Article
Biochemistry & Molecular Biology
Austin McGinnis, Vladimir I. Klichko, William C. Orr, Svetlana N. Radyuk
Summary: The research found that the oxidation state of peroxiredoxins changes under oxidative stress conditions, and that genetic mutations leading to reduced catalytic activity of peroxiredoxins do not affect the survival ability of fruit flies, but may instead increase their physiological activity.
Review
Biochemistry & Molecular Biology
Izabela Sadowska-Bartosz, Grzegorz Bartosz
Summary: Peroxiredoxin 2 (Prdx2) is an abundant erythrocyte protein that acts as an antioxidant, removing hydrogen peroxide and other peroxides. It can also serve as a chaperone for hemoglobin and erythrocyte membrane proteins.
Article
Biology
Jiao Meng, Yuanyuan Wang, Zhenyu Lv, Xinhua Qiao, Aojun Ye, Qiaoli Zhu, Chang Chen
Summary: Aging is closely related to redox regulation, and the decline in redox-stress response capacity (RRC) is a dynamic characteristic of aging. Peroxiredoxin 2 (PRDX2), serving as a sensor for hydrogen peroxide (H2O2), mediates RRC decline during aging. PRDX2 knockdown leads to decreased RRC and accelerated senescence in fibroblasts, while prdx-2 mutant C. elegans also show reduced RRC. The hyperoxidation of PRDX2 in senescent cells is responsible for the decreased sensor activity and reduced cellular response to H2O2.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Review
Endocrinology & Metabolism
Jennifer S. Stancill, John A. Corbett
Summary: Oxidative stress is implicated in pancreatic beta-cell damage in type 1 and type 2 diabetes, potentially leading to dysfunction and death of beta cells. Beta cells are vulnerable to oxidative damage but have high expression of antioxidant enzymes which may help maintain their function and survival.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Multidisciplinary Sciences
Yuichi Yokochi, Yuka Fukushi, Ken-ichi Wakabayashi, Keisuke Yoshida, Toru Hisabori
Summary: Thioredoxin and its related proteins play crucial roles in chloroplasts, regulating and coordinating the redox state of various photosynthesis-related proteins to adapt to the constantly changing light environment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Immunology
Amber Dorey, Krystyna Cwiklinski, James Rooney, Carolina De Marco Verissimo, Jesus Lopez Corrales, Heather Jewhurst, Barbara Fazekas, Nichola Eliza Davies Calvani, Siobhan Hamon, Siobhan Gaughan, John P. Dalton, Richard Lalor
Summary: Research has shown that the Prx1 and Trx1 of Fasciola hepatica play important roles in stress responses, not only acting as antioxidants, but also modulating host immune responses and inflammation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Azahara Casatejada, Leonor Puerto-Galan, Juan M. Perez-Ruiz, Francisco J. Cejudo
Summary: Oxidizing signals mediated by thiol-dependent peroxidase activity play a crucial role in chloroplast redox balance, with both 2-Cys peroxiredoxins (PRXs) and glutathione peroxidases (GPXs) being involved. However, the specific contribution of GPXs in this process is not well understood. This study generated mutant plants devoid of GPXs to investigate their functional relationship with the NADPH-dependent thioredoxin reductase C (NTRC)-2-Cys PRXs redox system. The results indicate that GPXs contribute to chloroplast redox homeostasis independently of NTRC.
Review
Biochemistry & Molecular Biology
Stefano Caserta, Pietro Ghezzi
Summary: Redox enzymes, such as thioredoxin and peroxiredoxin, have been found to act as soluble mediators in immunity by being released from cells. Peroxiredoxins, in particular, can be secreted via exosome-release route but the exact mechanism and reasons behind this process remain unclear. This review discusses the potential redox states of protein cysteines and the role of redox enzymes in their regulation, as well as the studies on the extracellular forms of these enzymes and their association with exosomes/extracellular vesicles in infection and inflammation.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Ewelina A. Klupczynska, Karl-Josef Dietz, Arleta Malecka, Ewelina Ratajczak
Summary: Mitochondria play a crucial role in various biological processes, such as energy metabolism, cell signaling, and cellular metabolism. Oxidative stress disrupts mitochondrial redox homeostasis and decreases seed viability, but proteins like PRXIIF, TRX, and GPXL are involved in protecting cells, sensing oxidative stress, and maintaining redox balance.
Review
Biochemistry & Molecular Biology
Garett J. Steers, Gloria Y. Chen, Brianne R. O'Leary, Juan Du, Hannah Van Beek, Joseph J. Cullen
Summary: Pancreatic cancer is a significant global health issue with limited progress in treatment development. Pharmacologic ascorbate (P-AscH(-)) and auranofin (Au) show promise as potential therapies for pancreatic cancer.
