Article
Cell Biology
Dan Lei, Wen-Ting Yang, Peng-Sheng Zheng
Summary: HOXB4 suppresses cervical cancer cell proliferation and tumorigenesis by inhibiting the activity of the Wnt/beta-catenin signaling pathway.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Kornelia Lach, Aneta Kowal, Marta Perek-Polnik, Pawel Jakubczyk, Christopher J. Arthur, Wioletta Bal, Monika Drogosiewicz, Bozenna Dembowska-Baginska, Wieslawa Grajkowska, Jozef Cebulski, Radoslaw Chaber
Summary: This study evaluated the potential of FTIR spectroscopy as a diagnostic tool for medulloblastoma (MB). The results showed significant differences in FTIR spectra between MB and normal brain tissue, indicating that FTIR spectroscopy can be used to differentiate them to some extent. Therefore, it may be a valuable method to expedite and enhance histological diagnosis.
Review
Cell Biology
Veronica Marabitti, Manuela Giansanti, Francesca De Mitri, Francesca Gatto, Angela Mastronuzzi, Francesca Nazio
Summary: Tumor-specific alterations in metabolism are crucial for sustaining cell growth and survival in various cancers, but the heterogeneous nature of metabolism poses challenges for effective anticancer therapies. Medulloblastoma is a highly heterogeneous pediatric brain tumor with complex intratumoral cellular diversity. This review highlights the mechanisms through which medulloblastoma cells rewire their metabolism and energy production networks to support rapid growth, resistance to stress, invasion, metastasis, and therapy. The potential clinical benefits of targeting energy metabolism in suppressing medulloblastoma progression and enhancing current therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Matthew Thomas Ferreira, Juliano Andreoli Miyake, Renata Nascimento Gomes, Fabio Feitoza, Pollyana Bulgarelli Stevannato, Andrew Silva da Cunha, Fernanda de Oliveira Serachi, Alexandros Theodoros Panagopoulos, Alison Colquhoun
Summary: The study demonstrated the importance of PGE(2) signaling through the EP2 and EP4 receptors in controlling GBM cell biology, as well as a relationship between COX1 and MMP2 in these cells. These findings suggest a potential novel therapeutic target for drug development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Review
Oncology
Min Li, Yuhao Deng, Wangming Zhang
Summary: Medulloblastoma, the most common malignant brain cancer in pediatrics, is classified into four molecular subgroups. The identification of metastatic drivers has provided important insights into the treatment of medulloblastoma. The tumor microenvironment and signaling pathways play crucial roles in the spread and invasion of tumor cells.
MOLECULAR CANCER RESEARCH
(2021)
Article
Oncology
Jiaqing Yi, Xuanming Shi, Zhenyu Xuan, Jiang Wu
Summary: The study reveals the tumor suppressor functions of UTX in SHH medulloblastoma through mechanisms involving the activation of Th1-type chemokines and the impaired neural differentiation in cancerous cerebellar granule neuron precursors (CGNPs). The findings also suggest that UTX deletion can accelerate medulloblastoma development by coordinating with active SHH signaling.
Article
Oncology
Miriam Ratliff, Kianush Karimian-Jazi, Dirk C. Hoffmann, Laurel Rauschenbach, Matthias Simon, Ling Hai, Henriette Mandelbaum, Marc C. Schubert, Tobias Kessler, Stefanie Uhlig, Daniel Dominguez Azorin, Erik Jung, Matthias Osswald, Gergely Solecki, Mate E. Maros, Varun Venkataramani, Martin Glas, Nima Etminan, Bjoern Scheffler, Wolfgang Wick, Frank Winkler
Summary: This study used real-time 3D in vivo 2-photon laser scanning microscopy to longitudinally observe the invasion and proliferation of tumor cells. It found that highly invasive tumor cells also have high proliferative capability during brain colonization. The phenotype of tumor cells gradually transitions into slower-cycling glioblastoma cells.
