Article
Chemistry, Analytical
Zhongyue Guo, Yeran Bai, Meng Zhang, Lu Lan, Ji-Xin Cheng
Summary: Antimicrobial resistance poses a major threat to global health and economics, necessitating rapid AST methods. We introduce a rapid AST method based on wide-field mid-infrared photothermal imaging, capable of metabolic imaging for hundreds of bacteria at single-cell resolution within seconds. This method enables probing of perturbed microbial protein synthesis within 1 hour after antibiotic treatment in E. coli cells, providing insights into antibiotic susceptibility.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jared J. Lodico, Matthew Mecklenburg, Ho Leung Chan, Yueyun Chen, Xin Yi Ling, B. C. Regan
Summary: By using electron energy-loss spectroscopy (EELS) technique in a scanning transmission electron microscope (STEM), we demonstrate operando spectrum imaging of a Li-ion battery anode, allowing us to observe the constituents and structures of the solid-electrolyte interphase (SEI) layer. This provides insights into the complex mechanisms affecting battery safety, capacity, and lifetime.
Article
Nanoscience & Nanotechnology
Kirill Kniazev, Evgenii Zaitsev, Shubin Zhang, Yang Ding, Loc Ngo, Zhuoming Zhang, Gregory V. Hartland, Masaru Kuno
Summary: Label-free, bond-selective imaging has new opportunities in chemistry, biology, and material science. However, issues such as low sensitivity, low spatial and temporal resolution hinder its application in studying spatially-congested specimens. In this paper, a widefield infrared photothermal heterodyne imaging technique (wIR-PHI) is introduced, which overcomes these issues and enables high-resolution imaging with hyperspectral capabilities and high sensitivity. The technique has potential for kinetic imaging and spectroscopic studies in important chemical, biological, and material processes in the future. Rating: 9/10.
Article
Optics
Alejandro De la Cadena, Federico Vernuccio, Andrea Ragni, Giuseppe Sciortino, Renzo Vanna, Carino Ferrante, Natalia Pediconi, Carlo Valensise, Luca Genchi, Sergey P. P. Laptenok, Andrea Doni, Marco Erreni, Tullio Scopigno, Carlo Liberale, Giorgio Ferrari, Marco Sampietro, Giulio Cerullo, Dario Polli
Summary: Spontaneous Raman microscopy can reveal the chemical composition of a sample in a non-invasive manner but suffers from slow imaging speed. Stimulated Raman scattering amplifies the signal and enables high-speed imaging. Researchers have developed a broadband stimulated Raman scattering microscope that simultaneously measures signals at 32 frequencies, allowing for high-resolution mapping of spectrally congested samples.
Article
Multidisciplinary Sciences
Seth Kenkel, Mark Gryka, Lin Chen, Matthew P. Confer, Anirudha Rao, Scott Robinson, Kannanganattu V. Prasanth, Rohit Bhargava
Summary: Nearfield spectroscopic imaging techniques can be a powerful tool to simultaneously map cellular ultrastructure and molecular composition, but their current capabilities are limited. This study proposes an instrument design that combines null-deflection measurements with resonance enhancement to achieve high-sensitivity nanoscale infrared imaging. Experimental results using cellular acini samples demonstrate the ability of this method to easily record high-quality chemical imaging data.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Mikael Marois, Jonathan D. Olson, Dennis J. Wirth, Jonathan T. Elliott, Xiaoyao Fan, Scott C. Davis, Keith D. Paulsen, David W. Roberts
Summary: A system for rapid hyperspectral imaging during neurosurgery has been developed, which can acquire data from 64 spectral channels simultaneously. The system uses a birefringent spectral demultiplexer to split incoming light and redirects it to different sections of a large format microscope sensor. This new device is an important advance in achieving real-time quantitative imaging of fluorophore concentration for guiding surgery.
COMMUNICATIONS BIOLOGY
(2023)
Article
Optics
Yujie Cai, Yu Chen, Xiaoning Xin, Kun Huang, E. Wu
Summary: This study demonstrates a broadband mid-infrared (MIR) single-photon frequency upconversion spectroscopy method. By utilizing the temporal-spectral quantum correlation of non-degenerate photon pairs, the signal is extracted from the noise caused by strong pump, achieving single-photon-level MIR spectral measurement. The system features non-destructive and robust operation, making it an appealing option for biochemical applications.
