Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 101, Issue -, Pages 823-836Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.03.093
Keywords
Black cumin; Gastric ulcer; Pectic polysaccharide
Funding
- Department of Science and Technology, New Delhi
- Department of Biotechnology, New Delhi
- Council of Scientific and Industrial Research, India
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Gastric ulcer is a multi-step disease and healing requires a complex process including repair and re architecture of gastric mucosa with the involvement of molecular events. Current study was designed to understand the gastric ulcer healing mechanism of rhamnogalacturonan-I type pectic polysaccharide of black cumin (BCPP) utilizing acetic acid induced gastric ulcers in rats. BCPP fed groups at 200 mg/kg b.w. for 10 days showed up to 85% healing of gastric ulcers with modulation of key molecular events involved in ulcer healing process such as increase in gastric mucin content, cyclooxygenase-2 (Cox-2) and prostaglandin E-2 (PGE(2)). The increase in extracellular signal-regulated kinase-2 (ERK-2) indicated that, BCPP could induce PGE-2 synthesis by increasing ERIC-2 mediated COX-2 activity. Increase in matrix metalloproteinase-2 (MMP-2) and decrease in MMP-9 levels in BCPP treated groups indicated differential regulation of MMP-2 and 9, an essential event required for gastric mucosal re-modulation. BCPP containing bound phenolics (26 mg/g) might have also played a role in increasing speed and quality of ulcer healing by inhibiting H+, K+-ATPase and decreasing free radical mediated oxidation and cellular damages. Overall, studies showed that the polysaccharide can mediate ulcer healing by modulating signaling pathways involved in either ulcer aggravation or healing process. (C) 2017 Published by Elsevier B.V.
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