Article
Multidisciplinary Sciences
Chiara Enrico Bena, Marco Del Giudice, Alice Grob, Thomas Gueudre, Mattia Miotto, Dimitra Gialama, Matteo Osella, Emilia Turco, Francesca Ceroni, Andrea De Martino, Carla Bosia
Summary: The study identified how the growth of cancer cell populations is impacted by the initial cell density, with higher initial densities leading to faster adaptation and a peak in population growth rate at intermediate densities. The findings contradict traditional logistic growth models and provide insight into the relationship between adaptation time, growth rate, and tumor take rates in cancer research.
SCIENTIFIC REPORTS
(2021)
Review
Oncology
Niek F. H. N. van Bree, Margareta Wilhelm
Summary: Medulloblastoma, the most common malignant childhood brain cancer, requires new treatment options that target the tumor without harming healthy tissue. Understanding the composition and interactions within the tumor microenvironment is crucial for better understanding and treating medulloblastoma.
Article
Biochemistry & Molecular Biology
Marieke Gringmuth, Jenny Walther, Sebastian Greiser, Magali Toussaint, Benjamin Schwalm, Marcel Kool, Rolf-Dieter Kortmann, Annegret Glasow, Ina Patties
Summary: In this study, an innovative therapy approach combining irradiation, decitabine, and abacavir was investigated to improve the survival of children with high-risk SHH/TP53-mut and Group 3 medulloblastoma. The therapy inhibited tumor growth and enhanced mouse survival without inducing adverse effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Antonia Harangus, Raduly Lajos, Livia Budisan, Oana Zanoaga, Cristina Ciocan, Cecilia Bica, Radu Pirlog, Ioan Simon, Marioara Simon, Cornelia Braicu, Ioana Berindan-Neagoe
Summary: This study evaluates miRNA expression patterns and specific mechanisms in male patients with NSCLC. The results identify several overexpressed and underexpressed miRNAs, as well as a common miRNA signature. Additionally, mRNA-miRNA regulatory networks were created to identify critical molecules and reveal NSCLC signaling pathways. These findings contribute to understanding the mechanisms involved in NSCLC development and offer opportunities for potential therapeutic targets.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Polymer Science
Petruta Preda, Ana-Maria Enciu, Bianca Adiaconita, Iuliana Mihalache, Gabriel Craciun, Adina Boldeiu, Ludmila Aricov, Cosmin Romanitan, Diana Stan, Catalin Marculescu, Cristiana Tanase, Marioara Avram
Summary: This study developed a new formulation of amorphous hydrogel and demonstrated its pro-proliferation and antibacterial properties. These hydrogels show potential as alternatives for wound healing.
Article
Multidisciplinary Sciences
Asmaa M. Fahim, Sawsan Dacrory, Ghada H. Elsayed
Summary: In this study, microcrystalline cellulose was oxidized to cellulose aldehyde using NaIO4 under microwave irradiation. The resulting cellulose aldehyde was further reacted with uracil acetamide to yield arylidene cellulose MDAU(4), which can react with nitrogen nucleophiles and cyclize to form pyrazole cellulose MDPA(5). The obtained cellulosic derivatives were characterized using FT-IR, NMR, and SEM. Cytotoxic activity analysis showed that compound MDAU(4) exhibited stronger inhibitory effect on the growth of A549 and Caco2 cancer cells compared to standard values. The expression levels of Wnt signaling cascade genes in A549 cells were also investigated and compound MDAU(4) was found to reduce the expression levels of these genes. Docking simulation was used to study the binding of uracil cellulosic heterocycles to different proteins.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Stacey M. Frumm, Shengyang Kevin Yu, Joseph Chang, Jordan A. Artichoker, Sonia M. Scaria, Katharine P. Lee, Lauren E. Byrnes, Julie B. Sneddon, Aaron D. Tward
Summary: Research revealed that the homeostatic mechanism of the tympanic membrane (TM) epidermis is distinct from other epidermal sites, with discrete proliferative zones containing multiple keratinocyte populations. TM stem cells in a specific location generate long-lived clones and CPs, while CP clones exhibit lateral migration with support from Pdgfra+ fibroblasts. This study provides insights into TM disorders and keratinocyte biology modeling.