Article
Biochemistry & Molecular Biology
Sebastian F. Villar, Laura Corrales-Gonzalez, Belen Marquez de los Santos, Joaquin Dalla Rizza, Ari Zeida, Ana Denicola, Gerardo Ferrer-Sueta
Summary: The reduction reactions of cytosolic human peroxiredoxins (Prx) in their disulfide form by thioredoxins (Trx) were studied. The reactivity of the nucleophilic cysteine in Trx was found to be greatly affected by the formation of the Prx-Trx complex. This activation mechanism helps understand the reduction of Prx by alternative reductants involved in redox signaling.
Article
Agriculture, Dairy & Animal Science
Walid S. Habashy, Marie C. Milfort, Romdhane Rekaya, Samuel E. Aggrey
Summary: Performance traits and mortality of chickens are negatively impacted by heat stress, with the thioredoxin pathway system potentially offering clues for nutritional strategies to mitigate these effects.
Article
Chemistry, Physical
Lukas Lang, Ann-Cathrin Wolf, Mareike Riedel, Lea Thibol, Fabian Geissel, Kristina Feld, Jannik Zimmermann, Bruce Morgan, Georg Manolikakes, Marcel Deponte
Summary: 1-Cys Prx is an enzyme that uses only one cysteine residue for the reduction of hydroperoxides, and it requires an external thiol for the reduction of a reactive sulfenic acid. This study compared the reaction mechanisms and kinetics of Prx5 and Prx6 enzymes from Plasmodium falciparum to investigate the hyperoxidation susceptibility and potential substrate promiscuity of 1-Cys Prx.
Article
Biochemistry & Molecular Biology
Mojca Trstenjak Prebanda, Petra Matjan-Stefin, Boris Turk, Natasa Kopitar-Jerala
Summary: The study investigated the impact of lipopolysaccharide-triggered neuroinflammation on the protein levels of redox-sensitive proteins in the brain and cerebella of Stefin B-deficient mice. The results suggest a role of oxidative stress in the progression of the disease.
Article
Oncology
Chelsey D. Kline, Madeleine Anderson, John W. Bassett, Gail Kent, Rachel Berryman, Matthew Honeggar, Shosuke Ito, Kazumasa Wakamatsu, Arup K. Indra, Philip J. Moos, Sancy A. Leachman, Pamela B. Cassidy
Summary: Melanomas and melanocytes are affected by reactive oxygen species (ROS), and the antioxidant enzyme thioredoxin reductase-1 (TR1) plays a role in controlling the stability and function of the master regulator MITF. TR1 and selenoproteins have paradoxical effects in melanocytes and melanomas, but increasing selenoprotein activity can prevent oxidative damage and tumor formation. Depletion of TR1 leads to oxidation of MITF and has implications for pigmentation and tumorigenesis in melanocyte lineage cells.
Article
Multidisciplinary Sciences
Tomokazu Fukuda, Kai Furuya, Kouhei Takahashi, Ai Orimoto, Eriko Sugano, Hiroshi Tomita, Sayo Kashiwagi, Tohru Kiyono, Tsuyoshi Ishii
Summary: This study compared the biological characteristics and genome-wide expression profiles of immortalized human dermal papilla cells generated by different methods to determine the optimal immortalization method. The data would be valuable to scientists aiming to establish new immortalized cell lines.
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Takeshi Iwamoto, Ken Itoh, Hiroshi Tomita, Taku Ozaki
Summary: The study revealed that mitochondrial calpain-5 is activated under low Ca2+ concentrations and in response to endoplasmic reticulum stress, indicating its therapeutic potential in diseases associated with ER stress.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Kai Furuya, Tao Wu, Ai Orimoto, Eriko Sugano, Hiroshi Tomita, Tohru Kiyono, Takahiro Kurose, Yoshihiro Takai, Tomokazu Fukuda
Summary: The study investigated the expression patterns of immortalized corneal epithelial cells with different genetic modifications, showing that SV40 immortalized cells had a distinct expression pattern from wild-type cells regardless of serum presence, while corneal epithelial cells with combinatorial expression had a pattern closer to that of wild-type cells.
Article
Biochemistry & Molecular Biology
Kitako Tabata, Eriko Sugano, Akito Hatakeyama, Yoshito Watanabe, Tomoya Suzuki, Taku Ozaki, Tomokazu Fukuda, Hiroshi Tomita
Summary: The study demonstrated that retinal ganglion cells were photosensitive after the transduction of optogenetic genes and did not induce any phototoxicity following exposure to continuous light.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Izzah Munirah, Taku Ozaki, Aya Sekine, Motoko Morimoto, Mayu Sugawara, Haruka Takada, Eriko Sugano, Hiroshi Tomita, Tohru Kiyono, Tomokazu Fukuda
Summary: Dogs are considered as the first animals to establish close relationship with humans, exhibiting unique genetic evolution and diversity. Domestic dogs are used in safety tests of pharmacological products, emphasizing the importance of considering the welfare of experimental animals. In this study, immortalized domestic dog-derived fibroblasts were successfully established as a potential in vivo model system for evaluating pharmacological chemicals without the need for animal testing.