PHOTONICS RESEARCH
(2022)
Article
Construction & Building Technology
Eberechi Ichi, Sattar Dorafshan
Summary: The presence of fouling contamination in railroad ballast is a growing concern that leads to deterioration, poor drainage functionality, and premature failure of railroad components. This study presents the results of characterizing laboratory ballast samples using hyperspectral imaging (HSI) sensor, showing the potential of HSI in detecting, monitoring, and quantifying moisture content (MC) and fouling content (FC) in fouled ballast. The reflectance of ballast samples with different levels of MC and FC was determined, and the presence of water was found to be prominent in the 1350 nm to 1550 nm wavelength range.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Review
Multidisciplinary Sciences
Yeran Bai, Jiaze Yin, Ji-Xin Cheng
Summary: Mid-infrared photothermal microscopy overcomes the limitations of traditional infrared spectroscopic imaging by using visible light to detect the photothermal effect induced by infrared absorption. It provides submicrometer spatial resolution with high spectral fidelity and reduced water background, thus offering a wide range of applications in life sciences and materials.
Article
Chemistry, Analytical
Kate Sendin, Marena Manley, Federico Marini, Paul J. Williams
Summary: This study aimed to develop an accurate and affordable automated analytical technique for white maize grading using NIR spectral imaging in the South African maize industry. Through 25 PLS-DA models, the 17 categories and sub-categories were classified in a hierarchical decision pathway, achieving an overall accuracy of 93.3% for the main categories. Three waveband selection techniques were employed, with the VIP set based on only 7.3% of the original spectral variables recommended as the best trade-off between performance and cost.
MICROCHEMICAL JOURNAL
(2021)
Review
Optics
Longqian Huang, Ruichen Luo, Xu Liu, Xiang Hao
Summary: This article reviews state-of-the-art deep-learning-empowered computational spectral imaging methods, which possess fast reconstruction speed, excellent reconstruction quality, and the potential to reduce system volume significantly.
LIGHT-SCIENCE & APPLICATIONS
(2022)
Article
Chemistry, Analytical
Costanza Cucci, Andrea Casini, Lorenzo Stefani, Barbara Cattaneo, Marcello Picollo
Summary: This work presents a new prototype of a transmittance hyperspectral imaging scanner designed for non-invasive analysis of photographic materials. The instrument provides high-quality spectral data and high-definition images on small targets and is the first of its kind specifically designed for photographic conservation. Experimental results demonstrate the feasibility of using hyperspectral imaging for large-scale spectroscopic characterization of photographic archival materials, encouraging further experimentation in photography conservation.
Article
Computer Science, Information Systems
Pradip Salve, Pravin Yannawar, Milind Sardesai
Summary: Automatic classification of plants using image processing and machine learning techniques based on leaf venation patterns and spectral signatures can achieve high accuracy, with feature fusion technique outperforming non-imaging spectral signatures features. This approach shows promise for efficient plant identification.
JOURNAL OF KING SAUD UNIVERSITY-COMPUTER AND INFORMATION SCIENCES
(2022)
Article
Chemistry, Analytical
Thulya Chakkumpulakkal Puthan Veettil, Diana Alves, Jitraporn Vongsvivut, Rosemary L. Sparrow, Bayden R. Wood, Gil Garnier
Summary: Oxidative treatment of RBCs prior to freeze-drying stabilizes the cells for storage at room temperature. Synchrotron-based FTIR microspectroscopy was used to analyze single RBCs and compare lipid and protein spectral data before and after oxidation and freeze-drying. The analysis showed that oxidized and freeze-dried RBCs have different spectral profiles compared to untreated RBCs, indicating changes in lipid peroxidation, stiffness of the membrane, and protein secondary structure. Freeze-drying did not introduce additional changes. This study highlights the potential of freeze-dried RBCs as a stable source for blood serology testing and demonstrates the power of synchrotron FTIR microspectroscopic live-cell analysis.
Article
Chemistry, Analytical
Ziling Jiang, Xianli Wang, Kaiqin Chu, Zachary J. Smith
Summary: Low-resolution Raman spectroscopy combined with context-aware matrix completion significantly improves the speed and image quality of Raman imaging without sacrificing chemical specificity or spatial image quality.
