kurisawa, Myron Spector, Michael Young
Summary: Engineering matrices for cell therapy require design criteria that include the ability of these materials to support, protect, and enhance cellular behavior in vivo. The chemical and mechanical formulation of the biomaterials can influence not only target cell phenotype but also cellular differentiation. By manipulating the stiffness of the hydrogel, the differentiation potential of human retinal progenitor cells can be controlled.
Article
Cell & Tissue Engineering
Deepti Singh, Pierre C. Dromel, Tatiana Perepelkina, Petr Baranov, Michael Young
CELL TRANSPLANTATION
(2020)
Article
Ophthalmology
Deepti Singh, Xiaoyu Chen, Tina Xia, Maryam Ghiassi-Nejad, Laurel Tainsh, Ron A. Adelman, Lawrence J. Rizzolo
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Jeevan Srinivas Rajkumar, Michael Maddison, Deepti Singh, Pierre Colombe, Myron Spector, Michael J. Young
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Article
Materials Science, Biomaterials
Christopher Igwe Idumah, I. C. Nwuzor, S. R. Odera, U. J. Timothy, U. Ngenegbo, F. A. Tanjung
Summary: This paper presents recent advancements in drug delivery applications of polymeric hydrogel nanoarchitectures and hydrogel derivatives, including the incorporation of nanoparticulates and the enhancement of the chemical, biological, electrical, and mechanical properties of hydrogels. The application of magnetically responsive and smart hydrogels as drug delivery systems is also discussed.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2024)
Article
Materials Science, Biomaterials
Remya K. Rajan, Sunitha Chandran, Annie John, Ramesh Parameswaran
Summary: This study outlines the fabrication and characterization of pamidronate (PDS) embedded nanofibrous scaffolds based on polycaprolactone/polycaprolactone-polyethyleneglycol-polycaprolactone (PCL/CEC) blends for osteoporotic bone defect management. The electrospinning technique was utilized to fabricate PDS-loaded scaffolds, and the effect of PDS on the blends was evaluated in terms of surface morphology, wettability, biodegradability, and in vitro cytocompatibility. The results showed that the PCL/CEC-PDS scaffolds had a fibroporous architecture, appropriate mechanical properties, and the ability to support cell growth, indicating their potential for managing osteoporotic bone defects.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2024)
Article
Materials Science, Biomaterials
Kubra Aydin, Handan Sevim, Hatice Kaplan Can
Summary: This study aims to develop a new dental material by designing, synthesizing, and characterizing nanocomposites, and evaluate their cytotoxic effects on human gingival fibroblasts. The results of this research will contribute to a better understanding of the properties and safety of this material, providing reference for clinical applications in dentistry.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2024)
Review
Materials Science, Biomaterials
Angelica Jimenez-Rosales, Stefani Cortes-Camargo, Pedro Estanislao Acuna-Avila
Summary: The immune response to drug-delivering systems and implanted medical devices based on biomaterials is complex, and the goal is to design biomaterials that can respond to stimuli, preserve tissue function, and optimize cellular structures. Understanding the material's structure and location, as well as the severity of damage and other factors, is crucial in developing biocompatible biomaterials.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2024)
Article
Materials Science, Biomaterials
Fatemeh Daliri Shadmehri, Ehsan Karimi, Ehsan Saburi
Summary: Bone tissue engineering utilizes polymeric scaffolds and stem cells to create a supportive matrix resembling the in vivo environment for bone repair. The study found that a composite matrix of polycaprolactone and fibrin enhanced the differentiation of adipose-derived stem cells into osteo-like cells.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2024)