Article
Cell Biology
Haruka Takada, Tomisato Miura, So Fujibayashi, Naomi Sasaki, Kouhei Takahashi, Eriko Sugano, Hiroshi Tomita, Taku Ozaki, Tohru Kiyono, Mitsuaki A. Yoshida, Tomokazu Fukuda
Summary: Cell immortalization allows for infinite expansion, but may alter the nature of the original cells. This study found that immortalized cells often exhibit chromosome abnormalities, while introducing CDK4, cyclin D1, and TERT can better maintain the original chromosome condition.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2021)
Article
Cell & Tissue Engineering
Yoshito Watanabe, Eriko Sugano, Kitako Tabata, Akito Hatakeyama, Tetsuya Sakajiri, Tomokazu Fukuda, Taku Ozaki, Tomoya Suzuki, Tatsuki Sayama, Hiroshi Tomita
Summary: The research team has successfully increased the light sensitivity of optogenetic gene-mediated therapy by designing mutants, which were used to restore visual responses in rats with genetically degenerating photoreceptor cells. The results suggest that ex3mV1Co may be an effective method for restoring advanced visual function.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell Biology
Takenori Oikawa, Tomokazu Fukuda, Tetsuro Yamashita, Hiroshi Tomita, Taku Ozaki
Summary: The study shows that Tat-mu CL has a protective effect, effectively inhibiting glutamate-induced cell death and preventing nuclear translocation of AIF and DNA fragmentation. Therefore, Tat-mu CL may be a potential gene therapy modality for neurodegenerative diseases.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2022)
Article
Biochemistry & Molecular Biology
Yuki Kikuchi, Eriko Sugano, Shiori Yuki, Kitako Tabata, Yuka Endo, Yuya Takita, Reina Onoguchi, Taku Ozaki, Tomokazu Fukuda, Yoshihiro Takai, Takahiro Kurose, Koichi Tanaka, Yoichi Honma, Eduardo Perez, Maxwell Stock, Jose R. Fernandez, Masanori Tamura, Michael Voronkov, Jeffry B. Stock, Hiroshi Tomita
Summary: SIG-1451 partially protects against light-induced photoreceptor degeneration by inhibiting inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Daiki Sakai, Hiroshi Tomita, Akiko Maeda
Summary: Optogenetics is a breakthrough in neuroscience with potential applications in treating retinal degenerative diseases. Clinical trials are currently underway to restore vision using optogenetic therapy. Choosing the right optogenetic tools and gene delivery methods is crucial for post-treatment vision improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Himari Matsusaka, Tao Wu, Kai Furuya, Tomoe Yamada-Kato, Lanlan Bai, Hiroshi Tomita, Eriko Sugano, Taku Ozaki, Tohru Kiyono, Isao Okunishi, Tomokazu Fukuda
Summary: This study identified downstream target genes involved in testosterone signalling in DPCs through RNA-Seq analysis, providing important data for the discovery of molecular targets for prostate cancer and AGA.
Article
Biochemistry & Molecular Biology
Akito Hatakeyama, Eriko Sugano, Tatsuki Sayama, Yoshito Watanabe, Tomoya Suzuki, Kitako Tabata, Yuka Endo, Tetsuya Sakajiri, Tomokazu Fukuda, Taku Ozaki, Hiroshi Tomita
Summary: Channelrhodopsins have been used in gene therapy to restore vision in patients with retinitis pigmentosa. The channel kinetics of ComV1 variants with different amino acid residues at the 172nd position were investigated. The study found that the characteristics of the amino acid at this position affect the radius of the ion gate, thereby altering the photocurrent and channel kinetics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuya Takita, Eriko Sugano, Kazuya Kitabayashi, Kitako Tabata, Akito Saito, Takanori Yokoyama, Reina Onoguchi, Tomokazu Fukuda, Taku Ozaki, Lanlan Bai, Hiroshi Tomita
Summary: This study used multifocal electroretinography to evaluate focal retinal function in rats exposed to continuous light. The results showed a significant decrease in retinal function after 24 hours of light exposure, with a regional dependency in retinal damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Kai Furuya, So Fujibayashi, Tao Wu, Kouhei Takahashi, Shin Takase, Ai Orimoto, Eriko Sugano, Hiroshi Tomita, Sayo Kashiwagi, Tohru Kiyono, Tsuyoshi Ishii, Tomokazu Fukuda
Summary: Testosterone signaling affects the focal adhesion pathway by regulating AR gene expression, with caveolin-1 as a target of AR regulation. This study provides important data resources for the discovery of new therapeutic targets for testosterone-related diseases.
Article
Biochemistry & Molecular Biology
Tetsuya Sakajiri, Masatoshi Nakatsuji, Yoshiaki Teraoka, Kosuke Furuta, Katsuya Ikuta, Kotoe Shibusa, Eriko Sugano, Hiroshi Tomita, Takashi Inui, Takaki Yamamura
Summary: The study investigates the interaction mechanism between ceruloplasmin and transferrin mediated by zinc. The findings suggest that zinc may play a role in the transfer of iron between ceruloplasmin and transferrin.