Article
Hematology
Victoria Simionatto Zucherato, Pericles Natan Mendes da Costa, Marta Giovanetti, Luciana Maria Fontanari Krause, Daiani Cristina Ciliao Alves, Renata Maria Alencar Moreira, Barbara Maciel Sidou Pimentel, Rodrigo Haddad, Hellen Tayana Bitencourt, Massimo Ciccozzi, Luiz Carlos Junior Alcantara, Simone Kashima, Dimas Tadeu Covas, Svetoslav Nanev Slavov
Summary: The study evaluated the prevalence of MCPyV DNA among Brazilian blood donors and found an overall prevalence of 1.1%, with similar rates in different regions.
TRANSFUSION CLINIQUE ET BIOLOGIQUE
(2023)
Article
Public, Environmental & Occupational Health
Maria Carolina Elias, Svetoslav Nanev Slavov, Alex Ranieri Jeronimo Lima, Antonio Jorge Martins, Claudia Renata dos Santos Barros, Debora Botequio Moretti, Eduardo L. Araujo, Elaine Cristina Marqueze, Gabriela Ribeiro, Gabriela Mauric Frossard Ribeiro, Jardelina Souza Todao Bernardino, Jaqueline Reginato Koser, Luan Gaspar Clemente, Luiz Aurelio Campos Crispin, Luiz Carlos Alcantara Jr, Luiz Lehmann Coutinho, Marta Giovanetti, Quetura Oliveira Silva, Raul Machado Neto, Ricardo Haddad, Simone Kashima, Vincent Louis Viala, Dimas Tadeu Covas, Sandra Coccuzzo Sampaio
Summary: Brazil has been severely affected by the SARS-CoV-2 pandemic and has been a continuous source of virus variants. Due to insufficient infrastructure in some regions of the country, the Butantan Institute established a mobile laboratory (LabMovel) to conduct SARS-CoV-2 testing and monitor the pandemic in key areas.
Letter
Clinical Neurology
Matheus Ballestero, Ricardo Santos de Oliveira
CHILDS NERVOUS SYSTEM
(2023)
Article
Pediatrics
Mauricio Dutra, Stephanya Covas da Silva, Pamella da S. Beggiora, Marcelo V. Santos, Helio R. Machado, Luiza da S. Lopes
Summary: This study describes the epidemiological profile and socio-economic burden of hydrocephalus patients on the national public health system in Brazil using data from the online database of the Brazilian Health Ministry (DataSUS). The study found that hydrocephalus has a significant impact on public health budgets and pediatric mortality rates, but it is likely underestimated due to the lack of demographic data and epidemiological studies in Latin America, particularly in Brazil.
JORNAL DE PEDIATRIA
(2023)
Article
Multidisciplinary Sciences
Dimas Tadeu Covas, Ariane de Jesus Lopes de Abreu, Carolina Zampirolli Dias, Rafaela Vansan Ferreira, Ramon Goncalves Pereira, Guilherme Silva Julian
Summary: This study aims to describe the adverse events of COVID-19 vaccines in pregnant and postpartum women in Brazil. The results support the recommendation of vaccination for these groups, but further studies with longer observation time are still needed to provide more comprehensive safety evidence.
