Journal
CURRENT MICROBIOLOGY
Volume 72, Issue 2, Pages 152-158Publisher
SPRINGER
DOI: 10.1007/s00284-015-0932-5
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Funding
- National Natural Science Foundation of China [31302131]
- Research Fund for the Doctoral Program of Higher Education of China [20135103120006]
- China Postdoctoral Science Foundation [2014M552378]
- National science and technology support program [2015BAD12B05]
- National Special Fund for Agro-scientific Research in the Public Interest [201003012]
- China Agricultural Research System [CARS-43-8]
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Riemerella anatipestifer (R. anatipestifer) is among the most prevalent duck pathogens, causing acute or chronic septicemia characterized by serositis. Riemerella anatipestifer can be grown on blood-enriched media, in vitro, which provides a hemin source essential for the sustainment of R. anatipestifer and activation of hemin-uptake systems. However, the genes associated with hemin uptake cannot be identified exclusively through genome sequence analysis. Here, we show that R. anatipestifer encodes outer-membrane hemin-binding proteins. Hemin-binding proteins were identified in the cytoplasm with apparent molecular mass of similar to 45/37/33/23/20/13 kDa, and outer membrane with apparent molecular mass of similar to 90/70/60/50/15 kDa by batch affinity chromatography and hemin-blotting assays. Our results indicate that these proteins are involved in hemin acquisition. Finally, hemin-binding assay further showed that R. anatipestifer can bind hemin and this capability is increased in iron limited medium, indicating the hemin-uptake system of R. anatipestifer was regulated by iron.
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