Article
Immunology
Imran Uraizee, Vishal K. Varma, Hari Sreedhar, Francesca Gambacorta, Shaiju S. Nazeer, Aliya Husain, Michael J. Walsh
Article
Computer Science, Interdisciplinary Applications
Neeraj Kumar, Phanikrishna Uppala, Karthik Duddu, Had Sreedhar, Vishal Varma, Grace Guzman, Michael Walsh, Amit Sethi
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2019)
Article
Biochemical Research Methods
Camilo L. M. Morais, Maria Paraskevaidi, Li Cui, Nigel J. Fullwood, Martin Isabelle, Kassio M. G. Lima, Pierre L. Martin-Hirsch, Hari Sreedhar, Julio Trevisan, Michael J. Walsh, Dayi Zhang, Yong-Guan Zhu, Francis L. Martin
Article
Chemistry, Multidisciplinary
Resmi Nair, Lakshmi Nair, Divya Maldepalli Govindachar, Hema Santhakumar, Shaiju S. Nazeer, Charuvil Radhakrishnapillai Rekha, Sachin J. Shenoy, Ganga Periyasamy, Ramapurath S. Jayasree
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Chemistry, Analytical
Hari Sreedhar, Mary Carns, Kathleen Aren, Shaiju S. Nazeer, Michael J. Walsh, John Varga
VIBRATIONAL SPECTROSCOPY
(2020)
Article
Biochemical Research Methods
Vidyani Suryadevara, Shaiju S. Nazeer, Hari Sreedhar, Oluwatobi Adelaja, Andre Kajdacsy-Balla, Viswanathan Natarajan, Michael J. Walsh
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Spectroscopy
S. Ramesh, Shaiju S. Nazeer, Sunila Thomas, V. Vivek, Ramapurath S. Jayasree
Summary: Oral lichen planus (OLP) is a chronic inflammatory condition that may develop into malignancy. Autofluorescence spectroscopy (AFS) can be used for non-invasive diagnosis, with 320nm light identified as the optimal wavelength for excitation.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Suha Mohiuddin, Siva Sreedhar, Hari Sreedhar, David Martinez, Osayd Nazzal, Ron C. Gaba, Michael J. Walsh
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY
(2021)
Article
Dermatology
Nikki Kuhar, Shaiju S. Nazeer, Rekha V. Kumar, Geetashree Mukherjee, Siva Umapathy
Summary: The study successfully differentiated early-stage oral hyperplasia from adjacent normal tissues and oral squamous cell carcinoma using Fourier transform infrared imaging technology. Spectral analysis revealed characteristics such as an increase in DNA content, protein denaturation, and altered lipid structures. This study demonstrates a simple strategy for using Fourier transform infrared imaging technology to differentiate early-stage oral hyperplasia, adjacent normal tissues, and oral squamous cell carcinoma.
LASERS IN SURGERY AND MEDICINE
(2021)
Article
Spectroscopy
Shaiju S. Nazeer, T. P. Sreedevi, Ramapurath S. Jayasree
Summary: This study investigated the impact of liver fibrosis on other organs such as the heart, lungs, kidneys, and spleen using fluorescence spectroscopy. Different excitation wavelengths revealed variations in endogenous fluorophores, and multivariate analysis distinguished control from fibrosis-induced groups. The findings suggest that fluorescence emission spectral study, in combination with multivariate analysis, can help identify biochemical alterations in other organs due to the development of liver fibrosis, potentially leading to tissue damage and dysfunction.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Shaiju S. Nazeer, Ariya Saraswathy, Nirmala Nimi, Elangovan Sarathkumar, A. N. Resmi, Sachin J. Shenoy, Ramapurath S. Jayasree
Summary: This study presents the development of magneto-fluorescent hybrid nanoparticles that combine iron oxide and carbon nanoparticles for dual-functional fluorescence and magnetic resonance imaging.
METHODS AND APPLICATIONS IN FLUORESCENCE
(2023)
Article
Chemistry, Analytical
J. Sacharz, D. Perez-Guaita, Mustafa Kansiz, Shaiju S. Nazeer, A. Weselucha-Birczynska, S. Petratos, B. R. Wood, P. Heraud
ANALYTICAL METHODS
(2020)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