Article
Biotechnology & Applied Microbiology
Gabriela Munis Campos, Monique Ferrary Americo, Andria dos Santos Freitas, Fernanda Alvarenga Lima Barroso, Joyce da Cruz Ferraz Dutra, Ludmila Silva Quaresma, Barbara Fernandes Cordeiro, Juliana Guimaraes Laguna, Luis Claudio Lima de Jesus, Aparecida Maria Fontes, Alexander Birbrair, Tulio Marcos Santos, Vasco Azevedo
Summary: Using probiotics as vehicles for delivering therapeutic agents with anti-inflammatory properties through recombinant protein expression is a promising approach for preventing and treating various diseases, such as cancer and intestinal immune disorders.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Clinical Neurology
Felipe Gutierrez Pineda, Santiago Diaz Pinillos, Juan Pablo Gutierrez Pineda, Matheus Ballestero, Ricardo Santos De Oliveira
Summary: This study aimed to analyze the educational opportunities in pediatric neurosurgery in Latin America, assessing the strengths, weaknesses, and limitations of the field. An online survey was distributed to pediatric neurosurgeons in the region, providing insights into their education, working conditions, and training opportunities. The majority of the respondents had received their training in Latin American pediatric neurosurgery programs. There are 19 accredited academic programs in pediatric neurosurgery, located in 6 different countries in Latin America. The average duration of pediatric neurosurgical training in the region is 278 years, with a range of 1 to > 6 years. This study is the first of its kind to review pediatric neurosurgical training in Latin America, highlighting the dominance of certified pediatric neurosurgeons who were trained in the region. However, there are areas for improvement, such as the regulation of training opportunities, increased support for funding, and the provision of more educational opportunities for all countries.
CHILDS NERVOUS SYSTEM
(2023)
Article
Virology
Anielly S. da Silva, Gabriel Montenegro de Campos, Marta Giovanetti, Victoria Simonatto Zucherato, Alex Ranieri Jeronimo Lima, Elaine Vieira Santos, Rodrigo Haddad, Massimo Ciccozzi, Luiz Carlos Junior Alcantara, Maria Carolina Elias, Sandra Coccuzzo Sampaio, Dimas Tadeu Covas, Simone Kashima, Svetoslav Nanev Slavov
Summary: In this study, metagenomics was used to identify viral infections among pediatric patients with acute respiratory disease. Two pools of samples were found to contain the little-known Malawi polyomavirus (MWPyV). Further testing revealed that MWPyV-positive samples also tested positive for respiratory syncytial virus. Phylogenetic analysis of MWPyV genome sequences from index samples was conducted, providing insights into its viral origin. This study represents the first molecular and genomic characterization of MWPyV in pediatric patients in South America.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Oncology
Dalila Luciola Zanette, Karoline Brito Caetano Andrade Coelho, Eneas de Carvalho, Mateus Nobrega Aoki, Jeanine Marie Nardin, Larissa Araujo Lalli, Rafael dos Santos Bezerra, Marta Giovanetti, Victoria Simionatto Zucherato, Gabriel Montenegro de Campos, Jardelina de Souza Todao Bernardino, Vincent Louis Viala, Massimo Ciccozzi, Luiz Carlos Junior Alcantara, Sandra Coccuzzo Sampaio, Maria Carolina Elias, Simone Kashima, Dimas Tadeu Covas, Svetoslav Nanev Slavov
Summary: A study examined the composition of plasma virome in patients with prostate cancer, finding that Anelloviruses and Human Pegivirus-1 (HPgV-1) were the most abundant components. Clinically important viruses like hepatitis C virus (HCV), cytomegalovirus, and human adenovirus type C were also identified. In comparison, the virome of blood donors was mainly composed of torque teno virus types (TTV). This study sheds light on the viral abundance and diversity in patients with prostate cancer, suggesting that malignant processes may influence virome composition.
MOLECULAR & CELLULAR ONCOLOGY
(2023)
Review
Neurosciences
Rodrigo Inacio Pongeluppi, Matheus Fernando Manzolli Ballestero, Marcelo Volpon Santos, Ricardo Santos de Oliveira
Summary: Choroid plexus papillomas (CPPs) are rare benign neoplasms, particularly in the posterior fossa in children. We report a case series of five patients treated at a tertiary care hospital and conducted a comprehensive literature review. Gross total resection (GTR) was achieved in two patients initially, and all patients showed clinical improvement. The literature review included 27 articles published between 1975 and 2021, totaling 46 patients, with a mean age of 8.2 years.
ARQUIVOS DE NEURO-PSIQUIATRIA
(2023)
Article
Environmental Sciences
Barbara Gimenes de Castro, Bruno Mari Fredi, Rafael dos Santos Bezerra, Queren Apuque Alcantara, Carlos Eduardo Milani Neme, Daniele Enriquetto Mascarelli, Aline Seiko Carvalho Tahyra, Douglas dos-Santos, Camilla Rizzo Nappi, Fernanda Santos de Oliveira, Flavia Pereira Freire, Giulia Ballestero, Julia Beatriz Menuci Lima, Juliana de Andrade Bolsoni, Juliana Lourenco Gebenlian, Naira Lopes Bibo, Nathalia Soares Silva, Nilton de Carvalho Santos, Victoria Simionatto Zucherato, Kamila Chagas Peronni, Daniel Guariz Pinheiro, Emmanuel Dias-Neto, Gilberto Gambero Gaspar, Valdes Roberto Bollela, Vanessa da Silva Silveira, Aparecida Maria Fontes, Nilce Maria Martinez-Rossi, Svetoslav Nanev Slavov, Joao Paulo Bianchi Ximenez, Fernando Barbosa, Wilson Araujo Silva Jr
Summary: High-resolution 16S rRNA profiling can identify a high diversity of bacteria in both indoor and outdoor environments of a hospital. The most common bacterial genera associated with nosocomial infections include Cutibacterium, Streptococcus, Staphylococcus, and Corynebacterium. The study suggests that external areas may directly influence the microbial community in the pediatric ICU. These results provide valuable insights for the development of bacterial genomic surveillance and decontamination policies to reduce nosocomial infections.
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
(2023)
Article
Hematology
Victoria Simionatto Zucherato, Marta Giovanetti, Lara Okuyama Afonso Costa, Luciana Maria Fontanari Krause, Daiani Cristina Ciliao Alves, Renata Maria Alencar Moreira, Barbara Maciel Sidou Pimentel, Rodrigo Haddad, Hellen Tayana Bitencourt, Massimo Ciccozzi, Luiz Carlos Alcantara Jr, Simone Kashima, Dimas Tadeu Covas, Svetoslav Nanev Slavov
Summary: This study investigated the prevalence of HuGkV-2 DNA among healthy blood donors from different regions in Brazil. The results showed that the highest prevalence was recorded in the Brazilian Amazon at 15.3%. Although this provides preliminary insights into the impact of this emerging viral agent, further studies are needed to understand the prevalence, transmission routes, and possible clinical effects.
TRANSFUSION AND APHERESIS SCIENCE
(2023)
Letter
Clinical Neurology
Matheus Ballestero, Ricardo Santos de Oliveira
ACTA NEUROCHIRURGICA
(2023)
Article
Clinical Neurology
Matheus Ballestero, Celeste Dias, Ines Catarina Neves Gomes, Luca Soares Grisi, Rodrigo Augusto Monteiro Cardoso, Edson Luis Zucoloto Junior, Ricardo Santos de Oliveira
Summary: This study aimed to compare the noninvasive Brain4Care system with invasive ICP curve parameters in predicting ICH and functional prognosis in severe TBI. The results showed that the noninvasive parameters were not associated with outcomes or ICH. Conclusion: Brain4Care's noninvasive method showed low specificity and accuracy and cannot be used as the sole means of monitoring ICP in patients with severe TBI.
ACTA NEUROCHIRURGICA
(2023)
Review
Neurosciences
Rodrigo Inacio Pongeluppi, Matheus Fernando Manzolli Ballestero, Marcelo Volpon Santos, Ricardo Santos de Oliveira
Summary: Choroid plexus papillomas (CPPs), particularly in the posterior fossa in children, are rare. This study presents a case series of five patients and includes a comprehensive literature review. Gross total resection surgery is the preferred treatment and hydrocephalus is common and requires prompt treatment.
ARQUIVOS DE NEURO-PSIQUIATRIA
(2023)